RESUMO
Bacillus subtilis phage SPR codes for a DNA methyltransferase (Mtase) which methylates the 5' cytosine in the sequence GGCC and both cytosines in the sequence CCGG. A 2126-bp fragment of SPR DNA containing the Mtase gene has been sequenced. This fragment has only one significant open reading frame of 1347 bp, which corresponds to the Mtase gene. Within the sequence the Mtase promoter has been defined by S1 mapping. The size of the SPR Mtase predicted from the deduced amino acid composition is 49.9 kDal. This is in agreement with both the Mr of the purified enzyme and with that of the SPR Mtase gene product identified here by minicell technique. Base changes leading to mutants affected in Mtase activity were localized within the Mtase gene.
Assuntos
Bacillus subtilis/genética , Bacteriófagos/genética , DNA (Citosina-5-)-Metiltransferases/genética , Genes Virais , Genes , Metiltransferases/genética , Sequência de Aminoácidos , Bacillus subtilis/enzimologia , Bacteriófagos/enzimologia , Sequência de Bases , DNA (Citosina-5-)-Metiltransferases/isolamento & purificação , Enzimas de Restrição do DNA , Peso Molecular , Plasmídeos , Transcrição GênicaRESUMO
Two children with spinal dermal sinuses of congenital origin are described. They are unusual because the skin dimples were located off the midline at the gluteal region.