[Research of the relation between the type of asthenia of the spleen and kidney and platelet associated antibodies and T-lymphocyte subsets in idiopathic thrombocytopenic purpura].

Idiopathic thrombocytopenic purpura (ITP) is a kind of disease associated with immunity. At present a great quantity of study on ITP has been made on humoral and cellular immunity. But there are few reports about the relationship between the types based on the differential diagnosis of TCM and immune rationale of ITP. In order to deeply explore the relationship between the types based on differential diagnosis of TCM and immune rationale of ITP., the authors measured PAIg and T lymphocyte subsets of 34 ITP patients of asthenia of both Spleen and Kidney. The value of PAIgG increased in both types of Spleen failing to control blood (SFCB) and deficiency of Spleen-yin and Kidney -yin (DSYKY), and the value of PAIgG of the type of DSYKY was significantly higher than that of SFCB (P less than 0.01). OKT3, OKT4/OKT8 of the type of SFCB remarkably decreased (P less than 0.05), OKT4, OKT4/OKT8 of DSYKY also remarkably decreased (P less than 0.001), while OKT8 significantly increased (P less than 0.001). The above results suggested that the type of DSYKY has more serious immune dysfunction than the type of SFCB, and the types of SFCB and DSYKY has close relationship with PAIg T lymphocyte subsets.

[In vitro effect of shen-xue-ling on megakaryocytopoiesis in patients with idiopathic thrombocytopenic purpura].

This paper, used an improved methylcellulose-agar double layer method in vitro to culture megakaryocyte progenitors (CFU-meg) of 15 normal bone marrow donors and 20 idiopathic thrombocytopenic purpura (ITP) patients bone marrow cells in order to observe the action of Shen-Xue-Ling (SXL). The results showed that the colony efficiency of CFU-meg of ITP group (13.75 +/- 6.93/10(5) MNC) was obviously lower than that of the control group (21.22 +/- 9.06/10(3) MNC, P less than 0.01). The serum of the patients whose PAIgG was markedly higher than normal value inhibited growth of CFU.meg derived from normal bone marrow cells (P less than 0.01). SXL could increase the colony efficiency of CFU-meg for ITP patients at several doses. The average colonies were 15.77 +/- 6.31/10(5) MNC (at 100 mg/ml, P greater than 0.05), 18.72 +/- 5.54/10(5) MNC (at 200 mg/ml, P less than 0.05), 21.60 +/- 7.35/10(5) MNC (at 300 mg/ml, P less than 0.01) and 21.84 +/- 7.15/10(5) MNC (at 500 mg/ml, P less than 0.01) as compared with the group of ITP patients. Incubated with patient's serum (1%) and SXL (at 300 mg/ml), the number of CFU-meg extracted from normal bone marrow cells was significantly higher than that from patients (P less than 0.01) and was close to normal group (P greater than 0.05). The results suggested that SXL might inhibit the antiplatelet antibodies, facilitate reproduction, division and maturity of CFU-meg.