RESUMO
In recent years, long non-coding RNAs have emerged as a novel class of regulators of cancer biological processes. While they are dysregulated in many cancer types, little is known about their expression and functional profiles. This study has been focused on the determination of the role of a specific lncRNA in papillary thyroid cancer. Quantitative reverse transcription PCR was performed to detect the expression levels of 84 lncRNAs in 61 papillary thyroid carcinoma tissues and their adjacent non-tumor tissues. The highest fold-change was obtained for lung cancer associated transcript 1 LUCAT1, and thus, this study determines the expression and biological implication of lncRNA LUCAT1 through different in vitro and ex vivo approaches in this tumor. LUCAT1 was specifically located at the cell nucleus in tumoral regions of patient tissues. Furthermore, LUCAT1 knockdown significantly reduced both cell proliferation and invasion ex vivo and induced cell-cycle arrest and apoptosis. These facts were corroborated by an enhanced expression of P21, P57, P53 and BAX, and a reduced expression of EZH2 and HDAC1. In addition, a significant decrease was observed on DNMT1 and NRF2 genes, helping to clarify the role of LUCAT1 on PTC. Our study reveals the involvement of LUCAT1 in PTC development, through acting in cell-cycle regulation, proliferation, epigenetic modifications through LUCAT1/ CDK1/ EZH2/ P57/ P21/ HDAC1/ DNMT1/ P53/ BAX axis and apoptosis, via extrinsic pathway activating caspases. These findings indicate that LUCAT1 is maybe a potential therapeutic target and molecular biomarker for PTC.
Assuntos
Biomarcadores Tumorais/genética , RNA Longo não Codificante/genética , Câncer Papilífero da Tireoide/genética , Apoptose/genética , Proliferação de Células/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Masculino , Pessoa de Meia-Idade , Proteínas de Neoplasias/genética , Transdução de Sinais/genética , Câncer Papilífero da Tireoide/patologiaRESUMO
Compounds present in wastewater olive oil (WWOO) which can be used in metabolic pathways of Azotobacter chroococcum (A. chroococcum) have been investigated. Some compounds such as syringic acid, p-coumaric acid and syringaldehyde do not favour microorganism growing up. However, it has been shown that in batch culture, polyphenolic compounds (PCs) such as protocatetic acid and p-hydroxybenzoic acid do facilitate the growing up of microorganism. What is more, the maximum concentration in which bacteria can grow was 0.3% (w/v) for both polyphenols. At higher concentrations, substrate inhibition was observed; which is characterized by decreasing growth rates. Therefore, A. chroococcum can grow up using PCs as an individual source of carbon and energy supply but it is also dependent on the type of the compound and on its concentration. A gas chromatography coupled mass spectrometry method has been used for the study of the degradation of simple phenolic compounds.
Assuntos
Azotobacter/metabolismo , Cromatografia Gasosa-Espectrometria de Massas/métodos , Resíduos Industriais , Fenóis/metabolismo , Óleos de Plantas/metabolismo , Azeite de Oliva , Padrões de ReferênciaRESUMO
A gas chromatographic-mass spectrometric (GC-MS) method for qualitative and subsequent quantitative analysis of phenolic antioxidants compounds, presents in olive oil, in rat cerebrospinal fluid (CSF) after oral administration of compounds is proposed. The procedure involves the extraction of compounds from the samples by a traditional microliquid-liquid extraction method, followed by a silylation step before the GC-MS analysis. The chromatographic separation was performed by using a low bleed DB5-MS fused-silica capillary column. The presence of 21 phenolic compounds was tested in CSF extracts and only free tyrosol, hydroxytyrosol and ferulic acid were detected. Those compounds were then quantitatively determined using the proposed methology. The molecular ion for silylated compounds appears at 370 m/z for hydroxytyrosol, 282 m/z for tyrosol and 338 m/z for ferulic acid respectively, while the base peak appears at 267 m/z, 179 m/z and 338 m/z. alpha-Naphthol was used as a surrogate (216 and 201 m/z). The detection capabilities obtained were 74, 92 and 79 ng/mL respectively. The method was applied to the determination of trace amounts of compounds in rat cerebrospinal fluid after oral administration. The animals were fed with a standard chow diet (free of phenolic antioxidants) in order to avoid the influence of any other component of the diet on the CSF of the animals.