Spatial frequency shift super-resolution imaging based on quasiperiodic grating and deep learning.

In this study, we propose a pioneering spatially frequency-shifted super-resolution microscopy technique that utilizes the synergy of quasiperiodic gratings and deep learning. First, a quasiperiodic grating capable of converting evanescent waves into propagating waves is designed. The grating is positioned between the object under investigation and the objective lens, and the high-frequency information carried by the evanescent waves in the near-field region of the object is shifted into the detection window and becomes accessible in the far field for imaging. Subsequently, we provide two deep learning models for image and video reconstructions to achieve the reconstruction of static and dynamic samples respectively. Simulation results demonstrate the high feasibility of the proposed method, and both static and dynamic objects with sub-wavelength features can be resolved. The developed method paves the way to the realization of super-resolution imaging by using a traditional bright-field microscope without the need for an extensive optical system design.

Surface plasmon-enhanced dark-field microsphere-assisted microscopy.

We present for the first time a surface plasmon-enhanced dark-field microsphere-assisted microscopy in imaging both low-contrast dielectric objects and metallic ones. We demonstrate, using an Al patch array as the substrate, the resolution and contrast in imaging low-contrast dielectric objects are improved compared to that of the metal plate substrate and a glass slide in dark-field microscopy (DFM). 365-nm-diameter hexagonally arranged SiO nanodots assembled on the three substrates can be resolved, with the contrast varied from 0.23 to 0.96, and the 300-nm-diameter hexagonally close-packed polystyrene nanoparticles can only be discerned on the Al patch array substrate. The resolution can be further improved by using the dark-field microsphere-assisted microscopy, and an Al nanodot array with a nanodot diameter of ∼65 nm and a center-to-center spacing of 125 nm can be just resolved, which cannot be distinguished in a conventional DFM. The focusing effect of the microsphere, as well as the excitation of the surface plasmons, provides evanescent illumination with enhanced local electric field (E-field) on an object. The enhanced local E-field acts as a near-field excitation source to enhance the scattering of the object, resulting in the improvement of imaging resolution.

Microsphere-assisted dark-field microscopy based on a fully immersed low refractive index microsphere.

Here we find that a fully immersed low refractive index SiO2 microsphere (or a microcylinder, a yeast cell) can clearly distinguish a sample with sub-diffraction features in dark-field illumination mode. The resolvable area of the sample by microsphere-assisted microscopy (MAM) is composed of two regions. One region locates below the microsphere, and a virtual image of this part of the sample is formed by the microsphere first and then the virtual image is received by the microscope. The other region is around the edge of the microsphere, and this part of the sample is directly imaged by the microscope. The simulated region of the enhanced electric field on the sample surface formed by the microsphere is consistent with the resolvable region in the experiment. Our studies show that the enhanced electric field on the sample surface generated by the fully immersed microsphere plays an important role in dark-field MAM imaging, and this finding will have a positive effect on exploring novel mechanisms in resolution improvement of MAM.

Super-resolution imaging of plasmonic nanostructures by microsphere-assisted microscopy.

We fabricate both triangularly and circularly shaped Au, Ag, and Cr nanoparticle arrays and observe the imaging properties of these plasmonic nanostructures by BaTiO3 glass (BTG) microsphere-assisted microscopy. We experimentally find that the resolution of triangularly shaped Ag nanoparticle arrays is higher than that of Au and Cr ones, and a gap resolution of ∼λ/7.7 is demonstrated for the circularly shaped Au, Ag, and Cr nanostructures. Numerical simulations show that when a fully immersed BTG microsphere is dispersed on the surface of a plasmonic nanostructure sample, an enhanced electric field is generated in the vicinity of the sample, especially at the gap of the microsphere and the sample, due to the focusing effect of the microsphere and the excitation of localized surface plasmon resonance in the plasmonic nanostructure. The enhanced electric field in Ag nanostructures is significantly stronger than that in Au and Cr ones. Besides, the microsphere collects, amplifies, and propagates the enhanced near-field information to the far field, resulting in the improvement of imaging resolution.

Direct observation Brownian motion of individual nanoparticles in water using microsphere-assisted microscopy.

Observing Brownian motion of nanoscale objects through a traditional optical microscope is still a challenge. Here, we present a method to overcome this challenge by using a traditional optical microscope assisted with a removable microsphere-embedded thin film. The diffusion coefficient of individual unconstrained polystyrene (PS) nanoparticles with a diameter of 300 nm in water is calculated from their respective mean-square displacement versus time curves, and the measured diffusion coefficient shows good agreement with the theoretical Stokes-Einstein one, proving the feasibility of our method. In addition, the experimental results show that the movement of the PS nanoparticles is slowed down near a plane wall, and the diffusion coefficient is consistent with the theoretical constrained diffusion coefficient, which shows that our method can also study the constrained Brownian motion of nanoparticles constrained near a plane wall. Our research results are helpful for the application of microsphere-assisted microscopy in new fields and also provide a new method for nanoparticle tracking.

Super-resolution imaging properties of cascaded microsphere lenses.

In this paper, the imaging properties of a cascaded microsphere lens are studied. The cascaded microsphere lens consists of two lenses. A hexagonally close-packed 960-nm-diameter array of polystyrene microspheres is used as the first lens. The second lens is a single silica microsphere with a diameter of about 5 µm. A Blu-ray disc is observed by both the cascaded microsphere lens and the single silica microsphere. Studies reveal that the magnification of the cascaded microsphere lens is about 1.4 times greater than that of the single silica microsphere, while the field of view of the cascaded microsphere lens, which is close to the diameter of the polystyrene microsphere, is decreased. The focal position of the cascaded lens microsphere must be close to the sample in order to observe it.

Reduced distortion in high-index microsphere imaging by partial immersion.

We experimentally demonstrate that pincushion distortion is obvious when using a BaTiO3 glass (BTG) microsphere fully immersed in ethanol or SU-8 2002 resist to image a Blu-ray disk with sub-diffraction features. The distortion is related to the layer thickness of the immersing medium. For a BTG microsphere partially immersed in the SU-8 resist where the SU-8 thickness is around 4/5 the diameter of the microsphere, its distortion decreases dramatically, but it can still clearly resolve the Blu-ray disk. For such a partially immersed microsphere, the calculated position of the photonic nanojet is outside the microsphere and close to the object, indicating the microsphere has a super-resolution imaging property, and the distortion simulated by ZEMAX is decreased.

Influence of the photonic nanojet of microspheres on microsphere imaging.

The imaging properties of BaTiO3 glass (BTG) microspheres in the diameter range of 5-50 µm which are fully immersed in a polydimethylsiloxane layer are experimentally studied. Our experimental results show that for both Blu-ray disc samples and the single-layer hexagonally close-packed microsphere array samples, with the increase of the diameter of BTG microspheres, the range of focal image positions (RFIP) increases linearly. When the diameter of BTG microspheres increases from 5 to 50 µm, the RFIP changes from 4 to 25 µm. For the microsphere array samples, Talbot effect is observed, and both the position of Talbot images and the Talbot distance depend on the diameter of BTG microspheres. Numerical simulations indicate that the length of the photonic nanojet changes from 2.9 to 7.1 µm when the BTG microsphere size increases from 5 to 50 µm, and the calculated RFIP is between 6 and 24 µm. The calculated RFIPs match well with the experimental ones. Our researches reveal that the RFIP depends on the length of the photonic nanojet of the BTG microsphere.

Experimental far-field imaging properties of a ~5-µm diameter spherical lens.

Microscale lenses are mostly used as near-sighted lenses. The far-field imaging properties of a microscale spherical lens, where the lens is spatially separated from the object, are experimentally studied. Our experimental results show that, for a blu-ray disc (an object) whose spacing is 300 nm, the lens can magnify the stripe patterns of the disc when the lens is spatially separated from the object. In the experimentally tested range (0-14 µm), all the magnified images are virtual images. When the distance is increased from 0 to 14 µm the magnification decreases from 1.47× to 1.20× and the field of view increases from 3.8 to 12.2 µm. The image magnification cannot be described by standard geometrical optics.

Gravity-assisted convective assembly of centimeter-sized uniform two-dimensional colloidal crystals.

We have developed an inexpensive, robust, and easily controlled method, a gravity-assisted convective self-assembly method, to fabricate centimeter-sized uniform two-dimensional colloidal crystals. In this method, centimeter-sized two-dimensional colloidal crystals can be formed when the suspension concentration is in the range of 0.32-2.5 wt %. Once the ordering process starts, the formation of two-dimensional colloidal crystals is not affected when the environmental temperature gradually increases from 3 to 10 °C. Experimental results indicate that, in this method, gravity plays an important role in the colloidal crystal formation. The colloidal particles are transported to the edge of the suspension-glass interface, and the extra particles can be eventually moved to the edge of the slide by gravity.

Mimicking bicolor by changing the reflectance of the substrate in a one-dimensional periodic structure.

In nature, some beetles can display bicolor on their elytra. In order to explore the bicolor mechanism, we experimentally studied the optical and structural properties of the Carabus lafossei beetle. We found a multilayer structure in the cuticle of the beetle. Due to the different multilayer thicknesses in different areas, the beetle displayed bicolor. Here, we provide another approach to fabricate bicolor by depositing the same multilayer stack on a substrate with different reflectances at different areas. In this paper, the substrate with different reflectances is achieved by prefabricating sculpted hexagons (SU-8) on a silicon substrate. By coating a (ZnS/MgF2)3.5 multilayer, the sculpted structure displays green color at the ridges (SU-8/silicon area) and yellow color at the basins (silicon area).

Tunable figure of merit for a negative-index metamaterial with a sandwich configuration.

Metamaterials with a free-standing Ag-SU-8-Ag sandwich configuration, perforated with a periodic array of cross-dipole apertures, are fabricated. The transmittance spectra of both experiment and simulation have indicated the existence of two transmission bands in mid-IR frequencies. The high-frequency transmission peak is the guided mode associated with the surface plasmon polariton (SPP), and its location strongly depends on the arm length of the cross-dipole. The lower-frequency transmission peak is proposed to be the antisymmetric plasmon polariton mode, and its location hardly shifts as the size of the cross-dipole varies. Numerical simulations indicate that, on increasing the arm length of the cross-dipole aperture, the coaction of the electric dipole-like mode and the SPP-associated guided mode results in a better figure of merit.

A Multilayer Emitter Close to Ideal Solar Reflectance for Efficient Daytime Radiative Cooling.

A passive radiative cooling method has a significant influence on thermal management applications because it can cool without any energy input. This work both experimentally and theoretically demonstrates a multilayer thin film structure with high solar reflectance, which can be applied to passive daytime radiative cooling. The combination of physical vapor deposition and spin-coating prepared the samples, which were also characterized experimentally by spectrometers. On-site measured results show that the emitter can effectively achieve daytime radiative cooling, and the cooling performance can be further improved with the increase of the ambient air temperature. When the emitter is exposed to direct solar radiation (AM1.5) of about 880 W/m2 on a rooftop under dry air conditions, it can achieve an average temperature reduction of about 12.6 °C from the ambient air temperature with nonradiative heat transfer (11 a.m.-1 p.m.). Theoretical simulations reveal that the emitter can still have a certain cooling performance in the presence of significant nonradiative heat exchange and nonideal atmospheric conditions. The influence of ambient air temperature on the cooling performance of the emitter is also theoretically analyzed.

Finite-size effect on one-dimensional coupled-resonator optical waveguides.

We study the finite-size effect on the dispersion relation, group velocity, and transmission curves of one-dimensional finite-size coupled-resonator optical waveguide (CROW) structures. Both the dispersion relation and the group velocity curves of a finite-size CROW oscillate along those of the corresponding infinite-extended ones. The oscillations can be suppressed by matching the equivalent admittance of the surrounding medium to that of the unit cell. Thelen's method is used to find the parameters of the matching layer to reduce oscillations on the group velocity and transmission spectra, and to analyze the structure parameters that determine the bandwidth and the group velocity.

[Factors affecting the determination of the percent carboxyhemoglobin saturation of blood].

OBJECTIVE: To investigate factors affecting the determination of the percent carboxyhemoglobin saturation (HbCO%) of blood in an attempt to offer further data for results interpretation and sample storage requirement. METHODS: The HbCO% of blood samples stored in various conditions were detected by three spectrophotometries during the succeeding 30 days. RESULTS: The data detected by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry were more stable than mono-wavelength spectrophotometry. The HbCO% of blood was significantly related with the storage conditions which include temperature, time and the degree of exposure to air. CONCLUSION: Determinations of HbCO% are reliable which performed by reductive double-wavelength spectrophotometry and double-wavelength spectrophotometry, combine with spectral scans. During 30 days, blood stored at 4 degrees C exposed to a limited volume of air does not influence the determination of HbCO%.

Experimental imaging properties of immersion microscale spherical lenses.

Using the immersion lensing technique, the resolution of a conventional spherical lens can be improved by a factor of 1/n over its value in air (n, the refractive index of the immersion medium). Depending on the relative position between an object and a lens, either a real or a virtual image is formed. Here we report a new physical phenomenon experimentally observed in the microscale lens imaging. We find that when a microscale spherical lens is semi-immersed in a medium, the resolution of the lens is improved as it can intercept more fine details of the object. However, the microscale lens has two image channels for the fine and coarse details and two images corresponding to the two components can be formed simultaneously. Our findings will advance the understanding of the super-resolution imaging mechanisms in microscale lenses.

Role of shape in middle-infrared transmission enhancement through periodically perforated metal films.

We report experimental results on the role of aperture shape in middle-infrared enhanced transmission through a metal film perforated with a periodic aperture array. The transmission spectrum is highly dependent on the aperture shape. When the aperture shape changes from circular to cross-dipole or the cross-dipole arm length increases, the transmission spectrum exhibits a large redshift. The enhanced transmission is proposed to be an interplay of surface plasmon polaritions at the metal surface and localized surface plasmons (LSPs) inside the apertures. The shift of the transmission bands is proposed to be due to the redshift of the LSP resonance spectrum of the individual apertures. The role of shape in enhanced transmission in the cascaded structure is also studied.

Enhanced light transmission through cascaded metal films perforated with periodic hole arrays.

We report experimental results on enhanced light transmission through two periodically perforated metal films separated by a layer of dielectric. A perforated metal film (single metallic structure) exhibits extraordinary optical transmission, and when two such perforated metal films are spaced by a dielectric layer (cascaded metallic structure), the transmission is further increased. The maximum transmission of the cascaded metallic structure, which depends on the distance between the two metal films, can be more than 400% greater than that of a corresponding single metallic structure. It is proposed that the coupling of surface plasmon polaritons between the two metal films is involved in the process.

Modified spontaneous emission of CdTe quantum dots inside a photonic crystal.

The angular dependence of the spontaneous emission of CdTe quantum dots (QDs) inside a photonic crystal with a pseudogap is reported. The sensitive dependences of the radiative lifetime and the photoluminescence spectrum of CdTe QDs on the observation angle demonstrate the effect of the photonic bandgap on the spontaneous emission of the QDs.