RESUMO
Cyclophilins (CyPs) are a family of proteins that bind the immunosuppressive agent cyclosporin A (CsA) with high-affinity and belong to one of the three superfamilies of peptidyl-prolyl cis-trans isomerases (PPIase). In this report, three cyclophilin genes (Ca-CyPs), including Ca-CyPA, Ca-CyPB and Ca-PPIL3, were identified from oyster, Crassostrea ariakensis Gould in which Ca-CyPA encodes a protein with 165 amino acid sequences, Ca-CyPB encodes a protein with 217 amino acid sequences and Ca-PPIL3 encodes a protein with 162 amino acid sequences. All of the three Ca-CyPs genes contain a typical CyP-PPIase domain with its signature sequences and Ca-CyPB contains an N-signal peptide sequences. Tissue distribution study revealed that Ca-CyPs were ubiquitously expressed in all examined tissues and the highest levels were observed in hemocytes. RLO incubation upregulated the mRNA expression levels of Ca-CyPs, indicating that three Ca-CyPs might be involved in oyster immune response against RLO infection.
Assuntos
Crassostrea/genética , Crassostrea/imunologia , Ciclofilinas/genética , Regulação da Expressão Gênica , Imunidade Inata , Sequência de Aminoácidos , Animais , Clonagem Molecular , Ciclofilinas/química , Ciclofilinas/metabolismo , DNA Complementar/genética , DNA Complementar/metabolismo , Sinais Direcionadores de Proteínas , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Alinhamento de Sequência/veterináriaRESUMO
LPS-induced TNFα factor (LITAF) is a transcription factor mediating TNF-α expression under LPS stimulation, and playing important roles in immune responses. In the present study, partial cDNA sequence of a LITAF (designated CcLITAF) gene was cloned and identified from snail Cipangopaludina chinensis. It contains an open reading frame of 348 nucleotides encoding a predicted protein of 115 amino acids, with a conserved LITAF domain at C-terminal, and shares a similarity ranging from 34% to 96% with other LITAF from oyster to mammals. CcLITAF mRNA ubiquitously expressed in all analyzed tissues. Interestingly, cLITAF could induce apoptosis in human tumor cell line, NCI-H446 cells, and caspase 3 play key roles in CcLITAF-mediated apoptosis. Present studies provide new insight into the biological function of CcLITAF.
Assuntos
Apoptose/efeitos dos fármacos , Caramujos/genética , Caramujos/metabolismo , Fator de Necrose Tumoral alfa/genética , Fator de Necrose Tumoral alfa/farmacologia , Sequência de Aminoácidos , Animais , Sequência de Bases , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Clonagem Molecular , Perfilação da Expressão Gênica , Humanos , Lipopolissacarídeos , Dados de Sequência Molecular , Filogenia , Proteínas Recombinantes/farmacologia , Proteínas Recombinantes/toxicidade , Alinhamento de Sequência , Caramujos/classificação , Fator de Necrose Tumoral alfa/química , Fator de Necrose Tumoral alfa/metabolismo , Fator de Necrose Tumoral alfa/toxicidadeRESUMO
The genus Unio is one of the widespread freshwater bivalves. To date, its intra-generic phylogeny remains controversial and therefore the mitochondrial genome data is needed. Here, we report the complete mitogenome of Unio douglasiae MG that is distributed in the Chihe River, a branch of Huaihe River, East China. This mitochondrial genome is 15,764 base pair in total length. It consists of 37 genes: 13 protein-coding genes, 22 tRNA genes, and 2 rRNA genes (12S and 16S). The base composition was 38.38% for A, 26.48% for T, 23.17% for C, and 11.98% for G, showing an obvious bias of higher A + T content (64.86%) than the G + C content (35.14%). Phylogenetic analysis showed that U. douglasiae MG is clustered with other Unio and Nodularia mollusks in the family Unionidae. These results showed that combine with morphological techniques, the mitogenome can provide useful information to further understanding of the genetics, systematics, and conservation of this endangered species.
RESUMO
In the present study, the complete mitogenome sequence of a Acanthorhodeus chankaensis Dybowsky from Cao'e River was sequenced and identified. The assembled mitogenome of A. chankaensis is 16,676 bp in length, it contains 22 transfer-RNA genes, 13 protein-coding genes, 2 ribosomal-RNA genes, and 2 non-coding regions. It shows conserved gene arrangement with other Cyprinidae fishes. The overall nucleotide composition of A. chankaensis mitogenome sequence is A: 28.96%, G: 17.11%, T: 27.46%, and C: 26.47%. The phylogenetic analysis showed that the complete mitogenome could contribute to the phylogenetic analyses and population genetics study of A. chankaensis and Acanthorhodeus fish.
RESUMO
The complete mitochondrial genome of Hemibarbus labeo BML (Cypriniformes: Cyprinidae) is 16,612 bases in length. It consists of 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 1 non-coding region. Its overall nucleotide composition is A: 29.85%, G: 17.07%, T: 25.86%, and C: 27.23%, respectively, with an A + T rich feature (55.71%). The gene arrangement and organization of the mitogenome from H. labeo BML were very similar to other Cyprinidae fishes. The phylogenetic analysis showed that H. labeo BML clustered in genus Hemibarbus. These results will contribute to the taxonomy and conservation biology studies of H. labeo.
RESUMO
Northern snakehead, Channa argus, is a commercially important food fish species in China. In the present study, the complete mitochondrial genome of C.argus from the Baima Hu Lake was characterized. It is 16,558 bp in length, consist of 22 tRNA genes, 13 PCD genes, 2 rRNA genes, and 1 D-loop region. The overall base composition of the C. argus mitogenome is 27.26% A, 24.21% T, 31.58% C and 16.95% G, exhibits a similar AT bias (51.47%) feature to other vertebrate mitogenomes. The phylogenetic analysis showed that C. argus clustered in genus Channa. The present resultes provide useful information to population genetics and conservation biology studies of Channa fishes.
RESUMO
Solenaia oleivora, belongs to Bivalvia, Unionidae, and Gonideinae, is a burrowing bivalve uniquely distributed in China. In this study, the complete mitochondrial genome of S. oleivora MG was sequenced and determined. The complete mitogenome of S. oleivora MG is 16,392 bp in total length, consist of 22 tRNA genes, 13 protein-coding genes (PCGs), and 2 rRNA genes. The overall base composition of the S. oleivora MG mitogenome is 36.90% A, 23.85% T, 27.09% C, and 12.16% G, respectively, exhibits a similar AT bias (60.75%) feature to other invertebrate bivalve mitogenomes. The phylogenetic analysis that S. oleivora MG clustered in genus Solenaia. This result provides useful data to the conservation and sustainable utilization of S. oleivora MG and other invertebrate mussels.
RESUMO
Sarcocheilichthys sinensis (Bleeker), is a small-sized benthopelagic fish with ornamental value. In the present study, the complete mitochondrial genome of S. sinensis was sequenced and determined. The complete mitogenome of S. sinensis was 16,683 bp in length, consisting of 22 tRNA genes, 13 protein-coding genes, 2 rRNA genes, and 2 non-coding regions. The overall base composition of the S. sinensis mitogenome is 30.50% A, 26.28% T, 26.60% C, and 16.61% G, exhibiting obvious AT bias (56.79%). The phylogenetic analysis showed that S. sinensis clustered in genus Sarcocheilichthys. Present study provides useful data to population genetics and conservation biology of Sarcocheilichthys fishes.
RESUMO
The BMP1/TLD-like proteinases are pleiotropic, astacin-like metalloproteinases. They play central roles in regulating the formation of the extracellular matrix (ECM) and signaling through various TGFbeta-like proteins in morphogenetic and homeostatic events. Here we describe the cloning, structural characterization and expression of Tolloid-like gene in the oyster, Crassostrea ariakensis (CaTLL). The full-length cDNA of CaTLL spans 3492 nucleotides including an open reading frame of 2811 nucleotides which encodes a hypothetical protein of 936 amino acids, with a molecular mass of approximately 103 kDa. The CaTLL molecule possessed structural features of several motifs including an N-terminal signal peptide sequence, a prodomain with an RTRR motif, an astacin-like domain that contains a conserved zinc-binding motif HELGHVIGFWHEH, five CUBs and two EGF domains with the arrangement CUB-CUB-EGF-CUB-EGF-CUB-CUB. The proteolytic domain of Ca-Tolloid shares more than 30% identity with other astacins of various animals from squail to mammals, indicating its conserved catalytic ability. RT-PCR and quantitative real-time PCR analyses revealed that CaTLL showed the lowest expression level in hemocytes of normal groups, but was affected significantly by the challenge of an obligate intracellular Gram-negative bacterium, Rickettsia-like organisms, suggesting that Ca-Tolloid might be involved in the molluscan immune response, and its function is more diverse than previously assumed.
Assuntos
Crassostrea/enzimologia , Crassostrea/genética , Regulação da Expressão Gênica , Metaloproteases Semelhantes a Toloide , Sequência de Aminoácidos , Animais , Clonagem Molecular , Dados de Sequência Molecular , Filogenia , Alinhamento de Sequência , Metaloproteases Semelhantes a Toloide/química , Metaloproteases Semelhantes a Toloide/genética , Metaloproteases Semelhantes a Toloide/metabolismoRESUMO
In this study, the complete mitochondrial genome sequence of a Xenocypris davidi from Cao'e River was sequenced. The complete mitogenome of X. davidi was 16,630 bp in length, it contains the structure of 22 transfer RNA genes, 13 protein coding genes, 2 ribosomal RNA genes, and 1 non-coding region. The gene arrangement and organization in the mitogenome of X. davidi were in accordance with other Cyprinidae fishes. The results of phylogenetic analysis revealed that the mitochondrial genome sequence could provide useful information for the conservation genetics and evolution study of X. davidi.
RESUMO
Hyriopsis cumingii is a freshwater pearl mussel in china, easily identified by a big sail-like bulging at back edge of the mussel. In the present study, we reported the complete mitochondrial genome of H. cumingii. It was 16,958 bp in length, and it contained 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes. Besides, we conducted alignment with other two different individuals of H. cumingii and phylogenetic analysis with closely related species.
Assuntos
Genoma Mitocondrial/genética , Unionidae/genética , Animais , DNA Complementar , Filogenia , RNA Ribossômico/genética , RNA de Transferência/genética , Unionidae/classificaçãoRESUMO
In the present study, the total mitochondrial genome of a freshwater mussel Cristaria plicata (Leach) was sequenced and determined. The mitogenome is 15,710 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes and 2 rRNA genes. The total base composition is: 36.49% A, 27.23% T, 23.21% C, and 13.07% G with an A+T rich feature (63.72%). These results provide useful data to study of the genetics, systematics and conservation of mussel C. plicata (Leach).
Assuntos
Genoma Mitocondrial , Mitocôndrias/genética , Unionidae/genética , Animais , Composição de Bases , Tamanho do Genoma , Análise de Sequência de DNA/métodosRESUMO
Sarcocheilichthys sinensis sinensis (Bleeker, 1871), is a small benthopelagic freshwater species with high nutritional and ornamental value. In this study, the complete mitochondrial genome of S. sinensis sinensis was determined; the phylogenetic analysis with another individual and closely related species of Sarcocheilichthys fishes was carried out. The complete mitogenome of S. sinensis sinensis was 16683 bp in length, consist of 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 2 non-coding regions: (D-loop and OL). It indicated that D-loop, ND2, and CytB may be appropriate molecular markers for studying population genetics and conservation biology of Sarcocheilichthys fishes.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial/fisiologia , Filogenia , Animais , Sequência de Bases , Proteínas de Peixes/genética , Proteínas Mitocondriais/genética , Dados de Sequência Molecular , RNA/genética , RNA Mitocondrial , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
Pseudobagrus ondon is a small benthopelagic freshwater species of family Bagridae. In the present study, the complete mitochondrial genome of P. Ondon has been determined, and phylogenetic analysis with another individual and closely related species are preformed. The complete mitochondrial genome of P. ondon was 16,534 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes and 2 non-coding regions: a control region (D-loop) and an L-strand replication origin (OL). It indicated that D-loop, ND1, ND2, ND4, ND5 and CYTB may be appropriate markers for studying population as well as conservation genetics.
Assuntos
Peixes-Gato/genética , Genoma Mitocondrial/genética , Análise de Sequência de DNA , Animais , Genes de RNAr/genética , Anotação de Sequência Molecular , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Filogenia , RNA de Transferência/genéticaRESUMO
In this study, the total mitochondrial genome sequence of Andrias davidianus (Caudata: Cryptobrachidae) was determined. The genome is 16,519 bases in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 2 non-coding regions. These results will contribute to the natural resources conservation and species identification Of A. davidianus.
RESUMO
The Chinese mitten crab Eriocheir sinensi belongs to the family Decapoda, Varunidae, Eriocheir. In this study, the full length of mitochondrial genome of an ovulated Chinese mitten crab was first sequenced and analyzed. It was determined to be 16,353 bp in length, and consists of 13 protein coding genes (PCDs), 22 tRNA genes, 2 rRNA genes and 1 control region.
Assuntos
Crustáceos/genética , Genoma Mitocondrial , Animais , Feminino , Masculino , Proteínas/genética , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
In the present study, the total mitochondrial genome of a precocious Chinese mitten crab Eriocheir sinensis was firstly determined. The genome is 16,350 bp in length. It consists of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and a control region. The overall nucleotide composition of the mitochondrial genome is A: 35.31 %, G: 10.69 %, T: 36.37 % and C: 17.63%, respectively. These results provide useful information to species identification, conservation and analysis the mechanisms of precocity in E. sinensis.
Assuntos
Braquiúros/genética , Genoma Mitocondrial , Animais , Proteínas de Artrópodes/química , Proteínas de Artrópodes/genética , Composição de Bases , Códon de Iniciação , Códon de Terminação , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA Mitocondrial/metabolismo , Fases de Leitura Aberta/genética , RNA Ribossômico/química , RNA Ribossômico/genética , RNA de Transferência/química , RNA de Transferência/genética , Análise de Sequência de DNARESUMO
Hemibarbus is a genus of cyprinid fishes distributed in eastern Asia. In the present study, we report here the complete mitochondrial genome of the Hemibarbus sp.090914 (Cypriniformes: Cyprinidae). Our results show that the complete mitochondrial DNA of Hemibarbus sp.090914 is 16,610 bp in length, and predicted to encode all the 37 genes that are typical for the vertebrates.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , Composição de Bases/genética , Pareamento de Bases/genética , DNA Mitocondrial/genética , Genes Mitocondriais , RNA Ribossômico/genética , RNA de Transferência/genéticaRESUMO
The complete mitogenome sequence of Sarcocheilichthys parvus was identified using polymerase chain reaction and sequenced with primer walking method. The complete mitogenome was 16,674 bp in length, containing 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 2 non-coding regions. The gene arrangement and composition of S. parvus was similar to most other fishes.
Assuntos
Cyprinidae/genética , Genoma Mitocondrial , Animais , DNA Mitocondrial/química , DNA Mitocondrial/isolamento & purificação , DNA Mitocondrial/metabolismo , Proteínas de Peixes/química , Proteínas de Peixes/genética , Proteínas de Peixes/metabolismo , Reação em Cadeia da Polimerase , RNA Ribossômico/química , RNA Ribossômico/genética , RNA Ribossômico/metabolismo , RNA de Transferência/química , RNA de Transferência/genética , RNA de Transferência/metabolismo , Análise de Sequência de DNARESUMO
In the present study, the total mitochondrial genome of Microphysogobio tafangensis (Cypriniformes: Cyprinidae) was determined. The full genome was 16,683 bp in length, which consisted of 13 protein-coding genes, 22 tRNA genes, 2 rRNA genes and 2 non-coding regions. The nucleotide composition of M. tafangensis mitochondrial genome was A: 29.38%, G: 17.71%, T: 25. 47% and C: 27.44%.