RESUMO
The discovery of new drugs to treat pathological cells in the case of aggressive liver primary cancer is imposing the identification of high-throughput screening systems to predict the in vivo response of new therapeutic molecules, in order to reduce current use of animals and drug testing costs. Recently, micro/nanostructured scaffolds have been adopted to reproduce the hepatic microenvironment due to their higher similarity to the biological niche with respect to the traditional two-dimensional culture plate, so providing novel in vitro models for reliably understanding molecular mechanisms related to cancer cells activity. Herein, we propose the study of electrospun scaffolds made of polycaprolactone as in vitro model that can mimic the morphological organization of native extracellular matrix and the co-culture of hepatic cell lines-i.e., HepG2, human healthy hepatocytes (HHH). The micro- and nano-scale morphological features of fibers with diameter equal to (3.22 ± 0.42) µm and surface roughness of (17.84 ± 4.43) nm-allow the reproduction of the in vivo scenario influencing the adhesion and proliferation rate of the cultured cells. A much lower proliferation rate is observed for the HepG2 cells compared to the HHH cells, when cultured on the fibrous scaffolds over a time course of 4 weeks. Moreover, results on oxidative stress mechanisms indicate an antioxidant effect of fibers mainly in the case of co-colture, thus suggesting a promising use as new in vitro models to explore alternative therapeutic strategies in hepatocarcinoma treatment.
Assuntos
Matriz Extracelular/química , Hepatócitos/citologia , Engenharia Tecidual/métodos , Alicerces Teciduais/química , Bromodesoxiuridina/química , Linhagem Celular , Proliferação de Células , Técnicas de Cocultura , Células Hep G2 , Humanos , Processamento de Imagem Assistida por Computador/métodos , Fígado/cirurgia , Poliésteres/química , Espécies Reativas de Oxigênio/metabolismoRESUMO
The relationship between unilateral posterior cross-bite and area and volumetric proportions of the palatal vault observed in the frontal plane were studied in 6 cross-bite cases before and after the correction of the malocclusion by removable appliances. Comparison of area and volume were carried out between the hemipalatal vault with the teeth in cross-bite and the hemipalatal vault with the teeth in good occlusion. The results demonstrated discrepancies of measurements between the two hemipalatal vaults before treatment. After the correction of cross-bite few or no asymmetries were recorded.