RESUMO
Apeu virus (APEUV) (family Bunyaviridae, genus Orthobunyavirus) was plaque purified and characterised by serological and molecular analysis. Neutralising assays confirmed cross-reactivity between purified APEUV clones and the Caraparu virus complex of group C orthobunyaviruses. Partial sequencing of the L, M and S segments of one APEUV clone (APEUV-CL5) was carried out. A phylogenetic tree constructed with the L amino acid sequences clustered APEUV-CL5 within the genus Orthobunyavirus, confirming its serological classification. Analysis of M segment sequences clustered APEUV-CL5 in the Caraparu virus complex (Group C), in agreement with serological tests and previous molecular characterisation. However, the sequence of the nucleocapsid gene (N) gave low identity values when compared to those of the group C viruses. The phylogenetic tree based on N nucleotide sequences clustered APEUV-CL5 next to the California and Bwamba groups. This remarkable S nucleotide variability suggests that APEUV-CL5 could be a genetic reassortant and that this evolutionary mechanism is present in the history of the group C viruses.
Assuntos
Infecções por Bunyaviridae/virologia , Orthobunyavirus/classificação , Orthobunyavirus/isolamento & purificação , Filogenia , Animais , Anticorpos Antivirais/imunologia , Linhagem Celular , Humanos , Camundongos , Dados de Sequência Molecular , Orthobunyavirus/genética , Orthobunyavirus/imunologiaRESUMO
The Tahyna virus (TAHV) is an important human pathogen in the Bunyaviridae family. To date, only the S and M segments of this virus have been sequenced, but the sequence of the L segment hasn't been established yet. In this study, we sequenced 963 nucleotides of the L segment of TAHV, comprising pre-motif A and motif A in region 3 of the RNA polymerase gene.
Assuntos
RNA Polimerases Dirigidas por DNA/genética , Vírus da Encefalite da Califórnia/classificação , Vírus da Encefalite da Califórnia/genética , Genoma Viral , Filogenia , Proteínas Virais/genética , Motivos de Aminoácidos , Sequência de Aminoácidos , RNA Polimerases Dirigidas por DNA/química , Humanos , Dados de Sequência Molecular , Orthobunyavirus/classificação , Orthobunyavirus/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido Nucleico , Proteínas Virais/químicaRESUMO
Here, for the first time, we report the nucleotide sequence of Caraparu virus (CARV) L segment and the analysis of the RNA polymerase region 3 encoded by this segment. The 1,404 bp nucleotide sequence shares the highest identity with Bunyamwera, La Crosse, Oropouche, and Akabane virus sequences. The amino acid sequence was deduced and aligned with sequences from members of the Bunyaviridae family and used for phylogenetic analysis. The CARV clustered in the Orthobunyavirus genus. The premotif A and motifs A-E are present in the region 3 of the Bunyaviridae family, were also conserved in CARV L protein, as well as other conserved regions among Orthobunyavirus genus.