Assuntos
Endotélio Vascular/virologia , Herpesvirus Humano 1/fisiologia , Herpesvirus Humano 2/fisiologia , Transplante Heterólogo , Replicação Viral , Animais , Linhagem Celular/virologia , Chlorocebus aethiops , Endotélio Vascular/citologia , Herpesvirus Humano 1/patogenicidade , Herpesvirus Humano 2/patogenicidade , Humanos , Suínos , Células VeroRESUMO
The ELISA technique was applied to assess Toxoplasma gondii antibodies in pigs. Among 925 swine examined 36.4 per cent of the animals were seropositive. Serum titres ranged from 100 to 3,200.
Assuntos
Anticorpos Antiprotozoários/sangue , Doenças dos Suínos/diagnóstico , Toxoplasma/imunologia , Toxoplasmose Animal/diagnóstico , Animais , Ensaio de Imunoadsorção Enzimática , Valor Preditivo dos Testes , SuínosRESUMO
The immunocompetency of lymphoid cells from Marek's disease lymphomas was investigated by testing their ability to mount a graft-versus-host reaction in chick embryos, and to produce haemolysins after immunization of the donors with sheep erythrocytes. The results suggested that the lymphomas are complex in origin, containing lymphoid cells derived from both the thymus- and bursa-dependent lymphoid systems.
RESUMO
Application of a direct immunoperoxidase technique for the detection of Derzsy's disease virus antigen in cell culture and goslings is described. Anti-virus globulins were labelled with horseradish peroxidase by use of sodium periodate as a coupling agent. Virus antigen was detected in the nuclei of goose embryo fibroblasts from 48 to 72 hours post-inoculation and in the nuclei of hepatic cells between 5 to 8 days after infection. Microstructural detail was much better in cell culture than in the liver.
RESUMO
OBJECTIVE: The efficacy of a home practice model for gait training was evaluated in 18 hemiplegic subjects 2.3 years (range, 1 to 5) after stroke. DESIGN: Uncontrolled case series. SETTING: Referral center. SUBJECTS: Patients at least 1 year poststroke referred to an outpatient rehabilitation program. INTERVENTION: Patients were taught home programs in two or more 2- to 5-day blocks averaging 35 physical therapy (PT) contact hours (range, 9.5 to 62.5); training extended over a mean of 22 months (range, 10 to 65). Training emphasized weight bearing, balance, segmental control, stretching, and bracing. MAIN OUTCOME MEASURES: Gait changes were measured using the newly developed Wisconsin Gait Scale (WGS). The patient-rated Falls Efficacy Scale (FES) was administered before and after training to 8 subjects, and the Health Status Questionnaire (HSQ) was retrospectively administered to all subjects to appraise subjective pretraining to posttraining changes and current psychological status. RESULTS: The average WGS score significantly improved (p < .05). Patients perceived that gait training increased the quality of their functional activities (p < .05). In a subset of patients, the FES showed that fear of falling was decreased (p < .05). Perception of well-being was comparable to a normative nonstroke reference population except for physical functioning. Compared to the only other published series (using traditional outpatient programming), the current model was of comparable cost. CONCLUSION: Despite the literature indicating a plateau in mobility function by 6 months after stroke, postacute training of gait in hemiplegic subjects using a home-based training model results in improved gait and the perception of improved function. Additionally, we provide validation for the newly developed Wisconsin Gait Scale, an instrument of gait measurement that may assist in comparing outcomes.