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1.
BMC Genet ; 18(1): 86, 2017 10 11.
Artigo em Inglês | MEDLINE | ID: mdl-29020918

RESUMO

BACKGROUND: Allotetraploid F1 hybrids (4nF1) (AABB, 4n = 148) were generated from the distant hybridization of Carassius auratus red var. (RCC) (AA, 2n = 100) (♀) × Megalobrama amblycephala (BSB) (BB, 2n = 48) (♂). It has been reported that Hox gene clusters are highly conserved among plants and vertebrates. In this study, we investigated the genomic organization of Hox gene clusters in the allotetraploid F1 hybrids and their parents to investigate the polyploidization process. RESULTS: There were three copies of Hox genes in the 4nF1 hybrids, two copies in RCC and one copy in BSB. In addition, obvious variation and pseudogenization were observed in some Hox genes from 4nF1. CONCLUSION: Our results reveal the influence of polyploidization on the organization and evolution of Hox gene clusters in fish and also clarify some aspects of vertebrate genome evolution.


Assuntos
Genes Homeobox/fisiologia , Variação Genética , Carpa Dourada/genética , Tetraploidia , Animais , Feminino , Carpa Dourada/classificação , Hibridização Genética , Cariotipagem , Masculino , Filogenia , Análise de Sequência de DNA/métodos
2.
Curr Mol Med ; 14(10): 1322-30, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25470285

RESUMO

Diploid natural gynogenetic goldfish (2nGRCG), triploid hybrids (3nRB) and tetraploid hybrids (4nRB) are generated by distant hybridization of red common goldfish (RCG, Carassius auratus red var.) and blunt snout bream (BSB, Megalobrama amblycephala). In the present study, we obtained the complete mitochondrial DNA (mtDNA) sequences of the hybrid offspring and compared them with the homologous sequences of RCG and BSB. All mtDNA sequences of these hybrids were 16,580bp in length, and the genes number, size, and order were quite similar to that of RCG. Genetic analysis revealed that the mtDNA sequences of these hybrids had high similarity (>99%) and low divergence (<2%) to their maternal RCG, yet lower similarities (84%) and higher divergences (16%) to their paternal BSB. The phylogenetic analysis also showed that the sequences of 2nGRCG, 3nRB and 4nRB were clustered with RCG rather than with BSB. These results indicate that the mitochondrial genomes of 2nGRCG, 3nRB and 4nRB remain maternally inherited after hybridization and polyploidization. Moreover, clade separation of hybrid offspring from their paternal BSB in the phylogenetic tree implies that phylogenetic analysis of mtDNA is incomplete for elucidating the true relationships between different species, particularly when they have undergone hybridization or allopolyploidization. Our study provides significant information for both evolution and genetic studies of mtDNA for hybrid species and allopolyploidization species.


Assuntos
Núcleo Celular/genética , Cyprinidae/genética , Genes Mitocondriais , Genoma Mitocondrial , Carpa Dourada/genética , Mitocôndrias/genética , Animais , Evolução Biológica , Quimera , Cyprinidae/classificação , Feminino , Tamanho do Genoma , Carpa Dourada/classificação , Hibridização Genética , Padrões de Herança , Masculino , Filogenia , Ploidias
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