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1.
Zhonghua Wai Ke Za Zhi ; 61(6): 474-480, 2023 Jun 01.
Artigo em Zh | MEDLINE | ID: mdl-37088479

RESUMO

Objective: To examine the safety and effectiveness of laparoscopic individualized surgical treatment for chronic traumatic diaphragmatic hernia (CTDH). Methods: The clinical data and follow-up data of 29 CTDH cases admitted to the Qilu Hospital of Shandong University or the First Affiliated Hospital of Shandong First Medical University from June 2015 to January 2023 were retrospectively analyzed. There were 21 males and 8 females, aged (49.4±17.8) years (range: 19 to 79 years). The main clinical manifestations were symptoms of the digestive system and respiratory system, and only 4 cases were asymptomatic. All patients received laparoscopic treatment (conversion to open surgery was not excluded). Intraoperative exploration (location of the hernia, contents of the hernia, diameter of the hernia ring), surgical conditions (surgical repair plan, operation time, blood loss, postoperative complications) and postoperative follow-up were analyzed and discussed. Results: Laparoscopic repair was successfully completed in 27 cases, including simple suture in 6 cases, suture and patch repair in 17 cases, the anterior abdominal wall muscle flap reversal suture and patch repair of in 3 cases, and patch bridge repair in 1 case. The operation time was (112.8±44.7) minutes (range: 60 to 200 minutes). The amount of bleeding (M(IQR)) was 35 (58) ml (range: 10 to 300 ml). The other 2 patients were converted to laparotomy. Except for one patient with transverse colon strangulation necrosis who died of aggravated pulmonary infection after surgery, the remaining 28 patients were discharged successfully. The follow-up time was 36 (24) months (range: 1 to 60 months). During the follow-up period, only two patients had occasional left upper abdominal discomfort. Twenty-seven patients with left diaphragmatic hernia had no recurrence, and the symptoms of 1 patient with right diaphragmatic hernia were relieved. Conclusion: Customized laparoscopic surgical repair for CTDH according to the location and size of the diaphragmatic defect can achieve good surgical results.


Assuntos
Hérnia Diafragmática Traumática , Laparoscopia , Masculino , Feminino , Humanos , Hérnia Diafragmática Traumática/cirurgia , Estudos Retrospectivos , Laparoscopia/métodos , Complicações Pós-Operatórias , Laparotomia , Telas Cirúrgicas
2.
Genet Mol Res ; 14(4): 13049-65, 2015 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-26535618

RESUMO

Karyopherins, including alpha and beta types, are transport proteins in the eukaryotic cell that carry cargoes across nuclear pore complexes into or out of the nucleus. In this study, full open reading frames of one beta and three alpha types of karyopherin were cloned from cDNA of the domestic silkworm (Bombyx mori). The one beta and three alpha types' open reading frames were 2661, 1563, 1515, and 1551 base pairs long, respectively, and coded 886, 520, 504, and 516 amino acids, respectively. The alphas all had one importin-beta-binding (IBB) domain, and eight, four, or seven armadillo/beta-catenin-like repeats. The beta had 19 HEAT repeat domains, which constructed one importin-beta-N-terminal domain and one IBB domain. The recombinant proteins were expressed in Escherichia coli cells. The molecular weight of the beta type was approximately 100 kDa, and the alphas weighed approximately 60 kDa. Phylogenic tree construction revealed that the alphas could be classified into three known karyopherin-alpha subfamilies. We detected mRNA of the four karyopherins in normal 3rd day of 5th instar larvae, and in larvae injected with Gram-positive bacteria, Gram-negative bacteria, viruses, and fungi using real-time fluorescence quantitative reverse transcriptase-polymerase chain reaction, and found that the four karyopherins were widely distributed, but their expression levels were related to tissues type, the microbe injected, and the time point.


Assuntos
Bombyx/genética , Bombyx/imunologia , Expressão Gênica , Imunidade , Carioferinas/genética , Sequência de Aminoácidos , Animais , Sequência de Bases , Bombyx/classificação , Clonagem Molecular , Carioferinas/química , Carioferinas/metabolismo , Dados de Sequência Molecular , Fases de Leitura Aberta , Especificidade de Órgãos/genética , Filogenia , RNA Mensageiro/genética , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Análise de Sequência de DNA
3.
AIDS Res Hum Retroviruses ; 17(15): 1423-33, 2001 Oct 10.
Artigo em Inglês | MEDLINE | ID: mdl-11679155

RESUMO

HIV-1 cardiomyopathy has become a major cause of death in AIDS patients, but its pathogenesis is unclear. We used an antigen retrieval technique and immunostaining to investigate the hearts of 15 AIDS patients, of whom 3 had dilated cardiomyopathy. Immunocytochemistry shows infiltration of the left ventricular myocardium with mononuclear cells, ranging from minimal to diagnostic of myocarditis. The infiltrates include macrophages and CD3(+) and CD8(+) T cells. The tight junction protein ZO-1 is disrupted at the site of monocyte-macrophage vascular penetration and the coronary vessels show fibrinogen leakage in the hearts of AIDS patients, but not in the normal heart. A subset of infiltrating macrophages is doubly positive for cyclooxygenase 2 (COX-2) and inducible nitric oxide synthase. HIV-1 peptides gp120 and Nef are expressed in macrophages and T cells, but not in cardiomyocytes. COX-2 is expressed by both gp120-positive and gp120-negative macrophages. The hearts of AIDS patients separate into those showing minimal infiltrates with low COX-2 expression and those with dense infiltrates and high COX-2; all failing hearts are in the latter group. These data suggest that COX-2-activated and HIV-1-infected monocyte-macrophages and T cells play a crucial role in the progression of HIV-1 myocarditis to HIV-1 cardiomyopathy.


Assuntos
Infecções por HIV/enzimologia , HIV-1/fisiologia , Isoenzimas/fisiologia , Ativação Linfocitária/imunologia , Ativação de Macrófagos/imunologia , Macrófagos/imunologia , Miocardite/imunologia , Prostaglandina-Endoperóxido Sintases/fisiologia , Linfócitos T/imunologia , Disfunção Ventricular Esquerda/imunologia , Encéfalo/imunologia , Vasos Coronários/imunologia , Ciclo-Oxigenase 2 , Infecções por HIV/complicações , Infecções por HIV/imunologia , Infecções por HIV/virologia , Humanos , Isoenzimas/metabolismo , Rim/imunologia , Leucócitos/imunologia , Fígado/imunologia , Macrófagos/virologia , Proteínas de Membrana , Miocardite/complicações , Miocardite/enzimologia , Miocardite/virologia , Óxido Nítrico Sintase/metabolismo , Óxido Nítrico Sintase Tipo II , Prostaglandina-Endoperóxido Sintases/metabolismo , Linfócitos T/virologia , Disfunção Ventricular Esquerda/complicações , Disfunção Ventricular Esquerda/enzimologia , Disfunção Ventricular Esquerda/virologia
4.
Chin Med J (Engl) ; 103(7): 546-51, 1990 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-2119971

RESUMO

Severe acute pancreatitis is highly controversial on its diagnostic criteria, the optimum time for surgery, the selection of surgical procedures, and the prevention and treatment of complications. We treated 40 patients with severe acute pancreatitis from July 1983 to July 1988. The comparison of clinical and laboratory data of severe acute pancreatitis and mild acute pancreatitis showed that in some patients neither Ranson's nor Bank's criteria are reliable in classifying or predicting the severity of the disease. The coexistence of acute peritonitis and bloody ascites with elevated amylase level is very helpful to identify the local conditions of pancreatic necrosis and hemorrhage. We suggest early operation (within 48 hours) be applied in severe acute pancreatitis. In our series, five types of surgical procedures were used. We consider that proper treatment of acute respiratory distress syndrome (ARDS) is most important in the management of severe pancreatitis.


Assuntos
Pancreatite/diagnóstico , Abscesso/etiologia , Doença Aguda , Adolescente , Adulto , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Pancreatite/complicações , Pancreatite/cirurgia , Prognóstico , Síndrome do Desconforto Respiratório/etiologia , Estudos Retrospectivos
5.
Eur J Clin Invest ; 32(5): 360-71, 2002 May.
Artigo em Inglês | MEDLINE | ID: mdl-12027877

RESUMO

BACKGROUND: Monocyte/macrophages are known to infiltrate the brain of patients with HIV-1 encephalitis (HIVE). In Alzheimer's disease brain, the origin of activated microglia has not been determined. MATERIALS AND METHODS: We employed the antigen retrieval technique, immunocytochemistry, immunofluorescense, and confocal microscopy to identify macrophages and microglia in relation to amyloid-beta plaques and the blood-brain barrier in autopsy brain tissues from patients with Alzheimer's disease (AD) and HIVE. RESULTS: In both conditions, cyclooxygenase-2 positive macrophages and, to a lesser degree, T and B cells infiltrate brain perivascular spaces and neuropil. The macrophages are distinguishable from ramified microglia, and decorate the vessels at the sites of apparent of endothelial tight junction protein ZO-1 disruption. The macrophages also infiltrate amyloid-beta plaques, display intracellular amyloid-beta and are surrounded by amyloid-beta-free lacunae. Furthermore, the macrophages partially encircle the walls of amyloid-beta-containing vessels in amyloid angiopathy, and exhibit intracellular amyloid-beta but not paracellular lacunae. Significantly larger zones of fibrinogen leakage surround the microvessels in HIVE brain tissues compared with AD tissues (P = 0.034), and AD tissues have significantly greater leakage than control tissues (P = 0.0339). The AD group differs from a normal control age-matched group with respect to both the area occupied by CD68 (P = 0.03) and cyclooxygenase-2 immunoreactive cells (P = 0.004). CONCLUSION: In both HIVE and AD, blood-borne activated monocyte/macrophages and lymphocytes appear to migrate through a disrupted blood-brain barrier. The lacunae around macrophages in amyloid-beta plaques but not in vessel walls are consistent with the ability of macrophages to phagocytize and clear amyloid-beta deposits in vitro.


Assuntos
Complexo AIDS Demência/imunologia , Doença de Alzheimer/imunologia , Antígenos CD/metabolismo , Antígenos de Diferenciação Mielomonocítica/metabolismo , Barreira Hematoencefálica , Encéfalo/metabolismo , Isoenzimas/metabolismo , Macrófagos/fisiologia , Prostaglandina-Endoperóxido Sintases/metabolismo , Complexo AIDS Demência/metabolismo , Complexo AIDS Demência/patologia , Adulto , Idoso , Idoso de 80 Anos ou mais , Doença de Alzheimer/metabolismo , Doença de Alzheimer/patologia , Peptídeos beta-Amiloides/metabolismo , Antígenos CD/imunologia , Antígenos de Diferenciação Mielomonocítica/imunologia , Encéfalo/patologia , Circulação Cerebrovascular , Ciclo-Oxigenase 2 , HIV-1 , Humanos , Imuno-Histoquímica , Linfócitos/fisiologia , Masculino , Proteínas de Membrana , Pessoa de Meia-Idade , Fragmentos de Peptídeos/metabolismo , Junções Íntimas/metabolismo
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