Mapping of the Retrotransposable Elements Rex1 and Rex3 in Chromosomes of Eigenmannia (Teleostei, Gymnotiformes, Sternopygidae).

Transposable elements constitute a remarkable fraction of the eukaryote genome and show particular capacity to move and insert in specific regions of the genome. This study identified the retrotransposable elements Rex1 and Rex3 in the genomes of 6 cytotypes of Eigenmannia. The sequences were isolated by PCR, sequenced and physically mapped in the chromosomes of these cytotypes, aiming to investigate the organization and distribution of these elements in this fish group, mainly in the sex chromosomes. The FISH physical mapping revealed that both Rex1 and Rex3 elements are dispersed in small clusters throughout the chromosomes of all cytotypes analyzed. However, conspicuous blocks occur in several samples, including an accentuated accumulation of the Rex3 element in X(1) and X(2) chromosomes of Eigenmannia sp. 2 and in the X chromosome of E. virescens. The accumulations are coincident with heterochromatin-rich regions, suggesting that Rex3 played a role in the differentiation process of the sex chromosomes.

[Anaphylaxis to blue dyes]. / Anaphylaxie aux colorants bleus.

In medicine, vital blue dyes are mainly used for the evaluation of sentinel lymph nodes in oncologic surgery. Perioperative anaphylaxis to blue dyes is a rare but significant complication. Allergic reactions to blue dyes are supposedly IgE-mediated and mainly caused by triarylmethanes (patent blue and isosulfane blue) and less frequently by methylene blue. These substances usually do not feature on the anesthesia record and should not be omitted from the list of suspects having caused the perioperative reaction, in the same manner as latex and chlorhexidine. The diagnosis of hypersensitivity to vital blue dyes can be established by skin test. We illustrate this topic with three clinical cases.

Diagnosis and Management of Intravenous Drug Users' Chronic Wounds: A Case Series Collection of a Single Center.

Background: Intravenous drug users (IVDUs) represent a very small group of patients affected by chronic wounds (CWs). Objectives: To assess the risk factors for CWs in IVDUs, to improve their treatment. Methods: A retrospective review of 7 IVDUs with CWs was performed at the Dermatology of the University of Bologna. Results: A history of 10 years of the most frequent injection was heroin in the gaiter area. The most observed comorbidities were HIV, HCV and HBV. They were treated most with an alginate with silver dressing with an average follow-up of 2.8 years. Conclusions: We would like to highlight the importance of clinical history in this type of patients and that in our experience specialized skin wounds risk assessment and management could be useful, aside from traditional compression therapy.

The Mugil curema species complex (Pisces, Mugilidae): a new karyotype for the Pacific white mullet mitochondrial lineage.

Recent molecular phylogenetic analyses have shown that the Mugil curema Valenciennes, 1836 species complex includes M. incilis Hancock, 1830, M. thoburni (Jordan & Starks, 1896) and at least four "M. curema" mitochondrial lineages, considered as cryptic species. The cytogenetic data on some representatives of the species complex have shown a high cytogenetic diversity. This research reports the results of cytogenetic and molecular analyses of white mullet collected in Ecuador. The analyzed specimens were molecularly assigned to the Mugil sp. O, the putative cryptic species present in the Pacific Ocean and showed a 2n = 46 karyotype, which is composed of 2 metacentric and 44 subtelocentric/acrocentric chromosomes. This karyotype is different from the one described for M. incilis (2n = 48) and from those of the two western Atlantic lineages Mugil curema (2n = 28), and Mugil margaritae (2n = 24). Data suggest the need for a morphological analysis to assign a species name to this Pacific lineage.

The somatotype of medium class Italian basketball players.

The aim of this observational study was to determine the somatotype of average basketball players (BP) and to compare them with the values found in relevant literature. Thirty-eight Italian members of "B" and "C" BP league teams were measured according to the Health/Carter anthropometric somatotype method. Since our group was, on average, made up of mesomorphic-ectomorphs (2.2-3.2-3.8), it appeared to be quite well suited to the sporting activity undertaken. However, the comparison carried out for the anthropometric variables and the somatotype scores found for higher level athletes, show that their physique lacks some of the characteristics needed to excel.

The somatotype in a group of "top" free-climbers.

Thirty-one "top" level european free-climbers (FC) aged 26.1 +/- 4.3 years old, have been measured according to the Health/Carter anthropometric somatotype method, in order to collect data on the morphotypes needed to excel in this not well established sporting activity. The average somatotype for FC revealed to be: mesomorph-ectomorph (2.0 +/- 0.6; 4.0 +/- 0.8; 3.7 +/- 0.9), a facies found in the 54.8% of the performers. With their 8.3% FAT%, they fall inside the category of lean athletes, a well stated feature found in all those activities demanding endurance. When compared with 25 "sedentary" young italians aged 21.7 years old, FC showed to differ significantly in: weight, mean of the sum of the skinfolds, upper arm and geminal circumferences, RPI (reciprocal of the ponderal index), ectomorph component (less than .01). Concluding, their somatotype appeared to be similar to that of athletes whose fatness is lower, to some adolescent performers and to some extra-European cohorts of males living in stressing habitats.

Somatotype of Italian rugby players.

Our aim was to collect lacking first-hand data on Italian rugby players. The Heath/Carter anthropometric somatotype method was applied to 28 "A" League performers (RP) aged 25 +/- 3.9 years of age. Their somatotypes and dimensions were compared with those found in previous studies on athletes involved in the same sporting activity, with data collected on 25 "sedentary" young Italians, and with Bailey et al.'s study on Canadians (1982). On average, the RP group resulted as being endomorphic mesomorphs (3.1 +/- 1.1 - 5.6 +/- 1.3 - 1.4 +/- 1.1), a result that is congruent with international data. They differed significantly from the balanced mesomorph CG (2.3 +/- 1.0 - 4.5 +/- 1.2 - 2.5 +/- 1.4) for all the measurements taken, apart from bi-epycondylar width. The peculiar somatotype scores found are congruent with the needs of rugby, an aerobic-anaerobic discipline which requires performers with great muscular power associated with a capacity to furnish energy, mainly through the anaerobic metabolism.

The somatotype of "amateur" Italian female volleyball-players.

In order to collect data on the physique of "amateur" female volleyball players, 50 athletes (VP) were measured according to the Health/Carter anthropometric somatotype method. They were divided into two subgroups: juniors (JVP < 18 years old) and seniors (SVP > 18). Their somatotype scores were respectively: 4.9-3.8-2.6 and 4.7-3.9-2.3. No significant differences emerged for any of the components of the somatotype between the two subgroups. JVP and SVP resulted as being taller and stouter than the mean of the Italian girls of the same age, but their menstrual function parameters (such as: age at onset of menarche, cycle and flux lengths) did not differ. Centres were much taller and heavier than other players. For the whole VP sample, the values of anthropometric measures and somatotype scores resulted as being congruent with those ascertained for other cohorts of amateur VP found in relevant literature.

The morphotype in a group of peri-pubertal soccer players.

Few morphological data are available for adolescent soccer players (SP), a lack that could affect young-age talent selection. In order to collect lacking data for SP, 50 performers aged 13.1 +/- 0.5 years old were checked in one of the main "vivariums" of talented "A League" SP. All subjects were measured according to the Heath/Carter anthropometric somatotype method and subsequently, the results were compared with those found in relevant literature for adolescents. To gain further insight, the sample was subdivided into two classes, according to their performance level: beginners (B, n = 26) and those experienced (E, n = 24). Between B and E subjects significant differences were found for: weight, abdominal skinfold, calf circumference, body density (0.05 < p < 0.01); height, tricipital skinfold, humerus biepicondylar width, ponderal index, LBW, endo and ectomorph components (p < 0.01). B somatotype resulted to be: 1.6-4.3-3.5; while E: 2.2-4.5-2.9. Both in E and B sub-groups the distribution of somatotypes according to their component dominance revealed to be rather similar, indicating a substantial homogeneity inside the subgroups. When compared with adult SP, the whole sample shows a morphotype that is already adapted for soccer. Observing this, it is possible to infer that our adolescent group contains the morphotype needed to perform well (and probably to excel), in the considered sporting activity.

On the origins of human laterality: environmental and hereditary variables in a sample of children.

On the basis of operationally defined handedness data from 1,348 subjects of both sexes and of ages 10 to 15 years, laterality was compared with prehistorical data of handprints found in caves. It was hypothesized that the greater production of left handprints found in caves in comparison with current data should be ascribed to a nonestablished deviation of lefthanders towards righthandedness as environmental pressures in the distant past were lower.

Colour blindness in Italian art high school students.

To highlight the link between colour blindness and school achievement, the Ishihara and Farnsworth tests were administered to 3,565 high school art students (2,545 girls and 1,020 boys). Analysis showed colour defective students were discriminated against in theoretical subject matter, relative to orthochromate students, but not in the art-related subjects. This emphasizes the need to recognize youth with colour defective vision early.

Congenital dyschromatopsia and school achievement.

In 1993-94, in Liguria (a northwestern Italian region) a study was carried out on dyschromatopsia, a congenital sex-linked form of colour blindness. 3124 junior high school boys aged 10-15 years were tested using Ishihara plates (1973 edition) and Farnsworth's D-15 test (1947 edition). 152 students were identified as colour blind (4.87%), a value slightly below the Italian average of 5.3%. The school achievement of these students was assessed by means of the school marks of two randomised subsamples composed of 82 dyschromates and 82 orthochromates, paired homogeneously by age and class. Statistical analysis indicated significantly lower general school achievement for the 82 dyschromate subjects (except for art). The learning difficulties of dyschromate persons for whom colour is a basic didactic tool are discussed. Introduction of dyschromatopsia tests at preschool would be desirable.

Origin and distribution of Daltonism in Italy.

A research project was started in Italy in order to map dyschromatopsies in this country. Field data was collected by the authors in Liguria, Veneto, Emilia-Romagna, Apulia, Sicily, and by other authors in Lazio, Calabria, Basilicata, and Sardinia. The samples were all composed of more than 500 male subjects, of homogeneous origin, and assessed using the Ishihara (1973) and the Farnsworth (1947) tests. Statistical analysis identified two groups showing different mean percentages: one, coastal (n = 13,091; 6.50%) and the other, inland (n = 17,881; 5.21%). The results stimulated an ecological-ergonomic hypothesis based on nutrition (i.e., food gathering strategies) and the kind of light prevailing in the habitat. If trichromacy has been brought about by frugivore feeding (Polyach, 1957), dichromacy may have been maintained by giving a selective advantage to fishermen (originally fishing one fish at a time by hand). This is valid for both sea or fresh water habitats, characterized by blue-green prevailing light and with sharp discrimination for blue-gray shapes and colors (usually, 70% of dichromats are deutans). To support this hypothesis a field survey was carried out among 661 Sicilian traditional fishermen, which furnished the highest rates of dyschromatopsia in Italy: 7.90%. In conclusion, we can hypothesize that endogamous fishermen groups bordering the coastal areas of continents, searching for waters abounding in fish, could have determined a greater incidence of color blindness in the coastal regions, while exogamous marriages between fishermen and peasants contributed to the lower diffusion of the genes responsible in inland areas.

Polyhydroxynaphthalene reductase involved in melanin biosynthesis in Magnaporthe grisea. Purification, cDNA cloning and sequencing.

During the biosynthesis of fungal melanin, tetrahydroxynaphthalene reductase catalyzes the NADPH-dependent reduction of 1,3,6,8-tetrahydroxynaphthalene (T4HN) into (+)-scytalone and 1,3,8-trihydroxynaphthalene into (-)-vermelone. The enzyme from Magnaporthe grisea, the fungus responsible for rice blast disease, has been purified to homogeneity. It is a tetramer of four identical 30-kDa subunits. A full-length cDNA clone of about 1 kb encoding T4HN reductase has been isolated from a cDNA library constructed in the lambda ZAP II vector and characterized. The clone contains a 846-bp open reading frame. Translation of the DNA sequence gave a 282-residue amino acid sequence with a calculated molecular mass of 29.9 kDa. Sequences corresponding to the amino-terminal part and three internal proteolytic peptides were present in the translated sequence. T4HN reductase exhibits characteristics of the short-chain alcohol dehydrogenase family. The reductase shares 56% identity with a putative ketoreductase involved in aflatoxin biosynthesis in Aspergillus parasiticus.

Hypnosis in the treatment of anticipatory nausea and vomiting in patients receiving cancer chemotherapy.

AIMS AND BACKGROUND: In addition to nausea and vomiting following chemotherapy treatment, cancer patients can experience these side effects prior to a treatment session, the so-called anticipatory nausea and vomiting. As various psychological and neurophysiological aspects have been claimed to be implied in its etiopathogenesis, the present paper aims to shortly review the etiological, epidemiological and therapeutical assumptions on the topic, in particular the psychological-behavioral therapies. PATIENTS AND METHODS: The present study was carried out on 16 consecutive adult cancer patients affected by chemotherapy-induced anticipatory nausea and vomiting who had received at least four treatment cycles. All of them were submitted to induction of relaxation followed by hypnosis. RESULTS: In all subjects anticipatory nausea and vomiting disappeared, and major responses to chemotherapy-induced emesis control were recorded in almost all patients. CONCLUSIONS: The experience highlights the potential value of hypnosis in the management of anticipatory nausea and vomiting; furthermore, the susceptibility to anticipatory nausea and vomiting is discussed under the psychoanalytic point of view.

Mechanism of action and cloning of epoxide hydrolase from the cabbage looper, Trichoplusia ni.

The majority of the JH III epoxide hydrolase activity in last stadium day 3 (gate 1) wandering Trichoplusia ni was membrane bound with approximately 9% of the activity found in the cytosol. Both the microsomal and cytosolic JH epoxide hydrolases were stable, retaining 30% of their original activity after incubation at 4 degrees C for 15 days. 18O-labeled water underwent enzyme catalyzed regioselective addition to the least substituted C10 position of JH III. In multiple turnover reactions with JH epoxide hydrolase in 97.9% 18O-labeled water, only 91.3% 18O incorporation was observed. This is consistent with an SN2 reaction likely involving a carboxylate in the active site of JH epoxide hydrolase. The DNA amplification cloning of a fragment of a putative T. ni epoxide hydrolase is reported. The deduced amino acid sequence shares 67% similarity to the rat microsomal epoxide hydrolase.