RESUMO
Chromatin packaging in mammalian spermatozoa requires an ordered replacement of the somatic histones by two classes of spermatid-specific basic proteins, the transition proteins and the protamines. Temporal expression of transition proteins and protamines during spermatid differentiation is under translational control, and premature translation of protamine 1 leads to precocious nuclear condensation and sterility. We have previously suggested that the double-stranded (ds) RNA binding protein Prbp (encoded by the gene Tarbp2) functions as a translational regulator during mouse spermatogenesis. Here we show that Prbp is required for proper translational activation of the mRNAs encoding the protamines. We generated mice that carry a targeted disruption of Tarbp2 and determined that they were sterile and severely oligospermic. Using immunohistological analysis, we determined that the endogenous Prm2 mRNA and a reporter mRNA carrying protamine 1 translational-control elements were translated in a mosaic pattern. We showed that failure to synthesize the protamines resulted in delayed replacement of the transition proteins and subsequent failure of spermiation. The timing of Prbp expression suggests that it may function as a chaperone in the assembly of specific translationally regulated ribonucleoprotein particles.
Assuntos
Proteínas de Ligação a RNA/genética , Proteínas de Ligação a RNA/metabolismo , Testículo/metabolismo , Animais , Peso Corporal , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Endogâmicos , Camundongos Knockout , Tamanho do Órgão , Contagem de Espermatozoides , Testículo/anatomia & histologia , Testículo/citologiaRESUMO
ATM is a member of the phosphatidylinositol 3-kinase (PIK)-like kinases, some of which are active in regulating DNA damage-induced mitotic cell-cycle checkpoints. ATM also plays a role in meiosis. Spermatogenesis in Atm-/- male mice is disrupted, with chromosome fragmentation leading to meiotic arrest; in human patients with ataxia-telangiectasia (A-T), gonadal atrophy is common. Immuno-localization studies indicate that ATM is associated with sites along the synaptonemal complex (SC), the specialized structure along which meiotic recombination occurs. Recombination, preceded by pairing of homologous chromosomes, is thought to require heteroduplex formation between homologous DNA, followed by strand exchange. These early meiotic steps (entailing the formation and processing of meiotic recombination intermediates with DNA-strand interruptions) require ssDNA-binding proteins such as replication protein A (RPA; refs 5-7). In somatic cells, DNA damage induces ATM-dependent phosphorylation of RPA. We demonstrate here that ATM and RPA co-localize along synapsed meiotic chromosomes and at sites where interactions between ectopic homologous chromosome regions appear to initiate. In Atm-/- meiotic prophase spermatocytes, immuno-localization shows that RPA is present along synapsing chromosomes and at sites of fragmentation of the SC. These results suggest that RPA and ATM co-localize at sites where interhomologous-DNA interactions occur during meiotic prophase and where breaks associated with meiotic recombination take place after synapsis, implying a possible functional interaction between these two proteins.
Assuntos
Ataxia Telangiectasia/genética , Proteínas de Ligação a DNA/genética , Meiose/genética , Proteínas Serina-Treonina Quinases , Proteínas/genética , Recombinação Genética , Animais , Ataxia Telangiectasia/enzimologia , Proteínas Mutadas de Ataxia Telangiectasia , Proteínas de Ciclo Celular , Fragmentação do DNA/genética , Humanos , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Knockout , Fosfatidilinositol 3-Quinases/genética , Prófase/genética , Proteína de Replicação A , Espermatócitos/citologia , Espermatócitos/enzimologia , Espermatócitos/metabolismo , Complexo Sinaptonêmico/genética , Proteínas Supressoras de TumorRESUMO
Higher-order chromatin has been implicated in epigenetic gene control and in the functional organization of chromosomes. We have recently discovered mouse (Suv39h1) and human (SUV39H1) histone H3 lysine 9-selective methyltransferases (Suv39h HMTases) and shown that they modulate chromatin dynamics in somatic cells. We describe here the isolation, chromosomal assignment, and characterization of a second murine gene, Suv39h2. Like Suv39h1, Suv39h2 encodes an H3 HMTase that shares 59% identity with Suv39h1 but which differs by the presence of a highly basic N terminus. Using fluorescent in situ hybridization and haplotype analysis, the Suv39h2 locus was mapped to the subcentromeric region of mouse chromosome 2, whereas the Suv39h1 locus resides at the tip of the mouse X chromosome. Notably, although both Suv39h loci display overlapping expression profiles during mouse embryogenesis, Suv39h2 transcripts remain specifically expressed in adult testes. Immunolocalization of Suv39h2 protein during spermatogenesis indicates enriched distribution at the heterochromatin from the leptotene to the round spermatid stage. Moreover, Suv39h2 specifically accumulates with chromatin of the sex chromosomes (XY body) which undergo transcriptional silencing during the first meiotic prophase. These data are consistent with redundant enzymatic roles for Suv39h1 and Suv39h2 during mouse development and suggest an additional function of the Suv39h2 HMTase in organizing meiotic heterochromatin that may even impart an epigenetic imprint to the male germ line.
Assuntos
Cromatina/genética , Histona-Lisina N-Metiltransferase , Metiltransferases/genética , Metiltransferases/metabolismo , Fosfoproteínas/genética , Testículo/metabolismo , Sequência de Aminoácidos , Animais , Sequência de Bases , Células Cultivadas , Cromatina/metabolismo , Mapeamento Cromossômico , Clonagem Molecular , Embrião de Mamíferos/metabolismo , Fibroblastos , Expressão Gênica , Células Germinativas/metabolismo , Células HeLa , Histona Metiltransferases , Humanos , Immunoblotting , Hibridização in Situ Fluorescente , Masculino , Metiltransferases/química , Camundongos , Camundongos Endogâmicos C57BL , Microscopia de Fluorescência , Dados de Sequência Molecular , Fosfoproteínas/metabolismo , Filogenia , Proteínas Metiltransferases , RNA/metabolismo , Cromossomos Sexuais/metabolismo , Testículo/anatomia & histologia , Testículo/químicaRESUMO
Epigenetic mechanisms control eukaryotic development beyond DNA-stored information. DNA methylation, histone modifications and variants, nucleosome remodeling and noncoding RNAs all contribute to the dynamic make-up of chromatin under distinct developmental options. In particular, the great diversity of covalent histone tail modifications has been proposed to be ideally suited for imparting epigenetic information. While most of the histone tail modifications represent transient marks at transcriptionally permissive chromatin, some modifications appear more robust at silent chromatin regions, where they index repressive epigenetic states with functions also outside transcriptional regulation. Under-representation of repressive histone marks could be indicative of epigenetic plasticity in stem, young and tumor cells, while committed and senescent (old) cells often display increased levels of these more stable modifications. Here, we discuss profiles of normal and aberrant histone lysine methylation patterns, as they occur during the transition of an embryonic to a differentiated cell or in controlled self-renewal vs pro-neoplastic or metastatic conditions. Elucidating these histone modification patterns promises to have important implications for novel advances in stem cell research, nuclear reprogramming and cancer, and may offer novel targets for the combat of tumor cells, potentially leading to new diagnostic and therapeutic avenues in human biology and disease.
Assuntos
Cromatina/genética , Cromatina/metabolismo , Epigênese Genética/genética , Histonas/genética , Histonas/metabolismo , Animais , Diferenciação Celular/genética , Linhagem da Célula/genética , Transformação Celular Neoplásica/genética , Desenvolvimento Embrionário/genética , Humanos , Metilação , Estrutura Terciária de Proteína/fisiologiaRESUMO
The development of multi-cellular organisms is regulated by the ordered definition of gene expression programmes that govern cell proliferation and differentiation. Although differential gene activity is mainly controlled by transcription factors, it is also dependent upon the underlying chromatin structure, which can stabilize transcriptional "on" or "off" states. We have recently isolated human (SUV39H1) and mouse (Suv39h1) histone methyltransferases (HMTases) and shown that they are important regulators for the organization of repressive chromatin domains. To investigate whether a SUV39H1-induced modulation of heterochromatin would affect mammalian development, we generated transgenic mice that over-express the SUV39H1 HMTase early during embryogenesis. SUV39H1 transgenic mice are growth retarded, display a weak penetrance of skeletal transformations and are largely characterized by impaired erythroid differentiation, consistent with highest transgene expression in foetal liver. Ex vivo transgenic foetal liver cultures initially contain reduced numbers of cells in G1 but progress to immortalized erythroblasts that are compromised in executing an erythroid differentiation programme. The outgrowing SUV39H1-immortalized erythroblasts can maintain a diploid karyotype despite deregulation of several tumour suppressor proteins and dispersed distribution of the heterochromatin component HP1. Together, these data provide evidence for a role of the SUV39H1 HMTase during the mammalian development and indicate a possible function for higher-order chromatin in contributing to the balance between proliferation and differentiation potentials of progenitor cells.
Assuntos
Diferenciação Celular , Divisão Celular , Eritroblastos/fisiologia , Eritropoese , Metiltransferases/metabolismo , Proteínas Repressoras/metabolismo , Animais , Peso Corporal , Osso e Ossos/anormalidades , Ciclo Celular , Células Cultivadas , Homólogo 5 da Proteína Cromobox , Proteínas Cromossômicas não Histona/metabolismo , Desenvolvimento Embrionário e Fetal , Expressão Gênica , Heterocromatina/metabolismo , Histonas/metabolismo , Cariotipagem , Fígado/citologia , Fígado/embriologia , Fígado/enzimologia , Metiltransferases/genética , Camundongos , Camundongos Transgênicos , Penetrância , Proteínas Repressoras/genética , Proteína do Retinoblastoma/metabolismo , Baço/citologia , Baço/enzimologia , Transgenes , Proteína Supressora de Tumor p53/metabolismoRESUMO
Regulation of androgen receptor (AR) mRNA expression was studied in Sertoli cells and peritubular myoid cells isolated from immature rat testis, and in the lymph node carcinoma cell line derived from a human prostate (LNCaP). Addition of dibutyryl-cyclic AMP (dbcAMP) to Sertoli cell cultures resulted in a rapid transient decrease in AR mRNA expression (5 h), which was followed by a gradual increase in AR mRNA expression (24-72 h). This effect of dbcAMP mimicked follicle-stimulating hormone (FSH) action. In peritubular myoid cells, there was only a moderate but prolonged decrease during incubation in the presence of dbcAMP, and in LNCaP cells no effect of dbcAMP on AR mRNA expression was observed. When Sertoli cells or peritubular myoid cells were cultured in the presence of androgens, AR mRNA expression in these cell types did not change. This is in contrast to LNCaP cells, that showed a marked reduction of AR mRNA expression during androgen treatment. In the present experiments, transcriptional regulation of AR gene expression in Sertoli cells and LNCaP cells was also examined. Freshly isolated Sertoli cell clusters were transfected with a series of luciferase reporter gene constructs, driven by the AR promoter. It was found that addition of dbcAMP to the transfected Sertoli cells resulted in a small but consistent increase in reporter gene expression (which was interpreted as resulting from AR promoter activity); a construct that only contained the AR 5' untranslated region of the cDNA sequence did not show such a regulation. The same constructs, transfected into LNCaP cells, did not show any transcriptional down-regulation when the synthetic androgen R1881 was added to the cell cultures. A nuclear transcription elongation experiment (run-on), however, demonstrated that androgen-induced AR mRNA down-regulation in LNCaP cells resulted from an inhibition of AR gene transcription. The present results indicate that in Sertoli cells and LNCaP cells, hormonal effects on AR gene transcription play a role in regulation of AR expression. However, AR gene transcription in these cells is differentially regulated.
Assuntos
Regulação da Expressão Gênica , Músculo Liso/metabolismo , Receptores Androgênicos/biossíntese , Células de Sertoli/metabolismo , Transcrição Gênica , Animais , Bucladesina/farmacologia , Células Cultivadas , Hormônio Foliculoestimulante/farmacologia , Regulação da Expressão Gênica/efeitos dos fármacos , Humanos , Metástase Linfática/patologia , Masculino , Metribolona/farmacologia , Neoplasias da Próstata/patologia , RNA Mensageiro/biossíntese , Ratos , Receptores Androgênicos/genética , Proteínas Recombinantes de Fusão/biossíntese , Testículo/citologia , Transcrição Gênica/efeitos dos fármacos , Transfecção , Células Tumorais CultivadasAssuntos
Desenvolvimento Ósseo , Retardo do Crescimento Fetal/fisiopatologia , Epífises/diagnóstico por imagem , Epífises/crescimento & desenvolvimento , Feminino , Fíbula/diagnóstico por imagem , Fíbula/crescimento & desenvolvimento , Idade Gestacional , Humanos , Lactente , Recém-Nascido , Masculino , RadiografiaRESUMO
Mice that are double heterozygous for the semi-identical T(1;13)70H and T(1;13)1Wa reciprocal translocations display a great variation in male fertility. The synaptic behaviour of the different translocation chromosomes of adult males was studied in relation to this parameter. Juvenile males and embryonic females (16 and 18 days old) were included for comparison. In agreement with the minor differences In the translocation breakpoint positions, two differently sized heteromorphic bivalents are formed in meiotic prophase of both sexes (a quadrivalent was never encountered). Synaptonemal complex (SC) configurations of both bivalents in either sex are characterized by a high degree of non-homologous synapsis at zygotene-early pachytene. The rate of synaptic adjustment during pachytene is dependent on the size of the heteromorphic bivalent and varies between the sexes. Differences in SC configuration and morphology of the small heteromorphic bivalent in particular exist between the sexes and between animals. In males, this correlates with different degrees of fertility. Normal SC morphology in a fully synapsed small heteromorphic bivalent is an important determinant of successful meiosis and spermatogenesis. Moreover, aberrant synapsis favours the 'unsaturated pairing site' model as the primary cause for male sterility.
Assuntos
Fertilidade/genética , Meiose/genética , Translocação Genética , Animais , Bandeamento Cromossômico , Cromossomos , Feminino , Infertilidade Masculina/genética , Masculino , Camundongos , Prófase , Espermatogênese/genéticaRESUMO
Translational regulation of the protamine 1 mRNA is mediated by sequences in its 3' untranslated region. In this study, we demonstrate that a highly conserved sequence, the translational control element, is solely responsible for protamine 1 translational regulation. Mutation of the conserved sequence causes premature translation of a transgene containing a fusion between the human growth hormone coding sequence and the protamine 1 3' untranslated region. Temporal expression of the transgene was monitored in prepubertal animals by Northern and Western blotting and in adult animals by immunocytochemistry. Messenger RNAs lacking the translational control element sediment in the messenger ribonucleoprotein particle and ribosomal fractions of polysome gradients, suggesting that the translational control element is required for translational repression but not for incorporation of mRNAs into ribonucleoprotein particles.
Assuntos
Sequência Conservada , Regulação da Expressão Gênica , Protaminas/genética , Biossíntese de Proteínas , RNA Mensageiro/análise , Espermátides/metabolismo , Acrossomo/metabolismo , Animais , Sequência de Bases , Northern Blotting , Western Blotting , Hormônio do Crescimento Humano/genética , Humanos , Imuno-Histoquímica , Masculino , Camundongos , Camundongos Transgênicos , Dados de Sequência Molecular , Mutação , Proteínas Recombinantes de FusãoRESUMO
The objective of this study was to determine whether fully grown oocytes, obtained after isolation from preantral follicles and growth in vitro, secrete paracrine factors affecting granulosa cell development and function. If so, the relative ease in producing oocytes in this way could facilitate the identification and characterization of the factors. As a test of this idea, the ability of in vitro grown oocytes to produce a paracrine factor that is known to enable the isolated cumulus oophorus to undergo expansion in response to follicle stimulating hormone (FSH) was determined. Initial experiments compared culture systems, which differed in the orientation of the oocyte-granulosa cell complexes from preantral follicles to an extracellular matrix, for their ability to support oocyte growth and the acquisition of competence to resume meiosis. The systems for culture on the surface of the matrix produced larger oocytes and the highest percentage of oocytes having competence to resume meiosis. Oocytes grown using this system secreted active cumulus expansion enabling factor, albeit at levels about half that of oocytes grown in vivo. A preliminary characterization of the cumulus expansion enabling factor secreted by the oocytes grown in vitro showed that activity was lost upon treatment with either heat (65 degrees C for 15 min) or proteinase K. Activity did not pass through a membrane having a nominal molecular weight limit (NMWL) of 100 kd but did pass through a membrane having a NMWL of 300 kd. It is concluded that cumulus expansion enabling factor is secreted by oocytes grown in vitro. This factor is probably a protein or depends upon a protein for its activity.(ABSTRACT TRUNCATED AT 250 WORDS)
Assuntos
Células da Granulosa/citologia , Substâncias de Crescimento/biossíntese , Oócitos/metabolismo , Animais , Divisão Celular , Meios de Cultivo Condicionados , Endopeptidases , Feminino , Substâncias de Crescimento/isolamento & purificação , Temperatura Alta , Técnicas In Vitro , Camundongos , Camundongos Endogâmicos C57BL , Oócitos/crescimento & desenvolvimentoRESUMO
Homologous chromosome synapsis and meiotic recombination are facilitated by several meiosis-specific structures: the synaptonemal complex (SC), and two types of meiotic nodules: (1) early meiotic nodules (MNs), also called zygotene nodules or early recombination nodules, and (2) late recombination nodules (RNs). The former are thought to be nucleoprotein complexes involved in the check for homology preceding, or accompanying synapsis, while the latter have been shown to be involved in reciprocal recombination. We have examined by immunocytochemistry the meiotic localization of a series of proteins at sites along the asynapsed axial elements prior to homologous synapsis and at sites along the SCs following synapsis. Several of the proteins examined have been implicated in repair/recombination and include RAD51, a mammalian homolog of the Escherichia coli RecA protein; Replication Protein-A (RPA), a single-strand DNA binding protein; and MLH1, a mismatch repair protein which is a homolog of the E. coli MutL protein. In addition two proteins were examined that have been implicated in meiotic checkpoints: ATM, the protein mutated in the human disease Ataxia Telangiectasia, and ATR, another member of the same family of PIK kinases. We present evidence that these proteins are all components of meiotic nodules and document changes in protein composition of these structures during zygonema and pachynema of meiotic prophase in mouse spermatocytes. These studies support the supposition that a subset of MNs are converted into RNs. However, our data also demonstrate changes in protein composition within the context of early MNs, suggesting a differentiation of these nodules during the process of synapsis. The same changes in protein composition occurred on both the normal X axis, which has no homologous pairing partner in spermatocytes, and on the axes of aberrant chromosomes that nonhomologously synapse during synaptic adjustment. These findings suggest that DNA sequences associated with MNs still must undergo an obligatory processing, even in the absence of interactions between homologous chromosomes.
Assuntos
Proteínas de Ligação a DNA/análise , Meiose , Nucleoproteínas/química , Proteínas Serina-Treonina Quinases , Proteínas/análise , Animais , Proteínas Mutadas de Ataxia Telangiectasia , Proteínas de Ciclo Celular/análise , Cromossomos/química , Reparo do DNA , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Rad51 Recombinase , Recombinação Genética , Proteína de Replicação A , Espermatócitos , Translocação Genética , Proteínas Supressoras de TumorRESUMO
The possibility of inadvertent exposure of gonadal tissue to gene therapy vectors has raised safety concerns about germline infection. We show here that the receptor for coxsackie B viruses and adenoviruses 2 and 5 (CXADR) is expressed in mouse germ cells, suggesting the possibility that these viruses could infect germ cells. To directly assess the risk of germline infection in vivo, we injected an adenovirus carrying the germ-cell-specific protamine promoter fused to the bacterial lacZ reporter gene into the left ventricular cavity of mice and then monitored expression of the reporter gene in germ cells. To differentiate between infection of stem cells and differentiating spermatogenic cells, we analyzed expression of the reporter cassette at different times after viral delivery. Under all conditions tested, mice did not express the Escherichia coli beta-galactosidase protein in developing spermatids or in mature epididymal spermatozoa. Primary germ cells cultured in vitro were also refractory to adenoviral infection. Our data suggest that the chance of vertical germline transmission and insertional mutagenesis is highly unlikely following intracoronary adenoviral delivery.
Assuntos
Adenoviridae/fisiologia , Ventrículos Cerebrais/virologia , Terapia Genética/métodos , Receptores Virais/metabolismo , Espermatozoides/virologia , Testículo/virologia , Animais , Proteína de Membrana Semelhante a Receptor de Coxsackie e Adenovirus , Primers do DNA/química , Técnica Indireta de Fluorescência para Anticorpo , Técnicas de Transferência de Genes , Humanos , Injeções Intraventriculares , Óperon Lac , Masculino , Proteínas de Membrana/genética , Camundongos , Reação em Cadeia da Polimerase , Espermatozoides/metabolismo , Testículo/metabolismo , beta-Galactosidase/metabolismoRESUMO
Histone H3 lysine 9 methylation has been proposed to provide a major "switch" for the functional organization of chromosomal subdomains. Here, we show that the murine Suv39h histone methyltransferases (HMTases) govern H3-K9 methylation at pericentric heterochromatin and induce a specialized histone methylation pattern that differs from the broad H3-K9 methylation present at other chromosomal regions. Suv39h-deficient mice display severely impaired viability and chromosomal instabilities that are associated with an increased tumor risk and perturbed chromosome interactions during male meiosis. These in vivo data assign a crucial role for pericentric H3-K9 methylation in protecting genome stability, and define the Suv39h HMTases as important epigenetic regulators for mammalian development.
Assuntos
Segregação de Cromossomos/fisiologia , Heterocromatina/fisiologia , Histona-Lisina N-Metiltransferase/fisiologia , Metiltransferases/fisiologia , Proteínas Repressoras/fisiologia , Aberrações dos Cromossomos Sexuais , Aneuploidia , Animais , Fibroblastos/citologia , Marcação de Genes/métodos , Genoma , Células Germinativas , Histona Metiltransferases , Histona-Lisina N-Metiltransferase/genética , Histonas/metabolismo , Hipogonadismo , Linfoma de Células B , Masculino , Mamíferos , Meiose , Metilação , Metiltransferases/genética , Camundongos , Camundongos Knockout , Camundongos Mutantes , Mutagênese , Proteínas Metiltransferases , Proteínas Repressoras/genética , Espermatócitos , Espermatogênese/genética , Espermatogênese/fisiologiaRESUMO
The lateral elements (LEs) of synaptonemal complexes (SCs) of the rat contain major components with relative electrophoretic mobilities (Mr s) of 30000-33000, which are the products of a single gene. After one-dimensional separation of SC proteins on polyacrylamide-SDS gels, these components show up as two major bands, whereas upon two-dimensional electrophoresis they are resolved in at least 24 spots, which focus at pH 6.5 to 9.5. In this paper we show that these spots represent phosphorylation variants. For the analysis of the phosphorylation of the 30000- to 33000-Mr SC components during progression through meiotic prophase, we developed a procedure for isolation of fractions of testicular cells of the rat that are enriched in separate stages of meiotic prophase. Analysis of the 30000- to 33000-Mr SC components in these fractions by two-dimensional electrophoresis and immunoblotting showed that phosphorylated variants of the 30000- to 33000-Mr SC proteins occur throughout meiotic prophase. However, the extent of phosphorylation changes between early and mid-pachytene, when one phosphate group is probably added to each of the variants.