RESUMO
T lymphocyte hyperactivity and progressive inflammation in systemic lupus erythematosus (SLE) patients results in over-expression of human leucocyte antigen (HLA)-Ib on the surface of lymphocytes. These are shed into the circulation upon inflammation, and may augment production of antibodies promoting pathogenicity of the disease. The objective was to evaluate the association of HLA-Ib (HLA-E, HLA-F and HLA-G) antibodies to the disease activity of SLE. The immunoglobulin (Ig)G/IgM reactivity to HLA-Ib and ß2m in the sera of 69 German, 29 Mexican female SLE patients and 17 German female controls was measured by multiplex Luminex(®)-based flow cytometry. The values were expressed as mean flourescence intensity (MFI). Only the German SLE cohort was analysed in relation to the clinical disease activity. In the controls, anti-HLA-G IgG predominated over other HLA-Ib antibodies, whereas SLE patients had a preponderance of anti-HLA-F IgG over the other HLA-Ib antibodies. The disease activity index, Systemic Lupus Erythematosus Disease Activity Index (SLEDAI)-2000, was reflected only in the levels of anti-HLA-F IgG. Anti-HLA-F IgG with MFI level of 500-1999 was associated with active SLE, whereas inactive SLE revealed higher MFI (>2000). When anti-HLA-F IgG were cross-reactive with other HLA-Ib alleles, their reactivity was reflected in the levels of anti-HLA-E and -G IgG. The prevalence of HLA-F-monospecific antibodies in SLE patients was also associated with the clinical disease activity. Anti-HLA-F IgG is possibly involved in the clearance of HLA-F shed from lymphocytes and inflamed tissues to lessen the disease's severity, and thus emerges as a beneficial immune biomarker. Therefore, anti-HLA-Ib IgG should be considered as a biomarker in standard SLE diagnostics.
Assuntos
Autoanticorpos/sangue , Autoanticorpos/imunologia , Antígenos HLA-G/imunologia , Antígenos de Histocompatibilidade Classe I/imunologia , Lúpus Eritematoso Sistêmico/imunologia , Adulto , Idoso , Anticorpos Anti-Idiotípicos/sangue , Biomarcadores/sangue , Feminino , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Lúpus Eritematoso Sistêmico/diagnóstico , Lúpus Eritematoso Sistêmico/fisiopatologia , Masculino , Pessoa de Meia-Idade , Exacerbação dos Sintomas , Adulto Jovem , Antígenos HLA-ERESUMO
Intravenous immunoglobulin (IVIg) reacted with a wide array of human leucocyte antigen (HLA) alleles, in contrast to normal sera, due possibly to the purification of IgG from the pooled plasma. The reactivity of IgG purified from normal sera was compared with that of native sera to determine whether any serum factors mask the HLA reactivity of anti-HLA IgG and whether IgG purified from sera can recognize the HLA types of the corresponding donors. The purified IgG, unlike native sera, mirrored IVIg reactivity to a wide array of HLA-I/-II alleles, indicating that anti-HLA IgG may be masked in normal sera - either by peptides derived from soluble HLA or by those from antibodies. A < 3 kDa peptide from the complementarity-determining region (CDR) of the Fab region of IgG (but not the HLA peptides) masked HLA recognition by the purified IgG. Most importantly, some of the anti-HLA IgG purified from normal sera - and serum IgG from a few donors - indeed recognized the HLA types of the corresponding donors, confirming the presence of auto-HLA antibodies. Comparison of HLA types with the profile of HLA antibodies showed auto-HLA IgG to the donors' HLA antigens in this order of frequency: DPA (80%), DQA (71%), DRB345 (67%), DQB (57%), Cw (50%), DBP (43%), DRB1 (21%), A (14%) and B (7%). The auto-HLA antibodies, when unmasked in vivo, may perform immunoregulatory functions similar to those of therapeutic preparations of IVIg.
Assuntos
Autoanticorpos/imunologia , Regiões Determinantes de Complementaridade/imunologia , Antígenos HLA/imunologia , Fragmentos Fab das Imunoglobulinas/imunologia , Imunoglobulina G/imunologia , Imunoglobulinas Intravenosas/administração & dosagem , Fatores Imunológicos/administração & dosagem , Alelos , Especificidade de Anticorpos , Autoanticorpos/isolamento & purificação , Regiões Determinantes de Complementaridade/isolamento & purificação , Humanos , Fragmentos Fab das Imunoglobulinas/isolamento & purificação , Imunoglobulina G/isolamento & purificação , Imunoglobulinas Intravenosas/imunologia , Fatores Imunológicos/imunologia , MasculinoRESUMO
Activated CD4(+) T cells undergo blastogenesis and proliferation and they express several surface receptors, including ß2-microglobulin-free human leucocyte antigen (HLA) heavy chains (open conformers). Intravenous immunoglobulin (IVIg) suppresses activated T cells, but the mechanism is unclear. IVIg reacts with HLA-Ia/Ib antigens but its reactivity is lost when the anti-HLA-E Ab is adsorbed out. Anti-HLA-E antibodies may bind to the peptides shared by HLA-E and the HLA-I alleles. These shared peptides are cryptic in intact HLA, but exposed in open conformers. The hypothesis that anti-HLA-E monoclonal antibodies (mAbs) that mimic HLA-I reactivity of IVIg may suppress activated T cells by binding to the shared peptides of the open conformers on the T cell surface was tested by examining the relative binding affinity of those mAbs for open conformers coated on regular beads and for intact HLA coated on iBeads, and by comparing the effects on the suppression of phytohaemagglutinin (PHA)-activated T cells of three entities: IVIg, anti-HLA-E mAbs that mimic IVIg [Terasaki Foundation Laboratory (TFL)-006 and (TFL)-007]; and anti-HLA-E antibodies that do not mimic IVIg (TFL-033 and TFL-037). Suppression of blastogenesis and proliferation of those T cells by both IVIg and the anti-HLA-E mAbs was dose-dependent, the dose required with mAbs 50-150-fold lower than with IVIg. TFL-006 and TFL-007 significantly suppressed blastogenesis and proliferation of activated CD4(+) T cells, but neither the non-IVIg-mimicking mAbs nor control antibodies did so. The suppression may be mediated by Fab-binding of TFL-006/TFL-007 to the exposed shared peptides. The mAb binding to the open conformer may signal T cell deactivation because the open conformers have an elongated cytoplasmic tail with phosphorylation sites (tryosine(320)/serine(335)).
Assuntos
Anticorpos Monoclonais/imunologia , Linfócitos T CD4-Positivos/imunologia , Antígenos HLA/imunologia , Imunoglobulinas Intravenosas/imunologia , Ativação Linfocitária/imunologia , Processos de Crescimento Celular/imunologia , Antígenos de Histocompatibilidade Classe I/imunologia , Humanos , Masculino , Peptídeos/imunologia , Antígenos HLA-ERESUMO
B memory cells remain in circulation and secrete alloantibodies without antigen exposure > 20 years after alloimmunization postpartum or by transplantation. These long-lived B cells are resistant to cytostatic drugs. Therapeutically, intravenous immunoglobulin (IVIg) is administered to reduce allo-human leucocyte antigen (HLA) antibodies pre- and post-transplantation, but the mechanism of reduction remains unclear. Recently, we reported that IVIg reacts with several HLA-I alleles and the HLA reactivity of IVIg is lost after its HLA-E reactivity is adsorbed out. Therefore, we have generated an anti-HLA-E monoclonal antibody that mimics the HLA-reactivity of IVIg to investigate whether this antibody suppresses IgG secretion, as does IVIg. B cells were purified from the blood of a woman in whose blood the B memory cells remained without antigen exposure > 20 years after postpartum alloimmunization. The B cells were stimulated with cytokines using a well-defined culture system. The anti-HLA-E monoclonal antibody (mAb) significantly suppressed the allo-HLA class-II IgG produced by the B cells, and that this suppression was far superior to that by IVIg. These findings were confirmed with HLA-I antibody secreted by the immortalized B cell line, developed from the blood of another alloimmunized woman. The binding affinity of the anti-HLA-E mAb for peptide sequences shared (i.e. shared epitopes) between HLA-E and other ß2-microglobulin-free HLA heavy chains (open conformers) on the cell surface of B cells may act as a ligand and signal suppression of IgG production of activated B memory cells. We propose that anti-HLA-E monoclonal antibody may also be useful to suppress allo-HLA IgG production in vivo.
Assuntos
Formação de Anticorpos/imunologia , Linfócitos B/imunologia , Antígenos HLA/imunologia , Memória Imunológica , Isoanticorpos/imunologia , Adulto , Alelos , Sequência de Aminoácidos , Animais , Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/farmacologia , Formação de Anticorpos/efeitos dos fármacos , Linfócitos B/efeitos dos fármacos , Linfócitos B/metabolismo , Células Cultivadas , Criança , Epitopos de Linfócito B/química , Epitopos de Linfócito B/imunologia , Feminino , Antígenos HLA/química , Antígenos HLA/genética , Cadeias beta de HLA-DR/genética , Cadeias beta de HLA-DR/imunologia , Antígenos de Histocompatibilidade Classe I/química , Antígenos de Histocompatibilidade Classe I/genética , Antígenos de Histocompatibilidade Classe I/imunologia , Humanos , Imunização , Imunoglobulina G/imunologia , Imunoglobulina G/farmacologia , Imunoglobulinas Intravenosas/imunologia , Imunoglobulinas Intravenosas/farmacologia , Memória Imunológica/efeitos dos fármacos , Imunofenotipagem , Ativação Linfocitária/imunologia , Masculino , Camundongos , Pessoa de Meia-Idade , Dados de Sequência Molecular , Fenótipo , Adulto Jovem , Antígenos HLA-ERESUMO
GD3 is a major ganglioside of human melanoma and was shown to be an effective target for passive immunotherapy with murine monoclonal antibodies. It was noted earlier that GD3 neither purified nor in melanoma cell vaccine (MCV), could elicit an antibody response in melanoma patients. In this study, we demonstrate that melanoma patients who received MCV had autoantibodies against a derivative of GD3, O-acetylated GD3 (O-AcGD3), a minor ganglioside expressed on human melanoma cells, and that the antibodies cross-reacted with GD3. Thin layer chromatographic immunostaining revealed that all of the sera containing antibodies against O-AcGD3 also reacted to GD3. None of the other sera responded only to GD3, although the MCV contained 7- to 12-fold higher GD3 than O-AcGD3. Furthermore, the antibody activity was completely abolished by absorption with animal erythrocytes expressing either O-acetyl disialogangliosides or GD3, indicating that the antibodies recognize an epitope commonly shared by GD3 and O-AcGD3. The antibodies bound only to the sialyloligosaccharide moiety but not to the ceramide portion of GD3 after endoglycosylceramidase treatment. The antibodies failed to bind to GD3 after neuraminidase treatment. These results indicate that the sialyloligosaccharides of the gangliosides are important components of the epitope. Periodate oxidation abolished reactivity of the antibodies to GD3 but not that to O-AcGD3, revealing that the glycerol side chain of the sialic acids in both GD3s was an important structure of the epitope. The binding of the antibodies to melanoma cell surface gangliosides was confirmed by an absorption with a GD3- and O-AcGD3-positive melanoma cell line. These results in the light of previous reports on the inability of GD3 to elicit immune response in humans suggest that anti-GD3 antibodies found in the melanoma patients were induced by immunization with O-AcGD3 and O-AcGD3 present in the MCV would serve as an antigen source for GD3-targeted active specific immunotherapy of melanoma.
Assuntos
Antígenos de Neoplasias/análise , Epitopos/análise , Gangliosídeos/análise , Imunoterapia , Melanoma/imunologia , Anticorpos , Especificidade de Anticorpos , Antígenos de Neoplasias/imunologia , Ensaio de Imunoadsorção Enzimática , Imunofluorescência , Gangliosídeos/imunologia , Humanos , Melanoma/terapia , Vacinas/administração & dosagemRESUMO
A human B-lymphoblastoid cell clone, L55-81, that produces human monoclonal antibody (MAb) to ganglioside G(M2) was established from peripheral blood B lymphocytes of a melanoma patient. L55-81 secretes IgMkappa light chain antibody in a serum-free medium. G(M2) specificity of the antibody was tested by immune adherence assay, TLC immunostaining, and ELISA. Anti-G(M2) antibody was shown to have the ability to kill the G(M2)-rich human melanoma cell line M14 in the presence of human or rabbit complement. A purified L55-81 MAb (>99.5% purity in protein concentration) was biotinylated and tested for its reactivity to various histological-type biopsied tumor and normal tissues in an avidin-biotin detection system. L55-81 MAb (20 microg/ml) reacted with several types of tumor tissues such as melanoma (7 of 10), colon carcinoma (4 of 5), ovary carcinoma (4 of 5), breast carcinoma (1 of 5), kidney carcinoma (1 of 5), and prostate carcinoma (1 of 5). None of the normal tissues derived from 24 different organs and adjacent normal tissues surrounding the cancerous tissues were stained. Production of the antibody in a serum-free medium, the cytotoxic potential with human complement, the inability to react to normal tissues, and the ability to target antigen-specific target cells make L55-81 a potential therapeutic agent for the treatment of cancers expressing ganglioside G(M2).
Assuntos
Anticorpos Monoclonais/imunologia , Especificidade de Anticorpos , Linfócitos B/imunologia , Gangliosídeo G(M2)/imunologia , Melanoma/terapia , Anticorpos Monoclonais/metabolismo , Linfócitos B/metabolismo , Linhagem Celular Transformada , Epitopos/imunologia , Gangliosídeo G(M2)/metabolismo , Humanos , Imunoterapia , Melanoma/imunologia , Células Tumorais CultivadasRESUMO
The ganglioside composition of human melanoma was analyzed in five sets of tumor specimens obtained directly from surgery, from the autologous tissue culture cell lines, and from the autologous cell lines grown in athymic nude mice. Total gangliosides of these 15 melanoma specimens were isolated and purified, and the amount of each component ganglioside was analyzed by thin-layer chromatography and a thin-layer chromatography scanner. The ganglioside composition of the five surgical melanoma specimens clearly exhibited different patterns from each other. Moreover, none of the autologous cultured melanomas possessed the same ganglioside composition as their original biopsied tumors. However, when these melanoma cell lines were transplanted into nude mice, the ganglioside composition was converted back to the same ganglioside pattern as in the original surgical specimens. The results support the view that changes in the ganglioside composition of melanoma during in vitro growth are caused by the culture environment rather than by selection of melanoma cells with a particular genotype. Reestablishment of the original ganglioside patterns after passage in nude mice provides clear evidence that in vivo expression of gangliosides is a conserved and stable function specified by the human melanoma cells.
Assuntos
Gangliosídeos/análise , Melanoma/análise , Animais , Linhagem Celular , Humanos , Masculino , Camundongos , Metástase Neoplásica , Transplante de Neoplasias , Transplante HeterólogoRESUMO
The major problem associated with ELISA of serum antiganglioside antibodies is the high background values (absorbancy of sera added to wells without ganglioside), which interfere with the accurate assessment of the fine specificity and sensitivity of these antibodies. This investigation identifies factors elevating the background values and/or decreasing the fine specificity, and describes strategies to minimize their influence. Using sera of neuropathy and melanoma patients, we found that highest background values were observed with the polystyrene 'tissue culture' microtiter plates; of the various 'non-tissue culture' microtiter plates tested, the lowest background values (> 0.060) were observed with Costar-3590 (H), Immunolon-3, Immunolon-1, Falcon-3915 (in increasing order). Background artifact of polystyrene microtest plates was significantly reduced by gamma irradiation (at 40 kRad) and/or use of detergent Tween-20 (0.1%) in the washing step. Even after controlling the background values, the fine specificity, namely, the ability of the antibody to distinguish between the target epitope of an antigen and epitopes of related antigens (when moles of antigen/well is constant) varied with different microtiter plates. Using sera with high affinity and specificity for GM2, GD3 or GM3, we observed that Immunolon-1, Immunolon-3 and particularly Falcon-3915 were superior for assessing the abilities of the antibodies to distinguish closely related epitopes found on other gangliosides. The reactivity of antiganglioside antibodies was more consistent after detergent treatment. The reactivity of antibodies to GD3 is significantly enhanced after treatment with Tween-20, but that of antibodies reacting to GM1 and GM2 is reduced. Fine specificity of the antiglycolipid antibodies was resolved better by coating glycolipids in mol/well rather than by weight/well. Based on these results, a protocol for a sensitive and reproducible ELISA for serum antiganglioside antibodies is recommended. The protocol takes into consideration the suitability of polystyrene plates, coating based on the number of molecules, pertinency of the solvent for coating, use of human serum albumin for blocking, dilution and washing steps and use of 0.1% Tween-20 to further minimize the background absorbancy.
Assuntos
Autoanticorpos/análise , Ensaio de Imunoadsorção Enzimática/métodos , Gangliosídeos/imunologia , Sequência de Carboidratos , Epitopos/imunologia , Humanos , Imunoglobulina G/imunologia , Imunoglobulina M/imunologia , Técnicas Imunológicas/instrumentação , Melanoma/imunologia , Dados de Sequência Molecular , Doenças do Sistema Nervoso/imunologia , Polissorbatos , Reprodutibilidade dos Testes , Sensibilidade e EspecificidadeRESUMO
The density of carbohydrate epitopes on the surface of tumor cells is a governing factor for immune recognition and antibody-mediated targeting of tumor-associated carbohydrate antigens in cancer immunotherapy. A sensitive cell-suspension ELISA (cs-ELISA) is developed for quantitation of the functionally exposed carbohydrate epitopes on the cell surface. The factors affecting the measurement of tumor-cell surface glycoconjugates are evaluated using three human melanoma cell lines before and after exposure to various cell preservation treatments. The results of cs-ELISA are compared with the quantitative profile obtained by biochemical and flow cytometry assays. Cs-ELISA measures the density of the functionally exposed specific sugar epitopes on the surface of tumor cells, even in the presence of other similar carbohydrate antigens, provided that the monoclonal antibodies to carbohydrate epitopes are monospecific and sensitive, and that the cells are viable and present in optimal density. Of the three melanoma cell lines, M10-v and M101 expressed disialolactosyl residues of GD3 at concentrations of 5-6 pmol/10(6) cells and 2-3 pmol/10(6) cells, respectively. In both cell lines, the cell-surface GD2 was less than 1.0 pmol/10(6) cells. M24 melanoma cells expressed trace quantities (< 0.1 pmol/10(6) cells) of GD3 and GD2. Trypsinization of M10-v and M101 cells significantly reduced the cell-surface expression of GD3, suggesting GD3 loss, but increased the expression of GD2, suggesting crypticity of membrane-bound GD2. Cs-ELISA results showed that cryopreservation with 10% DMSO and irradiation at 15 krad decreased melanoma cell viability and ganglioside expression for M10-v but not M101 and M24. Formalinization did not affect cs-ELISA measurement of cell-surface carbohydrates. Cs-ELISA was used to monitor the quantity of incorporation of exogenous GD3 onto the surface of GD3-deficient M24 cells. Cs-ELISA for assessment of density of cell surface carbohydrate epitopes may be useful to characterize different types of tumors, to develop carbohydrate-based whole cell vaccines from tumor biopsies, to monitor the effects of cell preservation treatments commonly used in a whole cell vaccine preparation, and to evaluate the incorporation of a particular glycolipid (antigen or adjuvant) into glycolipid-deficient cells that are useful for carbohydrate-based active specific immunotherapy.
Assuntos
Antígenos de Superfície/análise , Antígenos Glicosídicos Associados a Tumores/análise , Melanoma/imunologia , Neoplasias Cutâneas/imunologia , Anticorpos Monoclonais/imunologia , Anticorpos Antineoplásicos/imunologia , Especificidade de Anticorpos , Reações Antígeno-Anticorpo , Sequência de Carboidratos , Ensaio de Imunoadsorção Enzimática/métodos , Congelamento , Gangliosídeos/análise , Humanos , Dados de Sequência Molecular , Preservação Biológica , Tripsina/farmacologia , Células Tumorais CultivadasRESUMO
The Defense reactions of Scylla serrata have been investigated by challenging crabs with two metalloproteins, horse radish peroxidase (HRP:M.W. 40,000) and hemoglobin (Hb:64,000). The defense response analyzed include 1) recognition of foreign proteins; 2) clearance from the haemocoel; 3) accumulation in tissues and 4) externalization. Protein clearance was similar to dye clearance in several respects: 1) clearance rate increased with higher a) molecular weight and concentration and b) at night and 2) decreased with crab size. HRP clearance rate was significantly increased after repeated injections of HRP but not saline. Clearance was not enhanced after repeated injections of Hb. About 70% of HRP accumulates in gills within 2 hours after injection, when there were no signs of HRP accumulation in the hepatopancreas. But about the same percent of injected Hb accumulated in both gills and hepatopancreas after 2 hours of injection. When 300 ug HRP declined in gills after 8 hour, similar amounts of HRP accumulated in the hepatopancreas. Moreover when 325 ug of Hb was lost from gills after 4 hours, the hepatopancreas accumulated about 300 ug, suggesting that foreign proteins may undergo antigenic alteration in the gills prior to mobilization to the hepatopancreas. There is no evidence of degradation of proteins in gills. When HRP and Hb level declined in the hepatopancreas, the levels of these proteins increased in gut contents indicating externalization of injected proteins via gut.
Assuntos
Braquiúros/metabolismo , Proteínas/metabolismo , Animais , Braquiúros/imunologia , Brânquias/metabolismo , Hemoglobinas/metabolismo , Hemolinfa/metabolismo , Peroxidase do Rábano Silvestre/metabolismo , Fígado/metabolismo , Pâncreas/metabolismo , Proteínas/imunologiaRESUMO
Clearance rates of dyes injected into the hemocoel of the mud crab, Scylla serrata, revealed several patterns of clearance and retention associated with physico-chemical and biological variables. Clearance rates remained constant: 1) when the molar concentrations of the injected dyes were equal, 2) after repeated injections of dyes and 3) after serum opsonization. Rates increased: 1) with higher molecular weight irrespective of charge, 2) at higher dye concentration and 3) at night concomitant with an increase in the hemocyte population. In contrast, clearance rates decreased with increasing crab size. Dye cleared from the hemolymph accumulated in the gills but not in the hepatopancreas, antennary glands nor gut. The retention of dyes in the gills increased with higher molecular weights. Granular hemocytes accumulated in the gill rachis and lamellae of dye treated but not in gills of normal and saline injected crabs. A role for hemocytes in molecular weight and concentration dependent clearance of dyes is suggested. Our findings are important for understanding the molecular basis of recognition in crustaceans.
Assuntos
Braquiúros/fisiologia , Corantes/metabolismo , Animais , Braquiúros/metabolismo , Corantes/administração & dosagem , Brânquias/análise , Hemolinfa/metabolismo , Taxa de Depuração Metabólica , Peso Molecular , Distribuição TecidualRESUMO
Among various tumor associated cell surface antigens, gangliosides, the glycosphingolipids that contain sialic acids, offer a variety of epitopes, some of which are preferentially expressed on melanoma cells. These surface components of the bilayered lipid membrane of tumor cells are the targets of active immunotherapy with melanoma vaccine. Purified gangliosides in aqueous solution form micelles and, at high density, form lactones. Their antigenic expression (physical conformation and orientation) on the cell surface is governed by the nature of the sphingosine and the fatty acids they contain. Evidence is accruing to show that the nature of the fatty acid moiety of gangliosides differs in normal and neoplastic cells. Gangliosides per se are not immunogenic and require extrinsic adjuvanticity. Preparation of a melanoma cell vaccine for active immunotherapy requires an understanding of the ganglioside profile of melanoma, the ganglioside-associated heterogeneity of melanoma, and the role of shed melanoma gangliosides in the immunosuppression of cell mediated and humoral immunity. In addition, the role of some of the anti-ganglioside antibodies in the elimination of shed gangliosides, the cytotoxic killing of tumor cells, as well as in the down-regulation of lymphocyte functions must be considered in the formulation of vaccine. Different strategies for augmenting the immunogenicity of melanoma associated gangliosides with melanoma vaccine are evaluated.
Assuntos
Gangliosídeos/imunologia , Imunoterapia Ativa , Melanoma/imunologia , Melanoma/terapia , Vacinas , Ensaios Clínicos como Assunto , Gangliosídeos/química , Humanos , Terapia de Imunossupressão , Imunoterapia Adotiva , Melanoma/químicaRESUMO
BACKGROUND: Several investigators have proposed that carcinoembryonic antigen (CEA), an immunogenic antigen expressed by colon carcinoma, may also be expressed by human melanoma. Because sialyl Lewisx (sLex), the carbohydrate moiety of CEA, has been identified in melanoma, we compared CEA and sLex levels in colon carcinoma cells and melanoma cells. METHODS: CEA levels were assessed for expression on the cell surface and in cell lysates of cutaneous melanoma cell lines by two different kinds of ELISA, and by Western blot analysis of immunoprecipitated CEA using monoclonal antibodies (Mabs) T84-66 and COL-1, which have defined specificities for CEA. Colon carcinoma cells and purified CEA were positive controls. RESULTS: Both Mabs reacted strongly with cell surface and cell lysates of colon cancer. Mab T84-66 reacted well with cell surface but not cell lysates of melanoma. COL-1 reacted poorly with cell surface but its binding increased with the density of melanoma cell lysates. Both Mabs intensely stained the blots of purified CEA and colon carcinoma lysates immunoprecipitated with the respective Mabs, but failed to stain the immunoprecipitates of melanoma cell lysates. Both Mabs bound to lysates immunoprecipitated with anti-sLex Mab in colon carcinoma, but not in melanoma. Cell-surface expression of CEA and sLex was significantly correlated (r2: 0.88) in colon cancer cells but not in melanoma. CONCLUSION: Our results confirm the presence of CEA in colon carcinoma but not in human cutaneous melanoma cell lines.
Assuntos
Antígeno Carcinoembrionário/biossíntese , Melanoma/imunologia , Neoplasias Cutâneas/imunologia , Animais , Western Blotting/métodos , Neoplasias do Colo/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Humanos , Camundongos , Oligossacarídeos/metabolismo , Testes de Precipitina/métodos , Antígeno Sialil Lewis X , Células Tumorais CultivadasRESUMO
A simple procedure is deviced to detect chitin in egg shells and integument of parasites using iodinesulfuric acid method. It is observed that the materials do not survive in saturated alkali at 160 degrees C for 15 min, but resist the treatment of 40% KOH at 90 degrees C for 25 min, indicating that the time and temperature required for optimum hydrolysis may differ from material to material. In some preparations, an yellow-green colour was observed, which was occluded and a positive reaction was obtained with acid pretreatment, indicating that incomplete neutralization may prevent the reaction of chitin aggregates with iodine.
Assuntos
Quitina/análise , Álcalis , Animais , Histocitoquímica , Hidrólise , Métodos , Temperatura , Fatores de TempoRESUMO
The tridentate pedicellaria of Salmacis bicolor consists of calcareous head and stalk, connected by a muscular neck regions. Head, neck and stalk are covered by a thin cuticle, which is non-mineralized and soft. Both the cuticle and the organic fraction of the calcareous region are resistant to acids and alkalis. Histochemical studies reveal that both the cuticle and the organic matrix of the calcified head and stalk contain protein, polysaccharide and lipids. The lipid content of the organic fraction of the mineralized matrix recalls the spicules of Pluteus larva reported in an earlier study. The protein component of the cuticle and that of the organic matrix slightly differ in that the former is rich in aromatic amino acids, namely tyrosine and tryptophane. Both cuticle protein and the protein of the organic matrix lack sulphur containing aminoacids. Another feature of similarity between the cuticle and the organic matrix is that both contain acid mucopolysaccharide and neutral mucopolysaccharide components. The role of acid mucopolysaccharide in calcification is discussed. The neutral mucopolysaccharide has been suggested to be similar to chitin, and its significance is discussed. Unlike the skeletal elements of other invertebrates, the cuticle and the organic matrix of pedicellariae are devoid of any phenolic compounds.
Assuntos
Ouriços-do-Mar/análise , Aminoácidos/análise , Aminoácidos Sulfúricos/análise , Calcificação Fisiológica , Quitina/análise , Glicosaminoglicanos/análise , Histocitoquímica , Humanos , Lipídeos/análise , Fenóis/análise , Proteínas/análise , Pele/análiseRESUMO
This review discusses the immunology of gangliosides from the perspective of tumor, neuronal and general immunology. Antiganglioside antibodies in human sera are invariably IgM and are found in healthy individuals. Their titers decline with age. Persistent high titer of IgM is associated with several diseases, particularly neuropathies. Membrane-bound gangliosides are important tumor-associated antigens and targets for immune attack. Cells enriched with gangliosides can be used as cancer vaccines. Efficacy of these vaccines depends on the viability of whole cells, integrity of the cell membranes, adjuvants and topography of the tumor-associated antigens. The role of antiganglioside IgM is to eliminate the immunosuppressive gangliosides shed from tissues during ageing, degeneration of neural and extraneural tissues, and tumor growth and necrosis. In addition, in vitro observations with human and murine monoclonal antibodies suggest that they are capable of complement dependent cytotoxicity and apoptosis.