RESUMO
CONTEXT: Circulating tumor DNA (ctDNA) is a promising biomarker in cancer. MATERIALS AND METHODS: We generated xenograft models of cancer and detected ctDNA in plasma by qRCR targeting human AluJ sequences. RESULTS: Our assay reached single cell sensitivity in vitro and a correlation between ctDNA amount and tumor size was observed in vivo. Treatment with a mitogen activated protein kinase kinase (MEK)-inhibitor (BAY 869766) reduced ctDNA levels. Using this assay, we also confirmed that high levels of cell-free DNA are found in cancer patients compared to healthy individuals. DISCUSSION AND CONCLUSION: We show that ctDNA may be useful biomarker for monitoring tumor growth and treatment response.
Assuntos
Biomarcadores Tumorais/sangue , Neoplasias da Mama/sangue , Neoplasias Colorretais/sangue , DNA/sangue , Ensaios Antitumorais Modelo de Xenoenxerto/métodos , Animais , Antineoplásicos/administração & dosagem , Neoplasias da Mama/tratamento farmacológico , Neoplasias da Mama/patologia , Estudos de Casos e Controles , Linhagem Celular Tumoral , Neoplasias Colorretais/tratamento farmacológico , Neoplasias Colorretais/patologia , Difenilamina/administração & dosagem , Difenilamina/análogos & derivados , Feminino , Humanos , Cinética , Masculino , Camundongos , Camundongos Nus , Reação em Cadeia da Polimerase em Tempo Real/normas , Padrões de Referência , Sulfonamidas/administração & dosagem , Carga TumoralRESUMO
PURPOSE: To compare the influence of aerobic and resistance exercise on intraocular pressure (IOP). METHODS: Twenty-one healthy subjects participated. Aerobic exercise was performed using a cycle ergometer, and resistance exercise was performed with a leg curl and a butterfly machine. Intraocular pressure was measured at baseline, during exercise and 10 min after. During resistance exercise, a Valsalva manoeuvre was prevented. RESULTS: Before aerobic exercise, the mean IOP was 18.8±2.7 mmHg. It was 16.5±2.8 after 10, 17.1±2.6 after 20 and 16.7±3.3 mmHg after 30 min of exercise. After 10 min, the IOP returned to baseline (18.8 ±2.7 mmHg). The mean IOP before resistance exercise with the leg curl machine was 17.0 (15.6-18.4; 65%Wmax ) and 16.8 (15.3-18.3) mmHg; 75%Wmax ) and did not change significantly during the experiment. The mean IOP before resistance exercise with the butterfly machine (65%Wmax ) was 16.4 (15.2-17.6) and increased to 17.2 (16.0-18.4) mmHg (p<0.05). After 10 min of recreation, it recovered to 16.3 (15.0-17.5) mmHg. At 75%Wmax , the mean baseline IOP was 16.3 (15.2-17.4) mmHg, and there were no significant changes. CONCLUSIONS: Aerobic exercise leads to a significant decrease of IOP. There was no influence of resistance exercise on IOP as long as a Valsalva manoeuvre was prevented.