RESUMO
Cases of canine onchocerciasis caused by Onchocerca lupi are increasingly reported from Europe and the western United States of America. The zoonotic role of this parasite had already been suspected in Europe as the clinical signs and histopathology seen in two ocular cases from Albania and the Crimean region were very similar to those of canine ocular onchocerciasis. In the most recent reports of human onchocerciasis, O. lupi has been morphologically and molecularly identified as the causative agent of ocular infestation in two patients from Turkey, and one patient from Tunisia. Here, we report an additional case of nodular lesions involving two, and possibly more, immature worms in a patient from Iran. The parasite was found to belong to the genus Onchocerca based on morphological features and the species was confirmed as O. lupi from a partial sequence analysis of 12S ribosomal DNA.
Assuntos
Onchocerca/isolamento & purificação , Oncocercose Ocular/diagnóstico , Oncocercose Ocular/patologia , Animais , Análise por Conglomerados , DNA de Helmintos/química , DNA de Helmintos/genética , DNA Ribossômico/química , DNA Ribossômico/genética , DNA Espaçador Ribossômico/química , DNA Espaçador Ribossômico/genética , Humanos , Irã (Geográfico) , Masculino , Dados de Sequência Molecular , Oncocercose Ocular/parasitologia , Filogenia , RNA Ribossômico/genética , Análise de Sequência de DNA , Adulto JovemRESUMO
Parasite strain characterization is essential for the establishment of a prevention and control strategy in any endemic area. The aim of this study was to characterize different Echinococcus granulosus isolates from Iran by using DNA sequences of the mitochondrial 12S rRNA gene. Thirty livers and lungs of cattle, sheep and goats naturally infected with E. granulosus were collected from abattoirs in northern and western Iran between June and October 2007. These samples yielded 18 fertile cysts which we used for the genetic work. We designed and tested two new primer pairs which specifically amplify portions of the mitochondrial 12S rRNA gene of the two strains (G1 and G6) of E. granulosus known to occur in Iran. One primer pair amplified a fragment of 259 base pairs (bp) from only the G1 strain. The second pair amplified a fragment of 676 bp from the G6 strain. The G1 genotype was identified in all fertile cyst samples, in agreement with previous studies in Iran. Ten of our samples and a single reference sample of the G6 strain were sequenced and compared with the G1 and G6 sequences deposited in GenBank.
Assuntos
Echinococcus granulosus/classificação , Genes de Helmintos , RNA Ribossômico/análise , Animais , Sequência de Bases , Bovinos , Doenças dos Bovinos/parasitologia , Equinococose/parasitologia , Equinococose/veterinária , Echinococcus granulosus/genética , Genótipo , Doenças das Cabras/parasitologia , Cabras , Enteropatias Parasitárias/parasitologia , Enteropatias Parasitárias/veterinária , Irã (Geográfico) , Dados de Sequência Molecular , Parasitologia/métodos , RNA , Ovinos , Doenças dos Ovinos/parasitologiaRESUMO
The proventriculi of 11 Eurasian cranes (Grus grus) from central Iran were examined for the existence of parasitic helminths. Preliminary reports suggested that the death of these birds was related to untimely cold weather. Nine proventriculi (82%) were heavily infected by the nematode Tetrameres grusi. Glandular structure of the infected proventriculi was replaced by epithelial atrophy but significant inflammatory reactions were not observed in any of the infected organs. In serious infections, the nematode produced vast structural and functional changes, causing organ dysfunction and glandular necrosis. The coincidence of heavy helminth infection at times of environmental stress may lead to debilitation, wasting, and perhaps mortality in migratory cranes.
Assuntos
Doenças das Aves/epidemiologia , Infecções por Spirurida/veterinária , Spiruroidea/isolamento & purificação , Animais , Doenças das Aves/mortalidade , Doenças das Aves/patologia , Aves , Conservação dos Recursos Naturais , Feminino , Irã (Geográfico)/epidemiologia , Masculino , Proventrículo/parasitologia , Infecções por Spirurida/epidemiologia , Infecções por Spirurida/mortalidade , Infecções por Spirurida/patologiaRESUMO
A total of 12 gastrointestinal tracts of wild boars (Sus scrofa) from western Iran (Luristan) were examined for protozoan infection between September 2000 and November 2001. Of 12 boars examined, 67% harbored one or more species of the following protozoa: Balantidium coli (25%), Tritrichomonas suis (25%), Blastocystis sp. (25%), Entamoeba polecki (17%), Entamoeba suis (8%), Iodamoeba butschlii (17%), and Chilomastix mesnili (8%). Four of these protozoan species also are reported in humans, and persons living in rural areas where wild boars are abundant should take precaution to avoid infection.
Assuntos
Trato Gastrointestinal/parasitologia , Infecções Protozoárias em Animais/epidemiologia , Sus scrofa/parasitologia , Doenças dos Suínos/epidemiologia , Animais , Reservatórios de Doenças/veterinária , Feminino , Irã (Geográfico)/epidemiologia , Masculino , Infecções Protozoárias em Animais/parasitologia , Saúde Pública , Doenças dos Suínos/parasitologiaRESUMO
Polycephalic larvae of Taenia endothoracicus were found from naturally infected gerbils Meriones lybicus in a rural area of Arak, central Iran. A large cyst containing 19 protoscolices was located in the peritoneum, attached to the large omentum. The characteristics of the protoscolices and rostellar hooks confirmed the identification of these larvae as T. endothoracicus.