RESUMO
MDM2, a ubiquitin E3-ligase of the RING family, has a key role in regulating p53 abundance. During normal non-stress conditions p53 is targeted for degradation by MDM2. MDM2 can also target itself and MDMX for degradation. MDMX is closely related to MDM2 but the RING domain of MDMX does not possess intrinsic E3-ligase activity. Instead, MDMX regulates p53 abundance by modulating the levels and activity of MDM2. Dimerization, mediated by the conserved C-terminal RING domains of both MDM2 and MDMX, is critical to this activity. Here we report the crystal structure of the MDM2/MDMX RING domain heterodimer and map residues required for functional interaction with the E2 (UbcH5b). In both MDM2 and MDMX residues C-terminal to the RING domain have a key role in dimer formation. In addition we show that these residues are part of an extended surface that is essential for ubiquitylation in trans. This study provides a molecular basis for understanding how heterodimer formation leads to stabilization of MDM2, yet degradation of p53, and suggests novel targets for therapeutic intervention.
Assuntos
Estrutura Quaternária de Proteína , Proteínas Proto-Oncogênicas c-mdm2/química , Ubiquitina/metabolismo , Sequência de Aminoácidos , Cristalografia por Raios X , Dimerização , Humanos , Modelos Moleculares , Dados de Sequência Molecular , Proteínas Proto-Oncogênicas c-mdm2/genética , Proteínas Proto-Oncogênicas c-mdm2/metabolismo , Alinhamento de Sequência , Proteína Supressora de Tumor p53/metabolismoRESUMO
Control of apoptotic signalling pathways depends on the balance between proapoptotic and prosurvival molecules. The 'inhibitor of apoptosis' (IAP) proteins are negative regulators of apoptosis that function by inhibiting the executioners of cell death (caspases), or by blocking the pathways that activate them. The IAP proteins function as ubiquitin E3 ligases and possess protein-protein interaction domains. IAPs can promote the addition of ubiquitin to themselves and to the substrate proteins that they interact with either directly or indirectly through adaptor proteins. The balance between substrate and autoubiquitylation seems to be important for their activity. In this review, we describe the structural features of IAP proteins as they are currently understood, and discuss how each domain contributes to IAP function. It is clear that to advance our understanding of these complex proteins, we must decipher how the domains operate in concert and how each domain impacts on the activity of the other.