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1.
PLoS Comput Biol ; 20(5): e1012085, 2024 May.
Artigo em Inglês | MEDLINE | ID: mdl-38709845

RESUMO

Alzheimer's Disease (AD) is characterized by a range of behavioral alterations, including memory loss and psychiatric symptoms. While there is evidence that molecular pathologies, such as amyloid beta (Aß), contribute to AD, it remains unclear how this histopathology gives rise to such disparate behavioral deficits. One hypothesis is that Aß exerts differential effects on neuronal circuits across brain regions, depending on the neurophysiology and connectivity of different areas. To test this, we recorded from large neuronal populations in dorsal CA1 (dCA1) and ventral CA1 (vCA1), two hippocampal areas known to be structurally and functionally diverse, in the APP/PS1 mouse model of amyloidosis. Despite similar levels of Aß pathology, dCA1 and vCA1 showed distinct disruptions in neuronal population activity as animals navigated a virtual reality environment. In dCA1, pairwise correlations and entropy, a measure of the diversity of activity patterns, were decreased in APP/PS1 mice relative to age-matched C57BL/6 controls. However, in vCA1, APP/PS1 mice had increased pair-wise correlations and entropy as compared to age matched controls. Finally, using maximum entropy models, we connected the microscopic features of population activity (correlations) to the macroscopic features of the population code (entropy). We found that the models' performance increased in predicting dCA1 activity, but decreased in predicting vCA1 activity, in APP/PS1 mice relative to the controls. Taken together, we found that Aß exerts distinct effects across different hippocampal regions, suggesting that the various behavioral deficits of AD may reflect underlying heterogeneities in neuronal circuits and the different disruptions that Aß pathology causes in those circuits.


Assuntos
Doença de Alzheimer , Precursor de Proteína beta-Amiloide , Região CA1 Hipocampal , Animais , Masculino , Camundongos , Doença de Alzheimer/metabolismo , Doença de Alzheimer/fisiopatologia , Doença de Alzheimer/patologia , Doença de Alzheimer/genética , Peptídeos beta-Amiloides/metabolismo , Precursor de Proteína beta-Amiloide/genética , Precursor de Proteína beta-Amiloide/metabolismo , Região CA1 Hipocampal/metabolismo , Região CA1 Hipocampal/fisiopatologia , Região CA1 Hipocampal/patologia , Biologia Computacional , Modelos Animais de Doenças , Camundongos Endogâmicos C57BL , Camundongos Transgênicos , Neurônios/metabolismo , Neurônios/patologia , Presenilina-1/genética , Presenilina-1/metabolismo
2.
Eur J Neurosci ; 56(9): 5564-5586, 2022 11.
Artigo em Inglês | MEDLINE | ID: mdl-35244297

RESUMO

Alzheimer's disease (AD) is a neurodegenerative disorder characterized by behavioural changes that include memory loss and cognitive decline and is associated with the appearance of amyloid-ß plaques and neurofibrillary tangles throughout the brain. Although aspects of the disease percolate across multiple levels of neuronal organization, from the cellular to the behavioural, it is increasingly clear that circuits are a critical junction between the cellular pathology and the behavioural phenotypes that bookend these levels of analyses. In this review, we discuss critical aspects of neural circuit research, beginning with synapses and progressing to network activity and how they influence our understanding of disease processed in AD.


Assuntos
Doença de Alzheimer , Animais , Doença de Alzheimer/genética , Emaranhados Neurofibrilares/patologia , Placa Amiloide , Peptídeos beta-Amiloides , Sinapses/fisiologia , Modelos Animais de Doenças
3.
J Neurophysiol ; 125(5): 1612-1623, 2021 05 01.
Artigo em Inglês | MEDLINE | ID: mdl-33656931

RESUMO

Neural codes for sensory inputs have been hypothesized to reside in a broader space defined by ongoing patterns of spontaneous activity. To understand the structure of this spontaneous activity in the olfactory system, we performed high-density recordings of neural populations in the main olfactory bulb of awake mice. We observed changes in pairwise correlations of spontaneous activity between mitral and tufted (M/T) cells when animals were running, which resulted in an increase in the entropy of the population. Surprisingly, pairwise maximum entropy models that described the population activity using only assumptions about the firing rates and correlations of neurons were better at predicting the global structure of activity when animals were stationary as compared to when they were running, implying that higher order (3rd, 4th order) interactions governed population activity during locomotion. Taken together, we found that locomotion alters the functional interactions that shape spontaneous population activity at the earliest stages of olfactory processing, one synapse away from the sensory receptors in the nasal epithelium. These data suggest that the coding space available for sensory representations responds adaptively to the animal's behavioral state.NEW & NOTEWORTHY The organization and structure of spontaneous population activity in the olfactory system places constraints of how odor information is represented. Using high-density electrophysiological recordings of mitral and tufted cells, we found that running increases the dimensionality of spontaneous activity, implicating higher order interactions among neurons during locomotion. Behavior, thus, flexibly alters neuronal activity at the earliest stages of sensory processing.


Assuntos
Comportamento Animal/fisiologia , Rede Nervosa/fisiologia , Bulbo Olfatório/fisiologia , Percepção Olfatória/fisiologia , Corrida/fisiologia , Animais , Fenômenos Eletrofisiológicos/fisiologia , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL
4.
Eur J Neurosci ; 51(3): 721-730, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31849113

RESUMO

Sex differences in running behaviors between female and male mice occur naturally in the wild. Recent experiments using head-fixed mice on a voluntary running wheel have exploited analogous locomotor activity to gain insight into the neural underpinnings of a number of behaviors ranging from spatial navigation to decision-making. It is however largely unknown if sex differences exist between females and males in a head-fixed experimental paradigm. To address this, we characterized locomotor activity in head-fixed female and male C57BL/6J mice on a voluntary running wheel. First, we found that over the initial 7-day period, on average, animals increased both the velocity and the time spent running. Furthermore, we found that female mice habituated to running forward over the initial 2 days of encountering the wheel, while male mice took up to 4 days to habituate to running forward. Taken together, we characterized features of a sexually divergent behavior in head-fixed running that should be considered in experiments employing female and male mice.


Assuntos
Atividade Motora , Caracteres Sexuais , Animais , Feminino , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Tempo
5.
Nature ; 502(7471): 327-332, 2013 Oct 17.
Artigo em Inglês | MEDLINE | ID: mdl-24107995

RESUMO

Progressive phases of multiple sclerosis are associated with inhibited differentiation of the progenitor cell population that generates the mature oligodendrocytes required for remyelination and disease remission. To identify selective inducers of oligodendrocyte differentiation, we performed an image-based screen for myelin basic protein (MBP) expression using primary rat optic-nerve-derived progenitor cells. Here we show that among the most effective compounds identifed was benztropine, which significantly decreases clinical severity in the experimental autoimmune encephalomyelitis (EAE) model of relapsing-remitting multiple sclerosis when administered alone or in combination with approved immunosuppressive treatments for multiple sclerosis. Evidence from a cuprizone-induced model of demyelination, in vitro and in vivo T-cell assays and EAE adoptive transfer experiments indicated that the observed efficacy of this drug results directly from an enhancement of remyelination rather than immune suppression. Pharmacological studies indicate that benztropine functions by a mechanism that involves direct antagonism of M1 and/or M3 muscarinic receptors. These studies should facilitate the development of effective new therapies for the treatment of multiple sclerosis that complement established immunosuppressive approaches.


Assuntos
Benzotropina/uso terapêutico , Encefalomielite Autoimune Experimental/tratamento farmacológico , Modelos Biológicos , Esclerose Múltipla/tratamento farmacológico , Bainha de Mielina/efeitos dos fármacos , Oligodendroglia/efeitos dos fármacos , Regeneração/efeitos dos fármacos , Animais , Antiparkinsonianos/farmacologia , Antiparkinsonianos/uso terapêutico , Benzotropina/farmacologia , Diferenciação Celular/efeitos dos fármacos , Técnicas de Cocultura , Cuprizona/farmacologia , Cuprizona/uso terapêutico , Encefalomielite Autoimune Experimental/induzido quimicamente , Encefalomielite Autoimune Experimental/patologia , Feminino , Cloridrato de Fingolimode , Sistema Imunitário/efeitos dos fármacos , Sistema Imunitário/imunologia , Camundongos , Camundongos Endogâmicos C57BL , Esclerose Múltipla/patologia , Proteína Proteolipídica de Mielina/farmacologia , Bainha de Mielina/metabolismo , Bainha de Mielina/patologia , Oligodendroglia/citologia , Oligodendroglia/metabolismo , Oligodendroglia/patologia , Nervo Óptico/citologia , Propilenoglicóis/farmacologia , Propilenoglicóis/uso terapêutico , Ratos , Receptor Muscarínico M1/antagonistas & inibidores , Receptor Muscarínico M1/metabolismo , Receptor Muscarínico M3/antagonistas & inibidores , Receptor Muscarínico M3/metabolismo , Recidiva , Esfingosina/análogos & derivados , Esfingosina/farmacologia , Esfingosina/uso terapêutico , Células-Tronco/citologia , Células-Tronco/efeitos dos fármacos
6.
Development ; 142(12): 2213-25, 2015 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-26015546

RESUMO

Myelination in the central nervous system is the process by which oligodendrocytes form myelin sheaths around the axons of neurons. Myelination enables neurons to transmit information more quickly and more efficiently and allows for more complex brain functions; yet, remarkably, the underlying mechanism by which myelination occurs is still not fully understood. A reliable in vitro assay is essential to dissect oligodendrocyte and myelin biology. Hence, we developed a protocol to generate myelinating oligodendrocytes from mouse embryonic stem cells and established a myelin formation assay with embryonic stem cell-derived neurons in microfluidic devices. Myelin formation was quantified using a custom semi-automated method that is suitable for larger scale analysis. Finally, early myelination was followed in real time over several days and the results have led us to propose a new model for myelin formation.


Assuntos
Sistema Nervoso Central/embriologia , Células-Tronco Embrionárias/citologia , Bainha de Mielina/metabolismo , Oligodendroglia/metabolismo , Animais , Axônios/metabolismo , Células Cultivadas , Técnicas de Cocultura , Células-Tronco Pluripotentes Induzidas/citologia , Camundongos , Técnicas Analíticas Microfluídicas , Neurônios/metabolismo , Receptores de Detecção de Cálcio , Receptores Acoplados a Proteínas G/metabolismo
7.
Mol Psychiatry ; 22(6): 820-835, 2017 06.
Artigo em Inglês | MEDLINE | ID: mdl-27378147

RESUMO

Autism spectrum disorders (ASD) are common, complex and heterogeneous neurodevelopmental disorders. Cellular and molecular mechanisms responsible for ASD pathogenesis have been proposed based on genetic studies, brain pathology and imaging, but a major impediment to testing ASD hypotheses is the lack of human cell models. Here, we reprogrammed fibroblasts to generate induced pluripotent stem cells, neural progenitor cells (NPCs) and neurons from ASD individuals with early brain overgrowth and non-ASD controls with normal brain size. ASD-derived NPCs display increased cell proliferation because of dysregulation of a ß-catenin/BRN2 transcriptional cascade. ASD-derived neurons display abnormal neurogenesis and reduced synaptogenesis leading to functional defects in neuronal networks. Interestingly, defects in neuronal networks could be rescued by insulin growth factor 1 (IGF-1), a drug that is currently in clinical trials for ASD. This work demonstrates that selection of ASD subjects based on endophenotypes unraveled biologically relevant pathway disruption and revealed a potential cellular mechanism for the therapeutic effect of IGF-1.


Assuntos
Transtorno Autístico/metabolismo , Transtorno Autístico/patologia , Técnicas de Cultura de Tecidos/métodos , Adolescente , Transtorno do Espectro Autista/metabolismo , Transtorno do Espectro Autista/fisiopatologia , Encéfalo/metabolismo , Proliferação de Células/genética , Células Cultivadas , Criança , Pré-Escolar , Feminino , Fibroblastos/metabolismo , Humanos , Células-Tronco Pluripotentes Induzidas/metabolismo , Fator de Crescimento Insulin-Like I/metabolismo , Fator de Crescimento Insulin-Like I/uso terapêutico , Masculino , Células-Tronco Neurais/metabolismo , Neurogênese , Neurônios/metabolismo , Neurônios/fisiologia , beta Catenina/metabolismo
8.
J Neurosci ; 36(28): 7535-45, 2016 07 13.
Artigo em Inglês | MEDLINE | ID: mdl-27413162

RESUMO

UNLABELLED: Although feedback or centrifugal projections from higher processing centers of the brain to peripheral regions have long been known to play essential functional roles, the anatomical organization of these connections remains largely unknown. Using a virus-based retrograde labeling strategy and 3D whole-brain reconstruction methods, we mapped the spatial organization of centrifugal projections from two olfactory cortical areas, the anterior olfactory nucleus (AON) and the piriform cortex, to the granule cell layer of the main olfactory bulb in the mouse. Both regions are major recipients of information from the bulb and are the largest sources of feedback to the bulb, collectively constituting circuits essential for olfactory coding and olfactory behavior. We found that, although ipsilateral inputs from the AON were uniformly distributed, feedback from the contralateral AON had a strong ventral bias. In addition, we observed that centrifugally projecting neurons were spatially clustered in the piriform cortex, in contrast to the distributed feedforward axonal inputs that these cells receive from the principal neurons of the bulb. Therefore, information carried from the bulb to higher processing structures by anatomically stereotypic projections is likely relayed back to the bulb by organizationally distinct feedback projections that may reflect different coding strategies and therefore different functional roles. SIGNIFICANCE STATEMENT: Principles of anatomical organization, sometimes instantiated as "maps" in the mammalian brain, have provided key insights into the structure and function of circuits in sensory systems. Generally, these characterizations focus on projections from early sensory processing areas to higher processing structures despite considerable evidence that feedback or centrifugal projections often constitute major conduits of information flow. Our results identify structure in the organization of centrifugal feedback projections to the olfactory bulb that is fundamentally different from the organization of feedforward circuits. Our study suggests that understanding computations performed in the olfactory bulb, and more generally in the olfactory system, requires understanding interactions between feedforward and feedback "maps" both structurally and functionally.


Assuntos
Mapeamento Encefálico , Bulbo Olfatório/citologia , Córtex Olfatório/fisiologia , Condutos Olfatórios/fisiologia , Células Receptoras Sensoriais/fisiologia , Olfato , Animais , Análise por Conglomerados , Lateralidade Funcional , Glicoproteínas/metabolismo , Imageamento Tridimensional , Proteínas Luminescentes/genética , Proteínas Luminescentes/metabolismo , Camundongos , Camundongos Endogâmicos C57BL , Bulbo Olfatório/diagnóstico por imagem , Córtex Olfatório/diagnóstico por imagem , Condutos Olfatórios/diagnóstico por imagem , Transdução Genética
9.
Proc Natl Acad Sci U S A ; 110(20): 8248-53, 2013 May 14.
Artigo em Inglês | MEDLINE | ID: mdl-23630284

RESUMO

Cell-to-cell variability in molecular, genetic, and physiological features is increasingly recognized as a critical feature of complex biological systems, including the brain. Although such variability has potential advantages in robustness and reliability, how and why biological circuits assemble heterogeneous cells into functional groups is poorly understood. Here, we develop analytic approaches toward answering how neuron-level variation in intrinsic biophysical properties of olfactory bulb mitral cells influences population coding of fluctuating stimuli. We capture the intrinsic diversity of recorded populations of neurons through a statistical approach based on generalized linear models. These models are flexible enough to predict the diverse responses of individual neurons yet provide a common reference frame for comparing one neuron to the next. We then use Bayesian stimulus decoding to ask how effectively different populations of mitral cells, varying in their diversity, encode a common stimulus. We show that a key advantage provided by physiological levels of intrinsic diversity is more efficient and more robust encoding of stimuli by the population as a whole. However, we find that the populations that best encode stimulus features are not simply the most heterogeneous, but those that balance diversity with the benefits of neural similarity.


Assuntos
Modelos Neurológicos , Neurônios/fisiologia , Potenciais de Ação/fisiologia , Algoritmos , Animais , Teorema de Bayes , Eletrofisiologia , Canais Iônicos/metabolismo , Modelos Lineares , Camundongos , Neurônios/metabolismo , Bulbo Olfatório/patologia , Reprodutibilidade dos Testes
10.
Neural Comput ; 27(8): 1609-23, 2015 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-26079749

RESUMO

Understanding a neuron's transfer function, which relates a neuron's inputs to its outputs, is essential for understanding the computational role of single neurons. Recently, statistical models, based on point processes and using generalized linear model (GLM) technology, have been widely applied to predict dynamic neuronal transfer functions. However, the standard version of these models fails to capture important features of neural activity, such as responses to stimuli that elicit highly reliable trial-to-trial spiking. Here, we consider a generalization of the usual GLM that incorporates nonlinearity by modeling reliable and nonreliable spikes as being generated by distinct stimulus features. We develop and apply these models to spike trains from olfactory bulb mitral cells recorded in vitro. We find that spike generation in these neurons is better modeled when reliable and unreliable spikes are considered separately and that this effect is most pronounced for neurons with a large number of both reliable and unreliable spikes.


Assuntos
Potenciais de Ação/fisiologia , Modelos Neurológicos , Neurônios/fisiologia , Animais , Fenômenos Biofísicos , Simulação por Computador , Modelos Lineares , Dinâmica não Linear , Bulbo Olfatório/citologia , Fatores de Tempo
11.
J Neurophysiol ; 112(5): 1054-66, 2014 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-24899672

RESUMO

Recent interest has emerged on the role of intrinsic biophysical diversity in neuronal coding. An important question in neurophysiology is understanding which voltage-gated ion channels are responsible for this diversity and how variable expression or activity of one class of ion channels across neurons of a single type affects they way populations carry information. In mitral cells in the olfactory bulb of mice, we found that biophysical diversity was conferred in part by 4-aminopyridine (4-AP)-sensitive potassium channels and reduced following block of those channels. When populations of mitral cells were stimulated with identical inputs, the diversity exhibited in their output spike patterns reduced with the addition of 4-AP, decreasing the stimulus information carried by ensembles of 15 neurons from 437 ± 15 to 397 ± 19 bits/s. Decreases in information were due to reduction in the diversity of population spike patterns generated in response to different features of the stimulus, suggesting that the coding capacity of a population can be altered by changes in the function of single ion channel types.


Assuntos
4-Aminopiridina/farmacologia , Potenciais de Ação/fisiologia , Neurônios/fisiologia , Bulbo Olfatório/fisiologia , Bloqueadores dos Canais de Potássio/farmacologia , Canais de Potássio de Abertura Dependente da Tensão da Membrana/fisiologia , Potenciais de Ação/efeitos dos fármacos , Animais , Feminino , Teoria da Informação , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Neurônios/efeitos dos fármacos , Bulbo Olfatório/efeitos dos fármacos
12.
bioRxiv ; 2024 Jul 04.
Artigo em Inglês | MEDLINE | ID: mdl-39005290

RESUMO

In the early olfactory system, adult-neurogenesis, a process of neuronal replacement results in the continuous reorganization of synaptic connections and network architecture throughout the animal's life. This poses a critical challenge: How does the olfactory system maintain stable representations of odors and therefore allow for stable sensory perceptions amidst this ongoing circuit instability? Utilizing a detailed spiking network model of early olfactory circuits, we uncovered dual roles for adult-neurogenesis: one that both supports representational stability to faithfully encode odor information and also one that facilitates plasticity to allow for learning and adaptation. In the main olfactory bulb, adult-neurogenesis affects neural codes in individual mitral and tufted cells but preserves odor representations at the neuronal population level. By contrast, in the olfactory piriform cortex, both individual cell responses and overall population dynamics undergo progressive changes due to adult-neurogenesis. This leads to representational drift, a gradual alteration in sensory perception. Both processes are dynamic and depend on experience such that repeated exposure to specific odors reduces the drift due to adult-neurogenesis; thus, when the odor environment is stable over the course of adult-neurogenesis, it is neurogenesis that actually allows the representations to remain stable in piriform cortex; when those olfactory environments change, adult-neurogenesis allows the cortical representations to track environmental change. Whereas perceptual stability and plasticity due to learning are often thought of as two distinct, often contradictory processing in neuronal coding, we find that adult-neurogenesis serves as a shared mechanism for both. In this regard, the quixotic presence of adult-neurogenesis in the mammalian olfactory bulb that has been the focus of considerable debate in chemosensory neuroscience may be the mechanistic underpinning behind an array of complex computations.

13.
Stem Cell Reports ; 19(6): 796-816, 2024 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-38759644

RESUMO

Human brain organoid models have emerged as a promising tool for studying human brain development and function. These models preserve human genetics and recapitulate some aspects of human brain development, while facilitating manipulation in an in vitro setting. Despite their potential to transform biology and medicine, concerns persist about their fidelity. To fully harness their potential, it is imperative to establish reliable analytic methods, ensuring rigor and reproducibility. Here, we review current analytical platforms used to characterize human forebrain cortical organoids, highlight challenges, and propose recommendations for future studies to achieve greater precision and uniformity across laboratories.


Assuntos
Encéfalo , Organoides , Humanos , Organoides/citologia , Organoides/metabolismo , Encéfalo/citologia , Reprodutibilidade dos Testes , Prosencéfalo/citologia
14.
bioRxiv ; 2023 Nov 02.
Artigo em Inglês | MEDLINE | ID: mdl-37961249

RESUMO

Multi-electrode recording of neural activity in cultures offer opportunities for understanding how the structure of a network gives rise to function. Although it is hypothesized that network size is critical for determining the dynamics of activity, this relationship in human neural cultures remains largely unexplored. By applying new methods for analyzing neural activity to human iPSC derived cultures at either low-densities or high-densities, we uncovered the significant impacts that neuron number has on the individual neurophysiological properties of cells (such as firing rates), the collective behavior of the networks these cultures formed (as measured by entropy), and the relationship between the two. As a result, simply changing the densities of neurons generated dynamics and network behavior that differed not just in degree, but in kind. Beyond revealing the relationship between network structure and function, our findings provide a novel analytical framework to study diseases where network level activity is affected.

15.
Phys Rev E ; 108(5-1): 054404, 2023 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-38115483

RESUMO

Although temporal coding through spike-time patterns has long been of interest in neuroscience, the specific structures that could be useful for spike-time codes remain highly unclear. Here, we introduce an analytical approach, using techniques from discrete mathematics, to study spike-time codes. As an initial example, we focus on the phenomenon of "phase precession" in the rodent hippocampus. During navigation and learning on a physical track, specific cells in a rodent's brain form a highly structured pattern relative to the oscillation of population activity in this region. Studies of phase precession largely focus on its role in precisely ordering spike times for synaptic plasticity, as the role of phase precession in memory formation is well established. Comparatively less attention has been paid to the fact that phase precession represents one of the best candidates for a spike-time neural code. The precise nature of this code remains an open question. Here, we derive an analytical expression for a function mapping points in physical space to complex-valued spikes by representing individual spike times as complex numbers. The properties of this function make explicit a specific relationship between past and future in spike patterns of the hippocampus. Importantly, this mathematical approach generalizes beyond the specific phenomenon studied here, providing a technique to study the neural codes within precise spike-time sequences found during sensory coding and motor behavior. We then introduce a spike-based decoding algorithm, based on this function, that successfully decodes a simulated animal's trajectory using only the animal's initial position and a pattern of spike times. This decoder is robust to noise in spike times and works on a timescale almost an order of magnitude shorter than typically used with decoders that work on average firing rate. These results illustrate the utility of a discrete approach, based on the structure and symmetries in spike patterns across finite sets of cells, to provide insight into the structure and function of neural systems.


Assuntos
Encéfalo , Hipocampo , Animais , Potenciais de Ação , Algoritmos , Modelos Neurológicos
16.
Commun Biol ; 6(1): 572, 2023 05 29.
Artigo em Inglês | MEDLINE | ID: mdl-37248385

RESUMO

The laboratory mouse has provided tremendous insight to the underpinnings of mammalian central nervous system physiology. In recent years, it has become possible to image single neurons, glia and vascular cells in vivo by using head-fixed preparations combined with cranial windows to study local networks of activity in the living brain. Such approaches have also succeeded without the use of general anesthesia providing insights to the natural behaviors of the central nervous system. However, the same has not yet been developed for the eye, which is constantly in motion. Here we characterize a novel head-fixed preparation that enables high-resolution adaptive optics retinal imaging at the single-cell level in awake-behaving mice. We reveal three new functional attributes of the normal eye that are overlooked by anesthesia: 1) High-frequency, low-amplitude eye motion of the mouse that is only present in the awake state 2) Single-cell blood flow in the mouse retina is reduced under anesthesia and 3) Mouse retinae thicken in response to ketamine/xylazine anesthesia. Here we show key benefits of the awake-behaving preparation that enables study of retinal physiology without anesthesia to study the normal retinal physiology in the mouse.


Assuntos
Ketamina , Vigília , Camundongos , Animais , Vigília/fisiologia , Retina/diagnóstico por imagem , Retina/fisiologia , Ketamina/farmacologia , Diagnóstico por Imagem , Xilazina/farmacologia , Mamíferos
17.
Cell Rep ; 38(12): 110545, 2022 03 22.
Artigo em Inglês | MEDLINE | ID: mdl-35320723

RESUMO

In chemical sensation, multiple models have been proposed to explain how odors are represented in the olfactory cortex. One hypothesis is that the combinatorial identity of active neurons within sniff-related time windows is critical, whereas another model proposes that it is the temporal structure of neural activity that is essential for encoding odor information. We find that top-down feedback to the main olfactory bulb dictates the information transmitted to the piriform cortex and switches between these coding strategies. Using a detailed network model, we demonstrate that feedback control of inhibition influences the excitation-inhibition balance in mitral cells, restructuring the dynamics of piriform cortical cells. This results in performance improvement in odor discrimination tasks. These findings present a framework for early olfactory computation, where top-down feedback to the bulb flexibly shapes the temporal structure of neural activity in the piriform cortex, allowing the early olfactory system to dynamically switch between two distinct coding models.


Assuntos
Córtex Olfatório , Córtex Piriforme , Retroalimentação , Bulbo Olfatório , Córtex Olfatório/fisiologia , Córtex Piriforme/fisiologia , Olfato/fisiologia
18.
eNeuro ; 8(5)2021.
Artigo em Inglês | MEDLINE | ID: mdl-34433573

RESUMO

Molecular, anatomic, and behavioral studies show that the hippocampus is structurally and functionally heterogeneous, with dorsal hippocampus implicated in mnemonic processes and spatial navigation and ventral hippocampus involved in affective processes. By performing electrophysiological recordings of large neuronal populations in dorsal and ventral CA1 in head-fixed mice navigating a virtual environment, we found that this diversity resulted in different strategies for population coding of space. Populations of neurons in dorsal CA1 showed more complex patterns of activity, which resulted in a higher dimensionality of neural representations that translated to more information being encoded, as compared ensembles in vCA1. Furthermore, a pairwise maximum entropy model was better at predicting the structure of these global patterns of activity in ventral CA1 as compared with dorsal CA1. Taken together, the different coding strategies we uncovered likely emerge from anatomic and physiological differences along the longitudinal axis of hippocampus and that may, in turn, underpin the divergent ethological roles of dorsal and ventral CA1.


Assuntos
Hipocampo , Neurônios , Animais , Região CA1 Hipocampal , Camundongos
19.
Front Comput Neurosci ; 14: 59, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32765248

RESUMO

Growing evidence shows that top-down projections from excitatory neurons in piriform cortex selectively synapse onto local inhibitory granule cells in the main olfactory bulb, effectively gating their own inputs by controlling inhibition. An open question in olfaction is the role this feedback plays in shaping the dynamics of local circuits, and the resultant computational benefits it provides. Using rate models of neuronal firing in a network consisting of excitatory mitral and tufted cells, inhibitory granule cells and top-down piriform cortical neurons, we found that changes in the weight of feedback to inhibitory neurons generated diverse network dynamics and complex transitions between these dynamics. Changes in the weight of top-down feedback supported a number of computations, including both pattern separation and oscillatory synchrony. Additionally, the network could generate gamma oscillations though a mechanism we termed Top-down control of Inhibitory Neuron Gamma (TING). Collectively, these functions arose from a codimension-2 bifurcation in the dynamical system. Our results highlight a key role for this top-down feedback, gating inhibition to facilitate often diametrically different computations.

20.
Sci Rep ; 10(1): 1077, 2020 01 23.
Artigo em Inglês | MEDLINE | ID: mdl-31974405

RESUMO

While the link between amyloid ß (Aß) accumulation and synaptic degradation in Alzheimer's disease (AD) is known, the consequences of this pathology on population coding remain unknown. We found that the entropy, a measure of the diversity of network firing patterns, was lower in the dorsal CA1 region in the APP/PS1 mouse model of Aß pathology, relative to controls, thereby reducing the population's coding capacity. Our results reveal a network level signature of the deficits Aß accumulation causes to the computations performed by neural circuits.


Assuntos
Doença de Alzheimer/metabolismo , Precursor de Proteína beta-Amiloide/metabolismo , Região CA1 Hipocampal/metabolismo , Neurônios/citologia , Doença de Alzheimer/genética , Doença de Alzheimer/patologia , Precursor de Proteína beta-Amiloide/genética , Animais , Região CA1 Hipocampal/patologia , Modelos Animais de Doenças , Feminino , Humanos , Masculino , Camundongos , Neurônios/metabolismo , Presenilina-1/genética , Presenilina-1/metabolismo
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