RESUMO
OBJECTIVES: The objective of the study was to determine the prevalence of HPV seropositivity among patients with oral squamous cell carcinoma (OSCC) and healthy individuals and to correlate the association between HPV 16 seropositivity and risk of OSCC. MATERIALS AND METHODS: HPV 16 E6 and E7 plasmids were constructed for the production of recombinant protein, which was used as the antigen in ELISA. HPV ELISA was performed on serum samples from 50 healthy individuals and 50 patients with OSCC. RESULTS: Using the HPV ELISA, 30% (OR = 2.25, 95% CI = 0.85-5.93) and 18% (OR = 1.61, 95% CI = 0.53-4.92) of patients with oral cancer were found to be HPV 16 E6 and E7 seropositive, respectively. Significant association was found between HPV 16 seropositivity and increased risk of OSCC in men, but not in male subjects. A similar trend was observed in non-betel quid chewers. CONCLUSIONS: Potential associations between HPV 16 E6/E7 seropositivity and oral cancer were revealed in men and non-betel quid chewer subjects, suggesting a possible etiological role of HPV 16 in subgroup of patients with OSCC in Malaysia.
Assuntos
Alphapapillomavirus/isolamento & purificação , Carcinoma de Células Escamosas/virologia , Neoplasias Bucais/virologia , Proteínas Oncogênicas Virais/sangue , Proteínas E7 de Papillomavirus/sangue , Proteínas Repressoras/sangue , Adulto , Estudos de Casos e Controles , Humanos , Pessoa de Meia-Idade , Fatores de RiscoRESUMO
We have used hydroelastic waves to remove small gallstones in hepatic and common bile ducts. These stones were shaken off by the action of progressive and reflected traveling waves. The mechanical properties of the bile ducts were measured and an analysis of the propagation of hydroelastic waves in the liver-bile ducts was made. A practical system was designed, constructed, and clinically tested to remove the gallstones that remained in the hepatic or common bile ducts after surgical operations. The system was found to be effective.