SNHG16/miR-205/HDAC5 is involved in the progression of renal fibrosis.

Renal interstitial fibrosis (RIF) represents an irreversible and progressive pathological manifestation of chronic renal disease, which ultimately leads to end-stage renal disease. Long noncoding RNAs (lncRNAs) have been suggested to be involved in the progression of RIF. Small nucleolar RNA host gene 16 (SNHG16), a member of lncRNAs, has been found to be involved in the progression of pulmonary fibrosis. This paper first researched the effect of SNHG16 on renal fibrosis. We established a unilateral ureteral obstruction (UUO)-induced mouse RIF model by ligation of the left ureter to evaluate the biological function of SNHG16 in RIF. As a result, SNHG16 was upregulated in UUO-induced renal fibrotic tissues. Knockdown of SNHG16 inhibited RIF and reduced alpha-smooth muscle actin (α-SMA), fibronectin, and college IV expression. miR-205 was a target of SNHG16, and downregulated in UUO-induced renal fibrotic tissues. Inhibition of miR-205 promoted RIF and increased the expression of α-SMA, college IV, and fibronectin. Overexpression of SNHG16 promoted the UUO-induced RIF, but miR-205 abrogated this effect of SNHG16. Histone deacetylase 5 (HDAC5) showed high expression in UUO-induced renal fibrotic tissues. Knockdown of HDAC5 significantly reduced α-SMA, fibronectin, and college IV expression in renal tissues of UUO-induced mice. Inhibition of miR-205 promoted HDAC5 expression, but knockdown of SNHG16 inhibited HDAC5 expression in renal tissues of UUO-induced mice. In conclusion, SHNG16 is highly expressed in renal fibrotic tissues of UUO-induced mice. Knockdown of SHNG16 may prevent UUO-induced RIF by indirectly upregulating HDAC5 via targeting miR-205. SHNG16 may be novel target for treating renal fibrosis.

Association between hydration status and the risk and all-cause mortality of diabetic kidney disease.

BACKGROUND: This cohort study aimed to explore the relationship between hydration status and the risk of diabetic kidney disease (DKD) as well as all-cause death in DKD patients. METHODS: Weighted univariable and multivariable logistic regression models were used to explore the association between hydration status and DKD risk in diabetic population while weighted univariable and multivariable Cox regression models were used to identify the association between hydration status and all-cause mortality in DKD patients. Kaplan-Meier curve was plotted to present the survival probability of patients with different hydration status. Estimates were presented as odds ratio (OR), and hazard ratio (HR) with 95% confidence interval (CI). RESULTS: The mean follow-up time was 79.74 (±1.89) months. There were 2041 participants with DKD, and 2889 participants without. At the end of the follow-up, 965 participants were alive. The risk of DKD was increased as the increase of osmolarity level (OR = 1.07, 95%CI: 1.05-1.08). The elevated risk of DKD was observed in patients with impending dehydration (OR = 1.49, 95%CI: 1.19-1.85) or current dehydration (OR = 2.69, 95%CI: 2.09-3.46). The association between increased osmolarty level and elevated risk of all-cause mortality in DKD patients was statistically different (HR = 1.02, 95%CI: 1.01-1.03). Current dehydration was correlated with increased all-cause mortality risk in DKD patients (HR = 1.27, 95%CI: 1.01-1.61). Compared to DKD patients with normal hydration, the survival probability of DKD patients with current dehydration was significant lower (p < 0.001). CONCLUSION: Increased osmolarity level was associated with increased risk of DKD and elevated risk of all-cause mortality in DKD patients.

Suppressing scattering-induced nanosecond pre-pulses in Ti:sapphire multi-pass amplifiers.

In this Letter, we experimentally investigate a new kind of nanosecond pre-pulse, which originates from the bidirectional scattering of crystals in traditional Ti:sapphire multi-pass amplifiers. The experimental results demonstrate that the intensity of scattering-induced pre-pulses is very sensitive to the scattering angle, and the delay time between the pre-pulse and the main pulse is an integer multiple of the light path in each pass of the amplifier. An optimized multi-pass amplifier configuration is proposed, for what is believed to be the first time, to suppress the scattering-induced pre-pulses. The contrast ratio between pre-pulses and the main pulse is enhanced by more than two orders of magnitude, reaching a level of 10-10. This novel multi-pass amplifier configuration is very simple and economical, and provides an effective solution for the temporal contrast enhancement in the nanosecond range.

Simultaneous measurement of the absolute and relative time delay of a tiled-aperture coherent beam combination via the double-humped spectral beam interferometry.

Coherent beam combination (CBC) is a promising technology for achieving several hundred petawatts and even EW-level lasers. However, the measurement of the synchronization error and the time jitter of CBC is one of key technical issues, especially in the few-cycle PW-level laser facilities. In this paper, we demonstrate that the absolute time delay (ATD) and the relative time delay (RTD) for a tiled-aperture CBC can simultaneously be measured by using the double-humped spectral beam interferometry. The experimental study also was demonstrated. A root-mean-square deviation of approximately λ/38 (70 as) and a combining efficiency of 87.3% at 1 Hz closed feedback loop was obtained, respectively. Due to the wide adjustment range and a vast resisting beam energy disturbance capacity, this technique provide an effective and practical solution for measuring simultaneously the ATD and the RTD in the few-cycle PW-level laser pulses CBC.

Method for evaluating ophthalmic lens based on Eye-Lens-Object optical system.

We propose an evaluation method to judge the fitting extent to ophthalmic lens for the individual wearer. An Eye-Lens-Object optical system is set according to wearer's visual performance and the characteristic of ophthalmic lens assembly. A visual reference surface is proposed to calculate the object distance. The RMS radius of the spot diagram and MTF average value from optical design software Zemax are regarded as the criterion of assessing the image quality on the retina. Three cases are simulated to verify that our method is effective. The wearers can experience a comfortable wearing feeling when the evaluation method is used during the design of ophthalmic lens. The validity of our method is demonstrated to instruct designing the progressive addition lens with the freeform surface.

339 J high-energy Ti:sapphire chirped-pulse amplifier for 10 PW laser facility.

We report on the laser pulse output of 339 J centered at 800 nm from a chirped-pulse amplification (CPA) Ti:sapphire laser system at the Shanghai Superintense Ultrafast Laser Facility. The experimental results demonstrated that the parasitic lasing as well as the transverse amplified spontaneous emission of the homemade 235-mm-diameter Ti:sapphire final amplifier were suppressed successfully via the temporal dual-pulse pumped scheme and the index-matching liquid cladding technique. The maximum pump-to-signal conversion efficiency of 32.1% was measured for the final amplifier. With a compressor transmission efficiency of 64% and a compressed pulse duration of 21 fs obtained for the sample light at a lower energy level, this laser system could potentially generate a compressed laser pulse with a peak power of 10.3 PW. The experimental results represent significant progress with respect to the CPA laser.

Role of reactive oxygen species in high concentration glucose-induced growth inhibition of human peritoneal mesothelial cells.

Background: To investigate the effects and mechanism of high concentration glucose (HG), exogenous hydrogen peroxide (H2O2), and antioxidants on the cell growth (cell proliferation) of human peritoneal mesothelial cells (HPMCs). Methods: All tests were conducted on cultured HPMCs (HMrSV5) in vitro. Various concentrations of glucose (0.1%, 1.35%, and 3.86%), H2O2 (0.5 and 0.1 mmol/L), and antioxidants (pyruvate and catalase) were used in cell culture. Moreover, in order to study the interaction between these factors, HG and H2O2, HG and antioxidants, HG, H2O2, and antioxidants, were used respectively. After 12-24 h, phase-contrast microscopy was used to examine the morphological changes of HPMCs. DNA synthesis was detected by 3H-thymidine incorporation to measure cell proliferation, and flow cytometry was used to evaluate the proportion of cells in G1 phase. Furthermore, semiquantitative reverse-transcription polymerase chain reaction (RT-PCR) was utilized to determine the mRNA expression of p21Waf1 and p27Kip1 [cyclin-dependent kinase inhibitors (CKIs)], while immunocytochemistry (ICC) and Western blotting were employed to measure the protein expression of p21Waf1 and p27Kip1. Results: HG or low-dose exogenous H2O2 resulted in hypertrophy and senescence of HPMCs, resulting in similar morphological changes. Both HG and exogenous H2O2 (0.5 mmol/L) inhibited the proliferation of HPMCs and led to G1 phase arrest of HPMCs. The proportion of cells in G1 phase increased. Moreover, HG enhanced the toxic effects of exogenous H2O2. Both HG and exogenous H2O2 increased the expression of p21Waf1 and p27Kip1 . The addition of an antioxidant in HG medium arrested cells in the G1 phase and improved the inhibited cell proliferation. Conclusions: Both HG and exogenous H2O2 treatments can induce growth inhibition of HPMCs by arresting cell cycle progression, which is partially due to the increased expression of p21Waf1 and p27Kip1 . Thus, the effects of HG might be associated with endogenous reactive oxygen species (ROS), and it might be beneficial to use antioxidants in peritoneal dialysis (PD).

Effect of nonoperative treatment on the outcome of patients with posttraumatic hydrocephalus.

OBJECTIVE: To compare the outcome of non-operative treatment with the outcome of surgical intraventricular drainage for patients with posttraumatic hydrocephalus including complications, mortality and favorable outcome. METHODS: Thirty-nine patients were assigned to a nonoperative treatment group and 38 patents to a surgical intraventricular drainage group. Each patient's outcome was evaluated 3 years after treatment by using Glasgow Outcome Scale. RESULTS: In the nonoperative treatment group the rate of favor able outcome (good recovery or mild disability) was 89.74% (35 of 39 patients) and the mortality was 2.56% (1 of 39 patients). In the surgical intraventricular drainage group the rate of favorable outcome was 71.05% (27 of 38 patients) and the mortality was 13.16% (5 of 38 patients; P<0.05). Mortality was not significantly different between the two groups. CONCLUSIONS: Nonoperative treatment may be better than surgical intraventricular drainage for patients with posttraumatic hydrocephalus.

Investigation of electronic properties of graphene/Si field-effect transistor.

We report a high-performance graphene/Si field-effect transistor fabricated via rapid chemical vapor deposition. Oligolayered graphene with a large uniform surface acts as the local gate of the graphene transistors. The scaled transconductance, gm, of the graphene transistors exceeds 3 mS/µm, and the ratio of the current switch, Ion/Ioff, is up to 100. Moreover, the output properties of the graphene transistor show significant current saturation, and the graphene transistor can be modulated using the local graphene gate. These results clearly show that the device is well suited for analog applications.