RESUMO
The aim of this study was to evaluate a possible relationship between oxidative stress and transforming growth factor beta 1 (TGF beta 1) expression in human colon adenocarcinoma. Crohn's disease, an inflammatory pathology of the intestine often regarded to as precancerous, was also examined. Indices of impaired redox balance were monitored in blood and in bioptic samples from 10 adult patients with adenocarcinoma of the colon and from five patients with Crohn's disease. On tissue samples TGF beta 1 mRNA expression was also determined. Ten healthy adults provided normal reference values for plasma indices of oxidative stress, and normal tissue distant from the lesions was used for comparative analysis. Fluorescent adducts with plasma proteins of malonaldehyde (MDA) and 4-hydroxynonenal (HNE) were significantly lower than controls in the plasma from cancer patients and significantly higher in the plasma from Crohn's patients. In adenocarcinoma biopsies, susceptibility to lipid peroxidation processes and TGF beta 1 expression were below the relative control; in Crohn's disease, lipid peroxidation and cytokine expression were both above the relative control. The findings obtained suggest the existence of an association between oxidative damage and fibrogenic cytokine expression in the human intestine. Further studies are needed to conclusively prove the correlation between the two events.
Assuntos
Adenocarcinoma/metabolismo , Neoplasias do Colo/metabolismo , Estresse Oxidativo , Lesões Pré-Cancerosas/metabolismo , Fator de Crescimento Transformador beta/genética , Adenocarcinoma/genética , Adulto , Idoso , Idoso de 80 Anos ou mais , Estudos de Casos e Controles , Neoplasias do Colo/genética , Doença de Crohn/genética , Doença de Crohn/metabolismo , Feminino , Expressão Gênica , Humanos , Masculino , Malondialdeído/metabolismo , Pessoa de Meia-Idade , Lesões Pré-Cancerosas/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Neoplásico/genética , RNA Neoplásico/metabolismoRESUMO
Modern methods of semeiological detection of bronchiogenic cancer are discussed in the light of experience gained in a hospital chest surgery centre. Emphasis is laid on the advantages offered by new advances in nuclear diagnosis and bronchial endoscopy. The limitations of exploratory thoractomy are explained. Attention is drawn to the by no means inconsiderable number of cases in which early knowledge of the nature of the process could not be obtained. A series of illustrative cases are presented.