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1.
Appl Opt ; 61(14): 4215-4225, 2022 May 10.
Artigo em Inglês | MEDLINE | ID: mdl-36256099

RESUMO

During 2019, an infrared camera, the compact thermal imager (CTI), recorded 15 million images of the Earth from the International Space Station. CTI is based on strained-layer superlattice (SLS) detector technology. The camera covered the spectral range from 3 to 11 µm in two spectral channels, 3.3-5.4 and 7.8-10.7 µm. Individual image frames were 26×21km2 projected on the ground, with 82 m pixel resolution. A frame time of 2.54 s created continuous image swaths with a 13% along-track image overlap. Upper limits determined on the ground and in flight for the electronic offset, read noise, and dark current demonstrated the stability of the SLS detector and camera over many months. Temperature calibration was established using a combination of preflight and in-flight measurements. A narrowband approximation of temperature as a function of photon counts produced an analytic relationship covering a temperature range of 0°-400°C. Examples of CTI images illustrate temperature retrievals over sea ice, urban and agricultural areas, desert, and wildfires.

2.
J Biol Regul Homeost Agents ; 34(1): 93-100, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32400148

RESUMO

Endometriosis is a gynecological health problem for women of reproductive stage. Kallikrein 4 is a proliferative factor and has important roles in cancer development and progression. To explore the role of Kallikrein 4 in endometriosis, we detected the expression of Kallikrein 4 in ectopic and normal control endometriosis tissues. Moreover, the underlying influence of Kallikrein 4 on migration and invasion of endometrial stromal cells was investigated. Furthermore, the correlations between this gene and E-cadherin and N-cadherin were also evaluated. The results demonstrated that the expression level of Kallikrein 4 in endometrial tissues was significantly increased compared to normal endometrial tissues, and over-expression of Kallikrein 4 up-regulated expression of N-cadherin but down-regulated E-cadherin in endometrial stromal cells. The ability of migration and invasion of endometrial stromal cells in vitro was increased by up-regulating Kallikrein 4 expression, suggesting that Kallikrein 4 might be involved in the development of ovarian endometriosis.


Assuntos
Movimento Celular , Endometriose/fisiopatologia , Transição Epitelial-Mesenquimal , Calicreínas/metabolismo , Células Estromais/citologia , Caderinas/metabolismo , Proliferação de Células , Endométrio/citologia , Endométrio/patologia , Feminino , Humanos
3.
Zhonghua Yi Xue Za Zhi ; 97(43): 3401-3405, 2017 Nov 21.
Artigo em Zh | MEDLINE | ID: mdl-29179281

RESUMO

Objective: To determine the effects of different computed high b-value on diffusion weighted imaging (DWI) scores in Prostate Imaging Reporting and Data System version 2 (PI-RADS v2) of prostate cancer in peripheral zone. Methods: A retrospective study of 104 cases of prostate cancer in peripheral zone was conducted, all of the patients were histopathologically confirmed by transrectal ultrasound guided saturation biopsy or radical prostatectomy in Tongji Hospital of Tongji University from January 2012 to December 2015.All MRI imaging examinations were performed by using a 3.0T Siemens Verio MRI scanner.The imaging protocol consisted of high-resolution axial and sagittal T(2) weighted imaging (T(2)WI), axial acquired diffusion weighted imaging (aDWI) with b=0, 50, 1 000 s/mm(2) and dynamic contrast-enhanced (DCE) scans.Computed diffusion weighted imaging (cDWI) images with b=1 000, 1 400, 2 000 s/mm(2) were processed by Matlab.These three groups of cDWI images were analyzed according to the PI-RADS v2 criteria, and signal intensity of ratio (SIR) of lesions were analyzed by independent t test and one-way ANOVA in each group. Results: The numbers of cases with a DWI score of 2 and 5 were similar among three groups.Nine cases (33.3%) in all 27 cases with a DWI score of 3 on b=1 000 s/mm(2) upgraded to score 4 when b-value rose to 1 400 and 2 000 s/mm(2).The ratios of SIR of lesions in cases upgraded from DWI score 3 to 4 to those unchanged cases on b=1 400 and 2 000 s/mm(2) were 1.86±0.21 to 1.61±0.27 and 2.18±0.26 to 1.75±0.30, respectively (t=2.486, t=3.671, both P<0.05). In these 9 cases who upgraded to DWI score 4, SIRs of the lesion were significantly different between groups when b=1 000, 1 400 and 2 000 s/mm(2) (F=10.907, 33.768, 8.043, all P<0.05), and their SIRs increased with the rising of b-value. Conclusions: The computed high b-value (b≥1 400 s/mm(2)) mainly affects cases with a DWI score of 3, but DWI scores would not change neither in b=1 400 s/mm(2) nor in 2 000 s/mm(2) cases. For DWI score in PI-RADS v2 of prostate cancer in peripheral zone, b=1 400 s/mm(2) is probably of more scoring value than b=1 000 or 2 000 s/mm(2).


Assuntos
Imagem de Difusão por Ressonância Magnética , Neoplasias da Próstata/diagnóstico por imagem , Humanos , Imageamento por Ressonância Magnética , Masculino , Estudos Retrospectivos
4.
Geophys Res Lett ; 43(10): 5450-5459, 2016 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-32753770

RESUMO

Cloud observations from the CloudSat and CALIPSO satellites helped to explain the reduced total cloud cover (Ctot) in the atmospheric regional climate model HIRHAM5 with modified cloud physics. Arctic climate conditions are found to be better reproduced with (1) a more efficient Bergeron-Findeisen process and (2) more generalized subgrid-scale variability of total water content. As a result, the annual cycle of Ctot is improved over sea ice, associated with an almost 14% smaller area average than in the control simulation. The modified cloud scheme reduces the Ctot bias with respect to the satellite observations. Except for autumn, the cloud reduction over sea ice improves low-level temperature profiles compared to drifting station data. The HIRHAM5 sensitivity study highlights the need for improving accuracy of low-level (< 700m) cloud observations, as these clouds exert a strong impact on the near-surface climate.

5.
Andrologia ; 48(4): 406-24, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26276351

RESUMO

Overweight and obese males might exhibit a great risk of infertility. However, according to the current studies, the association between elevated male body mass index (BMI) and the clinical adverse results after assisted reproductive technology (ART) remains controversial. Hence, we conducted a meta-analysis to evaluate the effects of raised male BMI on clinical outcomes following ART. PubMed, EMBASE and three Chinese databases were used to identify relevant studies. The primary outcome was clinical pregnancy rate. Secondary outcomes included live birth rate and sperm parameters. A total of 5262 male participants from 10 cohort studies were subjected to meta-analysis. Results indicated that overweight or obese had no significant impact on clinical pregnancy rate [in vitro fertilisation (IVF): odds ratio (OR), 0.73; 95% confidence interval (CI), 0.39-1.39; intracytoplasmic sperm injection (ICSI): OR, 1.03; 95% CI, 0.92-1.15], live birth rate (IVF: OR, 0.91; 95% CI, 0.78-1.06; ICSI: OR, 1.00; 95% CI, 0.50-1.99) and sperm concentration (SMD, -0.28; 95% CI, -0.65 to 0.08) compared with normal weight following IVF/ICSI treatments. Exclusion of any single study and almost all the sensitivity analyses showed that our results were reliable. At present, the role of male BMI in the process of ART is only partly understood and should be further investigated.


Assuntos
Índice de Massa Corporal , Taxa de Gravidez , Técnicas de Reprodução Assistida/estatística & dados numéricos , Injeções de Esperma Intracitoplásmicas/estatística & dados numéricos , Estudos de Coortes , Feminino , Fertilização in vitro/estatística & dados numéricos , Humanos , Infertilidade Masculina/etiologia , Infertilidade Masculina/terapia , Nascido Vivo/epidemiologia , Masculino , Obesidade/complicações , Sobrepeso/complicações , Gravidez , Reprodutibilidade dos Testes , Contagem de Espermatozoides , Resultado do Tratamento
6.
Genet Mol Res ; 15(2)2016 Apr 04.
Artigo em Inglês | MEDLINE | ID: mdl-27173176

RESUMO

Full-length cDNA of the gene checkpoint homolog 1 (Chk1) was cloned from Daphnia carinata and designated DcarChk1. DcarChk1 cDNA was 1817 bp in length and encoded a 497-amino acid polypeptide. Phylogenetic analyses revealed that DcarChk1 was most closely related to Chk1 of Daphnia pulex, followed by homologous genes of insects. Expression of DcarChk1 was higher in adult Daphnia than in larvae, and significantly higher in males than females, as determined by real-time polymerase chain reaction analysis. Using whole-mount in situ hybridization techniques, DcarChk1 in parthenogenetic females was found to be expressed mainly on the head surface, capillus, and carapace valve edge. In contrast, in sexual females, DcarChk1 was expressed mainly in the joint of the second antenna, and in the thoracic limbs and capillus. These results suggest that DcarChk1 plays a significant role in both the growth and development, as well as in regulating reproductive plasticity, in D. carinata.


Assuntos
Proteínas de Ciclo Celular/genética , Daphnia/genética , Regulação da Expressão Gênica no Desenvolvimento , Proteínas Serina-Treonina Quinases/genética , Animais , Proteínas de Ciclo Celular/química , Proteínas de Ciclo Celular/metabolismo , Clonagem Molecular , Daphnia/crescimento & desenvolvimento , Feminino , Masculino , Especificidade de Órgãos , Proteínas Serina-Treonina Quinases/química , Proteínas Serina-Treonina Quinases/metabolismo
7.
Appl Microbiol Biotechnol ; 99(2): 729-39, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25038929

RESUMO

The Bluetongue virus (BTV) VP7 protein represents an important group-specific antigen that can serve as a basis for diagnostic tests. Here, we report the generation of a novel BTV group-specific monoclonal antibody (Mab; herein named 4H7) that recognizes a conformational epitope in the VP7 protein. We used a phage-displayed peptide screen and site-directed mutagenesis to define the VP7 amino acid residues that most strongly contribute to the conformational epitope recognized by Mab 4H7. Amino acid residues at positions 175, 185, 186, and 278 of the BTV VP7 protein strongly contributed to Mab 4H7 binding. These key amino acid residues are conserved among all BTV serotypes, whereas related Orbiviruses possess at least one amino acid substitution at these positions. We developed a competitive enzyme-linked immunosorbent assay (c-ELISA) using Mab 4H7 and recombinant BTV VP7 protein to detect serum antibodies against this BTV group-specific VP7 epitope. The c-ELISA was used to screen 833 clinical samples collected from animals in three provinces of China. BTV seroprevalence in the three provinces ranged from 25.42 to 47.45 %. This work provides the foundation for a new diagnostic c-ELISA that can be further applied to BTV surveillance activities and informs our understanding of the structure of the BTV VP7 protein.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Vírus Bluetongue/imunologia , Ensaio de Imunoadsorção Enzimática/métodos , Animais , Anticorpos Monoclonais/sangue , Anticorpos Antivirais/sangue , China , Clonagem Molecular , Epitopos/sangue , Epitopos/imunologia , Cabras/virologia , Mutagênese Sítio-Dirigida , Conformação Proteica , Proteínas Recombinantes/sangue , Proteínas Recombinantes/imunologia , Reprodutibilidade dos Testes , Estudos Soroepidemiológicos , Ovinos/virologia , Proteínas do Core Viral/sangue , Proteínas do Core Viral/imunologia
8.
Appl Microbiol Biotechnol ; 98(7): 3033-47, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24337223

RESUMO

VP5, the outer capsid protein of bluetongue virus (BTV), plays an important role in viral penetration and antibody-mediated viral neutralization. Therefore, VP5 represents an important target for development of vaccines and diagnostic tests. In this study, we use bioinformatic tools to predict nine antigenic B cell epitopes in the VP5 protein of a BTV serotype 4 (BTV4) isolate from China. Further, we generate five BTV4 VP5-specific monoclonal antibodies (MAbs) and define their corresponding epitopes using a set of VP5-derived peptides expressed as maltose-binding protein (MBP) fusion proteins. The five identified epitopes map to amino acids 119-134, 257-272, 286-301, 322-337, and 481-496 of the VP5 protein. Importantly, the epitopes identified using VP5-derived peptides do not correlate with our bioinformatic prediction of antibody epitopes. Identification and characterization of BTV4 VP5 protein epitopes may aid the development of diagnostic tools and provide information with which to study the structure of the BTV VP5 protein.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Proteínas do Capsídeo/genética , Proteínas do Capsídeo/imunologia , Mapeamento de Epitopos , Epitopos de Linfócito B/genética , Epitopos de Linfócito B/imunologia , Anticorpos Monoclonais/isolamento & purificação , Anticorpos Antivirais/isolamento & purificação , Vírus Bluetongue/genética , Vírus Bluetongue/imunologia , China , Simulação por Computador
9.
Arch Virol ; 158(5): 1099-104, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23292067

RESUMO

Bluetongue virus (BTV) VP2 is an important antigenic protein that can be used for the differential diagnosis of different BTV serotypes. Here, we generated a serotype-specific monoclonal antibody (mab) against BTV1. A series of peptides synthesized based on the amino acid sequence of BTV1 VP2 were screened to define (115)AQPLKVGL(122) as the minimal linear peptide epitope recognized by mab 4B6. Using an immunofluorescence assay (IFA), we found that mab 4B6 reacted strongly with BTV1, but did not react with other BTV serotypes (BTV2-24). The 4B6 will serve as a novel reagent in the development of diagnostic tests for BTV1 infection.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Antivirais/imunologia , Vírus Bluetongue/imunologia , Proteínas do Capsídeo/imunologia , Epitopos de Linfócito B/imunologia , Vírus Bluetongue/classificação , Mapeamento de Epitopos , Imunofluorescência , Sorotipagem
10.
Mycotoxin Res ; 39(3): 193-200, 2023 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-37237114

RESUMO

Highland barley, also called "qingke" in Tibetan, is mainly cultivated in the Tibetan Plateau of China and has been used as a major staple food for Tibetans. Recently, Fusarium head blight (FHB) of qingke was frequently observed around the Brahmaputra River in Tibet. Considering the importance of qingke for Tibetans, the assessment of Fusarium mycotoxin contamination is essential for food safety. In this study, a total of 150 freshly harvested qingke grain samples were obtained from three regions around the Brahmaputra River in Tibet (China) in 2020. The samples were investigated for the occurrence of 20 Fusarium mycotoxins using high-performance liquid chromatography-tandem mass spectrometry (HPLC‒MS/MS). The most frequently occurring mycotoxin was enniatin B (ENB) (46%), followed by enniatin B1 (ENB1) (14.7%), zearalenone (ZEN) (6.0%), enniatin A1 (ENA1) (3.3%), enniatin A (ENA) (1.3%), beauvericin (BEA) (0.7%), and nivalenol (NIV) (0.7%). Due to the increase in altitude, the cumulative precipitation level and average temperature decreased from the downstream to the upstream of the Brahmaputra River; this directly correlated to the contamination level of ENB in qingke, which gradually decreased from downstream to upstream. In addition, the level of ENB in qingke obtained from qingke-rape rotation was significantly lower than that from qingke-wheat and qingke-qingke rotations (p < 0.05). These results disseminated the occurrence of Fusarium mycotoxins and provided further understanding of the effect of environmental factors and crop rotation on Fusarium mycotoxins.


Assuntos
Fusarium , Hordeum , Micotoxinas , Micotoxinas/análise , Tibet , Fusarium/química , Espectrometria de Massas em Tandem , Contaminação de Alimentos/análise , China , Grão Comestível/química
11.
Eur Rev Med Pharmacol Sci ; 24(19): 9797-9806, 2020 10.
Artigo em Inglês | MEDLINE | ID: mdl-33090382

RESUMO

OBJECTIVE: Some patients with rheumatoid arthritis (RA) will recur despite they have achieved clinical remission after treatment. The subclinical synovitis detected by ultrasonography (US) may be one of the main causes of the RA recurrence. The aim of this study is to establish a nomogram for predicting the outcome of RA patients with disease in clinical remission. PATIENTS AND METHODS: One hundred and sixty-seven RA patients who achieved clinical remission and were willing to receive a 1-year follow-up were included in this study. Their demographic, clinical, and laboratory characteristics were recorded at baseline. 7-joints ultrasound (US7) synovitis score (simplified from US7 score) were evaluated at baseline and at the end of follow-up (or when RA recurrence confirmed). All patients were divided into recurrence group and non-recurrence group after the follow-up. Multivariable regression was applied to link the predictors that were significant at p < 0.05 in the univariate analysis and the recurrence of RA patients in clinical remission, which was served as the basis of the nomogram. RESULTS: Fifty-one RA patients were included in the recurrence group and 116 patients in the non-recurrence group. All US7 synovitis scores in this study showed excellent reproducibility. Multivariable analysis revealed that high-titer positive anti-cyclic citrullinated peptide (anti-CCP), simplified clinical disease activity index (SDAI), baseline grayscale ultrasound (GSUS) score, and baseline power Doppler ultrasound (PDUS) score were the independent predictors for RA recurrence within 1 year. A nomogram incorporating the independent predictors was constructed to predict the risk of RA recurrence. The nomogram showed good discrimination (C-index=0.826) and good calibration. CONCLUSIONS: The nomogram incorporating anti-CCP, SDAI, and subclinical synovitis helps to achieve complete remission and reduces the risk of short-term recurrence of RA patients.


Assuntos
Artrite Reumatoide/diagnóstico por imagem , Antirreumáticos/uso terapêutico , Artrite Reumatoide/tratamento farmacológico , Estudos de Coortes , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Recidiva , Indução de Remissão , Reprodutibilidade dos Testes
12.
Acta Neurochir Suppl ; 101: 153-5, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-18642651

RESUMO

BACKGROUND: Posterolateral fusion with cages and posterior instrumentation is an accepted method in the treatment of lumbar instability associated with spinal stenosis or scoliotic deformity, but with modest results. We propose hereby an alternative, simple method to treat kyphosis due to the vertebral fracture which has brought about comparable outcomes. METHODS: Three patients with documented adjacent segment compression fractures were treated. Vertebroplasty was performed with polymethylmethacrylate (PMMA), either using the transpedicular route at the adjacent level or via the route of the previous transpedicular screw at the top level of the long-segment fixation construct. Outcomes were measured by the visual analogue scale of pain and the kyphotic angle of the adjacent segment. RESULTS: The maximal kyphotic angle was 30.6 degrees preoperatively and the reduction rate averaged 69.6%. The pain scale improved from the mean of 9.3 to 1.7. No further progression of compression was noted in the follow-up of more than 6 months after the vertebroplasty in these cases. CONCLUSION: Vertebroplasty at either the adjacent level or the top level of the previous internal fixation construct may be a feasible alternative to treat the adjacent level fracture after long segment internal fixation of the spine.


Assuntos
Cimentos Ósseos/uso terapêutico , Procedimentos Ortopédicos/métodos , Polimetil Metacrilato/uso terapêutico , Fraturas da Coluna Vertebral/etiologia , Fraturas da Coluna Vertebral/terapia , Fusão Vertebral/efeitos adversos , Idoso , Feminino , Humanos , Vértebras Lombares , Masculino , Pessoa de Meia-Idade , Doenças da Medula Espinal/cirurgia , Fusão Vertebral/métodos
13.
Prostate Cancer Prostatic Dis ; 10(1): 104-8, 2007.
Artigo em Inglês | MEDLINE | ID: mdl-17043678

RESUMO

The relationship between chronic prostatitis and fertility has been disputed for many years. Several groups have shown infection and autoimmune response against prostate antigens could have a deleterious effect on semen quality and fertility. This study was conducted to test the hypothesis that Omi/HtrA2-induced apoptosis in chronic prostatitis could be a mechanism underlying the observed clinical benefit. The Omi/HtrA2 serine protease is a nuclear-encoded mitochondrial protein, which can be released from mitochondria into the cytosol after apoptosis stimuli, inducing apoptosis in caspase-dependent and independent manners. Forty-one patients diagnosed as suffering from chronic prostatitis were included. Healthy normal individuals were included as controls. Human spermatozoa in the semen were purified by Percoll-gradient technique to separate the seminal plasma and other round cells. Measurements for sperm concentration, motility, morphology, proinflammatory cytokines, Omi/HtrA2 mRNA and protein levels in spermatozoa of chronic protatitis patients, were performed accordingly. Significantly increased levels of proinflammatory cytokines were detected in seminal plasma from these prostatitis patients. Omi/HtrA2 mRNA and protein levels were significantly higher in prostatitis men than in normal men. This study shows that chronic prostatitis patients present important alterations in their semen quality parameters, Omi/HtrA2 mRNA and protein levels of spermatozoa. We speculate that the inflammatory process involved may affect male fertility by release of proapoptotic protein Omi/HtrA2.


Assuntos
Proteínas Mitocondriais/metabolismo , Prostatite/fisiopatologia , Sêmen/fisiologia , Serina Endopeptidases/metabolismo , Espermatozoides/metabolismo , Adolescente , Adulto , Proteínas Reguladoras de Apoptose/metabolismo , Serina Peptidase 2 de Requerimento de Alta Temperatura A , Humanos , Infertilidade Masculina/etiologia , Mediadores da Inflamação/metabolismo , Masculino , Pessoa de Meia-Idade
14.
J Virol Methods ; 234: 28-33, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27063641

RESUMO

Bluetongue (BT), which is caused by the BT virus (BTV), is an important disease in ruminants that leads to significant economic losses in the husbandry industry. To detect BTV-specific antibodies in serum, a protein chip detection method based on a novel solid supporting material known as polymer-coated initiator-integrated poly (dimethyl siloxane) (iPDMS) was developed. With a threshold of 25% (signal-to-noise percentage), the sensitivity and specificity of the protein chip were 98.6% and 94.8%, respectively. Furthermore, spot serum samples obtained from six provinces of China were tested with the protein chip and a commercially available BTV enzyme-linked immunosorbent assay (ELISA) kit (IDEXX). Of 615 samples, BTV-specific antibodies were detected in 200 (32.52%) by the protein chip and in 176 (28.62%) by the IDEXX BTV ELISA kit. Comparison of the protein chip with the commercial IDEXX BTV ELISA kit yielded the following spot serum detection results: a total coincidence, a negative coincidence and a positive coincidence of 95.12%, 99.28% and 86.5%, respectively. With the protein chip, the BTV-specific serum antibody was detected in samples from all six provinces, and the positive rates ranged from 4.12 to 74.4%. These results indicate that this protein chip detection method based on iPDMS is useful for the serological diagnosis of BTV infection and for epidemiological investigation.


Assuntos
Anticorpos Antivirais/sangue , Vírus Bluetongue/imunologia , Bluetongue/diagnóstico , Análise Serial de Proteínas/métodos , Animais , Bluetongue/sangue , Bluetongue/epidemiologia , Bluetongue/virologia , Vírus Bluetongue/genética , Bovinos/virologia , Doenças dos Bovinos/diagnóstico , Doenças dos Bovinos/virologia , China/epidemiologia , Ensaio de Imunoadsorção Enzimática/métodos , Análise Serial de Proteínas/instrumentação , Sensibilidade e Especificidade , Ovinos/virologia
15.
Transbound Emerg Dis ; 63(6): 585-589, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27597166

RESUMO

One hundred and twenty-six blood samples were collected from healthy sheep and goats in Xinjiang, China, during July 2014. Seventy-three samples (57.93%) were bluetongue virus (BTV) serology-positive, and 39 samples (30.95%) were BTV NS1 gene-positive. BTV strain XJ1407 was isolated from the blood of BTV NS1 gene-positive animals and sequenced. Analysis of its genome sequence suggests that XJ1407 is a novel BTV serotype.


Assuntos
Vírus Bluetongue/genética , Vírus Bluetongue/isolamento & purificação , Doenças Transmissíveis Emergentes/veterinária , Sorogrupo , Animais , Sequência de Bases , Bluetongue/virologia , China , Cabras , Ovinos
16.
J Am Coll Cardiol ; 19(2): 355-64, 1992 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-1732365

RESUMO

Electrophysiologic studies with recordings of sinus node electrograms were performed in 38 patients with severe symptomatic sick sinus syndrome. Thirty-two of the 38 patients had episodic tachyarrhythmias and 17 presented with syncope. The clinically documented sinus or atrial pause was 5.6 +/- 2.8 s (mean +/- SD). Patients were divided into three groups according to electrophysiologic findings. Group I consisted of nine patients with complete sinoatrial block. Sinus node electrograms were recorded during the episodes of long pauses. Seven patients had unidirectional exit block, with the atrial impulse being capable of retrograde penetration to the sinus node causing suppression of sinus automaticity; two had bidirectional sinoatrial block. Group II consisted of 22 patients with either 1:1 sinoatrial conduction (group IIa = 13 patients) or second degree sinoatrial exit block (group IIb = 9 patients) during spontaneous sinus rhythm. Sinoatrial exit block, ranging from 1 to greater than 14 sinus beats, was observed during postpacing pauses that ranged from 1,650 to 37,000 ms (mean 7,286 +/- 6,989). The maximal sinus node recovery time ranged from 770 to 5,580 ms (mean 3,004 +/- 1,686) and was normal in 5 patients and prolonged in 17. Group III consisted of seven patients with no recordable sinus node electrogram, reflecting either a technical failure or a quiescence of sinus activity. The sinus node recovery time in these seven patients ranged from 1,190 to 4,260 ms (mean 2,949 +/- 1,121). Thus, abnormalities in both sinus node automaticity and sinoatrial conduction are responsible for the long sinus or atrial pauses in the sick sinus syndrome. However, complete sinoatrial exit block can occur and cause severe bradycardia with escape rhythm; repetitive sinoatrial exit block plays a major role in producing posttachycardia pauses.


Assuntos
Síndrome do Nó Sinusal/fisiopatologia , Nó Sinoatrial/fisiopatologia , Estimulação Cardíaca Artificial , Eletrocardiografia , Eletrofisiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Síndrome do Nó Sinusal/diagnóstico , Bloqueio Sinoatrial/fisiopatologia
17.
Am J Cardiol ; 65(22): 1438-42, 1990 Jun 15.
Artigo em Inglês | MEDLINE | ID: mdl-2353648

RESUMO

The effects of isoproterenol on accessory pathway conduction were evaluated in 24 patients with intermittent and 60 patients with concealed pre-excitation, using atrial and ventricular incremental and extrastimulus testing techniques. The atrial paced cycle length that induced block in the accessory pathway could be compared in 11 of the 24 patients with intermittent preexcitation before and after isoproterenol; it decreased from 622 +/- 212 ms to 408 +/- 128 ms (mean +/- standard deviation) after isoproterenol (p less than 0.01). The anterograde effective refractory period of the accessory pathway could be compared in 5 patients before and after isoproterenol; it decreased from 460 +/- 131 to 310 +/- 48 ms after isoproterenol (p less than 0.01). None of the 60 patients with concealed preexcitation showed ventricular preexcitation with isoproterenol infusion. Eighty-one of the 84 patients had clinically documented supraventricular tachycardia, suggesting the accessory pathway was capable of retrograde conduction. Retrograde study was performed in all 84 patients; 83 had retrograde conduction and the other had no retrograde conduction before and after isoproterenol. The ventricular paced cycle length that induced block in the accessory pathway could be compared in 38 patients before and after isoproterenol; it decreased from 342 +/- 71 to 296 +/- 39 ms after isoproterenol (p less than 0.001). The retrograde effective refractory period of the accessory pathway could be compared in 56 patients; it decreased from 293 +/- 76 to 238 +/- 36 ms after isoproterenol (p less than 0.001). In conclusion, isoproterenol facilitates anterograde and retrograde accessory pathway conduction, but the facilitation of anterograde conduction occurs only in those capable of spontaneous conduction.


Assuntos
Sistema de Condução Cardíaco/fisiopatologia , Isoproterenol/farmacologia , Síndrome de Wolff-Parkinson-White/fisiopatologia , Adolescente , Adulto , Idoso , Estimulação Cardíaca Artificial , Eletrocardiografia/efeitos dos fármacos , Feminino , Sistema de Condução Cardíaco/efeitos dos fármacos , Humanos , Isoproterenol/administração & dosagem , Masculino , Pessoa de Meia-Idade
18.
Vet Immunol Immunopathol ; 55(4): 283-90, 1997 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-9151400

RESUMO

Immunohistologic studies were performed to identify the phenotype and distribution of neoplastic lymphocytes in the spleens of BLV-negative animals examined by PCR and diagnosed as having sporadic bovine leukosis. Tumor cells from three cases of sporadic bovine leukosis were identified as of B-cell lineage. Tumor cells from three additional cattle were identified as CD3+ CD4- CD8+, CD3+ CD4- CD8-, and CD3+ CD4- WC1+, respectively. The last case was diagnosed as a gamma/delta T-cell lymphoma. Differences in morphology proliferative characteristics were recognized between B- and T-cell type lymphomas. The tumor cells in B-cell type lymphoma were characterized as follows: medium or large in size, round or polymorphic nucleus with rough chromatin with some tumor cells containing a convoluted nucleus. These tumor cells of B-cell type lymphoma were present in the red pulp and periarteriolar lymphoid sheath. Tumor cells of the T-cell type lymphoma were uniformly smaller than B-cell type and present around arteries or replaced red pulp of the spleen.


Assuntos
Doenças dos Bovinos/patologia , Linfonodos/patologia , Linfoma de Células B/patologia , Linfoma de Células B/veterinária , Linfoma de Células T/patologia , Linfoma de Células T/veterinária , Baço/patologia , Animais , Bovinos , Feminino , Imuno-Histoquímica , Imunofenotipagem , Linfonodos/imunologia , Linfoma de Células B/classificação , Linfoma de Células T/classificação , Masculino , Baço/imunologia
19.
Pharmacol Biochem Behav ; 40(1): 97-101, 1991 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-1780352

RESUMO

The effect of single and repeated marijuana smoke exposure on fetal EEG was investigated in the chronic fetal lamb model using power spectral analysis. Maternal inhalation of marijuana smoke (n = 9) resulted in a significant reduction in total power and power distribution in the delta (1-4 Hz) band, and an increase in power distribution in the faster frequencies in the first h after smoke inhalation. These EEG changes were not observed following maternal inhalation of placebo smoke (n = 5), nor in animals with 3-5 prior exposures to marijuana smoke (n = 5). These results suggest that the effects of marijuana smoke exposure on fetal EEG is short-lived and tolerance develops rapidly with repeated exposure.


Assuntos
Eletroencefalografia/efeitos dos fármacos , Feto/fisiologia , Fumar Maconha/fisiopatologia , Animais , Feminino , Gravidez , Ovinos
20.
Pharmacol Biochem Behav ; 49(4): 795-800, 1994 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-7886089

RESUMO

Opiates are known to exert biphasic effects on level of arousal, with excitation at low doses and depression at higher doses. It has been suggested that this dual excitatory and depressant actions of opiates may be mediated by different receptor subtypes. We have previously shown that activation of mu 1-opioid receptors evoked EEG activation in the fetal lamb. The purpose of the present study was to quantitate the effects of DPDPE, a highly selective delta-opioid agonist, on fetal EEG. When infused ICV (4.6-154 nmol/h), DPDPE elicited dose-dependent activation of fetal EEG, with a reduction in power distribution in the delta (1-4 Hz) band, and an increase in the beta (15-32 Hz) band. This activation was reflected by an increase in the spectral edge frequency. This EEG activation was greatly attenuated at DPDPE doses greater than 154 nmol/h, resulting in a U-shaped dose-response curve. The EEG activation was completely blocked by naloxone or naltrindole (delta antagonist), but not by naloxonazine (mu 1 antagonist). These results indicate that the activation of delta-opioid receptors will evoke EEG activation in the fetal lamb.


Assuntos
Analgésicos/farmacologia , Eletroencefalografia/efeitos dos fármacos , Encefalinas/farmacologia , Feto/fisiologia , Receptores Opioides delta/agonistas , Analgésicos/administração & dosagem , Analgésicos/antagonistas & inibidores , Animais , Relação Dose-Resposta a Droga , D-Penicilina (2,5)-Encefalina , Encefalinas/administração & dosagem , Encefalinas/antagonistas & inibidores , Feminino , Injeções Intraventriculares , Naltrexona/análogos & derivados , Naltrexona/farmacologia , Antagonistas de Entorpecentes/farmacologia , Gravidez , Receptores Opioides delta/antagonistas & inibidores , Ovinos
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