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1.
J Invertebr Pathol ; 139: 50-55, 2016 09.
Artigo em Inglês | MEDLINE | ID: mdl-27418147

RESUMO

A qPCR assay specific for zoospores of Catenaria uncinata, a fungal parasite in eggs of the midge Glyptotendipes lobiferus, was developed and used in parallel with traditional microscopic methods in a season-long study of a C. uncinata/G. lobiferus association in a local pond. Twenty-six consecutive weekly collections of egg masses were screened with a microscope to obtain percentages of infection and mortality in organogenetic egg masses and weekly water samples were processed by absolute quantification using qPCR to obtain estimates of zoospore density. Overall, 36.0% of G. lobiferus egg masses were infected to varying degrees and 11.2% of eggs were killed by C. uncinata. Continuous infection of egg masses occurred during a 6-wk period in May-June and a 7-wk period in September-October. Infection by C. uncinata was absent during a 10-week interval between periods of infection. Abrupt declines in zoospore density occurred during both infection periods and occurred only when water temperatures met or exceeded the viability threshold for zoospores (⩾31.0°C). The episodic death of zoospores during weeks in which egg infection and mortality levels were continuous likely resulted from distribution of zoospores throughout the water column and a temperature gradient in which zoospores sampled near the surface were subjected to lethal temperatures while non-sampled zoospores at lower depths were provided low temperature sanctuary. The hiatus of infection during the 10-week interval was likely due to lethal temperatures throughout the water column as average water temperatures exceeded 31.0°C over the period. A positive correlation between weekly zoospore densities obtained from qPCR and levels of infection/mortality in egg masses obtained from counts with a microscope supports the use of the qPCR assay alone in future studies that can rapidly and accurately determine parasite presence, prevalence and geographical range.


Assuntos
Blastocladiomycota , Chironomidae/parasitologia , Interações Hospedeiro-Parasita/fisiologia , Micoses/veterinária , Animais , Microclima , Óvulo/parasitologia , Prevalência
3.
Mycologia ; 112(5): 989-1002, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32845791

RESUMO

This paper describes the taxonomy, developmental morphology, and phylogeny of Periplasma isogametum, a new monotypic member of the Leptomitales (Oomycota). In phylogenetic trees inferred from concatenated and separate nuc 18S rDNA (18S) and nuc 28S rDNA (28S) sequences, P. isogametum forms a well-supported clade related to but distinct from Apodachlya, a member of the Leptomitales. The organism is a holocarpic facultative saprotroph of moribund aquatic insects but grows well on a variety of mycological media in which it produces large eucarpic hyphae with a peripheral layer of protoplasm surrounding a central vacuole. In zoosporogenesis, a peripheral network of zoospore initials collapses to the center of the zoosporangium and is partitioned into individual heterokont zoospores. Sexual reproduction involves morphological isogamy in which male gametes produce elongate fertilization tubes, which fuse with female gametes, which are subsequently converted into thick-walled oospores. Developmental morphology is detailed in photomicrographs and pointillism drawings.


Assuntos
Insetos/microbiologia , Oomicetos/classificação , Oomicetos/citologia , Oomicetos/crescimento & desenvolvimento , Oomicetos/genética , Reprodução/genética , Reprodução/fisiologia , Animais , Organismos Aquáticos/classificação , Organismos Aquáticos/citologia , Organismos Aquáticos/genética , Organismos Aquáticos/crescimento & desenvolvimento , DNA Fúngico , Filogenia , RNA Ribossômico 18S , Análise de Sequência de DNA , Esporos Fúngicos/classificação , Esporos Fúngicos/citologia , Esporos Fúngicos/crescimento & desenvolvimento , Virginia
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