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The wake-mediated propulsion of an "extra" particle in a channel of two neighboring rows of a two-dimensional plasma crystal, observed experimentally by Du et al. [Phys. Rev. E 89, 021101(R) (2014)PRESCM1539-375510.1103/PhysRevE.89.021101], is explained in simulations and theory. We use the simple model of a pointlike ion wake charge to reproduce this intriguing effect in simulations, allowing for a detailed investigation and a deeper understanding of the underlying dynamics. We show that the nonreciprocity of the particle interaction, owing to the wake charges, is responsible for a broken symmetry of the channel that enables a persistent self-propelled motion of the extra particle. We find good agreement of the terminal extra-particle velocity with our theoretical considerations and with experiments.
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A theory of the mode-coupling instability (MCI) in a fluid two-dimensional complex plasma is developed. In analogy to the point-wake model of the wake-mediated interactions commonly used to describe MCI in two-dimensional crystals, the layer-wake model is employed for fluids. It is demonstrated that the wake-induced coupling of wave modes occurs in both crystalline and fluid complex plasmas, but the confinement-density threshold, which determines the MCI onset in crystals, virtually disappears in fluids. The theory shows excellent qualitative agreement with available experiments and provides certain predictions to be verified.
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AIM: To study the effects exerted by argon microwave nonthermal plasma (NTP) on cell wall-lacking Mollicutes bacteria. METHODS AND RESULTS: 10(8) CFU ml(-1) agar plated Mycoplasma hominis and Acholeplasma laidlawii were treated with the nonthermal microwave argon plasma for 30-300 s. The maximal 10- and 100-fold drop was observed for A. laidlawii and Myc. hominis, respectively. Similarly treated Escherichia coli and Staphylococcus aureus demonstrated the 10(5) and 10(3) drop, respectively. Removal of cholesterol affected resistance of A. laidlawii. 10 mmol l(-1) antioxidant butylated hydroxytoluene decreased mortality by a factor of 25-200. UV radiation alone caused 25-85% mortality in comparison with the whole NTP. Exogenously added hydrogen peroxide H2O2 did not cause mortality. NTP treatment of Myc. hominis triggered growth of microcolonies, which were several tenfold smaller than a typical colony. CONCLUSIONS: Despite the lack of cell wall, A. laidlawii and Myc. hominis were more resistant to argon microwave NTP than other tested bacteria. Mycoplasma hominis formed microcolonies upon NTP treatment. A role of UV and active species was demonstrated. SIGNIFICANCE AND IMPACT OF THE STUDY: The first study of NTP effects on Mollicutes revealed importance of a membrane composition for bacterial resistance to NTP. New specific Myc. hominis morphological forms were observed. The study confirmed importance of the concerted action of reactive oxygen species (ROS) with UV and other plasma bioactive agents for NTP bactericidal action.
Assuntos
Acholeplasma laidlawii/efeitos dos fármacos , Antibacterianos/farmacologia , Mycoplasma hominis/efeitos dos fármacos , Gases em Plasma/farmacologia , Argônio , Colesterol/fisiologia , Viabilidade Microbiana/efeitos dos fármacos , Micro-Ondas , Mycoplasma hominis/crescimento & desenvolvimento , Mycoplasma hominis/ultraestrutura , Oxidantes/farmacologia , Raios UltravioletaRESUMO
Experimental evidence is presented for a scale-free transition from an unordered to an ordered state in a 2D complex plasma that differs from the KTHNY theory of phase transitions in 2D systems. The transition is characterized by the formation and growth of ordered domains. A fractal relationship is found between the domain areas and domain boundary lengths, which can be explained by a recent theoretical model. The experimental findings are supported by a molecular dynamics simulation of a 2D particle system.
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In the past few years, cold atmospheric plasma (CAP) has evolved into a new tool in the fight against nosocomial infections and antibiotic-resistant microorganisms. The products generated by the plasma-electrons, ions, reactive species and UV light-represent a 'lethal cocktail' for different kinds of pathogen, which opens up possible applications in hygiene and medicine. Nevertheless, to ensure the safe usage of CAP on skin (e.g., to treat wounds or skin diseases) several pre-clinical in vitro studies have to be performed before implementing clinical trials on humans. In the study presented here, inactivation experiments with Escherichia coli were carried out to identify the necessary plasma dosage for a 5 log reduction: with a small hand-held battery-operated CAP device, these disinfection properties were achieved after application during 30s. This and higher plasma dosages were then used to analyze the mutagenicity induced in V79 Chinese hamster cells-to furthermore define a 'safe application window'-with the HPRT (hypoxanthine-guanine phosphoribosyl transferase) mutation assay. The results show that a CAP treatment of up to 240 s and repeated treatments of 30s every 12h did not induce mutagenicity at the Hprt locus beyond naturally occurring spontaneous mutations.
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Desinfecção/métodos , Escherichia coli/genética , Gases em Plasma/toxicidade , Esterilização/métodos , Ar , Animais , Linhagem Celular , Cricetinae , Cricetulus , Dano ao DNA , Desinfecção/instrumentação , Relação Dose-Resposta a Droga , Avaliação Pré-Clínica de Medicamentos , Hipoxantina Fosforribosiltransferase/genética , Íons , Testes de Mutagenicidade , Mutação , Espécies Reativas de Nitrogênio , Espécies Reativas de Oxigênio , Esterilização/instrumentação , Raios UltravioletaRESUMO
The microstructure of a strongly coupled liquid undergoing a shear flow was studied experimentally. The liquid was a shear melted two-dimensional plasma crystal, i.e., a single-layer suspension of micrometer-size particles in a rf discharge plasma. Trajectories of particles were measured using video microscopy. The resulting microstructure was anisotropic, with compressional and extensional axes at around ±45° to the flow direction. Corresponding ellipticity of the pair correlation function g(r) or static structure factor S(k) gives the (normalized) shear rate of the flow.
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The structure of Mach cones in a crystalline complex plasma has been studied experimentally using an intensity sensitive imaging, which resolved particle motion in three dimensions. This revealed a previously unknown out-of-plane cone structure, which appeared due to excitation of the vertical wave mode. The complex plasma consisted of micron sized particles forming a monolayer in a plasma sheath of a gas discharge. Fast particles, spontaneously moving under the monolayer, created Mach cones with multiple structures. The in-plane cone structure was due to compressional and shear lattice waves.
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AIM: Study microbicidal activity of low temperature argon plasma (LTP) that is a stream of partially ionized argon having macroscopic temperature of the environment against Chlamydia trachomatis obligate intracellular parasites. Study viability of host cells in parallel. MATERIALS AND METHODS: McCoy line cells infected with C. trachomatis (Bu-434/L2 strain) were exposed to LTP obtained by using atmospheric pressure plasma SHF generator. Intracellular localization of chlamydiae was visualized by luminescent microscopy. RESULTS: Exposure of infected McCoy line cells resulted in the destruction of chlamydia inclusions and practically complete elimination of intracellular bacteria. At the same time LTP exposure did not result in immediate death of host cells, an insignificant reduction of the number of cells was observed 24 hours after the exposure to LTP. CONCLUSION: The effect of LTP for elimination of intracellular chlamydia without significant changes in viability of eukaryotic host cells was demonstrated.
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Chlamydia trachomatis/crescimento & desenvolvimento , Gases em Plasma , Argônio/química , Contagem de Células , Linhagem Celular , Sobrevivência Celular , Infecções por Chlamydia/microbiologia , Humanos , Viabilidade Microbiana , Microscopia de Fluorescência , Especificidade da Espécie , TemperaturaRESUMO
A simple method of characterization of suspensions of spherical nanoparticles with monotonically variable size is proposed. It allows for the in situ measurement of the particle size as well as spectral dependence of their refractive indices. The method requires three optical channels: one for the illumination of a suspension by white light and two for the measurements of the spectra of scattered light. Parameters of the particles are determined by fitting the measured temporal spectral surfaces by the calculated Mie scattering functions. The method is applied to the particles being grown in a low-pressure reactive plasma of a discharge in an acetylene-argon mixture.
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The speed-stress relation for gliding edge dislocations was experimentally measured for the first time. The experimental system used, a two-dimensional plasma crystal, allowed observation of individual dislocations at the "atomistic" level and in real time. At low applied stress dislocations moved subsonically, at higher stress their speed abruptly increased to 1.9 times the speed of shear waves, then slowly grew with stress. There is evidence that immediately after nucleation dislocations can move faster than pressure waves.
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Low-pressure room-temperature neon, argon, krypton, and air plasmas were studied in magnetic fields up to flux densities of 2.3 T. Filaments appeared parallel to the magnetic field lines, and patterns such as spirals and concentric circles formed in the perpendicular direction. We link these effects to the magnetization of the ions. We also used a layer of embedded microparticles as probes in the plasma. Their motion changed dramatically from a collective rotation of the whole ensemble in moderate magnetic fields to a rotation in several small vortices centered at the filaments.
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Campos Magnéticos , Gases em Plasma/química , Pressão , TemperaturaRESUMO
Dedicated experiments with strongly coupled complex plasmas in external electric fields were carried out under microgravity conditions using the PK-4 dc discharge setup. The focus was put on the comparative analysis of the formation of stringlike anisotropic structures due to reciprocal (hamiltonian) and nonreciprocal (non-hamiltonian) interactions between microparticles (induced by ac and dc fields, respectively). The experiments complemented by numerical simulations demonstrate that the responses of complex plasmas in these two regimes are drastically different. It is suggested that the observed difference is a manifestation of intrinsic thermodynamic openness of driven strongly coupled systems.
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Freezing and melting of large three-dimensional complex plasmas under microgravity conditions is investigated. The neutral gas pressure is used as a control parameter to trigger the phase changes: Complex plasma freezes (melts) by decreasing (increasing) the pressure. The evolution of complex plasma structural properties upon pressure variation is studied. Theoretical estimates allow us to identify the main factors responsible for the observed behavior.
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Results of application of LTP at atmospheric pressure as an antibacterial agent during the last decade are considered with reference to physicochemical mechanisms of its bactericidal action. The principles of designing modern LTP sources are described in conjunction with the results of LTP application against pathogenic bacteria in vitro and in biofilms. The possibility to destroy biofilm matrix by LTP is estimated along with the results of its testing for the treatment of acute and chronic wound surfaces. Prospects for the development of "plasma medicine" in this country and abroad are discussed with special emphasis on its advantages, such as the absence of long-acting toxic compounds, small probability of spontaneous mutations accounting for resistance to LTP, relatively low cost of LTP sources, independence of LTP effect of the surface relief, painless application.
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Anti-Infecciosos Locais/farmacologia , Anti-Infecciosos Locais/uso terapêutico , Antissepsia , Gases em Plasma , Infecção dos Ferimentos , Animais , Antissepsia/instrumentação , Antissepsia/métodos , Biofilmes/efeitos dos fármacos , Ensaios Clínicos como Assunto , Contagem de Colônia Microbiana , Bactérias Gram-Negativas/efeitos dos fármacos , Bactérias Gram-Positivas/efeitos dos fármacos , Humanos , Testes de Sensibilidade Microbiana , Gases em Plasma/farmacologia , Gases em Plasma/uso terapêutico , Cicatrização/efeitos dos fármacos , Infecção dos Ferimentos/tratamento farmacológico , Infecção dos Ferimentos/microbiologiaRESUMO
Dedicated experiments on melting of two-dimensional plasma crystals were carried out. The melting was always accompanied by spontaneous growth of the particle kinetic energy, suggesting a universal plasma-driven mechanism underlying the process. By measuring three principal dust-lattice wave modes simultaneously, it is unambiguously demonstrated that the melting occurs due to the resonance coupling between two of the dust-lattice modes. The variation of the wave modes with the experimental conditions, including the emergence of the resonant (hybrid) branch, reveals exceptionally good agreement with the theory of mode-coupling instability.
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Using experiments and combining theory and computer simulations, we show that binary complex plasmas are particularly good model systems to study the kinetics of fluid-fluid demixing at the "atomistic" (individual particle) level. The essential parameters of interparticle interactions in complex plasmas, such as the interaction range(s) and degree of nonadditivity, can be varied significantly, which allows systematic investigations of different demixing regimes. The critical role of competition between long-range and short-range interactions at the initial stage of the spinodal decomposition is discussed.
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Systems with aligned anisotropic interactions between particles exhibit numerous phase transitions. A remarkable example of the fluid phase transition occurring in such systems is the formation of particle strings--the so-called "string" or "chain" fluids. We employ an approach based on the Ornstein-Zernike (OZ) equation, which allows us to calculate structural properties of fluids with aligned anisotropic interactions. We show that the string-fluid transition can be associated with the bifurcation of the "isotropic" correlation length into two distinct scales which characterize the longitudinal and transverse order in string fluids and, hence, may be used as a fingerprint of this transition. The comparison of the proposed OZ theory with the Monte Carlo simulations reveals fairly good agreement.
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We propose a simple method to approximately predict the freezing (fluid-solid) phase transition in systems of particles interacting via purely repulsive potentials. The method is based on the striking universality of the freezing curve for the model Yukawa and inverse-power-law interactions. This method is applied to draw an exemplary phase diagram of complex plasmas. We suggest that it can also be used to locate freezing transition in other substances with similar properties of interaction.
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Spectra of phonons with out-of-plane polarization were studied experimentally in a 2D plasma crystal. The dispersion relation was directly measured for the first time using a novel method of particle imaging. The out-of-plane mode was proven to have negative optical dispersion at small wave numbers, comparison with theory showed good agreement. The effect of the plasma wakes on the dispersion relation is briefly discussed.
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Along Ulysses' path from Jupiter to the south ecliptic pole, the onboard dust detector measured a dust impact rate that varied slowly from 0.2 to 0.5 impacts per day. The dominant component of the dust flux arrived from an ecliptic latitude and longitude of 100 + 10 degrees and 280 degrees +/- 30 degrees which indicates an interstellar origin. An additional flux of small particles, which do not come from the interstellar direction and are unlikely to be zodiacal dust grains, appeared south of -45 degrees latitude. One explanation is that these particles are beta-meteoroids accelerated away from the sun by radiation pressure and electromagnetic forces.