Performance Evaluation of Enzyme Breaker for Fracturing Applications under Simulated Reservoir Conditions.

Fracturing fluids are being increasingly used for viscosity development and proppant transport during hydraulic fracturing operations. Furthermore, the breaker is an important additive in fracturing fluid to extensively degrade the polymer mass after fracturing operations, thereby maximizing fracture conductivity and minimizing residual damaging materials. In this study, the efficacy of different enzyme breakers was examined in alkaline and medium-temperature reservoirs. The parameters considered were the effect of the breaker on shear resistance performance and sand-suspending performance of the fracturing fluid, its damage to the reservoir after gel breaking, and its gel-breaking efficiency. The experimental results verified that mannanase II is an enzyme breaker with excellent gel-breaking performance at medium temperatures and alkaline conditions. In addition, mannanase II did not adversely affect the shear resistance performance and sand-suspending performance of the fracturing fluid during hydraulic fracturing. For the same gel-breaking result, the concentration of mannanase II used was only one fifth of other enzyme breakers (e.g., mannanase I, galactosidase, and amylase). Moreover, the amount of residue and the particle size of the residues generated were also significantly lower than those of the ammonium persulfate breaker. Finally, we also examined the viscosity-reducing capability of mannanase II under a wide range of temperatures (104-158 °F) and pH values (7-8.5) to recommend its best-use concentrations under different fracturing conditions. The mannanase has potential for applications in low-permeability oilfield development and to maximize long-term productivity from unconventional oilwells.

Dynamic investigation of nutrient consumption and injection strategy in microbial enhanced oil recovery (MEOR) by means of large-scale experiments.

Microbial enhanced oil recovery (MEOR) depends on the in situ microbial activity to release trapped oil in reservoirs. In practice, undesired consumption is a universal phenomenon but cannot be observed effectively in small-scale physical simulations due to the scale effect. The present paper investigates the dynamics of oil recovery, biomass and nutrient consumption in a series of flooding experiments in a dedicated large-scale sand-pack column. First, control experiments of nutrient transportation with and without microbial consumption were conducted, which characterized the nutrient loss during transportation. Then, a standard microbial flooding experiment was performed recovering additional oil (4.9 % Original Oil in Place, OOIP), during which microbial activity mostly occurred upstream, where oil saturation declined earlier and steeper than downstream in the column. Subsequently, more oil remained downstream due to nutrient shortage. Finally, further research was conducted to enhance the ultimate recovery by optimizing the injection strategy. An extra 3.5 % OOIP was recovered when the nutrients were injected in the middle of the column, and another additional 11.9 % OOIP were recovered by altering the timing of nutrient injection.

Structural and compositional diversity of biosurfactants produced by a novel strain of Sporosarcina luteola ME44 from oil reservoir.

The urealytically active microorganism Sporosarcina luteola induces the precipitation of metals, which has attracted attention in biomineralization, bioremediation, and industrial waste recycling. Herein, we report a novel biosurfactant-producing strain of S. luteola ME44 isolated from Chinese Oilfield. The structure, composition, and surface activity of the biosurfactants produced by S. luteola ME44 were investigated by using a combination of the high-performance liquid chromatography, time-of-flight mass spectrometry, and surface tensiometer. The biosurfactant extracted by strain ME44 was identified as surfactin with five variants and the yield was 1010 ± 60 mg⋅L-1 . This is the first report on the structural composition and surface activity of biosurfactants isolated from the S. luteola. It extended our knowledge about the role of the species S. luteola in the ecosystem of extreme natural environments such as oil reservoir. In addition, S. luteola ME44 showed bioprecipitation properties for metal ions Cd(II), Cu(II), Zn(II), and Ag(I), which indicated the application potential of S. luteola in the field of bioremediation.

A new biosurfactant-producing strain, Fictibacillus nanhaiensis ME46, isolated from an oil field in China.

The genus Fictibacillus contains twelve species significant in the synthesis of cellulose-degrading enzymes and phenylalanine dehydrogenase, isolated mainly from marine sedimentary environments. Here, we report a new biosurfactant-producing strain, Fictibacillus nanhaiensis ME46, isolated from Daqing oil field in China. The biosurfactant extracted from Strain ME46 was determined as surfactin, one of the representative families of lipopeptide biosurfactants. The yield of the surfactin produced by strain ME46 was 0.62 g·L-1 as determined by high-performance liquid chromatography, and the critical micelle concentration (CMC) of the surfactin was estimated to be about 68 mg·L-1 and the surface tension at CMC was 35.1 mN·m-1. This study extended our knowledge about the role of the species Fictibacillus nanhaiensis in the ecosystem of natural environments such as the oil field.

Structural Diversity of the Lipopeptide Biosurfactant Produced by a Newly Isolated Strain, Geobacillus thermodenitrifcans ME63.

The genus Geobacillus is active in degradation of hydrocarbons in thermophilic and facultative environments since it was first reported in 1920. Here, we report a new strain, Geobacillus thermodenitrificans ME63, isolated from an oilfield with the ability of producing the biosurfactant. The composition, chemical structure, and surface activity of the biosurfactant produced by G. thermodenitrificans ME63 were investigated by using a combination of the high-performance liquid chromatography, time-of-flight ion mass spectrometry, and surface tensiometer. The biosurfactant produced by strain ME63 was identified as surfactin with six variants, which is one of the representative family of lipopeptide biosurfactants. The amino acid residue sequence in the peptide of this surfactin is N-Glu → Leu → Leu → Val → Leu → Asp → Leu-C. The critical micelle concentration (CMC) of the surfactin is 55 mg L-1, and the surface tension at CMC is 35.9 mN m-1, which is promising in bioremediation and oil recovery industries. The surface activity and emulsification properties of biosurfactants produced by G. thermodenitrificans ME63 showed excellent resistance to temperature changes, salinity changes, and pH changes.

Using Vinegar Residue-Based Carrier Materials to Improve the Biodegradation of Phenanthrene in Aqueous Solution.

A large amount of vinegar residue (VR) is generated every year in China, causing serious environmental pollutions. Meanwhile, as a kind of persistent organic pollutants, polycyclic aromatic hydrocarbons (PAHs) ubiquitously exist in environments. With a goal of reusing VR and reducing PAHs pollutions, we herein isolated one B. subtilis strain, ZL09-26, which can degrade phenanthrene and produce biosurfactants. Subsequently, raw VR was dried under different temperatures (50 °C, 80 °C, 100 °C and 120 °C) or pyrolyzed under 350 °C and 700 °C, respectively. After being characterized by various approaches, the treated VR were mixed with ZL09-26 as carriers to degrade phenanthrene. We found that VR dried at 50 °C (VR50) was the best in promoting the growth of ZL09-26 and the degradation of phenanthrene. This result may be attributed to the residual nutrients, suitable porosity and small surface charge of VR50. Our results demonstrate the potential of VR in the biodegradation of phenanthrene, which may be meaningful for developing new VR-based approaches to remove PAHs in aqueous environments.

Root exuded low-molecular-weight organic acids affected the phenanthrene degrader differently: A multi-omics study.

As a class of highly toxic and persistent organic pollutants, polycyclic aromatic hydrocarbons (PAHs) are an increasingly urgent environmental problem. Low-molecular-weight organic acids (LMWOAs) are important factors that regulate the degradation of PAHs by plant rhizosphere microorganisms, which affect the absorption of PAHs by plant roots. However, the comprehensive mechanisms by which LMWOAs influence the biodegradation of PAHs at cellular and omics levels are still unknown. Here, we systematically analyzed the roles of citric, glutaric and oxalic acid in the PAH-degradation process, and investigated the mechanisms through which these three LMWOAs enhance phenanthrene (PHE) biodegradation by B. subtilis ZL09-26. The results showed that LMWOAs can improve the solubility and biodegradation of PHE, enhance cell growth and activity, and relieve membrane and oxidative stress. Citric acid enhanced PHE biodegradation mainly by improving the strain's cell proliferation and activity, while glutaric and oxalic acid accelerated PHE biodegradation mainly by improving the expression of enzymes and providing energy for the cells of B. subtilis ZL09-26. This study provides new insights into rhizospheric bioremediation mechanisms, which may enable the development of new biostimulation techniques to improve the bioremediation of PAHs.