Role of Hyperplasia of Gingival Lymphatics in Periodontal Inflammation.

Lymphatic vessels are important for maintenance of tissue fluid homeostasis and afferent antigen transport. In chronic inflammation, lymphangiogenesis takes place and is characterized by lymphatic endothelial cell proliferation and lymphatic hyperplasia. Vascular endothelial growth factor C (VEGFC) is the main known lymphangiogenic growth factor, and its expression is increased in periodontitis, a common chronic infectious disease that results in tissue destruction and alveolar bone loss. The role of lymphangiogenesis during development of periodontitis is unknown. Here, we test if transgenic overexpression of epithelial VEGFC in a murine model is followed by hyperplasia of lymphatic vessels in oral mucosa and if the lymphatic drainage capacity is altered. We also test if lymphatic hyperplasia protects against periodontal disease development. Transgenic keratin 14 (K14)-VEGFC mice had significant hyperplasia of lymphatics in oral mucosa, including gingiva, without changes in blood vessel vasculature. The basal lymph flow was normal but slightly lower than in wild-type mice when oral mucosa was challenged with lipopolysaccharide from Porphyromonas gingivalis. Under normal conditions, K14-VEGFC mice exhibited an increased number of neutrophils in gingiva, demonstrated enhanced phagocyte recruitment in the cervical lymph nodes, and had more alveolar bone when compared with their wild-type littermates. After induction of periodontitis, no strain differences were observed in the periodontal tissues with respect to granulocyte recruitment, bone resorption, angiogenesis, cytokines, and bone-related protein expressions or in draining lymph node immune cell proportions and vascularization. We conclude that overexpression of VEGFC results in hyperplastic lymphatics, which do not enhance lymphatic drainage capacity but facilitate phagocyte transport to draining lymph nodes. Hyperplasia of lymphatics does not protect against development of ligature-induced periodontitis.

The radiographic localization of impacted maxillary canines: a comparison of methods.

This study compared two different radiographic techniques for localization of impacted maxillary canines: vertical parallax (from a panoramic and a maxillary anterior occlusal radiograph) and magnification (from a single panoramic radiograph). The radiographs and the information regarding the impacted canines were obtained retrospectively from records of patients treated in the Day Stay Unit of the Eastman Dental Hospital. The two different radiographic techniques were tested blind and compared for localization of the impacted canine by six examiners. The 'gold standard' used for the radiographic comparisons was the true position of the canine as recorded at operation. The results showed a wide variation between the six examiners in the prediction of the canine position with the two different techniques. Localization with vertical parallax was more successful overall than with magnification, although the difference failed to reach significance. Seventy-six per cent of the impacted canines could be successfully located with vertical parallax and 66 per cent with magnification. Further analysis showed that, while almost 90 per cent of the palatally impacted canines could be correctly detected with both techniques, less than half of the buccal canines could be detected with parallax and only one in 10 buccal canines could be detected with magnification. If a canine is suspected to be buccally placed from its appearance on a panoramic film and cannot be palpated, further views are justified.