RESUMO
To prepare tanshinone â ¡A loaded nanostructured lipid carrier (Tan â ¡A-NLC), and study its in vitro transdermal permeation characteristics. The Tan â ¡A-NLC was prepared by high pressure homogenization technology and optimized by Box-Behnken design-response surface method, and it was characterized in terms of morphology, particle size, zeta potention, et al. The transdermal permeation of Tan â ¡A-NLC was evaluated by using Franz diffusion cells. The results showed that, the optimal formulation was as follows: drug/lipid materials ratio 88, GMS/MCT ratio 2, emulsifier concentration 1%, average particle size (182±14) nm, polydispersity index PDI (0.190 6±0.024 5), zeta potential (ï¼27.8± 5.4) mV, encapsulation efficiency EE (86.44%±9.26%) and drug loading DL (0.98%±0.18%), respectively. The in vitro transdermal permeation results showed that as compared with Tan â ¡A solution, Tan â ¡A-NLC had lower transdermal permeation amount after applying drug for 24 h, but its retention in the epidermis was 3.18 times that of solution. These results indicated that the prepared Tan â ¡A-NLC could effectively increase the regention of Tan â ¡A in the epidermis, and had a broad application prospect.