[Conventional epiphyseal chondrosarcoma of childhood and adolescence: a case report]. / Konventionelles epiphysäres Chondrosarkom des Kindes- und Jugendalters: ein Fallbericht.

INTRODUCTION: Conventional chondrosarcoma is the second most common primary malignant bone tumor and usually occurs at older adult ages. It is rare in childhood and adolescence. CASE HISTORY: This case report presents the treatment course of a 13-year-old boy with a symptomatic chondrogenic tumor of the right distal femur. Histopathologically, an epiphyseal intermediate-grade chondrosarcoma (G2) was diagnosed. DISCUSSION: Based on the following case, potential radiological and histopathological differential diagnoses, such as chondroblastoma or chondroblastic osteosarcoma, are discussed against the background of current standards in orthopedic oncology.

Acceptable mid- to long-term survival rates and functional outcomes following a single design rotating hinge total knee arthroplasty.

PURPOSE: This study first analyzes implant survival of this single design modular rotating hinge knee and identifies potential risk factors for failure and evaluates joint function using the postoperative WOMAC (Western Ontario and McMaster Universities Osteoarthritis Index) score, active flexion and extension deficit. METHODS: 131 prostheses implanted for failure of prior total knee arthroplasty (n = 120) or complex primary procedures (n = 11) using a single modular implant (MUTARS-modular universal tumor and revision system GenuX, Implantcast, Buxtehude, Germany) between 2006 and 2014 including 73 patients treated for periprosthetic joint infection with a two-stage revision protocol were retrospectively identified. Implant survival was assessed using the Kaplan-Meier method; potential risk factors were identified using the log-rank test, as well as non-parametric analysis. Postoperative function was assessed using the WOMAC and measurement of range of motion. RESULTS: After a median follow-up of 62 months, 37 implants required implant revision (28%). Five-year survival was 69.7% [95% CI (confidence interval) 60.9-78.5] with periprosthetic (re-) infection being the main cause for failure (15%), followed by aseptic loosening (9%). In cases of periprosthetic infection, infection-free survival was 83% at 5 years (95% CI 74-92) with twelve patients suffering reinfection (16%).While body mass index (p = 0.75), age (p = 0.16) or indication for rotating hinge knee arthroplasty (p = 0.25) had no influence on survival, Charlson comorbidity score (CCI) (p = 0.07) and number of previous revision surgeries (p = 0.05) correlated with implant failure. There was trend (p = 0.1) for improved survival in fully cemented implants. Mean postoperative WOMAC was 127(range 55-191), 11 patients (15%) had limited knee extension. CONCLUSIONS: Rotating hinge total knee arthroplasty using a single modular implant shows acceptable survival rates and function compared to previous studies with (re-)infection being the most relevant mode of failure. Patients with a high CCI and multiple previous surgeries are at increased risk for failure. LEVEL OF EVIDENCE: Retrospective cohort study, III.

Transitive inference in two lemur species (Eulemur macaco and Eulemur fulvus).

When confronted with tasks involving reasoning instead of simple learning through trial and error, lemurs appeared to be less competent than simians. Our study aims to investigate lemurs' capability for transitive inference, a form of deductive reasoning in which the subject deduces logical conclusions from preliminary information. Transitive inference may have an adaptative function, especially in species living in large, complex social groups and is proposed to play a major role in rank estimation and establishment of dominance hierarchies. We proposed to test the capacities of reasoning using transitive inference in two species of lemurs, the brown lemur (Eulemur fulvus) and the black lemur (Eulemur macaco), both living in multimale-multifemale societies. For that purpose, we designed an original setup providing, for the first time in this kind of cognitive task, pictures of conspecifics' faces as stimuli. Subjects were trained to differentiate six photographs of unknown conspecifics named randomly from A to F to establish the order A > B > C > D > E > F and select consistently the highest-ranking photograph in five adjacent pairs AB, BC, CD, DE, and EF. Then lemurs were presented with the same adjacent pairs and three new and non-adjacent pairs BD, BE, CE. The results showed that all subjects correctly selected the highest-ranking photograph in every non-adjacent pair, reflecting lemurs' capacity for transitive inference. Our results are discussed in the context of the still debated current theories about the mechanisms underlying this specific capacity.

Inferences about the location of food in lemurs (Eulemur macaco and Eulemur fulvus): a comparison with apes and monkeys.

The ability of black and brown lemurs (Eulemur macaco and Eulemur fulvus) to make inferences about hidden food was tested using the same paradigm as in Call's (J Comp Psycol 118:232-241, 2004) cup task experiment. When provided with either visual or auditory information about the content of two boxes (one empty, one baited), lemurs performed better in the auditory condition than in the visual one. When provided with visual or auditory information only about the empty box, one subject out of four was above chance in the auditory condition, implying inferential reasoning. No subject was successful in the visual condition. This study reveals that (1) lemurs are capable of inferential reasoning by exclusion and (2) lemurs make better use of auditory than visual information. The results are compared with the performances recorded in apes and monkeys under the same paradigm.

The Magnetic Electron Ion Spectrometer: A Review of On-Orbit Sensor Performance, Data, Operations, and Science.

Measurements from NASA's Van Allen Probes have transformed our understanding of the dynamics of Earth's geomagnetically-trapped, charged particle radiation. The Van Allen Probes were equipped with the Magnetic Electron Ion Spectrometers (MagEIS) that measured energetic and relativistic electrons, along with energetic ions, in the radiation belts. Accurate and routine measurement of these particles was of fundamental importance towards achieving the scientific goals of the mission. We provide a comprehensive review of the MagEIS suite's on-orbit performance, operation, and data products, along with a summary of scientific results. The purpose of this review is to serve as a complement to the MagEIS instrument paper, which was largely completed before flight and thus focused on pre-flight design and performance characteristics. As is the case with all space-borne instrumentation, the anticipated sensor performance was found to be different once on orbit. Our intention is to provide sufficient detail on the MagEIS instruments so that future generations of researchers can understand the subtleties of the sensors, profit from these unique measurements, and continue to unlock the mysteries of the near-Earth space radiation environment. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s11214-021-00855-2.

A Revised Look at Relativistic Electrons in the Earth's Inner Radiation Zone and Slot Region.

We describe a new, more accurate procedure for estimating and removing inner zone background contamination from Van Allen Probes Magnetic Electron Ion Spectrometer (MagEIS) radiation belt measurements. This new procedure is based on the underlying assumption that the primary source of background contamination in the electron measurements at L shells less than three, energetic inner belt protons, is relatively stable. Since a magnetic spectrometer can readily distinguish between foreground electrons and background signals, we are able to exploit the proton stability to construct a model of the background contamination in each MagEIS detector by only considering times when the measurements are known to be background dominated. We demonstrate, for relativistic electron measurements in the inner zone, that the new technique is a significant improvement upon the routine background corrections that are used in the standard MagEIS data processing, which can "overcorrect" and therefore remove real (but small) electron fluxes. As an example, we show that the previously reported 1-MeV injection into the inner zone that occurred in June of 2015 was distributed more broadly in L and persisted in the inner zone longer than suggested by previous estimates. Such differences can have important implications for both scientific studies and spacecraft engineering applications that make use of MagEIS electron data in the inner zone at relativistic energies. We compare these new results with prior work and present more recent observations that also show a 1-MeV electron injection into the inner zone following the September 2017 interplanetary shock passage.

Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide.

The factors underlying the survival and maintenance of interstitial cells of Cajal (ICC) are not well understood. Loss of ICC is often associated with loss of neuronal nitric oxide synthase (nNOS) in humans, suggesting a possible link. The aim of this study was to determine the effect of neuronal NO on ICC in the mouse gastric body. The volumes of ICC were determined in nNOS(-/-) and control mice in the gastric body and in organotypic cultures using immunohistochemistry, laser scanning confocal microscopy and three-dimensional reconstruction. ICC numbers were determined in primary cell cultures after treatment with an NO donor or an NOS inhibitor. The volumes of myenteric c-Kit-immunoreactive networks of ICC from nNOS(-/-) mice were significantly reduced compared with control mice. No significant differences in the volumes of c-Kit-positive ICC were observed in the longitudinal muscle layers. ICC volumes were either decreased or unaltered in the circular muscle layer after normalization for the volume of circular smooth muscle. The number of ICC was increased after incubation with S-nitroso-N-acetylpenicillamine and decreased by N(G)-nitro-l-arginine. Neuronally derived NO modulates ICC numbers and network volume in the mouse gastric body. NO appears to be a survival factor for ICC.

Axl receptor tyrosine kinase expression in human lung cancer cell lines correlates with cellular adhesion.

Axl is a receptor tyrosine kinase (RTK) with oncogenic potential and transforming activity. Since Axl bears structural similarities to cell adhesion molecules such as neural cell adhesion molecule (NCAM) (FNIII domains), it is thought that Axl might play a role in adhesion. In this study, we have analysed the expression of the Axl protein and its ligand, Gas6, in human lung cancer cell lines of different histological origin. Axl expression occurred in approximately 60% of non-small cell lung cancer (NSCLC) cell lines, which grow adherently, and in normal bronchial epithelial cells (NHBE), but not in cell lines of small cell lung cancer origin (SCLC), which grow in suspension. A number of SCLC sublines, which could be selected spontaneously or after oncogene transfection for adherent growth, all expressed Axl protein. Overexpression of Axl per se, however, did not induce any change in the adhesion phenotype. All Axl-expressing cell lines demonstrated a membrane-bound 140 kD form, as well as a soluble 85 kD form, detectable in supernatant, of Axl-RTK. Expression of the Axl ligand Gas6 was detected in approximately 80% of all cell lines investigated. We conclude from these data that loss of Axl expression is a feature of SCLC tumour cells. Axl expression appears to be a consequence of cellular adhesion and possibly influences differentiation in human lung cancers.

Responses to a novel food acquisition task in three species of lemurs.

In order to study learning and social processes in lemurs, a food acquisition task was presented to three groups: Lemur macaco, L. fulvus and L. catta. The lid of a box had to be pivoted horizontally in order to expose figs contained therein. Individuals in all three groups learned the task, but they showed only limited comprehension. In L. macaco, two adult females learned, with one female showing increasing aggressive monopolization of the new food-source. In L. fulvus, two adult males learned, and one of them increasingly prevented the other from approaching. In L. catta, the adult female frequently threatened away the most efficient opener - a young adult male. The simultaneous presentation of two boxes led to a more equal distribution of the figs. Social processes revealed in the test situation included female dominance in L. macaco and L. catta, and an intermale dominance relationship in L. fulvus. The experimental situation also highlighted the privileged position of the offspring of the dominant female in L. macaco, reduced competitiveness of females with very young infants in L. fulvus, and a general tolerance towards others in L. fulvus.

Social processes and innovative behaviour in changing groups of lemur fulvus.

A group of brown lemurs was presented with one or two baited food-boxes requiring a specific type of motor response in order to be opened. Subsequently, four groups containing different combinations of experienced individuals from the original group and naive individuals were tested. Solutions to the problem and access to the food were recorded and considered in relation to social factors. In the original group, two adult males learned to open the boxes, with one male increasingly preventing the other from approaching. In the second group, with the subordinate male and certain females removed, the dominant male tolerated successful performances by a juvenile female. Group 3 consisted of three passive female participants from the original group and a naive female; one of the three original females now became the sole box-opener. The introduction of the subordinate male from the original group into the all-female group led to a sharing of box-opening by this subject and the skilled female. In the final group, intense aggression toward the skilled female by a new, naive adult male resulted in two previously passive females succeeding on some occasions. In lemurs, at least some 'scroungers' appear able to learn to perform a new act when the social context permits.

Monoclonal antibodies directed against tick-borne encephalitis virus with neutralizing activity in vivo.

Monoclonal antibodies (MoAbs) were raised against the tick-borne encephalitis (TBE) virus, strain K23. The reactivities of 14 selected MoAbs were characterized by ELISA, Western blot analysis, haemagglutination inhibition, immunoprecipitation, in vivo protection and in vitro neutralization tests. All MoAbs reacted only with the glycoprotein E. The binding epitope of one MoAb could be delimited by a synthetic peptide to amino acids 306-339 representing one immunodominant loop structure of the glycoprotein E. The MoAbs exhibited individual reactivities against 13 different TBE virus isolates in ELISA and immunoblot test ranging from type-specific reactions to a broad reactivity with all isolates. Four MoAbs also showed a cross-reaction with other flaviviruses like West Nile virus and/or Yellow fever virus in immunoblot analysis. By competition ELISA the MoAbs could be divided into five different reaction patterns. Four MoAbs showed neutralizing activity with titers in the range 1:140 to 1:5,000 in an in vitro assay. These neutralizing activities could be confirmed by an in vivo mouse challenge model. The MoAbs are useful for diagnostic purposes and for differentiation of TBE virus strains and other flaviviruses.

Tee shirt management of nursemaid's elbow.

Subluxation of the radial head, or "nursemaid's elbow," is a common injury among children aged 1 to 4 years. The authors present a technique for flexed-elbow stabilization designed especially for very young children, which provides adequate immobilization without the use of a sling.

Zeólitas naturais na dieta de frangos de corte / Natural zeolites in broiler diet

O objetivo deste trabalho foi avaliar o efeito da inclusão de 0,5% da zeólita natural clinoptilolita na dieta de frangos de corte sobre o consumo de água, ração e características das excretas. Foi conduzido um ensaio em gaiolas metabólicas, com frangos de corte de linhagem comercial, no período de 14 a 23 dias de idade. O experimento foi constituído de dois grupos de aves, sendo que um recebeu dieta sem inclusão de zeólitas (controle) e outro dieta com inclusão de 0,5% de zeólita (clinoptilolita) em rações isonutritivas, em um delineamento inteiramente ao acaso, com 10 repetições de 10 aves. Avaliou-se o consumo de água e de ração, nas excretas, pH, teor de nitrogênio total e umidade. A inclusão de 0,5% de zeólitas naturais na dieta não alterou (P>0,05) o consumo de água e de ração e o teor de nitrogênio das excretas, entretanto, reduziu (P<0,05) o pH e a umidade das excretas. Conclui-se que a inclusão de 0,5% de zeólitas naturais na dieta de frangos de corte reduz o pH e a umidade das excretas e pode ser utilizada como aditivo alimentar sem prejudicar o consumo de água e de ração.(AU)

The aim of this study was to evaluate the addition of 0.5% natural zeolites (clinoptilolite) to the diet of broilers and their effect on water and feed intake and excreta characteristics. The test was carried out in metabolic cages, with commercial line broilers, in the period from 14 to 23 days old. The experiment was conducted with two groups of broilers: one group received diet without zeolite inclusion (control) and the other received a diet with 0.5% inclusion of zeolite (clinoptilolite) in balanced diets, in a completely randomized design, with 10 replicates of 10 broilers. Water and feed intake, pH, total nitrogen, and moisture of excreta were evaluated. The inclusion of 0.5% of natural zeolites in the diet did not change (P>0.05) the water and feed intake and total nitrogen of excreta, nevertheless, it decreased (P<0.05) the pH and moisture of excreta. We conclude that the addition of 0.5% natural zeolites to the diet of broilers decreases pH and moisture of excreta and can be used as feed additive without compromising water and feed intake.(AU)

Lack of serotonin 5-HT2B receptor alters proliferation and network volume of interstitial cells of Cajal in vivo.

BACKGROUND: Normal gastrointestinal motility requires intact networks of interstitial cells of Cajal (ICC). Interstitial cells of Cajal numbers are maintained by a balance between cell loss factors and survival/trophic/growth factors. Activation of 5-HT(2B) receptors expressed on ICC increases ICC proliferation in vitro. It is not known whether 5-HT(2B) receptors on ICC are activated in vivo. The aims of this study were to investigate if adult ICC proliferate, whether the proliferation of ICC in vivo is affected by knocking out the 5-HT(2B) receptor, and if alterations in proliferation affect ICC networks. METHODS: Proliferating ICC were identified by immunoreactivity for Ki67 in both the myenteric and deep muscular plexus regions of the jejunum in mice with a targeted insertion of a neomycin resistance cassette into the second coding exon of the htr2b receptor gene. KEY RESULTS: Adult ICC do proliferate. The number of proliferating ICC was lower in the myenteric plexus region of Htr2b(-/-) compared to Htr2b(+/+) mice. The volume of Kit-positive ICC was 30% lower in the myenteric plexus region and 40% lower in the deep muscular plexus region in Htr2b(-/-) mice where the number of ICC was also reduced. CONCLUSIONS & INFERENCES: Interstitial cells of Cajal proliferate in adult mice and activation of 5-HT(2B) receptors results in increased proliferation of ICC in vivo. Furthermore, lack of 5-HT(2B) receptor signaling reduces the density of ICC networks in mature mice. These data suggest that 5-HT(2B) receptor signaling is required for maintenance of ICC networks, adding 5-HT to the growing number of factors shown to regulate ICC networks.

Effect of age on the enteric nervous system of the human colon.

The effect of age on the anatomy and function of the human colon is incompletely understood. The prevalence of disorders in adults such as constipation increase with age but it is unclear if this is due to confounding factors or age-related structural defects. The aim of this study was to determine number and subtypes of enteric neurons and neuronal volumes in the human colon of different ages. Normal colon (descending and sigmoid) from 16 patients (nine male) was studied; ages 33-99. Antibodies to HuC/D, choline acetyltransferase (ChAT), neuronal nitric oxide synthase (nNOS), and protein gene product 9.5 were used. Effect of age was determined by testing for linear trends using regression analysis. In the myenteric plexus, number of Hu-positive neurons declined with age (slope = -1.3 neurons/mm/10 years, P = 0.03). The number of ChAT-positive neurons also declined with age (slope = -1.1 neurons/mm/10 years of age, P = 0.02). The number of nNOS-positive neurons did not decline with age. As a result, the ratio of nNOS to Hu increased (slope = 0.03 per 10 years of age, P = 0.01). In the submucosal plexus, the number of neurons did not decline with age (slope = -0.3 neurons/mm/10 years, P = 0.09). Volume of nerve fibres in the circular muscle and volume of neuronal structures in the myenteric plexus did not change with age. In conclusion, the number of neurons in the human colon declines with age with sparing of nNOS-positive neurons. This change was not accompanied by changes in total volume of neuronal structures suggesting compensatory changes in the remaining neurons.

Do Ringtailed lemurs (Lemur catta) reconcile in the hour post-conflict?: A pilot study.

A previous study byKAPPELER (1993) showed that no reconciliation occurred in the ringtailed lemur (Lemur catta). Like the majority of studies dealing with reconciliation, the post-conflict period lasted 10 minutes. An identical study using a 70-min post-conflict period was conducted on one of the same semi-free ranging group studied six years earlier byKAPPELER. Results showed that individuals who never interacted within 10 min did engage in friendly interactions in the longer lasting postconflict period.

Marking behaviour in two lemur species (L. fulvus and L. macaco): relation to social status, reproduction, aggression and environmental change.

The influence of social status, reproductive and agonistic contexts and environmental change on scent-marking and allomarking rates were studied in captive groups of Lemur fulvus and Lemur macaco. No evidence of female social dominance over males appeared in either species. In L. fulvus, intrasexual relationships were characterized by the dominance of one adult male over another and by the existence of female affiliative association ('central' vs. 'peripheral' females). In L. macaco, no intrasexual dominance relationships were apparent except for a brief ostracism of one female. In both species, (1) intrasexual differences in social status were related to differences in marking rates, (2) no direct relation appeared between marking rates and aggression or reproduction, (3) allomarking was not differentially directed towards specific individuals and (4) physical environmental factors clearly influenced scent-marking and allomarking rates. These results are discussed in relation to the possible functions of marking behaviour in intragroup relations.