Phenotiki: an open software and hardware platform for affordable and easy image-based phenotyping of rosette-shaped plants.

Phenotyping is important to understand plant biology, but current solutions are costly, not versatile or are difficult to deploy. To solve this problem, we present Phenotiki, an affordable system for plant phenotyping that, relying on off-the-shelf parts, provides an easy to install and maintain platform, offering an out-of-box experience for a well-established phenotyping need: imaging rosette-shaped plants. The accompanying software (with available source code) processes data originating from our device seamlessly and automatically. Our software relies on machine learning to devise robust algorithms, and includes an automated leaf count obtained from 2D images without the need of depth (3D). Our affordable device (~€200) can be deployed in growth chambers or greenhouse to acquire optical 2D images of approximately up to 60 adult Arabidopsis rosettes concurrently. Data from the device are processed remotely on a workstation or via a cloud application (based on CyVerse). In this paper, we present a proof-of-concept validation experiment on top-view images of 24 Arabidopsis plants in a combination of genotypes that has not been compared previously. Phenotypic analysis with respect to morphology, growth, color and leaf count has not been performed comprehensively before now. We confirm the findings of others on some of the extracted traits, showing that we can phenotype at reduced cost. We also perform extensive validations with external measurements and with higher fidelity equipment, and find no loss in statistical accuracy when we use the affordable setting that we propose. Device set-up instructions and analysis software are publicly available ( http://phenotiki.com).

The significance of image compression in plant phenotyping applications.

We are currently witnessing an increasingly higher throughput in image-based plant phenotyping experiments. The majority of imaging data are collected using complex automated procedures and are then post-processed to extract phenotyping-related information. In this article, we show that the image compression used in such procedures may compromise phenotyping results and this needs to be taken into account. We use three illuminating proof-of-concept experiments that demonstrate that compression (especially in the most common lossy JPEG form) affects measurements of plant traits and the errors introduced can be high. We also systematically explore how compression affects measurement fidelity, quantified as effects on image quality, as well as errors in extracted plant visual traits. To do so, we evaluate a variety of image-based phenotyping scenarios, including size and colour of shoots, leaf and root growth. To show that even visual impressions can be used to assess compression effects, we use root system images as examples. Overall, we find that compression has a considerable effect on several types of analyses (albeit visual or quantitative) and that proper care is necessary to ensure that this choice does not affect biological findings. In order to avoid or at least minimise introduced measurement errors, for each scenario, we derive recommendations and provide guidelines on how to identify suitable compression options in practice. We also find that certain compression choices can offer beneficial returns in terms of reducing the amount of data storage without compromising phenotyping results. This may enable even higher throughput experiments in the future.