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1.
Khirurgiia (Mosk) ; (7): 80-87, 2023.
Artigo em Russo | MEDLINE | ID: mdl-37379409

RESUMO

OBJECTIVE: To reconstruct the events of early period of Soviet school of combustiology (1920-1930s) via analysis and systematization of clinical and experimental studies devoted to burns and performed in the Leningrad medical institutes in 1920-1930s. MATERIAL AND METHODS: We analyzed various reports by employees of the Leningrad medical institutes devoted to practice and theory of burn treatment within the above-mentioned historical period. RESULTS: Analysis of Soviet and foreign reports for 1920-1930s made it possible to systematize data on the treatment of burns in the Leningrad medical institutes for the period from the mid-1920s to the beginning of the Great Patriotic War. We presented experimental data on local and general processes following burn injuries. CONCLUSION: We discovered and introduced into scientific circulation some reports of Leningrad scientists on clinical and theoretical aspects of burn injuries which fell out of the field of view of modern researchers for various reasons. These data emphasize diverse work carried out by the staff of surgical and theoretical departments regarding the treatment of burn injuries.


Assuntos
Queimaduras , Hospitais , Humanos , Queimaduras/diagnóstico , Queimaduras/cirurgia , Transplante de Pele
2.
Dokl Biochem Biophys ; 502(1): 21-24, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-35275301

RESUMO

The segment-specific regulatory domains of the Bithorax complex (BX-C), which consists of three homeotic genes Ubx, abd-A and Abd-B, are separated by boundaries that function as insulators. Most of the boundaries contain binding sites for the architectural protein CTCF, which is conserved for higher eukaryotes. As was shown previously, the CTCF sites determine the insulator activity of the boundaries of the Abd-B regulatory region. In this study, it was shown that fragments of the Fab-3 and Fab-4 boundaries of the abd-A regulatory region, containing CTCF binding sites, are not effective insulators.


Assuntos
Fator de Ligação a CCCTC , Proteínas de Drosophila , Drosophila melanogaster , Animais , Sítios de Ligação , Fator de Ligação a CCCTC/genética , Fator de Ligação a CCCTC/metabolismo , Proteínas de Drosophila/genética , Proteínas de Drosophila/metabolismo , Drosophila melanogaster/genética , Drosophila melanogaster/metabolismo , Genes Homeobox , Proteínas de Homeodomínio/genética , Proteínas de Homeodomínio/metabolismo , Elementos Isolantes , Fatores de Transcrição/metabolismo
3.
Dokl Biol Sci ; 501(1): 197-200, 2021 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-34962606

RESUMO

Proteins with clusters of C2H2 zinc finger domains (C2H2-proteins) constitute the most abundant class of transcription factors in higher eukaryotes. N-terminal ZAD (zinc finger-associated domain) dimerization domain has been identified in a large group of C2H2-proteins mostly in insects. The piragua gene encodes one of these proteins, Fu2. We have generated CRISPR/Cas9-mediated deletion of the piragua gene that has no phenotype. We have used φC31-mediated attP/attB recombination to generate a transgenic line expressing Fu2 protein fused with HA epitope. This line will be useful for analysis of DNA binding profile and functions of Fu2 protein.


Assuntos
Proteínas de Transporte/genética , Proteínas de Drosophila/genética , Drosophila , Animais , Drosophila/genética , Drosophila/crescimento & desenvolvimento , Fatores de Transcrição/genética , Dedos de Zinco/genética
4.
Artigo em Russo | MEDLINE | ID: mdl-34882334

RESUMO

The article presents and analyzes functional results of intravitreal application of two angiogenesis inhibitors of Ranibizumab and Aflibercept in treatment of diabetic macular edema. The positive results were obtained after triple administration of preparation with monthly intervals.


Assuntos
Diabetes Mellitus , Retinopatia Diabética , Edema Macular , Inibidores da Angiogênese/farmacologia , Inibidores da Angiogênese/uso terapêutico , Retinopatia Diabética/tratamento farmacológico , Humanos , Injeções Intravítreas , Edema Macular/tratamento farmacológico , Edema Macular/etiologia , Ranibizumab/uso terapêutico , Estudos Retrospectivos , Resultado do Tratamento
5.
Dokl Biochem Biophys ; 491(1): 98-100, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32483761

RESUMO

The paper briefly describes the evolution of the key enzyme of photosynthesis, RuBisCO. Before the emergence of the reaction of carbon dioxide assimilation via photosynthesis, this protein was involved in the methionine metabolism chain. Possibly, for this reason, the carboxylation reaction catalyzed by enzyme proceeds very slowly. In addition to carboxylation, RuBisCO can simultaneously oxidize ribulose bisphosphate, a substrate to which the fixed CO2 is attached. This, in turn, also reduces the effectiveness of photosynthesis. In this regard, the literature discusses various options for increasing plant productivity by creating new forms of RuBisCO or fundamentally different pathways of carbon dioxide assimilation. In this work, we propose a modification of the carboxylation reaction that makes it possible to avoid photorespiration and thus increase the efficiency of photosynthesis.


Assuntos
Bacillus subtilis/metabolismo , Dióxido de Carbono/química , Fotossíntese , Ribulose-Bifosfato Carboxilase/química , Ribulosefosfatos/química , Carbono/química , Catálise , Cinética , Oxigênio/química , Fotoquímica
6.
Angiol Sosud Khir ; 25(1): 115-119, 2019.
Artigo em Russo | MEDLINE | ID: mdl-30994617

RESUMO

Contemporary approaches to treatment of patients with dissection of the descending aorta (DA), as well as the data of international registries and guidelines are contradictory. The endovascular method of treatment of patients with descending aortic dissection has been recognized to be revolutionary. However, there seems to be no commonly accepted concept concerning appropriate use of currently available techniques of treating patients with DA dissection. This study was aimed at analysing the experience gained in using various methods of treatment of patients with descending aortic dissection (DAD). We retrospectively examined the results of surgical treatment of 78 patients with DAD. Of these, 45 patients were found to have Stanford type B dissection and 33 patients had type A dissection, who underwent treatment during ten years from January 2008. The decision concerning the type of care for patients with DAD (conservative, surgical, endovascular or combined methods of treatment) directly depends on the patient's state stability, technical feasibility of the intended procedure, and the presence of concomitant pathology. The findings obtained in our study demonstrated better immediate outcomes of the endovascular approach compared with the hybrid and surgical ones. In order to achieve complete haemodynamic correction of the pathology, to minimize the risk of aortic remodelling and the development of aorta-related complications, the treatment should not be limited to endoprosthetic repair of the thoracic portion of the DA with a stent graft, but should rather be supplemented with stenting of more distal segments, including with uncoated stents at the level of the visceral branches. Only such staged approach may provide better conditions for obliteration of the aortic false lumen and formation of a 'neo-frame' of the aortic wall.


Assuntos
Aneurisma da Aorta Torácica , Dissecção Aórtica , Implante de Prótese Vascular , Procedimentos Endovasculares , Dissecção Aórtica/terapia , Aneurisma da Aorta Torácica/terapia , Humanos , Complicações Pós-Operatórias , Estudos Retrospectivos , Stents , Resultado do Tratamento
7.
J Intern Med ; 284(5): 546-559, 2018 11.
Artigo em Inglês | MEDLINE | ID: mdl-29964306

RESUMO

BACKGROUND AND OBJECTIVES: Bile acids (BAs) traversing the enterohepatic circulation (EHC) influence important metabolic pathways. By determining individual serum BAs in relation to markers of metabolic activity, we explored how diurnal variations in their EHC relate to hepatic metabolism in normal humans. METHODS: Serum BAs, fibroblast growth factor 19 (FGF19), lipoproteins, glucose/insulin and markers of cholesterol and BA syntheses were monitored for 32 h in 8 healthy males. Studies were conducted at basal state and during initiation of cholestyramine treatment, with and without atorvastatin pretreatment. Time series cross-correlation analysis, Bayesian structural model and Granger causality test were applied. RESULTS: Bile acids synthesis dominated daytime, and cholesterol production at night. Conjugated BAs peaked after food intake, with subsequent FGF19 elevations. BA synthesis was reduced following conjugated BA and FGF19 peaks. Cholestyramine reduced conjugated BAs and FGF19, and increased BA and cholesterol production; the latter effects attenuated by atorvastatin. The relative importance of FGF19 vs. conjugated BAs in this feedback inhibition could not be discriminated. Unconjugated BAs displayed one major peak late at night/early morning that was unrelated to FGF19 and BA synthesis, and abolished by cholestyramine. The normal suppression of serum triglycerides, glucose and insulin observed at night was attenuated by cholestyramine. CONCLUSIONS: Conjugated and unconjugated BAs have asynchronous rhythms of EHC in humans. Postprandial transintestinal flux of conjugated BAs increases circulating FGF19 levels and suppresses BA synthesis. Unconjugated BAs peak late at night, indicating a non-postprandial diurnal change in human gut microflora, the physiological implications of which warrants further study.


Assuntos
Ácidos e Sais Biliares/metabolismo , Ritmo Circadiano , Redes e Vias Metabólicas , Adulto , Anticolesterolemiantes/farmacologia , Atorvastatina/farmacologia , Ácidos e Sais Biliares/sangue , Ácidos e Sais Biliares/fisiologia , Biomarcadores/sangue , Glicemia/análise , Resina de Colestiramina/farmacologia , Ritmo Circadiano/fisiologia , Fatores de Crescimento de Fibroblastos/sangue , Humanos , Insulina/sangue , Lipoproteínas/sangue , Fígado/efeitos dos fármacos , Fígado/metabolismo , Masculino , Redes e Vias Metabólicas/efeitos dos fármacos , Redes e Vias Metabólicas/fisiologia , Pessoa de Meia-Idade , Adulto Jovem
8.
Mol Biol (Mosk) ; 52(2): 326-332, 2018.
Artigo em Russo | MEDLINE | ID: mdl-29695702

RESUMO

Nowadays, allergic disorders have become one of the most important social problems in the world. This can be related to the advent of new allergenic agents in the environment, as well as an increasing density of human contact with known allergens, including various proteins. Thus, the development of computer programs designed for the prediction of allergenic properties of proteins becomes one of the urgent tasks of mo dern bioinformatics. Previously we developed a web accessible Allpred Program (http://www-bionet.sscc.ru/ psd/cgi-bin/programs/Allpred/allpred.cgi) that allows users to assess the allergenicity of proteins by taking into account the characteristics of their spatial structure. In this paper, using AllPred, we predicted the allergenicity of proteins from 462 archaea and bacteria species for which a complete genome was available. The segregation of considered proteins on archaea and bacteria has shown that allergens are predicted more often among archaea than among bacteria. The division of these proteins into groups according to their intracellular localization has revealed that the majority of allergenic proteins were among the secreted proteins. The application of methods for predicting the level of gene expression of microorganisms based on DNA sequence analysis showed a statistically significant relationship between the expression level of the proteins and their allergenicity. This analysis has revealed that potentially allergenic proteins were more common among highly expressed proteins. Sorting microorganisms into the pathogenic and nonpathogenic groups has shown that pathogens can potentially be more allergenic because of a statistically significant greater number of allergens predicted among their proteins.


Assuntos
Archaea/imunologia , Proteínas Arqueais/imunologia , Bactérias/imunologia , Proteínas de Bactérias/imunologia , Hipersensibilidade/imunologia , Modelos Imunológicos , Software , Humanos , Hipersensibilidade/patologia , Valor Preditivo dos Testes
9.
Dokl Biochem Biophys ; 483(1): 293-296, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30607723

RESUMO

Using the patch-clamp method in the whole-cell configuration, we showed that the new derivatives of 2-aminothiophene-3-carboxylic acid, which were synthesized by us earlier, can both block (compound 1) and potentiate (compound 2) calcium-activated chloride currents in single rat cerebellar Purkinje cells.


Assuntos
Canais de Cloreto/metabolismo , Potenciais da Membrana/efeitos dos fármacos , Células de Purkinje/metabolismo , Toluidinas , Animais , Células Cultivadas , Ratos , Toluidinas/síntese química , Toluidinas/química , Toluidinas/farmacologia
10.
Vestn Oftalmol ; 134(3): 35-41, 2018.
Artigo em Russo | MEDLINE | ID: mdl-29953080

RESUMO

Prediction of the clinical course of primary open-angle glaucoma (POAG) is one of the main directions in solving the problem of vision loss prevention and stabilization of the pathological process. Simple statistical methods of correlation analysis show the extent of each risk factor's impact, but do not indicate the total impact of these factors in personalized combinations. The relationships between the risk factors is subject to correlation and regression analysis. The regression equation represents the dependence of the mathematical expectation of the resulting sign on the combination of factor signs. PURPOSE: To develop a technique for predicting the probability of development and progression of primary open-angle glaucoma based on a personalized combination of risk factors by linear multivariate regression analysis. MATERIAL AND METHODS: The study included 66 patients (23 female and 43 male; 132 eyes) with newly diagnosed primary open-angle glaucoma. The control group consisted of 14 patients (8 male and 6 female). Standard ophthalmic examination was supplemented with biochemical study of lacrimal fluid. Concentration of matrix metalloproteinase MMP-2 and MMP-9 in tear fluid in both eyes was determined using 'sandwich' enzyme-linked immunosorbent assay (ELISA) method. RESULTS: The study resulted in the development of regression equations and step-by-step multivariate logistic models that can help calculate the risk of development and progression of POAG. Those models are based on expert evaluation of clinical and instrumental indicators of hydrodynamic disturbances (coefficient of outflow ease - C, volume of intraocular fluid secretion - F, fluctuation of intraocular pressure), as well as personalized morphometric parameters of the retina (central retinal thickness in the macular area) and concentration of MMP-2 and MMP-9 in the tear film. CONCLUSION: The newly developed regression equations are highly informative and can be a reliable tool for studying of the influence vector and assessment of pathogenic potential of the independent risk factors in specific personalized combinations.


Assuntos
Glaucoma de Ângulo Aberto , Humor Aquoso , Progressão da Doença , Feminino , Glaucoma de Ângulo Aberto/diagnóstico , Humanos , Pressão Intraocular , Modelos Lineares , Masculino , Tonometria Ocular , Transtornos da Visão , Campos Visuais
11.
Khirurgiia (Mosk) ; (3. Vyp. 2): 27-32, 2018.
Artigo em Russo | MEDLINE | ID: mdl-29652319

RESUMO

AIM: To assess the possibilities of modern diagnostic techniques to diagnose precancerous pathology and early central lung cancer. MATERIAL AND METHODS: Analysis of Russian and foreign publications for precancerous pathology and early lung cancer, results and comparison of various diagnostic techniques. RESULTS: Central lung cancer has a stepwise development with transformation of normal bronchial epithelium to hyperplastic followed by focal metaplasia, dysplasia, cancer in situ (CIS) and microinvasive cancer. Fluorography, chest X-ray, computed tomography, magnetic resonance imaging and sputum cytology examination are used to diagnose lung cancer. However, endoscopy plays a leading role in diagnosis of early central lung cancer which rapidly and effectively detects changes of tracheobronchial tree mucosa at the initial stages of carcinogenesis thanks to the use of modern techniques (spectral, autofluorescent and ZOOM - endoscopy).


Assuntos
Neoplasias Pulmonares/diagnóstico , Lesões Pré-Cancerosas/diagnóstico , Humanos , Neoplasias Pulmonares/patologia , Lesões Pré-Cancerosas/patologia
12.
J Virol ; 90(1): 575-85, 2016 01 01.
Artigo em Inglês | MEDLINE | ID: mdl-26468548

RESUMO

UNLABELLED: Influenza virus is taken up from a pH-neutral extracellular milieu into an endosome, whose contents then acidify, causing changes in the viral matrix protein (M1) that coats the inner monolayer of the viral lipid envelope. At a pH of ~6, M1 interacts with the viral ribonucleoprotein (RNP) in a putative priming stage; at this stage, the interactions of the M1 scaffold coating the lipid envelope are intact. The M1 coat disintegrates as acidification continues to a pH of ~5 to clear a physical path for the viral genome to transit from the viral interior to the cytoplasm. Here we investigated the physicochemical mechanism of M1's pH-dependent disintegration. In neutral media, the adsorption of M1 protein on the lipid bilayer was electrostatic in nature and reversible. The energy of the interaction of M1 molecules with each other in M1 dimers was about 10 times as weak as that of the interaction of M1 molecules with the lipid bilayer. Acidification drives conformational changes in M1 molecules due to changes in the M1 charge, leading to alterations in their electrostatic interactions. Dropping the pH from 7.1 to 6.0 did not disturb the M1 layer; dropping it lower partially desorbed M1 because of increased repulsion between M1 monomers still stuck to the membrane. Lipid vesicles coated with M1 demonstrated pH-dependent rupture of the vesicle membrane, presumably because of the tension generated by this repulsive force. Thus, the disruption of the vesicles coincident with M1 protein scaffold disintegration at pH 5 likely stretches the lipid membrane to the point of rupture, promoting fusion pore widening for RNP release. IMPORTANCE: Influenza remains a top killer of human beings throughout the world, in part because of the influenza virus's rapid binding to cells and its uptake into compartments hidden from the immune system. To attack the influenza virus during this time of hiding, we need to understand the physical forces that allow the internalized virus to infect the cell. In particular, we need to know how the protective coat of protein inside the viral surface reacts to the changes in acid that come soon after internalization. We found that acid makes the molecules of the protein coat push each other while they are still stuck to the virus, so that they would like to rip the membrane apart. This ripping force is known to promote membrane fusion, the process by which infection actually occurs.


Assuntos
Vírus da Influenza A/efeitos dos fármacos , Vírus da Influenza A/fisiologia , Proteínas da Matriz Viral/metabolismo , Internalização do Vírus/efeitos dos fármacos , Adsorção , Fenômenos Químicos , Humanos , Concentração de Íons de Hidrogênio , Bicamadas Lipídicas/metabolismo , Ligação Proteica , Conformação Proteica/efeitos dos fármacos , Multimerização Proteica , Eletricidade Estática
13.
Genetika ; 52(1): 66-73, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27183794

RESUMO

Soybean is recognized as one of the plants which are very difficult to be transformed. Considering the low transformation efficiency of soybean, we aimed to determine the effect of 6-benzylaminopurine (6-BA), shoot induction time, and infection time of Agrobacterium on the clonal propagation of Glycine max. Results showed that 1.6 mg/L 6-BA could be optimal to promote the induction of adventitious shoots. An induction time of 15 d was considered optimal for the actual experiment involving soybean shoot induction. Agrobacterium was cultured until an OD600 = 0.8 was reached for an infection time of 30 min; this infection time may be optimal to promote soybean transformation. Whole genome DNA methylation was analyzed by high-performance liquid chromatography (HPLC)-assisted quantification, and DNA methylation result is consistent with the phenotypic data of shoot development. In addition, two methylation-related genes (Decrease in DNA methylation 1 and DNA methyltransferases chromomethylase 2) were analyzed to determine expression differences by qRT-PCR in the shoots that were developed under different experimental conditions. In general, the expression values of these genes were normally downregulated under the recommended experimental conditions of soybean regeneration. This study showed the overall methylation changes in the in vitro culture of soybean, as affected by several variable parameters, which is useful to promote the transformation efficiency of soybean.


Assuntos
Agrobacterium tumefaciens/genética , Metilação de DNA/genética , Glycine max/genética , Transformação Genética/efeitos dos fármacos , Compostos de Benzil , Metilação de DNA/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas , Genoma de Planta/efeitos dos fármacos , Cinetina/farmacologia , Metiltransferases/biossíntese , Plantas Geneticamente Modificadas/efeitos dos fármacos , Purinas , Glycine max/crescimento & desenvolvimento , Glycine max/microbiologia
14.
Bull Exp Biol Med ; 160(4): 455-8, 2016 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-26902352

RESUMO

Psychotropic properties of CA-7043× and CA-7050×, new fluorinated derivatives of tetrahydrocarbasoles, were examined on outbred CD1 mice and transgenic 5×FAD mice with Alzheimer disease. Both agents exerted cognitive-stimulating and anxiolytic effects in a dose of 5 mg/kg. In the new cage test, they retarded extinction of orientation and exploratory behavior. CA-7043× produced an anxiolytic effect on CD1 mice assessed in the open-field test and exerted cognitive-stimulating action in the new location test. In the same tests, CA-7050× demonstrated the cognitive-stimulating and anxiolytic effects on transgenic 5×FAD mice.


Assuntos
Ansiolíticos/farmacologia , Ansiedade/tratamento farmacológico , Comportamento Animal/efeitos dos fármacos , Carbazóis/farmacologia , Comportamento Exploratório/efeitos dos fármacos , Hidrocarbonetos Fluorados/farmacologia , Aprendizagem em Labirinto/efeitos dos fármacos , Psicotrópicos/farmacologia , Doença de Alzheimer/tratamento farmacológico , Animais , Modelos Animais de Doenças , Masculino , Camundongos , Camundongos Transgênicos
15.
Vestn Oftalmol ; 132(1): 10-14, 2016.
Artigo em Russo | MEDLINE | ID: mdl-27030428

RESUMO

AIM: to study the CFH T402H polymorphism in glaucoma patients and controls. MATERIAL AND METHODS: Genetic analysis was performed in 68 patients with primary open-angle glaucoma (POAG) of various severity. The control group consisted of 30 participants. Venous whole blood samples were obtained. From them, leukocytes were isolated and human genomic DNA extracted for further PCR. RESULTS: None of the patients from either group was homozygous for the 402H allele. With ganglion cells death (i.e. at later stages of the disease), the percentage of homozygotes that carry no CFH polymorphism increases up to 65%, while that of heterozygotes decreases down to 35%. CONCLUSION: As shown, most of early and advanced POAG patients are heterozygotes. At these stages of the disease own ganglion cells are very likely to be damaged. Further progression, however, is associated with a gradual decrease in heterozygotes (down to 35%) due to a substantial loss of neuroepithelial cells and suppression of the autoimmune response which has lost its target.

16.
Voen Med Zh ; 337(7): 38-43, 2016 07.
Artigo em Russo | MEDLINE | ID: mdl-30590891

RESUMO

Bum injuries in pregnant women: prevalence, structure, outcomes. Determining the optimal treatment strategy and the subsequent treatment of pregnant women with burns require the collaborative work of doctors of various specialties with relevant experience and high qualification. There are no so many researches on this issue, and most of them is written in the second half of the last century. In this regard, the authors compiled and analysed available on the considered issues of data presented in the foreign literature from 1958 to the present.


Assuntos
Queimaduras , Complicações na Gravidez , Adolescente , Adulto , Queimaduras/epidemiologia , Queimaduras/fisiopatologia , Queimaduras/terapia , Feminino , Humanos , Gravidez , Complicações na Gravidez/epidemiologia , Complicações na Gravidez/fisiopatologia , Complicações na Gravidez/terapia , Estudos Retrospectivos
17.
Eksp Klin Gastroenterol ; (10): 80-85, 2016.
Artigo em Inglês, Russo | MEDLINE | ID: mdl-29889379

RESUMO

OBJECTIVE: the aim of study was testing and introduction in clinical practice combination of EUS with sonoelastography, fine-needle biopsy and needle-based confocal laser endomicroscopy in advanced diagnostic of pancreatic disease. MATERIALS AND METHODS: in the period from February 2014 to December 2015, we accumulated experience of EUS-FNA in 72 patients mostly with tumor pathology of the pancreas. In 16 cases we used 19G, in 49-22G, and in 7 patients - 25G needles. In 5 patients we used COOK Echotip Procore needle. In 8 cases we performed confocal laser endomicroscopy through the 19G needle (nCLE) in 3 patients with cystic and in 5 - with solid tumors. In 20 patients, there we used.HITA-CHI-PENTAX sonoelastography was performed. RESULTS: There were no complications in EUS-FNA. In all cases we received pathology verification of disease. nCLE results always confirmed by morphological examination of material. In elastography examination normal pancreatic tissue had a equable green-yellow color, in chronic pancreatitis on the same background there were areas of blue seal parenchyma, in the cases pancreatic adenocarcinoma it was marked predominance of blue color scale, in neuroendocrine tumors - diffuse distribution of green and blue areas. Using nCLE in patients with adenocarcinoma revealed the destruction of certain glands, polymorphic nuclei of epithelial cells, with their enlargement, deformation, loss of polarity. Appliance of Procore needle has its technical features, but allowed us to obtain more material with less bloody - due to fewer number of passes. CONCLUSION: Thus, our study confirms the effectiveness of EUS-FNA with sonoelastography and nCLE. It defines objectives for improvement and expansion of the range for their clinical use.


Assuntos
Endoscopia Gastrointestinal/métodos , Neoplasias Pancreáticas/diagnóstico , Adulto , Idoso , Idoso de 80 Anos ou mais , Biópsia por Agulha Fina , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Pancreáticas/patologia
18.
Gig Sanit ; 95(10): 293-9, 2016.
Artigo em Russo | MEDLINE | ID: mdl-29431333

RESUMO

The composition and the structure of microbial communities in areas of Arctic settlements were studied. The main attention has been given to microscopic fungi. As result of observation of 5 Arctic regions 117 species of microscopic fungi are revealed in soils and anthropogenic substrates. The identification was carried out with the use ofmycological and molecular genetic methods. Most ofspecies belong to the Ascomycotina. Genus Penicillium is characterized by the most species diversity (24 species). Most offungi are destructors of various materials and potential human pathogens. Dominant species are revealed. The distribution of microorganisms in the living and working areas of polar stations, as well as the adjacent areas are described. Black-colored fungi adapted to unfavorable environment are often the dominated group of microorganisms on soils and anthropogenic substrates. The shaping of soil microbiota was shown to be related to the anthropogenic impact. Considerable similarity of microbial communities composition in the soil and man-made substrates is fixed. As result of mycological analysis of contaminated soils 76 species of microscopic fungi were observed, but 41 species of them (53.9%) were identified in the areas of Arctic polar stations on the man-made materials. These species include the representatives of the genera Alternaria, Aspergillus, Aureobasidium, Chaetomium, Cladosporium, Exophiala, Geomyces, Humicola, Penicillium, Mucor, Phoma, Rhodotorula, Trichoderma and Ulocladium. The obtained data show a significant similarity in species composition of contaminated soils and anthropogenic substrates. Human activity contributes to the distribution of cosmopolitan species, including opportunistic fungi, in the Arctic region. The high numbers of organotrophic bacteria were revealed in soil samples. Some species of microorganisms produce the organic acids in an external environment that promotes the erosion of materials.


Assuntos
Fungos Mitospóricos , Microbiologia do Solo , Solo/normas , Regiões Árticas/epidemiologia , Biota , Monitoramento Ambiental/métodos , Monitoramento Ambiental/estatística & dados numéricos , Humanos , Microbiota , Fungos Mitospóricos/classificação , Fungos Mitospóricos/isolamento & purificação , Fungos Mitospóricos/fisiologia , Filogeografia , Federação Russa/epidemiologia
19.
Klin Med (Mosk) ; 93(3): 69-74, 2015.
Artigo em Russo | MEDLINE | ID: mdl-26168607

RESUMO

The article dials with the history of medicine practiced by the first civilizations. The facts of discovery and analysis of ancient medical treatises by modern researchers are reported. Special emphasis is laid on the achievements of medical practitioners in Ancient Egypt and India that promoted the development of operative treatment including skin grafting.


Assuntos
Transplante de Pele , Terras Antigas , Civilização/história , História Antiga , Humanos , Transplante de Pele/história , Transplante de Pele/métodos
20.
Klin Med (Mosk) ; 93(5): 65-70, 2015.
Artigo em Russo | MEDLINE | ID: mdl-26410963

RESUMO

The history of skin grafting from the beginning of the Common Era to the XIX centuy is analysed with reference to the contribution made by diferent scientists who performed a search (first intuitive, then theoretically substantiated) for the methods to correct soft tissue defects in different parts of the human body).


Assuntos
Transplante de Pele/história , História do Século XV , História do Século XVI , História do Século XVII , História do Século XVIII , História do Século XIX , História do Século XX , História do Século XXI , História Antiga , História Medieval , Humanos
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