[PECULIAR PROPERTIES OF SOME COMPONENTS OF MORPHOLOGICAL STRUCTURE IN A PLANT CELL VACUOLE REVEALED BY CONFOCAL MICROSCOPY].

Results of investigations of peculiar properties related to the structure of plant cell vacuolar membranes are discussed. The study was carried out using confocal microscopy, which allowed us in the process of scanning to identify membrane tubes and vesicules in the preparations of isolated vacuoles. Such membrane tubes were found both inside and outside the vacuoles, and, in the case of scanning intermittently at equal time intervals, transition of vesicles with the membrane tube was observed. Furthermore, scanning of isolated vacuoles was conducted at various distances from the glass substrate. Each time, in the upper area of the isolated vacuole lying on the substrate, we observed a large segment of vacuolar membrane and registered the effect of highly intensive fluorescing of some of membrane segments. The distributions of laurdan fluorescence generalized polarization (GP) values for the vacuolar membrane on the whole and for the intensively fluorescing membrane segments have been obtained. We have found that the microviscosity of the intensively fluorescing membrane segments essentially differs from that of the rest part of the membrane.

Synthesis, structure, spectral and luminescence studies of novel guanidinium aryl(oxy)(sulfanyl)(sulfonyl)acetates.

A series of promising luminescent materials, nonlinear optical crystals, and physiologically active compounds - aryl(oxy)(sulfanyl)(sulfonyl)acetates of guanidine (A) of unknown type was synthesized. Various functional groups present in (A) were identified using FTIR spectroscopy. 1H and 13C NMR spectral studies further confirm the molecular structure (A). Crystals of guanidinium 4-chlorophenyl(sulfanyl)acetate (1) and guanidinium 4-chlorophenyl(sulfonyl)acetate (2) were successfully grown. They belong to the same lowest symmetry category, but to different crystal systems: monoclinic (1) and orthorhombic (2). It has been established that intrinsic optical absorption begins at a wavelength of âˆ¼ 290 nm for crystalline compound (1) and âˆ¼ 335 nm for crystal (2). The intrinsic luminescence spectrum of crystal (1) includes two bands with maxima at 300 and 515 nm. In the intrinsic luminescence spectrum of crystal (2), only one band is observed with a maximum at 350 nm. Such luminescence in both crystals is excited in the intrinsic absorption bands, as well as by X-ray radiation. In addition, in the near ultraviolet and throughout the visible region, where optical absorption is not detected (it is very weak), low-inertia (less than 10 ns) rather intense luminescence of uncontrolled impurity-defect centers is excited. The spectral bands of optical absorption, photo- and X-ray luminescence discovered in experiments were systematized using a diagram of energy levels and quantum transitions in crystals and defect centers of the compounds under study.