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1.
Eur J Med Genet ; 64(10): 104293, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34352413

RESUMO

Bloom Syndrome (BS) is a genetic DNA repair disorder, caused by mutations in the BLM gene. The clinical phenotype includes growth retardation, immunodeficiency and a strong predisposition to different types of malignancies. Treatment of malignancies in BS patients with radiotherapy or chemotherapy is believed to be associated with increased toxicity, but clinical and laboratory data are lacking. We collected clinical data of two Dutch BS patients with solid tumors. Both were treated with radiotherapy before the diagnosis BS was made and tolerated this treatment well. In addition, we collected fibroblasts from BS patients to perform in vitro clonogenic survival assays to determine radiosensitivity. BS fibroblasts showed less radiosensitivity than the severely radiosensitive Artemis fibroblasts. Moreover, studies of double strand break kinetics by counting 53BP1 foci after irradiation showed similar patterns compared to healthy controls. In combination, the clinical cases and laboratory experiments are valuable information in the discussion whether radiotherapy is absolutely contraindicated in BS, which is the Case in other DNA repair syndromes like Ataxia Telangiectasia and Artemis.


Assuntos
Síndrome de Bloom/complicações , Carcinoma/radioterapia , Radioterapia/efeitos adversos , Adulto , Síndrome de Bloom/genética , Carcinoma/complicações , Células Cultivadas , Quebras de DNA de Cadeia Dupla , Reparo do DNA , Feminino , Fibroblastos/efeitos da radiação , Humanos , Masculino , Pessoa de Meia-Idade , Tolerância a Radiação , RecQ Helicases/genética
2.
Free Radic Res ; 25(4): 321-36, 1996 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-8889496

RESUMO

The human hepatoblastoma cell line HepG2 is a liver model commonly used for lipid metabolism studies. Numerous cell types have been found to oxidize low-density lipoprotein (LDL) but, to our knowledge, the effects of HepG2 cells on LDL have not been investigated. We found that LDL is modified by HepG2 cells through a peroxidative mechanism, as judged by an increase in TBARS content (which was prevented in the presence of the antioxidants vitamin E, 2,6-di-tertbutyl-cresol and probucol), increased degradation by J774 macrophages, decreased internalization by MRC5 fibroblasts, and aggregation of apo B. Aspirin and allopurinol, which inhibit cyclooxygenase and xanthine-oxidase activities, respectively, had no effect on HepG2-induced LDL modification, and neither did catalase, which dismutates hydrogen peroxide; or mannitol, which scavenges hydroxyl radicals. In contrast, superoxide dismutase, a superoxide anion scavenger, and glutamate and threonine, which alter cellular cystine uptake, prevented LDL modifications, as did the removal of cysteine/cystine from the culture medium. Oxidation of LDL by HepG2 cells might thus involve superoxide anion production and/or thiol metabolism.


Assuntos
Hepatoblastoma/metabolismo , Lipoproteínas LDL/metabolismo , Neoplasias Hepáticas/metabolismo , Antioxidantes/farmacologia , Hidroxitolueno Butilado/farmacologia , Cromatografia por Troca Iônica , Eletroforese em Gel de Ágar , Eletroforese em Gel de Poliacrilamida , Hepatoblastoma/tratamento farmacológico , Hepatoblastoma/patologia , Humanos , Peroxidação de Lipídeos , Lipoproteínas LDL/química , Lipoproteínas LDL/efeitos dos fármacos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/patologia , Oxirredução/efeitos dos fármacos , Probucol/farmacologia , Superóxidos/metabolismo , Substâncias Reativas com Ácido Tiobarbitúrico/metabolismo , Células Tumorais Cultivadas , Vitamina E/farmacologia
3.
J Pharmacol Toxicol Methods ; 30(2): 69-73, 1993 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-8298183

RESUMO

We used a recently described anion-exchange chromatographic method (Vedie et al. J Lipid Res 1991;32:1359) to study the protective effect of potential inhibitors of low-density lipoprotein (LDL) oxidation mediated by cupric ion. By way of an example, we studied eight flavonoids (flavone, 3-hydroxyflavone, chrysin, galangin, fisetin, morin, quercetin, and myricetin) as well as three non-flavonoid antioxidants, butylated hydroxytoluene (BHT), probucol, and vitamin C, as reference compounds. Each compound was tested at various concentrations (1-100 microM). For flavonoid concentrations of 10 microM, an index was calculated as the (LDL control-flavonoid)/(LDL control-probucol) ratio, in which each term is expressed as the percentage of the most electronegative LDL fraction (fraction E). If the index is positive, the flavonoid inhibits LDL oxidation. A value > 1 (3-hydroxyflavone and galangin) means greater activity than probucol, whereas a value < 1 means lower activity (fisetin). If the index is around 0 (flavone and chrysin), the flavonoid is inactive. Finally, a negative value reflects possible prooxidant activity (morin, quercetin, and myricetin). Our results show that this chromatographic method can be applied to screening new pharmacological agents for activity against LDL oxidation.


Assuntos
Antioxidantes/farmacologia , Flavonoides/farmacologia , Lipoproteínas LDL/metabolismo , Relação Dose-Resposta a Droga , Humanos , Lipoproteínas LDL/efeitos dos fármacos , Relação Estrutura-Atividade
4.
J Reprod Med ; 44(10): 891-3, 1999 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-10554753

RESUMO

BACKGROUND: Primary amenorrhea and lack of sexual development occur in gonadal dysgenesis due to missing ovaries. Primary amenorrhea with sexual development occurs in Rokitansky syndrome due to absence of the uterus, with normal ovarian function. The association of these two conditions has been previously described as a rare event. CASE: A 19-year-old woman presented with primary amenorrhea and lack of secondary sexual characteristics. Physical examination confirmed the absence of mammary development and of pubic and axillary hair. Pelvic ultrasound disclosed absence of the uterus and ovaries. Gonadotropin serum levels were in the menopausal range, and the karyotype showed two mosaic cell lines, 45,X/46,Xdic(X). Scanning of a large number of cells by interphase fluorescence in situ hybridization showed 12% of cells with a dicentric X chromosome. Laparoscopic study confirmed the absence of the uterus and ovaries, with normal fallopian tubes. CONCLUSION: This patient had two anomalies affecting reproductive performance, gonadal dysgenesis and congenital absence of the uterus, the first associated with an abnormal karyotype; the second seems to have occurred coincidentally. At this time there is no treatment for the reproductive dysfunction.


Assuntos
Amenorreia/etiologia , Transtornos do Desenvolvimento Sexual/etiologia , Disgenesia Gonadal/genética , Ovário/anormalidades , Útero/anormalidades , Adulto , Aberrações Cromossômicas , Transtornos Cromossômicos , Feminino , Humanos , Cariotipagem , Ovário/patologia , Síndrome , Útero/patologia , Cromossomo X
5.
Ann Biol Clin (Paris) ; 54(6): 229-34, 1996.
Artigo em Francês | MEDLINE | ID: mdl-8949419

RESUMO

Traditionally, low-density lipoprotein (LDL) are separated with respect to their size, density and apolipoprotein composition. Fractionation of LDL according to their electrical charge is also interesting as modified LDL have been implicated in the onset of atherosclerosis. This review discusses possible mechanisms underlying charge heterogeneity of human plasma LDL, such as oxidation, glycation, conjugation with aldehydes, carbamylation and changes in sialic acid content and protein composition.


Assuntos
Lipoproteínas LDL/química , Aldeídos/metabolismo , Heterogeneidade Genética , Glicosilação , Humanos , Técnicas In Vitro , Lipoproteínas LDL/genética , Lipoproteínas LDL/metabolismo , Oxirredução , Ácidos Siálicos/química
9.
Experientia ; 52(3): 234-8, 1996 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-8631392

RESUMO

Modifications of LDL by the EA.hy 926 cell line were compared to those generated by human umbilical vein endothelial cells (HUVEC). Thiobarbituric acid reactive substances (TBARS) index values (TBARS sample/TBARS cell-free control ratio) were 2.64 +/- 0.18 (m +/- SE, n = 11) and 3.12 +/- 0.24 (n = 11), for HUVEC and EA.hy 926, respectively. The percentage of the most electronegative modified LDL fraction (fraction C), assessed by using an ion-exchange chromatographic method based on fast protein liquid chromatography (FPLC), represented 14 +/- 3% (n = 34) and 22 +/- 13% (n =10) of total modified LDL in HUVEC and EA.hy 926, respectively. LDL modified by both cell lines showed increased agarose electrophoretic mobility and apo B100 fragmentation on SDS-PAGE. None of the results were significantly different between the two cell lines. Superoxide anion production was 0.12 +/- 0.04 (n = 11) and 0.07 +/- 0.01 nmol/min/mg cell protein (n = 11) in HUVEC and EA.hy 926, respectively. Cell-specific effects on LDL were abrogated in cysteine-free medium. Moreover, cell-modified LDL were similarly degraded by J774 macrophage-like cells. We conclude that EA.hy 926 cells are a good model for investigating endothelial cell-induced modifications of LDL. Advantages include ready availability and less individual variability than with HUVEC.


Assuntos
Endotélio Vascular/metabolismo , Lipoproteínas LDL/metabolismo , Linhagem Celular , Cisteína/metabolismo , Cistina/metabolismo , Humanos , Macrófagos/metabolismo , Oxirredução , Superóxidos/metabolismo
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