Combined aerobic resistance exercise improves dialysis adequacy and quality of life in patients on maintenance hemodialysis
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OBJECTIVE: This study aims to compare the effect of pure aerobic exercise and combined aerobic resistance exercise on dialysis adequacy and quality of life in patients on maintenance hemodialysis. MATERIALS AND METHODS: A total of 45 patients on maintenance hemodialysis were divided into three groups: pure aerobic exercise group, combined aerobic resistance exercise group, and control group. Patients in the control group were only given the usual treatment, which included dietary guidance, drug therapy, and hemodialysis. The other training groups underwent 12-week exercise intervention therapy on the basis of the usual treatment. Blood samples were collected before and after the hemodialysis, at the beginning and end of the intervention for these three groups. Then, the blood urea nitrogen (BUN) concentration was determined, the urea clearance index (Kt/v) and urea degradation rate (URR) were calculated, the dialysis adequacy was evaluated, and the short form-36 (SF-36) scale was used to evaluate the life quality. RESULTS: Before intervention, there was no significant difference in general health condition (GH), Kt/v, URR, SF-36 total score, and the score of each dimension in the three groups. After the intervention therapy, the Kt/v, URR, GH, vitality (VT), and SF-36 total score markedly improved in the pure aerobic exercise group, while the Kt/v, URR, GH, VT, physical functioning (PF), and SF-36 total score significantly increased in the combined aerobic resistance exercise group. Furthermore, compared with the pure aerobic exercise group, the improvement effect of body function (PF score) was better in the combined aerobic resistance exercise group. CONCLUSION: Both pure aerobic exercise and combined aerobic resistance exercise can significantly improve the dialysis adequacy and quality of life of maintenance hemodialysis patients. Compared with the pure aerobic exercise group, the effect of combined aerobic resistance exercise on PF was better.
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[Analysis on formula of Mongolian medicine for prevention of COVID-19].

The epidemic situation of coronavirus disease 2019(COVID-19) is developing rapidly in the world, and the influence is serious. In this study, the prescription of Mongolian medicine to prevent new type of COVID-19 was investigated. Based on the second edition and the third edition of COVID-19 Mongolian Medicine Prevention and Treatment Guidance Program issued by the Inner Mongolia Autonomous Region Health Commission, using Excel 2007, SPSS Modeler 18, SPSS Statistics 25, Cytoscape 3.7.1 statistical software as a tool, the association rules analysis and cluster analysis of Mongolian medicine included in the standard were carried out. Among the 45 prophylactic prescriptions included in the standard, a total of 34 high-frequency drugs using frequency ≥5 were used, of which Carthami Flos(21 times, 4.46%), Chebulae Fructus(20 times, 4.26%), Moschus(13 times, 2.77%), Myristicae Semen(12 times, 2.55%), Santali Albi Lignum(12 times, 2.55%), and Bovis Calculus(12 times, 2.55%) were the most common. The main drugs for the prevention of COVID-19 were Liang(13 times, 38.23%), Wen(9 times, 26.47%), the flavor was Ku(20 times, 34.48%), Xin(13 times, 22.41%), Gan(11 times, 18.97%), the most used drugs treating hot evil(99 times, 32.46%), treatment of "Heyi" drugs(51 times, 16.72%), treatment of "Badagan" drugs(40 times, 13.11%), treatment of "sticky" drugs(37 times, 12.13%), and a cough, eliminating phlegm and antiasthmatic(31 times, 10.16%), the association rule analysis found that the highest association intensity of the drug pair combination of 11. Clustering analysis using the cluster analysis of inter-group join method found a total of 8 categories. In this study, 45 prescriptions of Mongolian medicine for the prevention of COVID-19 were collec-ted and further analyzed, hoping to provide new ideas for clinical diagnosis and treatment.

Discovery of diagnosis pattern of coronary heart disease with qi deficiency syndrome by the T-test-based adaboost algorithm.

Coronary heart disease (CHD) is still the leading cause of death for adults worldwide. Traditional Chinese medicine (TCM) has a history of 1000 years fighting against the disease and provides a complementary and alternative treatment to it. Syndrome is the core of TCM diagnosis and it is traditionally diagnosed based on macroscopic symptoms as well as tongue and pulse recognitions of patients. Establishment of the diagnosis method in the microcosmic level is an urgent and major problem in TCM. The aim of this study was to establish characteristic diagnosis pattern for CHD with Qi deficiency syndrome (QDS). Thirty-four biological parameters were detected in 52 patients having unstable angina (UA) with or without QDS. Then, we presented a novel data mining method, t-test-based Adaboost algorithm, to establish highest prediction accuracy with the least number of biological parameters for UA with QDS. We gained a pattern composed of five biological parameters that distinguishes UA with QDS patients from non-QDS patients. The diagnosis accuracy of the patterns could reach 84.5% based on a 3-fold cross validation technique. Moreover, we included 85 UA cases collected from hospitals located in the north and south of China to further verify the association between the pattern and QDS. The classification accuracy is 83.5%, which keeps consistent with the accuracy obtained by the cross-validation technique. The association between a symptom and the five biological parameters was established by the data mining method and it reached an accuracy of ∼80%. These results showed that the t-test-based Adaboost algorithm might be a powerful technique for diagnosing syndrome in TCM in the context of CHD.

Amniocentesis and Next Generation Sequencing (NGS)-Based Noninvasive Prenatal DNA Testing (NIPT) for Prenatal Diagnosis of Fetal Chromosomal Disorders.

OBJECTIVE: The present study aimed to evaluate and analyze the results of karyotyping by amniocentesis and next generation sequencing (NGS)-based noninvasive prenatal DNA testing (NIPT) for the prenatal diagnosis of fetal chromosomal disorders. METHODS: A total of 2267 high-risk pregnant females with the indications for prenatal diagnosis who met the enrollment criteria between January 2015 and May 2019 at the Affiliated Hospital of Inner Mongolia Medical University were included and underwent NGS-based NIPT in the present study. Amniocentesis, chromosome karyotyping by cell culture, and follow-up of the pregnancy outcomes were also conducted in the NIPT-positive pregnant females to assess the consistency between NIPT and results of karyotyping by amniocentesis. RESULTS: Among the 2267 cases, 29 cases were positive for NIPT, including 10 cases with a high risk of trisomy 21, 2 cases with a high risk of trisomy 18, 2 cases with a high risk of chromosome 13, and 20 cases with sex chromosome abnormalities. All the above NIPT-positive cases underwent amniocentesis, and 20 cases were eventually diagnosed. The sensitivity and specificity of NIPT for the diagnosis of trisomy 21, trisomy 13, and trisomy 18 were 100%, 99.96%, 100%, and 99.96%, 100%, 100%, respectively, and the positive predictive values were 91.67%, 66.67%, and 100%, respectively. CONCLUSION: NGS of the fetal free DNA from the peripheral blood of pregnant females was an important complement to the prenatal diagnosis of chromosomal disorders represented by fetal chromosome aneuploidy with high sensitivity and specificity. In combination with the traditional karyotyping by amniocentesis, it could improve the diagnostic efficacy for fetal chromosomal disorders.

Preferential Binding of π-Ligand Porphyrin Targeting 5'-5' Stacking Interface of Human Telomeric RNA G-Quadruplex Dimer.

Human telomeric RNA (TERRA) containing thousands of G-rich repeats has the propensity to form parallel-stranded G-quadruplexes. The emerging crucial roles of TERRA G-quadruplexes in RNA biology fuel increasing attention for studying anticancer ligand binding with such structures, which, however, remains scarce. Here we utilized multiple steady-state and time-resolved spectroscopy analyses in conjunction with NMR methods and investigated thoroughly the binding behavior of TMPyP4 to a TERRA G-quadruplex dimer formed by the 10-nucleotide sequence r(GGGUUAGGGU). It is clearly identified that TMPyP4 intercalates into the 5'-5' stacking interface of two G-quadruplex blocks with a binding stoichiometry of 1:1 and binding constant of 1.92 × 106 M-1. This is consistent with the unique TERRA structural features of the enlarged π-π stacking plane of the A·(G·G·G·G)·A hexad at 5'-ends of each G-quadruplex block. The preferential binding of π-ligand porphyrin to the 5'-5' stacking interface of the native TERRA G-quadruplex dimer is first ascertained by the combination of dynamics and structural characterization.

Potential biodegradation of phenanthrene by isolated halotolerant bacterial strains from petroleum oil polluted soil in Yellow River Delta.

The Yellow River Delta (YRD), being close to Shengli Oilfield, is at high risk for petroleum oil pollution. The aim of this study was to isolate halotolerant phenanthrene (PHE) degrading bacteria for dealing with this contaminates in salinity environment. Two bacterial strains assigned as FM6-1 and FM8-1 were successfully screened from oil contaminated soil in the YRD. Morphological and molecular analysis suggested that strains FM6-1 and FM8-1 were belonging to Delftia sp. and Achromobacter sp., respectively. Bacterial growth of both strains was not dependent on NaCl, however, grew well under extensive NaCl concentration. The optimum NaCl concentration for bacterial production of strain FM8-1 was 4% (m/v), whereas for strain FM6-1, growth was not affected within 2.5% NaCl. Both strains could use the tested aromatic hydrocarbons (naphthalene, phenanthrene, fluoranthene and pyrene) and aliphatic hydrocarbons (C12, C16, C20 and C32) as sole carbon source. The optimized biodegradation conditions for strain FM6-1 were pH 7, 28 °C and 2% NaCl, for strain FM8-1 were pH 8, 28 °C and 2.5% NaCl. The highest biodegradation rate of strains FM6-1 and FM8-1 was found at 150 mg/L PHE and 200 mg/L, respectively. In addition, strainsFM8-1 showed a superior biodegradation ability to strain FM6-1 at each optimized condition. The PHE biodegradation process by both strains well fitted to first-order kinetic models and the k1 values were calculated to be 0.1974 and 0.1070 per day. Strain FM6-1 metabolized PHE via a "phthalic acid" route, while strain FM8-1 metabolized PHE through the "naphthalene" route. This project not only obtained two halotolerant petroleum hydrocarbon degraders but also provided a promising remediation approach for solving oil pollutants in salinity environments.

Petroleum Hydrocarbon-Degrading Bacteria for the Remediation of Oil Pollution Under Aerobic Conditions: A Perspective Analysis.

With the sharp increase in population and modernization of society, environmental pollution resulting from petroleum hydrocarbons has increased, resulting in an urgent need for remediation. Petroleum hydrocarbon-degrading bacteria are ubiquitous in nature and can utilize these compounds as sources of carbon and energy. Bacteria displaying such capabilities are often exploited for the bioremediation of petroleum oil-contaminated environments. Recently, microbial remediation technology has developed rapidly and achieved major gains. However, this technology is not omnipotent. It is affected by many environmental factors that hinder its practical application, limiting the large-scale application of the technology. This paper provides an overview of the recent literature referring to the usage of bacteria as biodegraders, discusses barriers regarding the implementation of this microbial technology, and provides suggestions for further developments.

[Synthesis and characterization of phenyl-acetylmethylene sulfoxide complexes with light rare earth and fluorescence spectrum of Eu(III) complex].

Five solid complexes of phenyl-acetylmethylene sulfoxide(L) with light rare earth (La, Pr, Nd, Sm, Eu) were synthesized and characterized. Elemental analysis and molar conductivities show that the complexes have a composition of RE(ClO4)3 x L5 x 2H2O; two ClO4 anions coordinated with rare earth ions and the other did not. IR spectra suggested that the ligand coordinated with RE3+ by oxygen atom in sulfinyl group, and the oxygen atom in carbonyl group did not bond. The phosphorescence and 1H NMR and TG-DTA of the ligand and complexes were determind. Fluorescence spectra of Eu(III) complex with the sulfoxide indicated that the complex has no symmetrical center. All the complexes have good solubility.

[Synthesis and luminescence of methyl-phenacyl sulfoxide complexes with heavy rare earth perchlorate].

RE(ClO4)3 x L5 x C2H5OH(RE=Gd, Tb, Dy, Tm, Yb; L = C6H5COCH2SOCH3) complexes were synthesized. The composion of the complexes was determined by elemental analysis, coordination titration, molar conductivities, and TGA studies. The ligand and coordination compounds were studied by means of IR spectra, 1H NMR, and the Tb(II) coordination compound was studied by means of phosphorescence spectra, and fluorescence excitation and emission spectra. Each energy level of Tb3+ was calculated according to the fluorescent emission spectra results.