A high-level ab initio study of the photodissociation of acetaldehyde.

Acetaldehyde is a very relevant atmospheric species whose photodissociation has been extensively studied in the first absorption band both experimentally and theoretically. Very few works have been reported on acetaldehyde photodissociation at higher excitation energies. In this work, the photodissociation dynamics of acetaldehyde is investigated by means of high-level multireference configuration interaction ab initio calculations. Five different fragmentation pathways of acetaldehyde are explored by calculating the potential-energy curves of the ground and several excited electronic states along the corresponding dissociating bond distances. The excitation energy range covered in the study is up to 10 eV, nearly the ionization energy of acetaldehyde. We intend to rationalize the available experimental results and, in particular, to elucidate why some of the studied fragmentation pathways are experimentally observed in the different excitation energy regions and some others are not. Based on the shape of the calculated potential curves, we are able to explain the main findings of the available experiments, also suggesting possible dynamical dissociation mechanisms in the different energy regions. Thus, the reported potential curves are envisioned as a useful tool to interpret the currently available experiments as well as future ones on acetaldehyde photodissociation at excitation wavelengths in the range studied here.

Photodissociation of the CH2Br radical: A theoretical study.

Bromine atom (Br) reactions lead to ozone depletion in the troposphere and stratosphere. Photodegradation of bromocarbons is one of the main sources of bromine atoms in the atmosphere. Here, we use high-level ab initio methods, including spin-orbit effects, to study the photodissociation of the CH2Br radical. All possible fragmentation pathways, namely CH2Br + hν → CH2 + Br, HCBr + H, and CBr + H2, have been analyzed. Potential-energy curves of the ground and several excited electronic states along the corresponding dissociating bond distance of each pathway have been calculated. Considering the actinic fluxes of solar irradiation in the troposphere and in the stratosphere in the relevant range of frequencies, it is found that the first five excited states of CH2Br can be accessed from the ground state. Analysis of the potential curves shows that the pathways producing CH2 + Br and HCBr + H can proceed through a fast direct dissociation mechanism, while the pathway leading to CBr + H2 involves much slower dissociation mechanisms like internal conversion between electronic states, predissociation, or tunneling through exit barriers. The main implications are that the two faster channels are predicted to be dominant, and the slower pathway is expected to be less relevant. The tropospheric and stratospheric solar actinic fluxes also allow for further dissociation of the HCBr and CBr fragments, generating additional Br atoms, provided that they survive possible collisions with other atmospheric reagents. Finally, we discuss the possible effect of each of the three CH2Br dissociation pathways on the depletion of atmospheric ozone.

An ab initio study of the photodissociation of CH2I and CH2I.

Photodissociation of the CH2I radical and the CH2I+ cation is studied by means of high-level ab initio calculations, including spin-orbit effects. Potential-energy curves (PEC) along the dissociating bond distances involved in some fragmentation pathways of these species are computed for the ground and several excited electronic states. Based on the PECs obtained, the possible photodissociation mechanisms are analyzed and suggested. Significant differences are found between the fragmentation dynamics of the neutral radical and that of the cation. While a relatively simple dissociation dynamics is predicted for CH2I, more complex fragmentation mechanisms involving internal conversion and couplings between different excited electronic states are expected for CH2I+. The species studied here are relevant to atmospheric chemistry, and the present work can help to understand better how their photodissociation may affect chemical processes in the atmosphere.

An ab initio study of the photodissociation of the vinyl radical.

Photodissociation of the vinyl radical through pathways CH2CH → CH2C + H, CH2CH → CHCH + H, and CH2CH → CH2 + CH is investigated by means of high-level ab initio calculations. Potential-energy curves (PECs) along the corresponding dissociating bond distance associated with the ground and several excited electronic states involved in the above fragmentation pathways, as well as the nonadiabatic couplings connecting the different states, are obtained. The findings of several experiments on vinyl photodissociation performed at different excitation wavelengths are analyzed and explained qualitatively in the light of the present PECs. A two-dimensional representation (consisting of radial and angular coordinates to represent one of the H atoms of the CH2 group) is also used to calculate the electronic states. The surfaces obtained reflect a rich variety of conical intersections, exit barriers, and nonadiabatic couplings leading to predissociation in different regions of energy and of the two coordinates, suggesting a complex photodissociation dynamics of the CH2CH → CHCH + H pathway, with rather different fragmentation mechanisms involved. The two-dimensional results also provide interesting information on the mechanism of in-plane hydrogen migration from the CH2 group to the CH one through a high-lying transition state.

Photodissociation of the methyl radical: the role of nonadiabatic couplings in enhancing the variety of dissociation mechanisms.

The nonadiabatic photodissociation dynamics of the CH3 (and CD3) radical from the 3pz and 3s Rydberg states is investigated by applying a one-dimensional (1D) wave packet model that uses recently calculated ab initio 1D electronic potential-energy curves and nonadiabatic couplings. Calculated predissociation lifetimes are found to be too long as compared to the experimental ones. The 1D dynamical model, however, is able to predict qualitatively and explain the fragmentation mechanisms that produce the hydrogen-fragment translational energy distributions (TED) measured experimentally for the ground vibrational resonance of the 3pz and 3s Rydberg states (CH3(v = 0, 3pz) and CH3(v = 0, 3s)). The CH3(v = 0, 3pz) TED found experimentally displays a rather large energy spreading, while the experimental CH3(v = 0, 3s) TED is remarkably more localized in energy. The present model also predicts a widely spread CH3(v = 0, 3pz) TED, produced by a complex dissociation mechanism which involves predissociation to one dissociative valence state through a nonadiabatic coupling, as well as transfer of population to a second valence state through three conical intersections. Also in agreement with experiment, the model predicts a rather localized CH3(v = 0, 3s) TED because the conical intersections no longer operate in this photodissociation process, and predissociation occurs only into a single valence state. Another complex dissociation mechanism is predicted by the model for initial CH3(v > 0, 3s) and CD3(v > 0, 3s) resonances. In this case the mechanism is gradually activated, as vibrational excitation increases, by the interplay between the two nonadiabatic couplings connecting the 3s and 3px,y Rydberg states with the dissociative 2A1 valence state, and produces complex TEDs with signals from several resonances of both 3s and 3px,y. Thus the present 1D quantum model reveals a rich photodissociation dynamics of methyl, where a variety of complex fragmentation mechanisms is favored by the presence of different nonadiabatic couplings between the electronic states involved.

Interference of a resonance state with itself: a route to control its dynamical behaviour.

It is demonstrated both numerically and mathematically that the dynamical behavior of an isolated resonance state, which comprises the resonance decay lifetime and the asymptotic fragment state distribution produced upon resonance decay, can be extensively controlled by means of quantum interference induced by a laser field in the weak-field regime. The control scheme applied is designed to induce interference between amplitudes excited at two different energies of the resonance line shape, namely the resonance energy and an additional energy. This scheme exploits the resonance property of possessing a nonzero energy width, which makes it possible that a resonance state may interfere with itself, and thus allows interference between the amplitudes excited at the two energies of the resonance width. The application of this scheme opens the possibility of a universal control of both the duration and the fragment product distribution outcome of any resonance-mediated molecular process.

A unified theory of weak-field coherent control of the behavior of a resonance state.

A unified weak-field control scheme to modify the two properties that determine the whole behavior of a resonance state, namely the lifetime and the asymptotic fragment distribution produced upon resonance decay, is proposed. Control is exerted through quantum interference induced between overlapping resonances of the system, by exciting two different energies at which the resonances overlap. The scheme applies a laser field consisting of a first pulse that excites the energy of the resonance to be controlled, and two additional pulses that excite another different energy to induce interference, with a delay time with respect to the first pulse. Each of the two additional pulses is used to control one of the two resonance properties, by adjusting its corresponding delay time: with a relatively short delay time the second pulse controls the resonance lifetime, while with a very long delay time the third pulse modifies the asymptotic fragment distribution produced. The efficiency of the control of each resonance property is found to be strongly dependent on the choice of the second interfering energy, which allows for a more flexible control optimization by choosing a different energy for each property. The theory underlying the interference mechanism of the control scheme is developed and presented, and is applied to analyze and explain the results obtained. The present scheme thus appears to be a useful tool for controlling resonance-mediated molecular processes.

Weak-field coherent control of photodissociation in polyatomic molecules.

A coherent control scheme is suggested to modify the output of photodissociation in a polyatomic system. The performance of the scheme is illustrated by applying it to the ultrafast photodissociation of CH3I in the A-band. The control scheme uses a pump laser weak field that combines two pulses of a few femtoseconds delayed in time. By varying the time delay between the pulses, the shape of the laser field spectral profile is modulated, which causes a change in the initial relative populations excited by the pump laser to the different electronic states involved in the photodissociation. Such a change in the relative populations produces different photodissociation outputs, which is the basis of the control achieved. The degree of control obtained over different photodissociation observables, like the branching ratio between the two dissociation channels of CH3I yielding I(2P3/2) and I*(2P1/2) and the fragment angular distributions associated with each channel, is investigated. These magnitudes are found to oscillate strongly with the time delay, with the branching ratio changing by factors between two and three. Substantial variations of the angular distributions also indicate that the scheme provides a high degree of control. Experimental application of the scheme to general polyatomic photodissociation processes should be straightforward.

Delaying the Decay of a Superposition of Resonance States.

A weak-field coherent control scheme is applied in order to enhance the decay lifetime of a superposition of overlapping resonance states. The scheme uses a pump laser field consisting of two pulses delayed in time, each of them exciting a different energy at which several resonances of the Ne-Br2(B) complex overlap. Simultaneous excitation of these two energies induces interference between the overlapping resonances, which causes an enhancement of the lifetime of the superposition created. By variation of the delay time between the pulses, the mechanism of resonance interference can be controlled and optimized to achieve a maximum lifetime enhancement. The optimal delay time between pulses leading to maximum superposition lifetime can be quantitatively predicted with a simple law. The effect of the interference mechanism on the lifetime enhancement is investigated. It is found that interference induces a transfer of amplitude between the different resonances back and forth, which delays significantly the natural resonance decay, increasing the global lifetime of the superposition. Due to the simplicity of the control scheme, a wide applicability is envisioned.

Thyroid hormone resistance from newborns to adults: a Spanish experience.

OBJECTIVE: Thyroid hormone resistance (RTH ß) is a rare genetic disorder characterized by an altered response of target tissue to the action of thyroid hormone. Few studies on RTH ß have been carried out in southern European populations. We aimed to describe the clinical and genetic characteristics at the time of diagnosis in a Spanish cohort of patients with genetically confirmed RTH ß, with ages ranging from newborns to adults. METHODS: Retrospective multicenter study of 28 patients who were genetically confirmed as RTH ß. Clinical and biochemical data were collected from the reference centers, and the studied variables included age, sex, anthropometric data, clinical characteristics and biochemical results. In the Basque country, a simultaneous analysis of TSH and T4 is carried out in the program for the screening of inborn errors of metabolism. A molecular analysis of the thyroid hormone beta (THRB) gene was performed. RESULTS: The total cohort included 20 adults and eight pediatric patients (six newborns). Of the total, 5 (17.8%) were diagnosed by clinical characteristics (goiter, hypertension or tachycardia), 13 (46.4%) were analyzed in the context of a family study and 10 (35.7%) were diagnosed after obtaining an altered fT4 and/or TSH level in a biochemical analysis performed due to clinical symptoms unrelated to RTH ß. Four of the newborns included in the series were diagnosed by the result of neonatal screening, which allows us to estimate a minimum local incidence of RTH ß of 1/18,750 live newborns. The genetic analysis showed the presence of 12 different heterozygous mutations in the THRB gene. CONCLUSIONS: We report the clinical and genetic characteristics of a Spanish RTH ß cohort, from neonates to adults. We also describe one novel mutation in the THRB gene as the cause of the disease. The simultaneous analysis of TSH and T4 carried out in the program for the screening of inborn errors of metabolism facilitates the early diagnosis of RTH ß in newborns and has allowed us to estimate a minimum local incidence of RTH of 1/18,750 live newborns.

Weak-Field Coherent Control of Molecular Photofragment State Distributions.

It is known that the long-time energy-resolved photofragment state distribution produced upon photodissociation of a molecule cannot be modified in the weak-field limit for a fixed pump pulse spectral profile. This work, however, demonstrates both computationally and mathematically that the above limitation can be circumvented in practice when the molecule presents overlapping resonances. It is shown that when two or more energies where the resonances overlap are excited by different laser pulses delayed in time, interference is induced between the product fragment states associated with the different energies populated. The occurrence of interference is found to be independent of the delay time between the pulses exciting the different energies. Thus, as demonstrated, this finding makes it possible to modify the fragment distribution at a given energy, as far in time and as many times as desired.

Unravelling the mechanisms of interference between overlapping resonances.

The enhancement of the resonance lifetime that occurs upon interference of two overlapping resonances excited coherently by two pulses with delayed time has been investigated as a function of the pulse temporal width and the delay time between the pulses. A general law predicting quantitatively the optimal delay time that maximizes the lifetime enhancement of the two resonances has been established in terms of the pulse width and of the lifetimes of both resonances when they are excited isolatedly. The specific form of the law and all the results found can be closely related to the characteristic features of the mechanism of interference between the overlapping resonances, providing a detailed understanding on how the mechanism operates. The proposed law is envisioned as a useful tool to design experimental strategies to control the resonance lifetime.

Limited performance of MALDI-TOF for identification of fish Aeromonas isolates at species level.

The aim of this study was to evaluate the usefulness of the MALDI-TOF MS to identify 151 isolates of Aeromonas obtained mostly from diseased fish. MALDI-TOF MS correctly identified all isolates to the genus level but important differences in the percentage of isolates correctly identified depending on the species were observed. Considering exclusively the first identification option, Aeromonas bestiarum, Aeromonas hydrophila, Aeromonas salmonicida, Aeromonas veronii and Aeromonas sobria were the best identified with results >95%. However, considering the first and second identification options, the only species that showed values >90% was A. hydrophila. Overall, when the database was supplemented with 14 new spectra, the number of accurate identifications increased (41% vs. 55%) and the number of inconclusive identifications decreased (45% vs. 29%), but great differences in the success of species-level identifications were found. Species-distinctive mass peaks were identified only for A. hydrophila and A. bestiarum (5003 and 7360 m/z in 95.5% and 94.1% of their isolates, respectively). This work demonstrates the utility of MALDI-TOF MS for Aeromonas identification to the genus level, but there is no consistency for the accurate identification of some of the most prevalent species implicated in fish disease.

Photodissociation of the CH3O and CH3S radical molecules: an ab initio electronic structure study.

The electronic states and the spin-orbit couplings between them involved in the photodissociation process of the radical molecules CH3X, CH3X → CH3 + X (X = O, S), taking place after the Ã(2A1) ← X[combining tilde](2E) transition, have been investigated using highly correlated ab initio techniques. A two-dimensional representation of both the potential-energy surfaces (PESs) and the couplings is generated. This description includes the C-X dissociative mode and the CH3 umbrella mode. Spin-orbit effects are found to play a relevant role in the shape of the excited state potential-energy surfaces, particularly in the CH3S case where the spin-orbit couplings are more than twice more intense than in CH3O. The potential surfaces and couplings reported here for the present set of electronic states allow for the first complete description of the above photodissociation process. The different photodissociation mechanisms are analyzed and discussed in light of the results obtained.

Increased expression of microRNA-155 in peripheral blood mononuclear cells from psoriasis patients is related to disease activity.

BACKGROUND: MicroRNAs (miRNAs) gene expression regulators are altered in psoriasis suggesting their role in the pathogenesis. OBJECTIVE: To study expression changes of inflammation and toll-like receptor (TLR)-related miRNAs, miRNA-155, let-7i, miRNA-21, miRNA-146a and miRNA-223 in peripheral mononuclear cells (PBMCs) and miRNA-21, miRNA-146a and miRNA-223 in plasma, from chronic plaque-type psoriasis patients who were treatment-naive or had undergone a washout period (n = 11). MiRNAs were evaluated at baseline and after 11 (9-12) months [median (25th-75th percentile range)] of methotrexate (MTX) or topical (betamethasone plus calcipotriene) treatment. METHODS: MiRNA expression was analysed with quantitative real-time reverse transcription-polymerase chain reaction. Matched controls were studied. RESULTS: Psoriasis patients presented, at baseline, increased expression of miRNA-155, let-7i, miRNA-146a, miRNA-21 and miRNA-223 in PBMCs, plus miRNA-21, miRNA-146a and miRNA-223 in plasma. Receiver-operator characteristic (ROC) curve analysis and area under the curve (AUC) showed that expression of these miRNAs have the potential to distinguish between psoriasis and controls. At baseline, miRNA-155 expression in PBMCs correlated with Psoriasis Area Severity Index (PASI) [12 (8-14)] (Spearman r: 0.7140, P < 0.05) suggesting a role in psoriasis. After MTX or topical treatment, reduction in PASI was observed [87.5% (75-100)]; miRNA-155 expression in PBMCs decreased; plasma miRNA-21, miRNA-146a and miRNA-223 were down-regulated. ROC analysis showed that miRNA-155 expression in PBMCs from psoriasis patients have the potential to distinguish between patients' samples at baseline and after treatment (AUC: 0.942, sensitivity: 0.91; specificity: 0.91 values; maximum likelihood ratio =10). After treatment, miRNA-146a expression in PBMCs increased; miRNA-155/miRNA-146a ratio decreased, suggestive of a regulatory feedback; let-7i expression decreased; miRNA-21 and miRNA-223 remained elevated. CONCLUSION: In this exploratory study, psoriasis patients presented increased expression of miRNA-155 in PBMCs that correlated with PASI and decreased with disease remission. MiRNA-21, miRNA-146a and miRNA-223 in PBMCs and plasma were increased at baseline and differentially modulated, underscoring different roles of TLR-related miRNAs in psoriasis.

Coinfection by Streptococcus phocae and cetacean morbillivirus in a short-beaked common dolphin Delphinus delphis.

We describe gross, histopathological, and immunohistochemical features of Streptococcus phocae and cetacean morbillivirus coinfection in a short-beaked common dolphin Delphinus delphis. Major gross findings were cutaneous purulent nodules in the tail fluke, vegetative mitral valve endocarditis, and presumed postpartum pyometra. Histologic examination revealed bacterial septicemia characterized by widespread intravascular coccoid bacterial emboli. These were associated with fibrinonecrotizing to pyogranulomatous dermatitis and panniculitis, embolic pneumonia, neutrophilic and lymphoplasmacytic meningochoroiditis, random neutrophilic hepatitis, lymphoplasmacytic myocarditis and epicarditis, necrotizing adrenalitis, suppurative endometritis, and multicentric reactive lymphadenopathy. Bacteriology and molecular analysis with sequencing of the 16S rRNA gene identified S. phocae from lung, brain, and adrenal gland tissue. Immunohistochemical analysis for morbillivirus detection revealed positive immunolabeling in the epithelium of the choroid plexus of the fourth ventricle. Published reports on S. phocae infection in cetaceans are rare, and pathological details are limited. The present case indicates that S. phocae has potential pathogenic capacity in common dolphins. The pathogenesis is proposed to have involved cutaneous penetration after a skin trauma, leading to initial cutaneous disease and eventual systemic infection.

Assessing the effect of preoperative levosimendan on renal function in patients with right ventricular dysfunction.

The Acute Kidney Injury Network (AKIN) classification considers SCr values, urea and urine output in order to improve timely diagnose ARF and improve patient prognosis by early treatment. Preoperative levosimendan is a new way for cardiac and kidney protection, we try to evaluate this drug in fifteen patients comparing values of AKIN scale parameters pre and post cardiac surgery in patients with right ventricle dysfunction.

Streptococcus caprae sp. nov., isolated from Iberian ibex (Capra pyrenaica hispanica).

Biochemical and molecular genetic studies were performed on a novel Gram-stain-positive, catalase-negative, coccus-shaped organism isolated from tonsil samples of two Iberian ibexes. The micro-organism was identified as a streptococcal species based on its cellular, morphological and biochemical characteristics. 16S rRNA gene sequence comparison studies confirmed its identification as a member of the genus Streptococcus, but the organism did not correspond to any species of this genus. The nearest phylogenetic relative of the unknown coccus from ibex was Streptococcus porci 2923-03T (96.6 % 16S rRNA gene sequence similarity). Analysis based on rpoB and sodA gene sequences revealed sequence similarity values lower than 86.0 and 83.8 %, respectively, from the type strains of recognized Streptococcus species. The novel bacterial isolate was distinguished from Streptococcus porci and other Streptococcus species using biochemical tests. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as representing a novel species of the genus Streptococcus, for which the name Streptococcus caprae sp. nov. is proposed. The type strain is DICM07-02790-1CT ( = CECT 8872T = CCUG 67170T).

Weak-field laser phase modulation coherent control of asymptotic photofragment distributions.

Coherent control of the asymptotic photofragment state-resolved distributions by means of laser phase modulation in the weak-field limit is demonstrated computationally for a polyatomic molecule. The control scheme proposed applies a pump laser field consisting of two pulses delayed in time. Phase modulation of the spectral bandwidth profile of the laser field is achieved by varying the time delay between the pulses. The underlying equations show that such a phase modulation is effective in order to produce control effects on the asymptotic, long-time limit photofragment distributions only when the bandwidths of the two pulses overlap in a frequency range. The frequency overlap of the pulses gives rise to an interference term which is responsible for the modulation of the spectral profile shape. The magnitude of the range of spectral overlap between the pulses becomes an additional control parameter. The control scheme is illustrated computationally for the asymptotic photofragment state distributions produced from different scenarios of the Ne-Br2 predissociation. An experimental application of the control scheme is found to be straightforward.

An ab initio study of the ground and excited electronic states of the methyl radical.

The ground and some excited electronic states of the methyl radical have been characterized by means of highly correlated ab intio techniques. The specific excited states investigated are those involved in the dissociation of the radical, namely the 3s and 3pz Rydberg states, and the A1 and B1 valence states crossing them, respectively. The C-H dissociative coordinate and the HCH bending angle were considered in order to generate the first two-dimensional ab initio representation of the potential surfaces of the above electronic states of CH3, along with the nonadiabatic couplings between them. Spectroscopic constants and frequencies calculated for the ground and bound excited states agree well with most of the available experimental data. Implications of the shape of the excited potential surfaces and couplings for the dissociation pathways of CH3 are discussed in the light of recent experimental results for dissociation from low-lying vibrational states of CH3. Based on the ab initio data some predictions are made regarding methyl photodissociation from higher initial vibrational states.