RESUMO
Fusion between bilayers of mixed egg phosphatidylcholine and soybean phosphatidylethanolamine was induced by freezing and thawing. Contact points between bilayers were observed by freeze fracture electron microscopy, and isotropic molecular motional averaging was detected by phosphorus-31 nuclear magnetic resonance under fusion conditions. A molecular model of point defect structure is proposed as an intermediate stage of fusion.
Assuntos
Bicamadas Lipídicas , Membranas/fisiologia , Técnica de Fratura por Congelamento , Modelos Biológicos , Fosfatidilcolinas , FosfatidiletanolaminasRESUMO
Recent studies concerning cholesterol, its behavior and its roles in cell growth provide important new clues to the role of this fascinating molecule in normal and pathological states.
Assuntos
Membrana Celular/metabolismo , Colesterol/fisiologia , Lipídeos de Membrana/fisiologia , Animais , Transporte Biológico , Membrana Celular/ultraestrutura , Permeabilidade da Membrana Celular , Colesterol/metabolismo , Cristalização , Filipina/metabolismo , Regulação da Expressão Gênica , Hidroximetilglutaril-CoA Redutases/metabolismo , Mamíferos/metabolismo , Fluidez de Membrana , Lipídeos de Membrana/metabolismo , Proteínas de Membrana/metabolismo , Conformação Molecular , Fosfolipídeos/metabolismo , Esfingolipídeos/metabolismo , Esteróis/metabolismo , TermodinâmicaRESUMO
2H nuclear magnetic resonance (2H-NMR) spectra of dioleoylphosphatidylcholine labelled at positions 9 and 10 in the acyl chains of the phospholipid were obtained in the presence of cholesterol and lanosterol. The spectra show in all cases three quadrupole splittings. One is due to the deuterium on position 10 of the sn-1 chain and another to the deuterium on position 10 of the sn-2 chain. The third deuterium quadrupole splitting arises from the deuterium at position 9 of both chains. Cholesterol, at increasing concentration, produces an increase in the quadrupole splitting from position 9, corresponding to an increase in order of that C-D bond segment arising from the inclusion of cholesterol in the membrane. Little effect is noted on the quadrupole splittings arising from position 10 of either chain. Lanosterol appears to have no effect on the quadrupole splittings from position 9. Lanosterol, likewise, has no effects on the quadrupole splittings from position 10 of both chains. These data therefore suggest little disorganization of the membrane structure due to the 14-methyl group. However, the 14-methyl group prevents lanosterol from causing the increase in motional order of the phospholipid hydrocarbon chains characteristic of cholesterol.
Assuntos
Colesterol , Lanosterol , Fosfolipídeos , Fenômenos Químicos , Físico-Química , Espectroscopia de Ressonância Magnética , FosfatidilcolinasRESUMO
The cholesterol content of human erythrocyte membranes has been modified by incubation of intact cells with sonicated egg phosphatidylcholine/cholesterol vesicles and with egg phosphatidylcholine vesicles. (Na+ + K+)-ATPase ATP hydrolyzing activity was measured as a function of membrane cholesterol content. High membrane cholesterol inhibits the ATPase activity of the enzyme and low membrane cholesterol activates that enzyme activity. The most likely mechanism of inhibition is suggested to comprise direct cholesterol-protein interactions which lead to a low activity conformation. Ouabain binding studies show that the inhibition is not due to a loss of enzyme from the membrane.
Assuntos
Colesterol/sangue , Membrana Eritrocítica/enzimologia , Eritrócitos/enzimologia , Lipídeos de Membrana/sangue , ATPase Trocadora de Sódio-Potássio/sangue , Adulto , Colesterol/farmacologia , Humanos , Cinética , Lipossomos , Ouabaína/sangue , Fosfatidilcolinas/farmacologia , Receptores de Droga/metabolismoRESUMO
13C-NMR spectra of cholesterol 90% enriched at C-4 with 13C have been obtained in CHCl3 and in sonicated egg phosphatidylcholine vesicles. 13C spin-lattice relaxation times, nuclear Overhauser effects and spin-spin relaxation times have been measured for the C-4 carbon of cholesterol in phosphatidylcholine bilayers as a function of cholesterol content and temperature. All the data are consistent with a correlation time for axial rotation of about 10(-10) s. This rotation is one or two orders of magnitude faster than axial rotation of the phospholipid molecule.
Assuntos
Colesterol , Bicamadas Lipídicas , Fosfatidilcolinas , Espectroscopia de Ressonância Magnética , Matemática , Conformação MolecularRESUMO
The phase behavior of L-alpha-dimyristoylphosphatidylcholine/cholesterol mixtures was studied in multilamellar vesicles by fluorescence polarization of the sterol molecule dehydroergosterol and of the polyene molecule alpha-parinaric acid. In the absence of cholesterol, dehydroergosterol exhibited an increase in polarization as DMPC vesicles were heated through the phase transition. This rise in polarization anisotropy was observed over a 0.6-1.0 degrees C increase in temperature with the midpoint of the phase transition occurring at 23.6 degrees C. Addition of 5 mol% cholesterol completely obliterated this change in polarization anisotropy through the phase transition of DMPC. alpha-Parinaric acid underwent a characteristic decrease in polarization anisotropy through the phase transition of DMPC. The change in anisotropy through the phase transition was over 4-fold greater than the values observed with dehydroergosterol. Vesicles containing 5 mol% cholesterol in the presence of alpha-parinaric acid underwent a decrease in polarization anisotropy that was over 75% of the original decrease in amplitude observed in the absence of any membrane cholesterol. The difference in sensitivity of the two fluorescent probes to the phase transition of DMPC as a function of membrane cholesterol content may be explained by a preferential partitioning of dehydroergosterol (and cholesterol) into a sterol-rich phase at low sterol concentrations. This partitioning allows dehydroergosterol to detect sterol-rich regions in the membrane bilayer.
Assuntos
Colesterol , Dimiristoilfosfatidilcolina , Fenômenos Químicos , Físico-Química , Ergosterol/análogos & derivados , Polarização de Fluorescência , Bicamadas Lipídicas , Espectrometria de FluorescênciaRESUMO
Motional order and motional rates in unsonicated phospholipid bilayers were assessed as a function of unsaturation of the phospholipid. A measurement sensitive to motional order was obtained using 2H-NMR of 18:1, 18:1-phosphatidylcholine labelled at positions 9 and 10 with deuterium and included as a probe in phospholipid bilayers of interest at 10 mole percent. Spin lattice relaxation times from magic angle spinning 13C-NMR spectra of phospholipid dispersions of interest were used as a measure of motional rates. Measurements were made of phospholipid bilayers containing from 0 to 8 double bonds per molecule. No large effect of an increase in unsaturation was noted for the 2H-NMR quadrupole splittings or for the 13C-NMR spin lattice relaxation rate.
Assuntos
Ácidos Graxos Insaturados/análise , Bicamadas Lipídicas , Fosfatidilcolinas , Isótopos de Carbono , Deutério , Espectroscopia de Ressonância Magnética/métodos , Modelos Biológicos , Conformação MolecularRESUMO
Phosphorus nuclear magnetic resonance spectra of rabbit muscle light sarcoplasmic reticulum membranes consist of two overlapping resonances, one much broader than the other. The broad resonance arises from phospholipids motionally restricted, probably by association with the Ca2+-ATPase, while the narrow resonance arises from phospholipid only slightly perturbed by the presence of the protein. (Selinsky, B.S. and Yeagle, P.L. (1984) Biochemistry 23, 2281-2288). The rate of exchange between the two phospholipid domains represented by the resonances was determined by measuring the transfer of magnetization from the broad resonance to the narrow resonance. The rate of exchange of phospholipids from the restricted domain to the nonrestricted domain was determined to be 1 s-1.
Assuntos
Fosfolipídeos/metabolismo , Retículo Sarcoplasmático/metabolismo , Animais , Transporte Biológico Ativo , ATPases Transportadoras de Cálcio/metabolismo , Espectroscopia de Ressonância Magnética , Fósforo , CoelhosRESUMO
Surface properties of Sendai virus envelope membrane have been measured, using both biological and biophysical techniques. Both normal and trypsin-treated virus were studied. SDS gel electrophoresis showed cleavage of the F protein exclusively by trypsin. The major activity change was observed in the hemolysing activity which is an expression of F protein. Hemolysis was reduced to less than 10% of its value for intact virus. 31P nuclear magnetic resonance studies of the envelope surface of the native virus showed a highly restricted phospholipid headgroup environment. Interestingly, this restriction was relieved by treatment with trypsin. Thus these data suggest a role of the F protein of Sendai virus in tightly organizing the surface of the viral envelope membrane.
Assuntos
Fusão de Membrana , Membranas/fisiologia , Vírus da Parainfluenza 1 Humana/fisiologia , Hemólise , Espectroscopia de Ressonância Magnética , Fluidez de Membrana , Fosfolipídeos/fisiologia , Tripsina , Proteínas do Envelope Viral/fisiologia , Proteínas Virais de FusãoRESUMO
Spectroscopic studies were performed on the fluorescent sterol probes ergosta-5,7,9(11),22-tetraen-3 beta-ol (dehydroergosterol) and cholesta-5,7,9(11)-trien-3 beta-ol (cholestatrienol). In most isotropic solvents, these molecules exhibited a single lifetime near 300 ps. Fluorescence lifetimes in 2-propanol were independent of emission wavelength and independent of excitation wavelength. Excited state behavior of these probes appears relatively simple. In isotropic solvents, dehydroergosterol fluorescence emission underwent at most a small Stokes shift as solvent polarity was modified. Time-resolved anisotropy decays indicated that dehydroergosterol decay was monoexponential, with rotational correlation times dependent on solvent viscosity. When incorporated into L-alpha-dimyristoylphosphatidylcholine liposomes at a concentration of 0.9 mol%, dehydroergosterol fluorescence lifetime decreased at the phase transition of this phospholipid indicating that the sterol probe was detecting physical changes of the bulk phospholipids. Furthermore, total fluorescence decays and anisotropy decays were sensitive to the environment of the sterol. Dehydroergosterol and cholestatrienol are thus useful probes for monitoring sterol behavior in biological systems.
Assuntos
Colestenos , Ergosterol/análogos & derivados , Dimiristoilfosfatidilcolina , Cinética , Lipossomos , Espectrometria de Fluorescência , TermodinâmicaRESUMO
The recently reported crystal structure of bovine rhodopsin revealed a cytoplasmic helix (helix 8) in addition to the seven transmembrane helices. This domain is roughly perpendicular to the transmembrane bundle in the presence of an interface and may be a loop-like structure in the absence of an interface. Several studies carried out on this domain suggested that it might act as a conformational switch between the inactive and activated states of this G-protein coupled receptor (GPCR). These results raised the question whether helix 8 may be an important feature of other GPCRs as well. To explore this question, we determined the structure of a peptide representing the putative helix 8 domain in another receptor that belongs to the rhodopsin family of GPCRs, the human beta(2) adrenergic receptor (hbeta(2)AR), using two-dimensional (1)H nuclear magnetic resonance (NMR). The key results from this structural study are that the putative helix 8 domain is helical in detergent and in DMSO while in water this region is disordered; the conformation is therefore dependent upon the environment. Comparison of data from five GPCRs suggests that these observations may be generally important for GPCR structure and function.
Assuntos
Receptores Adrenérgicos beta 2/química , Sequência de Aminoácidos , Dicroísmo Circular , Humanos , Micelas , Modelos Moleculares , Dados de Sequência Molecular , Ressonância Magnética Nuclear Biomolecular , Fosforilcolina/química , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Receptores Adrenérgicos beta 2/genética , Receptores Acoplados a Proteínas G/genética , Água/químicaRESUMO
Bacteriorhodopsin is one of very few transmembrane proteins for which high resolution structures have been solved. The structure shows a bundle of seven helices connected by six turns. Some turns in proteins are stabilized by short range interactions and can behave as small domains. These observations suggest that peptides containing the sequence of the turns in a membrane protein such as bacteriorhodopsin may form stable turn structures in solution. To test this hypothesis, we determined the solution structure of three peptides each containing the sequence of one of the turns in bacteriorhodopsin. The solution structures of the peptides closely resemble the structures of the corresponding turns in the high resolution structures of the intact protein.
Assuntos
Bacteriorodopsinas/química , Halobacterium salinarum/química , Sequências Hélice-Volta-Hélice , Peptídeos/química , Sequência de Aminoácidos , Cristalização , Modelos Moleculares , Dados de Sequência Molecular , Ressonância Magnética Nuclear Biomolecular/métodos , Estrutura Secundária de Proteína , SoluçõesRESUMO
The phase behavior of bovine rod outer segment disk lipids has been investigated using freeze-fracture and 31P nuclear magnetic resonance (NMR) techniques. 31P-NMR spectra of isolated disk membranes were taken as a function of temperature between 25 degrees C and 45 degrees C. The 31P-NMR spectrum characteristic of phospholipid bilayers was observed at all temperatures both in the absence of Ca2+ and in the presence of 10 mM and 50 mM Ca2+. A similar study was performed on lipids isolated from the disk membranes. In the absence of Ca2+ only lamellar phase behavior was observed. In the presence of less than 10 mM Ca2+, however, there was a change in morphology to non-lamellar structures. Removal of the Ca2+ caused the system to reassume the lamellar form.
Assuntos
Cálcio/farmacologia , Bicamadas Lipídicas/metabolismo , Células Fotorreceptoras/metabolismo , Segmento Externo da Célula Bastonete/metabolismo , Animais , Bovinos , Membrana Celular/efeitos dos fármacos , Membrana Celular/metabolismo , Membrana Celular/ultraestrutura , Técnica de Fratura por Congelamento , Espectroscopia de Ressonância Magnética , Microscopia Eletrônica , Segmento Externo da Célula Bastonete/efeitos dos fármacos , Segmento Externo da Célula Bastonete/ultraestrutura , TemperaturaRESUMO
Upon introduction into phosphatidylcholine vesicles, the 13C magnetic resonance peaks of the aromatic resonances of tetracaine are broadened while the T1 relaxation times show little change. Addition of tetracaine to vesicles containing 30% cholesterol produces a similar broadening in the 13C NMR spectrum of tetracaine. Nuclear magnetic resonance parameters of phosphatidylcholine in vesicles which are unchanged by the addition of equimolar tetracaine include 13C T1 relaxation time and 31P linewidth, T1 relaxation time, and nuclear Overhauser effect enhancement. These results are interpreted as indicating a hydrophobic interaction between hydrocarbon portions of the anesthetic and phospholipid bilayer. The rotational correlation time of tetracaine about its long axis in the vesicles has been calculated from the 13C NMR spin lattice relaxation times to be about 10(-10.3) s and is unchanged by incorporation into the phospholipid bilayer. The positively charged ammonium group of tetracaine interacts with the negatively charged phosphate group of the vesicle lipids. Using shift reagents and 31P NMR, tetracaine has been shown to displace cations from the bilayer surface, and does not undergo fast flip-flop across the vesicle bilayer.
Assuntos
Anestésicos Locais/química , Biofísica/métodos , Fosfatidilcolinas/química , Tetracaína/química , Anestésicos Locais/análise , Animais , Cátions , Cromatografia , Bicamadas Lipídicas/química , Espectroscopia de Ressonância Magnética , Óvulo/metabolismo , Compostos de Amônio Quaternário/química , Tetracaína/análise , Fatores de Tempo , Água/químicaRESUMO
31P nuclear magnetic resonance spectroscopy (31P-NMR) was used to study phospholipid organization in hydrated preparations of N-methyl dioleoylphosphatidylethanolamine and a 'fusion peptide' with the sequence: FAGV-VLAGAALGVAAAAQI, which corresponds to the amino terminus of the F1 subunit of the membrane fusion protein of measles virus. These amino acids are believed to mediate syncytia formation, host-cell penetration and hemolysis by infectious virus. The presence of the peptide at 0.5 mole percent significantly facilitated the formation of isotropic 31P resonances. The effects at 1 mole percent peptide were substantially enhanced over the effects observed at 0.5 mole percent, leading to a decrease in the onset temperature of the formation of the isotropic 31P-NMR resonances by about 30 degrees C. The formation of such isotropic 31P-NMR resonances has been previously associated with an increased rate of fusion of large unilamellar vesicles composed of N-methyl dioleoylphosphatidylethanolamine. Enhanced fusion of octadecyl rhodamine-labelled Sendai virus with N-methyl dioleoylphosphatidylethanolamine large unilamellar vesicles was observed when the 'fusion peptide' was incorporated into the large unilamellar vesicles.
Assuntos
Lipossomos , Vírus do Sarampo/fisiologia , Fusão de Membrana/efeitos dos fármacos , Vírus da Parainfluenza 1 Humana/fisiologia , Fosfatidiletanolaminas/química , Proteínas Virais de Fusão/farmacologia , Sequência de Aminoácidos , Animais , Embrião de Galinha , Espectroscopia de Ressonância Magnética/métodos , Dados de Sequência Molecular , Vírus da Parainfluenza 1 Humana/efeitos dos fármacos , Termodinâmica , Proteínas Virais de Fusão/síntese químicaRESUMO
The peptide ZfFG is known to inhibit non-bilayer phase formation as well as vesicle-vesicle and viral fusion. In order to ascertain some of the properties or structural features of this peptide which were important for the inhibition of membrane fusion, the blocking group was transferred from the amino to the carboxyl end to make fFGOBz. The fFGOBz lowered the bilayer to hexagonal phase transition temperature of dielaidoylphosphatidylethanolamine and it promoted the formation of isotropic phases in monomethyldioleoylphosphatidylethanolamine. The promotion of non-bilayer phases by fFGOBz appeared to be enhanced by a charged terminal amino group as higher pH or formylation of the amino group both decreased the effectiveness of this peptide to induce formation of the hexagonal phase. With the monomethyldioleoylphosphatidylethanolamine, the fFGOBz also promoted vesicle leakage and fusion as measured by lipid intermixing. The fFGOBz did not inhibit the formation of lipid structures of high curvature, resulting from sonication of phosphatidylcholine, as did ZfFG. Thus, the effects of fFGOBz on membranes are in sharp contrast to those of ZfFG and more closely resemble the behaviour of larger fusion peptides corresponding to the amino-terminal segment of viral fusion proteins. Our results demonstrate that having the carbobenzoxy group on the amino-terminus of fFG is important for giving the peptide derivative the property of inhibiting membrane fusion.
Assuntos
Bicamadas Lipídicas/química , Fusão de Membrana/efeitos dos fármacos , Oligopeptídeos/farmacologia , Sequência de Aminoácidos , Varredura Diferencial de Calorimetria , Concentração de Íons de Hidrogênio , Espectroscopia de Ressonância Magnética , Modelos Biológicos , Dados de Sequência Molecular , Oligopeptídeos/síntese química , Oligopeptídeos/química , Fosfatidiletanolaminas/química , Sonicação , Temperatura , Proteínas Virais de Fusão/químicaRESUMO
Low resolution electron density maps have revealed the general orientation of the transmembrane helices of rhodopsin. However, high resolution structural information for the transmembrane domain of the G-protein-coupled receptor, rhodopsin, is as yet unavailable. In this study, a high resolution solution structure is reported for a 15 residue portion of the sixth transmembrane helix of rhodopsin (rhovih) as a free peptide. Helix 6 is one of the transmembrane helices of rhodopsin that contains a proline (amino acid residue 267) and the influence of this proline on the structure of this transmembrane domain was unknown. The structure obtained shows an alpha-helix through most of the sequence. The proline apparently induces only a modest distortion in the helix. Previously, the structure of the intradiskal loop connected to helix 6 was solved. The sequence of this loop contained five residues in common (residues 268-272) with the peptide reported here from the rhovih. The five residues in common between these two structures were superimposed to connect these two structures. The superposition showed a root mean square deviation of 0.2 A. Thus, this five residue sequence formed the same structure in both peptides, indicating that the structure of this region is governed primarily by short range interactions.
Assuntos
Rodopsina/química , Sequência de Aminoácidos , Humanos , Espectroscopia de Ressonância Magnética , Modelos Moleculares , Dados de Sequência Molecular , Fragmentos de Peptídeos/química , Fragmentos de Peptídeos/genética , Prolina/química , Estrutura Secundária de Proteína , Rodopsina/genéticaRESUMO
Cholesterol modulates the function of rhodopsin in the retinal rod outer segment (ROS) disk membranes. One mechanism for such modulation is cholesterol modulation of the properties of the membrane bilayer. This has been explored previously. Another possible mechanism is an interaction between the sterol and the protein, which has not been previously explored. In this study, the fluorescent sterol, cholestatrienol, was used to probe interactions between cholesterol and rhodopsin in bovine ROS disk membranes. Cholestatrienol was incorporated into the disk membranes by exchange from donor phospholipid vesicles. Fluorescence energy transfer from protein tryptophans to cholestatrienol was observed indicating close approach of this fluorescent sterol to the tryptophan. The effectiveness of the energy transfer was measured by the quenching of tryptophan fluorescence by cholestatrienol. The quenching of tryptophan fluorescence was directly related to the cholestatrienol content of the membranes. Cholesterol was incorporated into the disk membranes by exchange from donor phospholipid vesicles. The effect of increasing membrane cholesterol on the ability of cholestatrienol to quench rhodopsin tryptophan fluorescence was determined. This quenching was inversely proportional to the membrane cholesterol content. Furthermore the observed quenching was greater than could be explained by a simple dilution of the cholestatrienol by the addition of cholesterol to the membrane. These data suggest an interaction between the sterol and the protein. The specificity of this interaction was explored by the addition of ergosterol, instead of cholesterol, to the disk membranes. Ergosterol was not able to inhibit the quenching of protein trytophans beyond that due to dilution of the cholestatrienol by addition of ergosterol to the membrane. The ability of cholesterol to compete with cholestatrienol for that interaction suggests a 'site' at which cholesterol contacts rhodopsin. The inability of ergosterol to compete with cholestatrienol for this 'site' suggested that the site was specific for the structure of cholesterol.
Assuntos
Colesterol/química , Rodopsina/química , Segmento Externo da Célula Bastonete/química , Animais , Bovinos , Sistema Livre de Células , Colestenos/química , Transferência de Energia , Membranas Intracelulares/química , Segmento Externo da Célula Bastonete/ultraestrutura , Espectrometria de Fluorescência , Triptofano/químicaRESUMO
The anti-viral and membrane fusion inhibitor, carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine (ZfFG), was studied in phospholipid bilayers, where earlier studies had indicated this peptide functioned. Multinuclear magnetic resonance (NMR) studies were performed with isotopically labeled peptide. A peptide labeled in the glycine carboxyl with 13C was synthesized, and the isotropic 13C-NMR chemical shift of that carbon was measured as a function of pH. A pKa of 3.6 for the carboxyl was determined from the peptide bound to a phosphatidylcholine bilayer. ZfFG inhibits the formation by sonication of highly curved, small unilamellar vesicles. Experiments as a function of pH revealed that this ability of ZfFG was governed by a pKa of 3.7. Therefore the protonation state of the carboxyl of ZfFG appeared to regulate the effectiveness of this anti-viral peptide at destabilizing highly curved phospholipid assemblies. Such destabilization had previously been discovered to be related to the mechanism of the anti-fusion and anti-viral activity of this peptide. The location of the carboxyl of ZfFG in the membrane was probed with paramagnetic relaxation enhancement of the 13C spin lattice relaxation of the carboxyl carbon in the glycine of ZfFG (enriched in 13C). Results suggested that this carboxyl is at or above the surface of the phospholipid bilayer. The dynamics of the molecule in the membrane were examined with 2H-NMR studies of ZfFG, deuterated in the alpha-carbon protons of the glycine. When ZfFG was bound to membranes of phosphatidylcholine, a sharp 2H-NMR spectral component was observed, consistent with a disordering of the glycine methylene segment of the peptide. When ZfFG was bound to N-methyl dioleoylphosphatidylethanolamine (N-methyl DOPE) bilayers at temperatures below 30 degrees C, a large quadrupole splitting was observed. These results suggest that ZfFG likely inhibits membrane fusion from the surface of the lipid bilayer, but not by forming a tight, stoichiometric complex with the phospholipids.
Assuntos
Antivirais/química , Bicamadas Lipídicas/química , Espectroscopia de Ressonância Magnética , Fusão de Membrana/efeitos dos fármacos , Oligopeptídeos/química , Fosfolipídeos/química , Sequência de Aminoácidos , Deutério , Glicina/química , Concentração de Íons de Hidrogênio , Dados de Sequência Molecular , Oligopeptídeos/metabolismo , Fosfatidilcolinas/metabolismo , Fosfatidiletanolaminas/metabolismo , Fosfolipídeos/metabolismo , Marcadores de SpinRESUMO
Aqueous dispersions of bovine brain sphingomyelin were studied as a function of temperature. 31P-NMR, X-ray diffraction, and negative-stain and freeze-fracture electron microscopy were used to determine the morphology and phase structure at several temperatures. 31P-NMR indicated a change in phase structure with an increase in temperature. Evidence was found only for the lamellar phase at all temperatures studied with X-ray diffraction. Electron microscopy unexpectedly revealed the spontaneous development of small unilamellar vesicles at elevated temperatures, consistent with the 31P-NMR data, in the absence of any outside disturbances.