Stigma receptors control intraspecies and interspecies barriers in Brassicaceae.

Flowering plants have evolved numerous intraspecific and interspecific prezygotic reproductive barriers to prevent production of unfavourable offspring1. Within a species, self-incompatibility (SI) is a widely utilized mechanism that rejects self-pollen2,3 to avoid inbreeding depression. Interspecific barriers restrain breeding between species and often follow the SI × self-compatible (SC) rule, that is, interspecific pollen is unilaterally incompatible (UI) on SI pistils but unilaterally compatible (UC) on SC pistils1,4-6. The molecular mechanisms underlying SI, UI, SC and UC and their interconnections in the Brassicaceae remain unclear. Here we demonstrate that the SI pollen determinant S-locus cysteine-rich protein/S-locus protein 11 (SCR/SP11)2,3 or a signal from UI pollen binds to the SI female determinant S-locus receptor kinase (SRK)2,3, recruits FERONIA (FER)7-9 and activates FER-mediated reactive oxygen species production in SI stigmas10,11 to reject incompatible pollen. For compatible responses, diverged pollen coat protein B-class12-14 from SC and UC pollen differentially trigger nitric oxide, nitrosate FER to suppress reactive oxygen species in SC stigmas to facilitate pollen growth in an intraspecies-preferential manner, maintaining species integrity. Our results show that SRK and FER integrate mechanisms underlying intraspecific and interspecific barriers and offer paths to achieve distant breeding in Brassicaceae crops.

Nasopharyngeal carcinoma: current views on the tumor microenvironment's impact on drug resistance and clinical outcomes.

The incidence of nasopharyngeal carcinoma (NPC) exhibits significant variations across different ethnic groups and geographical regions, with Southeast Asia and North Africa being endemic areas. Of note, Epstein-Barr virus (EBV) infection is closely associated with almost all of the undifferentiated NPC cases. Over the past three decades, radiation therapy and chemotherapy have formed the cornerstone of NPC treatment. However, recent advancements in immunotherapy have introduced a range of promising approaches for managing NPC. In light of these developments, it has become evident that a deeper understanding of the tumor microenvironment (TME) is crucial. The TME serves a dual function, acting as a promoter of tumorigenesis while also orchestrating immunosuppression, thereby facilitating cancer progression and enabling immune evasion. Consequently, a comprehensive comprehension of the TME and its intricate involvement in the initiation, progression, and metastasis of NPC is imperative for the development of effective anticancer drugs. Moreover, given the complexity of TME and the inter-patient heterogeneity, personalized treatment should be designed to maximize therapeutic efficacy and circumvent drug resistance. This review aims to provide an in-depth exploration of the TME within the context of EBV-induced NPC, with a particular emphasis on its pivotal role in regulating intercellular communication and shaping treatment responses. Additionally, the review offers a concise summary of drug resistance mechanisms and potential strategies for their reversal, specifically in relation to chemoradiation therapy, targeted therapy, and immunotherapy. Furthermore, recent advances in clinical trials pertaining to NPC are also discussed.

Cardiac ectopic lymphoid follicle formation in viral myocarditis involving the regulation of podoplanin in Th17 cell differentiation.

Autoimmunity contributes to the pathogenesis of viral myocarditis (VMC), which is characterized by the production of anti-heart autoantibodies (AHA) from lymphoid follicles. Recently, the formation of ectopic lymphoid follicles (ELFs) was reported in heart grafts. However, the existence and role of ELFs in myocardial tissues of VMC remain unclear. This study aimed to explore whether and how cardiac ELFs with germinal centers (GCs) could be generated during the development of VMC. We identified the existence of ELFs and explored the underlying mechanism. In a BALB/c mouse model of VMC, the dynamic myocardial infiltrations of lymphocytic aggregates and expressions of associated lymphorganogenic factors were investigated, accompanied by the detection of the production and location of myocardial AHA. The data indicated ELFs formation in myocardial tissues of VMC, and the number of ELFs was in accordance with the severity of VMC. Moreover, the functional ELFs with GCs were capable of facilitating the production of local AHA. Blocking IL-17 or podoplanin (PDPN) could inhibit cardiac ELFs generation, perhaps due to the negative regulation of PDPN neutralization in Th17 cell proliferation and differentiation. The presence of cardiac ELFs and AHA might offer new opportunities for stratification and early identification of VMC patients.

Release characteristics of mercury in chemical looping combustion of bituminous coal.

This study evaluated the release characteristics of mercury from bituminous coal in chemical looping combustion (CLC) using Australian iron ore as the oxygen carrier in a fixed bed reactor. The effects of several parameters, such as temperature in the fuel reactor (FR) and air reactor (AR), gasification medium in the FR, and reaction atmosphere in the AR, on mercury release characteristics, were investigated. The mercury speciation and release amount in the FR and AR under different conditions were further explored. The results indicate that most of the mercury in coal was released in the FR, while the rest of it was released in the AR. Hg0 was found to be the major species in the released mercury. The results also indicate that a higher temperature in the FR led to an increase in the total mercury release amount and a decrease in Hg0 proportion. However, a higher temperature in the AR resulted in a decrease in the total mercury release amount and Hg0 proportion. The increase in the H2O/CO2 ratio of gasification mediums in the FR was beneficial for the increase in the total mercury release amount and Hg0 proportion. A higher O2 concentration in reaction atmosphere in AR had a negligible effect on the total mercury release amount, but a positive effect on Hg0 oxidization.

[Effect of Bushen Tiaojing Recipe and Xiaoyao Pill on adenohypophysis and ovary in androgen-induced sterile rats: a comparative study].

OBJECTIVE: To observe the effect of Bushen Tiaojing Recipe (BTR) and Xiaoyao Pill (XYP) on the morphology and sex hormones secretion of adenohypophysis and ovaries in androgen-induced sterile rats (ASR). METHODS: Fifty 9-day old SD female rats randomly recruited from total 60 rats were subcutaneously injected with testosterone propionate to establish the ASR model. And the rest 10 rats were recruited as the normal group. Thirty successfully modeled rats were recruited and randomly divided into the model group, the BTR group (administered with BTR suspension), and the XYP group (administered with XYP suspension), 10 in each group. Five weeks later, rats were decapitated in the proestrus. Serum levels of estradiol (E2), progesterone (P), testosterone (T), follicle-stimulating hormone (FSH), and luteinizing hormone (LH) were detected by radioimmunoassay. The morphologies of adenohypophysis and ovary were observed after HE staining. RESULTS: Compared with the normal group, serum E2 and T levels increased, while FSH and LH levels decreased in the model group (all P < 0.01). The morphology of adenohypophysis and ovary was abnormal in the model group. Compared with the model group, serum E2 and T levels decreased, while FSH and LH levels increased in the BTR group and the XYP group (P < 0.05, P < 0.01). Besides, E2 and T levels in the BTR group and FSH levels in the XYP group restored to normal (all P > 0.05). The damaged structure of adenohypophysis and ovary got restored to different degrees. CONCLUSION: BTR and XYP both could improve ovulation failure.

[Effect of Bushen Tiaojing Recipe containing serum on FSH/cAMP-PKA pathway in in vitro cultured human ovarian granular cells].

OBJECTIVE: To explore the potential molecular mechanisms for Bushen Tiaojing Recipe (BTR) improving the endocrine function of ovarian granular cells by observing the effect of BTR containing serum on follicle stimulating hormone/cyclic adenosine monophosphate-protein kinase A (FSH/ cAMP-PKA) pathway in in vitro cultured human ovarian granular cells. METHODS: The primary ovarian granular cells collected from in vitro fertilization-embryo transfer patients were cultured for 24 h. The human and rat serum containing different concentrations of BTR (low, medium, high dose), and their normal serums were co-incubated with ovarian granular cells for 48 h respectively, and then they were divided into the low, medium, high dose BTR groups and the control group. The levels of estradiol (E2), progesterone (P), and cyclic adenosine monophosphate (cAMP) in the culture medium were measured by radioimmunoassay. The protein expression of FSHR in ovarian granular cells was detected by Western Blot. The mRNA expression of follicle stimulating hormone receptor (FSHR) and P450 aromatase (P450arom) in ovarian granular cells were detected by Real-time PCR. RESULTS: In human BTR containing serum groups: Compared with control group, the levels of E2 and cAMP in the culture medium were higher (both P < 0.05) in the medium and high dose BTR groups; the levels of P in the culture medium decreased in the medium dose BTR group (P < 0.01). The protein and mRNA expression of FSHR in ovarian granular cells increased (all P < 0.01), the mRNA expressions of P450arom in ovarian granular cells were higher (P < 0.05, P< 0.01) in the medium and high dose BTR groups. In rat BTR containing serum groups: Compared with the control group, the levels of E2 in the culture medium were higher (all P < 0.01), cAMP in the culture medium were higher (P < 0.05, P < 0.01) in the medium and high dose BTR group; the levels of P in the culture medium decreased in the medium dose BTR group (P < 0.01). The protein and mRNA expression of FSHR in ovarian granular cells were higher (all P < 0.01), the mRNA expression of P450arom in ovarian granular cells increased in the medium and high dose BTR groups (P < 0.05, P < 0.01). CONCLUSION: BTR could possibly improve the endocrine function of ovarian granular cells by regulating main effector molecules FSHR, cAMP, P450arom, and E2 in FSH/cAMP-PKA pathway of ovarian granular cells.

Differential Analysis of Various Moisture Phases on N2 Displacement of CH4 in Coal.

The aim of the study was to elucidate the impact of different moisture phases during gas injection on coalbed methane displacement. The coal samples were treated with two methods: water vapor adsorption and liquid water stirring. The differences in the coal samples treated with various moisture phases during gas injection for coalbed methane displacement were investigated by using the isothermal adsorption curves of CH4, N2, and H2O in coal and N2 displacement of CH4 in coal. The results indicate that variations in the gas adsorption capacity of coal are treated with different moisture phases. The gas adsorption capacities and displacement capacities of the coal samples treated with the water vapor adsorption methods are better than those treated with the stirring methods. In the isothermal adsorption experiment, for the coal samples treated with different moisture phases, at a moisture content of 2.75%, the saturated adsorption capacities of CH4/N2 are 0.204/0.189 (cm3/g), and at a moisture content of 5.63%, the saturated adsorption capacities of CH4/N2 are 0.151/0.139 (cm3/g). In addition, in the displacement experiment, for the coal samples treated with different moisture phases, at a moisture content of 2.75%, the difference in the total gas adsorption capacities is 0.62 cm3/g and the difference in the CH4 adsorption capacities is 0.473 cm3/g, and at a moisture content of 5.63%, the difference in the total gas adsorption capacities is 0.3 cm3/g and the difference in CH4 adsorption capacities is 0.22 cm3/g. For the coal samples treated with various moisture phases, the differences in the CH4/N2 adsorption and displacement capacities are greater at a moisture content of 2.75% than at 5.63%. Notably, the moisture phase has only a marginal influence on the CH4 desorption capacity and desorption rate. The study is important to understand the interactions between coal and moisture.

Sacubitril/Valsartan inhibits M1 type macrophages polarization in acute myocarditis by targeting C-type natriuretic peptide.

Studies have shown that Sacubitril/valsartan (Sac/Val) can reduce myocardial inflammation in myocarditis mice, in addition to its the recommended treatment of heart failure. However, the underlying mechanisms of Sac/Val in myocarditis remain unclear. C-type natriuretic peptide (CNP), one of the targeting natriuretic peptides of Sac/Val, was recently reported to exert cardio-protective and anti-inflammatory effects in cardiovascular systems. Here, we focused on circulating levels of CNP in patients with acute myocarditis (AMC) and whether Sac/Val modulates inflammation by targeting CNP in experimental autoimmune myocarditis (EAM) mice as well as LPS-induced RAW 264.7 cells and bone marrow derived macrophages (BMDMs) models. Circulating CNP levels were higher in AMC patients compared to healthy controls, and these levels positively correlated with the elevated inflammatory cytokines IL-6 and monocyte count. In EAM mice, Sac/Val alleviated myocardial inflammation while augmenting circulating CNP levels rather than BNP and ANP, accompanied by reduction in intracardial M1 macrophage infiltration and expression of inflammatory cytokines IL-1ß, TNF-α, and IL-6. Furthermore, Sac/Val inhibited CNP degradation and directly blunted M1 macrophage polarization in LPS-induced RAW 264.7 cells and BMDMs. Mechanistically, the effects might be mediated by the NPR-C/cAMP/JNK/c-Jun signaling pathway apart from NPR-B/cGMP/NF-κB pathway. In conclusion, Sac/Val exerts a protective effect in myocarditis by increasing CNP concentration and inhibiting M1 macrophages polarization.

Cancer-associated fibroblasts secrete FGF5 to inhibit ferroptosis to decrease cisplatin sensitivity in nasopharyngeal carcinoma through binding to FGFR2.

Cisplatin (DDP)-based chemoradiotherapy is one of the standard treatments for nasopharyngeal carcinoma (NPC). However, the sensitivity and side effects of DDP to patients remain major obstacles for NPC treatment. This research aimed to study DDP sensitivity regulated by cancer-associated fibroblasts (CAFs) through modulating ferroptosis. We demonstrated that DDP triggered ferroptosis in NPC cells, and it inhibited tumor growth via inducing ferroptosis in xenograft model. CAFs secreted high level of FGF5, thus inhibiting DDP-induced ferroptosis in NPC cells. Mechanistically, FGF5 secreted by CAFs directly bound to FGFR2 in NPC cells, leading to the activation of Keap1/Nrf2/HO-1 signaling. Rescued experiments indicated that FGFR2 overexpression inhibited DDP-induced ferroptosis, and CAFs protected against DDP-induced ferroptosis via FGF5/FGFR2 axis in NPC cells. In vivo data further showed the protective effects of FGF5 on DDP-triggered ferroptosis in NPC xenograft model. In conclusion, CAFs inhibited ferroptosis to decrease DDP sensitivity in NPC through secreting FGF5 and activating downstream FGFR2/Nrf2 signaling. The therapeutic strategy targeting FGF5/FGFR2 axis from CAFs might augment DDP sensitivity, thus decreasing the side effects of DDP in NPC treatment.

Insights into the enhanced mechanism of selenium-doped iron nitride carbon catalysts for elemental mercury removal in flue gas.

This study developed a novel selenium-doped metal nitride carbon, Fe-NC-Se, via pyrolysis and impregnated hydrothermal methods for elemental mercury removal from coal-fired flue gas. The Fe-NC-Se demonstrated a remarkable mercury removal performance, achieving an average efficiency of 96.98% within 60 min at an optimal Se/Fe ratio of 2:1 and temperature of 110 °C, which was 2.5 times higher than that of the pristine Fe-NC (iron nitride carbon). Notably, Fe-NC-Se maintained an 84% efficiency in a high SO2 environment (1600 ppm), indicating strong resistance to SO2 poisoning. Long-term testing over 24 h showed a consistent removal efficiency of 84.75%, suggesting potential for recyclability. Advanced characterization techniques, including TEM (transmission electron microscopy) and XPS (X-ray photoelectron spectrometer), along with Density Functional Theory calculations, were employed to explore the removal mechanism. Results indicated that selenium doping enhanced surface charge transfer and the reactivity of surface atoms, facilitating mercury oxidation and sequestration. The oxidized Hg2+ was anchored by Se and partially stabilized by C, N, and Fe atoms, enhancing the catalyst's effectiveness. This work not only advances the design of mercury abatement catalysts but also supports the industrial applicability of Fe-NC-Se in flue gas treatment.

Removal of Hg2+ in wastewater by grafting nitrogen/sulfur-containing molecule onto Uio-66-NH2: from synthesis to adsorption studies.

The remediation of heavy metal deserves to be on the agenda, with the adsorbent design bearing the brunt of it. In this study, the molecule (4, 6-diamino-2-mercaptopyrimidine, DMP) containing thiol (-SH) and amino (-NH2) functional groups was grafted onto Uio-66-NH2, and a composite metal-organic framework nanomaterial (Zr(NH2)-DMP) was synthesized via a facile post-modification scheme. The morphological characteristics and structural features of the modified adsorbent were characterized by XRD, FT-IR, FE-SEM, EDS, BET, and XPS. The characterization results verified that the post-modification scheme was successfully achieved. The adsorption experiments were carried out to investigate the removal performance of the Zr(NH2)-DMP towards Hg2+ under different influencing parameters. The maximum adsorption capacity of 389.4 mg/g was obtained, and the adsorption equilibrium was achieved within 30 min at pH 6 at room temperature. Adsorption thermodynamic study indicated that the adsorption process was exothermic and spontaneous. The Zr(NH2)-DMP exhibited excellent selectivity for Hg2+, and also has the potential to remove Cu2+, Fe2+, and Zn2+ ions. The introduction of Cl- inhibited the removal of Hg2+ due to the formation of mercuric chlorides (removal efficiency reduced from 97.8 to 95.6%). The removal efficiency of up to 86.7% was obtained after four cycles. The Langmuir isotherm and Pseudo-second kinetic were more suitable for fitting the adsorption process of Hg2+ by Zr(NH2)-DMP. The main removal mechanism could be attributed to the chelation between Hg2+ (soft acid) and nitrogen/sulfur (soft base) elements. These findings convinced that the successful synthesis of Zr(NH2)-DMP provides an option for Hg2+ removal from wastewater.

Effect of Different Placement Sequences of Water on the Methane Adsorption Properties of Coal.

After the coal seam is injected with water, the moisture content in the coal body increases, which affects the output capacity of coalbed methane (CBM). In order to improve the effect of CBM mining, the classical anthracite molecular model has been selected. To analyze the influence of different placement orders of water and methane on the characteristics of coal-adsorbing methane from the micro point of view, a molecular simulation method is used for comprehensive consideration in the study. The results show that H2O does not change the mechanism of CH4 adsorption by anthracite, but it inhibits the adsorption of methane by anthracite. When water enters the system afterward, there arises an equilibrium pressure point where water plays the most significant role in inhibiting methane adsorption by anthracite coals, which increases with increasing moisture content. When water enters the system first, no equilibrium pressure point occurs. The excess adsorption of methane by anthracite when water enters second is higher. The reason is that H2O can replace CH4 at the higher energy adsorption sites of the anthracite structure, while CH4 can only be adsorbed at the lower energy sites, and some of CH4 is not adsorbed. For the coal samples with a low-moisture content system, the equivalent heat of adsorption of CH4 increases first rapidly and then slowly with the increase of pressure. However, it decreases with pressure in the high-moisture content system. The variation of the equivalent heat of adsorption further explains the variation of the magnitude of methane adsorption under different conditions.

The role of blood podoplanin in patients with viral myocarditis.

Podoplanin (PDPN), a small mucin-like glycoprotein, was recently found to promote the generation of cardiac ectopic lymphoid follicles and anti-heart autoantibodies (AHA) in viral myocarditis (VMC) mice. Herein, we investigated the blood PDPN expression and its potential clinical value in VMC patients. Overall, 40 VMC patients were enrolled among 112 hospitalized patients with suspected myocarditis. Their serum PDPN levels were higher than those in controlled acute myocardial infarction (AMI) patients (n = 40) and healthy individuals (n = 30) (both p < 0.01) and positively correlated with CRP, IL-17, and IL-4 (all p < 0.01). Elevation of serum PDPN discriminated VMC from AMI (OR = 4.061, p < 0.01) and PDPN addition to the basic model (age, CRP, and peak cTNI) increased AUC values (from 0.822 to 0.933, p = 0.04). Additionally, the serum levels of PDPN ligand CCL-21 were also increased and correlated with PDPN (R = 0.59, p < 0.01) in VMC patients, accompanied by AHA production. Moreover, the anti-MHC antibody was closely related to PDPN levels (R = 0.53, p < 0.01), and anti-MHC-positive patients with VMC displayed higher percentages of CD4+IL-17A+PDPN+T cells and CD19+CCR7+B cells (both p < 0.05). Noticeably, VMC patients complicated by ventricular arrhythmias (27.50%) presented with AHA production and higher PDPN levels (p < 0.05). Finally, we screened out and verified that miR-182-5p directly targeted PDPN and negatively regulated its expression (all p < 0.01). These data suggested that blood PDPN might be a novel inflammation-associated biomarker for the early diagnosis of VMC and may contribute to AHA production by binding CCL-21 to recruit Th17 and B cells, which were regulated by miR-182-5p.

Commercial SCR catalyst modified with Cu metal to simultaneously efficiently remove NO and toluene in the fuel gas.

Developing an environmentally friendly selective catalytic reduction (SCR) catalyst to effectively eliminate both nitric oxides (NO) and toluene has garnered significant attention for regulating emissions from automobiles and the combustion of fossil fuels. This study synthesized a series of novel commercial V2O5-WO3/TiO2 catalysts modified with Cu through the wet impregnation method, which was employed to simultaneously remove NO and toluene from the fuel gas. The assessment of catalyst removal performance was conducted at a selective catalytic reduction system, and the experimental results showed a significant increase in the catalytic activity due to the modification of the copper metal. The 10% Cu/SCR catalyst showed a superior activity that the NO and toluene conversion reached 100% and 95.56% at 300 °C, respectively. Subsequently, various characterization techniques were employed to investigate the crystal phase, morphology, physical features, chemical states, and surface acidity properties of the synthesis catalysts. According to the characterization results, the presence of Cu metal did not have a noticeable impact on the physical property. However, the redox performance was enhanced, and the number of surface acidic sites was also increased after adding Cu to the SCR catalyst. Furthermore, the redox cycle of Cu metal and V species was facilitated to produce more active oxygen which helped to improve the NO and toluene conversion. This work offered a novel perspective into the synergistic oxidation of both NO and toluene, which was potentially relevant for improving the selective catalytic reduction process in coal-fired power plants.

The resin-supported iron-copper bimetallic composite as highly active heterogeneous Fenton-like catalysts for degradation of gaseous toluene.

In this study, a resin-supported iron-copper bimetallic heterogeneous Fenton catalyst with excellent removal performance, superior economy and outstanding recoverability was synthesized by an impregnation method and used to remove gaseous toluene. Experiments disclosed that 3-FeCu@LXQ-10 possessed extremely high catalytic capacity. At a temperature of 30 °C, an initial toluene concentration of 200 mg/m3 and H2O2 atomization amount of 3 mmol/h, the toluene removal efficiency of 3-FeCu@LXQ-10 was 97.50%. Experimental tests had revealed that the bimetallic supported catalysts exhibited higher catalytic activity than single metal-supported catalysts, owing to an interaction effect between iron and copper metal ions. Furthermore, electron paramagnetic resonance (EPR) and radical quenching tests were carried out, and the results indicated •OH radicals performed a key role in the Fenton-like process. In addition, the iron-copper bimetallic catalysts exhibited good reusability and stability characteristics during six degradation cycles. This study shows promising potential in using FeCu@LXQ-10 as a heterogeneous catalyst for removing toluene.

ACSL4 promotes ferroptosis and M1 macrophage polarization to regulate the tumorigenesis of nasopharyngeal carcinoma.

BACKGROUND: Nasopharyngeal carcinoma (NPC) is a head and neck malignant tumor with a high incidence and recurrence rate. The crosstalk between ferroptosis and tumor-associated macrophages (TAMs) is thought to have major implications in interfering with cancers. We intended to explore the effect of acyl-CoA synthetase long-chain family member 4 (ACSL4) on the pathogenesis of NPC via ferroptosis and TAMs. METHODS: Differential genes in NPC patients were analyzed using publicly available databases, and the ferroptosis-related gene ACSL4 was identified. Expression of ACSL4 in NPC cell lines and xenografted mice was examined. Colony formation, cell proliferation, migration, and invasion were assessed. The abundance of epithelial-mesenchymal transition (EMT) markers (E-cadherin, N-cadherin, and Vimentin) was confirmed. Lipid peroxidation levels and related markers were measured. Clophosome was administered to determine the role of TAMs in NPC mice. RESULTS: Low levels of ACSL4 were observed in NPC patients and CNE-2 and 5-8F cells. Erastin (a ferroptosis inducer) and ACSL4 increased lipid peroxidation, decreased cell viability, colony formation, cell proliferation, migration and invasion, and inhibited EMT. Moreover, Erastin and ACSL4 promoted M2 to M1 macrophage polarization. The effects of erastin and ACSL4 were additive. Ferrostatin-1, an inhibitor of ferroptosis, exerted the opposite effect and reversed the beneficial effects of ACSL4 overexpression. In xenograft mice, ACSL4 and clophosome hindered the growth of NPC, and extra clophosome slightly enhanced the antitumor effect of ACSL4. CONCLUSION: Our findings indicated that ACSL4 inhibited the pathogenesis of NPC, at least through crosstalk between ferroptosis and macrophages, providing potential direction for NPC therapy.

USP7 inhibits the progression of nasopharyngeal carcinoma via promoting SPLUNC1-mediated M1 macrophage polarization through TRIM24.

Reprogramming of macrophages toward an M1 phenotype is a novel strategy to induce anticancer immunity. However, the regulatory mechanisms of M1 macrophage polarization and its functional roles in nasopharyngeal carcinoma (NPC) progression need to be further explored. Here we found that SPLUNC1 was highly expressed and responsible for M1 macrophage polarization. JAK/STATs pathway activation was involved in SPLUNC1-mediated M1 macrophage polarization. Importantly, regulation of SPLUNC1 in macrophages affected CM-mediated influence on NPC cell proliferation and migration. Mechanistically, USP7 deubiquitinated and stabilized TRIM24, which promoted SPLUNC1 expression via recruitment of STAT3 in M1 macrophages. Depletion of TRIM24 inhibited M1 macrophage polarization, which facilitated NPC cell growth and migration. However, over-expression of USP7 exhibited the opposite results and counteracted the tumorigenic effect of TRIM24 silencing. Finally, the growth and metastasis of NPC cells in vivo were repressed by USP7-induced M1 macrophage polarization via modulating TRIM24/SPLUNC1 axis. USP7 delayed NPC progression via promoting macrophage polarization toward M1 through regulating TRIM24/SPLUNC1 pathway, providing evidence for the development of effective antitumor immunotherapies for NPC.

Study on the Differences between Various Gas Injection Sources in the Process of Coal Seam Methane Replacement by Gas Injection.

To study the differences between various gas injection sources in the process of coal seam methane replacement, experimental research was conducted to study the influences of different gas injection sources and gas injection ratios on coal seam methane replacement by considering the strong adsorption gas CO2 and the weak adsorption gas N2. The experimental results show that the temperature rise effect of the CO2 injection gas is greater and lasts longer than that of the N2 injection gas in the process of coal seam methane replacement by gas injection, and the temperature rise can promote the desorption of adsorbed methane well. However, due to the different effects of gas compressibility, the increase in gas pressure caused by injecting N2 under the same conditions is higher than that of CO2; due to the strong adsorption of CO2, the gas pressure after injecting CO2 continues to decrease slowly. From the displacement effect, the adsorbability of the injection source gas has a significant effect on the displacement rate and injection-placement ratio, while the injection ratio has a significant effect on CO2 displacement but not on N2 injection. From the experimental results, for N2 injection, the effects of coal seam methane replacement by gas injection can be enhanced by improving the gas injection method and process; for CO2 injection, the effects of coal seam methane replacement by gas injection can be improved by the following two aspects: gas injection volume and gas injection time.

Study on the Response Characteristics of the Pressure and Temperature Fields of Source Gas Injection under Loading Conditions.

To study the safety of the source gas in coal seams after gas injection displacement, choosing N2 as an example, the pressure and temperature field variation characteristics of the source gas in coal were studied via experimental research, numerical simulation, and theoretical analysis methods, starting from the variation characteristics of the gas pressure and temperature fields in the coal. In this work, 2-9 MPa stress was applied to the top of the coal samples. The experimental results indicated that during the coal loading process, the gas pressure and temperature in the coal generally exhibited a decreasing trend and that the pressure and temperature could increase in some areas due to pore closure and frictional heat generation, but the increase range was not significant. Based on the numerical simulation results, in the 200 min loading process, the gas pressure inside the coal body decreased by approximately 0.25 MPa and the overall temperature slightly decreased. Only the temperature near the borehole greatly changed, and the maximum decrease reached approximately 8 °C. Considering the experimental and numerical simulation results, under the condition of stress concentration, the pressure and temperature fields of the source gas injected into the coal body mainly revealed a decreasing trend and the possibility of inducing outbursts was low.