RESUMO
BACKGROUND: There is a lack of published evidence for treatment and outcome measures for vulval erosive lichen planus (ELPV). OBJECTIVES: To conduct a multicentre case note review to examine real-life management of ELPV comparing current U.K. practice against an agreed audit standard. METHODS: Criteria for standards of care for which to evaluate current service provision were set following communication with experts from the British Society for the Study of Vulval Disease. Participants from 10 U.K. centres included nine dermatologists and one gynaecologist who run specialist vulval clinics. Standards examined the documentation of disease severity/impact measures, the use of diagnostic biopsies, treatments used and assessment of treatment response. RESULTS: Audit data were collected from 172 patients. Documentation of symptoms/clinical findings was excellent (99%, 170/172). A schematic diagram was present in the notes of 87% (150/172). Patient-related disease impact measures including Dermatology Life Quality Index (3%, 6/172) or visual analogue scales (1%, 2/172) were less well documented. Biopsies were performed in 78% (135/172); 71% (96/135) showed histological features consistent with erosive lichen planus. Squamous cell carcinoma developed in four patients (two vulval, two oral) and vulval intraepithelial neoplasia in two further patients. Recommended first-line treatment with a very potent topical steroid was used in 75% (129/172) with improvement in 66% (85/129). Significant variation in second-line therapy was seen. CONCLUSIONS: Wide variation in U.K. practice demonstrates the absence of standardized guidance for treating ELPV and the need for vulval-specific outcomes. This audit should act as a framework towards improving ELPV management and to plan future research in this area.
Assuntos
Atenção à Saúde/normas , Líquen Plano/terapia , Doenças da Vulva/terapia , Administração Cutânea , Administração Oral , Adulto , Idoso , Idoso de 80 Anos ou mais , Anti-Inflamatórios , Feminino , Humanos , Imunossupressores/administração & dosagem , Líquen Plano/diagnóstico , Auditoria Médica , Pessoa de Meia-Idade , Educação de Pacientes como Assunto , Guias de Prática Clínica como Assunto , Qualidade de Vida , Esteroides/administração & dosagem , Reino Unido , Doenças da Vulva/diagnósticoAssuntos
Granuloma Anular/patologia , Derme/patologia , Diagnóstico Diferencial , Humanos , Masculino , Pessoa de Meia-Idade , Ombro , TroncoRESUMO
Anti-52- and anti-60-kDa Ro/SS-A (Ro) autoantibodies are produced by most patients with subacute cutaneous lupus erythematosus and neonatal lupus erythematosus and are thought to be pathogenic in these two disorders. To learn more about the epidermal antigens targeted by Ro autoantibodies, a panel of anti-52 and anti-60-kDa Ro antibodies was purified from human autoimmune sera and rabbit antisera and then used to: (i) determine the expression and location of the Ro antigens in human keratinocytes; (ii) clarify discrepancies in previous localization studies; and (iii) verify the existence of Ro autoantibodies that cross-react with the 52- and 60-kDa Ro antigens, as previously reported. By immunoblot analysis these antibodies demonstrate that 52- and 60-kDa Ro proteins are expressed in normal human skin and cultured keratinocytes. By indirect immunofluorescence studies with cultured human cells, the anti-52-kDa Ro antibodies produce fine granular cytoplasmic fluorescence and less intense nuclear fluorescence, with apparent nucleolar sparing. The anti-60-kDa Ro autoantibodies produce weak cytoplasmic fluorescence and intense coarse granular nuclear fluorescence with apparent nucleolar sparing. We found distinct differences in the intracellular localization of the 52- and 60-kDa Ro autoantigens. This difference suggests that the 52-and 60-kDa Ro antigens may have independent cellular functions. Finding 60-kDa Ro antigen predominantly in the nucleus challenges the notion that the majority of the intracellular 60-kDa Ro antigen is complexed with the cytoplasmic hY RNA. Additionally, our failure to find a cross-reactive epitope on these two proteins indicates that the 52-kDa Ro antigen is probably a true immunogen and not merely a protein that cross-reacts with anti-60-kDa Ro autoantibodies, as others have suggested.
Assuntos
Autoantígenos/metabolismo , Queratinócitos/imunologia , RNA Citoplasmático Pequeno , Ribonucleoproteínas/metabolismo , Animais , Anticorpos/imunologia , Autoanticorpos/imunologia , Autoantígenos/química , Autoantígenos/imunologia , Células Cultivadas , Reações Cruzadas , Epiderme/imunologia , Humanos , Peso Molecular , Coelhos , Proteínas Recombinantes , Ribonucleoproteínas/química , Ribonucleoproteínas/imunologia , Distribuição TecidualRESUMO
OBJECTIVE: To survey genitourinary physicians in the United Kingdom on their approaches to the management of vulvar lichen sclerosus. STUDY DESIGN: Questionnaire survey of United Kingdom genitourinary consultants. RESULTS: Seventy-one percent of genitourinary physicians biopsy vulvar lichen sclerosus. For treatment, 78% of clinicians use a high-grade topical corticosteroid, such as 0.05% clobetasol propionate. Topical sex steroids are used by a minority of clinicians. Cases are followed by 80% of respondents. Having made the diagnosis of this condition, 30% of respondents refer the case to either a dermatologist or gynecologist for further management. Fifty-two percent refer only after treatment failure. CONCLUSION: Genitourinary physicians in the United Kingdom see and manage cases of vulvar lichen sclerosus in genitourinary medicine clinics. However, a majority of clinicians refer these cases at some stage. A coordinated and multispecialty approach between genitourinary physicians, dermatologists and gynecologists would provide coherent management of vulvar lichen sclerosus.
Assuntos
Assistência Ambulatorial/métodos , Líquen Escleroso e Atrófico/tratamento farmacológico , Doenças da Vulva/tratamento farmacológico , Administração Tópica , Anti-Inflamatórios/uso terapêutico , Estrogênios/uso terapêutico , Feminino , Glucocorticoides , Humanos , Líquen Escleroso e Atrófico/patologia , Padrões de Prática Médica/estatística & dados numéricos , Encaminhamento e Consulta , Inquéritos e Questionários , Testosterona/uso terapêutico , Reino Unido , Doenças da Vulva/patologiaAssuntos
Ciclosporina/uso terapêutico , Doença de Darier/diagnóstico , Fármacos Dermatológicos/uso terapêutico , Doenças da Vulva/diagnóstico , Adulto , Ciclosporina/administração & dosagem , Doença de Darier/tratamento farmacológico , Doença de Darier/patologia , Fármacos Dermatológicos/administração & dosagem , Diagnóstico Diferencial , Feminino , Humanos , Doenças da Vulva/tratamento farmacológico , Doenças da Vulva/patologiaRESUMO
The behaviour of cutaneous disease in systemic lupus erythematosus (SLE) and discoid lupus erythematosus (DLE), under the influence of various hormonal states, was studied in 68 patients. In 28 pregnancies, cutaneous disease was essentially unchanged. In a total of 57 patients whose lupus erythematosus (LE) had been diagnosed prior to the menopause, 20% described a premenstrual cutaneous exacerbation. Only three patients had taken an oestrogen-containing contraceptive. The duration of oral contraceptive treatment before the onset of lupus varied: 1 month in a patient presenting with the acute malar rash of SLE, 2 months in a patient who presented with annular weals and later developed systemic features, and 12 months in a patient who developed generalized DLE. Thirty-three patients were menopausal at the time of the study; 4% had noticed a perimenopausal cutaneous flare. There was no deterioration in the skin of the five patients on hormone replacement therapy.
Assuntos
Hormônios Esteroides Gonadais/metabolismo , Lúpus Eritematoso Cutâneo/patologia , Pele/patologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Anticoncepcionais Orais Hormonais/metabolismo , Estrogênios/metabolismo , Feminino , Humanos , Lúpus Eritematoso Cutâneo/metabolismo , Menopausa/metabolismo , Menstruação/metabolismo , Pessoa de Meia-Idade , Gravidez , Complicações na Gravidez/metabolismo , Complicações na Gravidez/patologiaRESUMO
The human papilloma virus is implicated in causing several diseases, ranging from the common wart to malignancy. We describe a high prevalence of cutaneous warts in lupus erythematosus. The presence of warts did not correlate with the taking of immunosuppressive drugs. This observation suggests that there is a primary immunological defect among patients with lupus erythematosus. We found this high rate among patients with discoid as well as systemic lupus erythematosus. We found no correlation between the prevalence of cutaneous warts and cervical dysplasia, or malignancy. Discoid lupus erythematosus is often considered to be a different disease from systemic lupus erythematosus, running a more benign course. The high prevalence of cutaneous warts in both conditions highlights yet another similarity between these two diseases.
Assuntos
Lúpus Eritematoso Discoide/complicações , Lúpus Eritematoso Sistêmico/complicações , Verrugas/complicações , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Feminino , Humanos , Lúpus Eritematoso Discoide/imunologia , Lúpus Eritematoso Sistêmico/imunologia , Masculino , Pessoa de Meia-Idade , Papillomaviridae , Infecções Tumorais por Vírus/complicações , Displasia do Colo do Útero/complicaçõesRESUMO
We treated seven patients with discoid lupus erythematosus (DLE) with Vitamin E in an oral dose of 400 mg three times per day for 12 weeks. All other systemic and topical treatments were discontinued 1 month before initiation of the trial. The drug was then stopped and follow-up continued for at least another 4 weeks. No patient showed clearing of lesions. The trial was conducted during summer, when DLE is likely to be most active. There was no deterioration in any patient. No side effects were noted.
Assuntos
Lúpus Eritematoso Discoide/tratamento farmacológico , Vitamina E/uso terapêutico , Administração Oral , Adulto , Idoso , Relação Dose-Resposta a Droga , Feminino , Seguimentos , Humanos , Lúpus Eritematoso Discoide/epidemiologia , Lúpus Eritematoso Discoide/fisiopatologia , Masculino , Pessoa de Meia-Idade , Projetos Piloto , Índice de Gravidade de Doença , Fatores de Tempo , Vitamina E/administração & dosagem , Vitamina E/efeitos adversosRESUMO
Vulval splitting may be seen in association with clinically apparent dermatological disease but occasionally occurs in patients presenting with dyspareunia only. These patients usually have normal-looking vulvas on examination, apart from splitting. They are usually diagnosed as vulval vestibulitis. We have biopsied several patients with isolated vulval splitting with surprising results. Between the months of October 2000 and April 2002, 310 new patients were seen in the vulval clinic at Hope Hospital, which is a tertiary referral centre. Among these were nine cases of isolated vulval splitting, eight of which had histological features of dermatological disease on vulval biopsies. There were three cases of lichen planus, three with chronic dermatitis and two with candidiasis.
Assuntos
Dermatopatias/complicações , Doenças da Vulva/etiologia , Adulto , Idoso , Biópsia , Candidíase/complicações , Dermatite/complicações , Feminino , Humanos , Líquen Plano/complicações , Pessoa de Meia-Idade , Dermatopatias/patologia , Vulva/patologia , Doenças da Vulva/patologiaRESUMO
Incontinentia pigmenti (Bloch-Sulzberger syndrome) is an uncommon genodermatosis mainly affecting females. It consists of characteristic skin manifestations associated in 79.8% of patients with other systemic manifestations. Of these there have been five cases of palatal or lip abnormalities, none being bilateral cleft lip and palate. We describe a unique case of incontinentia pigmenti associated with a bilateral cleft lip and palate.
Assuntos
Fenda Labial/complicações , Fissura Palatina/complicações , Incontinência Pigmentar/complicações , Fenda Labial/patologia , Fissura Palatina/patologia , Feminino , Humanos , Incontinência Pigmentar/patologia , Recém-Nascido , Perna (Membro) , Boca/patologia , Pele/patologiaRESUMO
Acantholysis is a feature of disorders such as Hailey-Hailey disease and Darier's disease. Immunocytochemical studies have shown internalization of desmosomal components after acantholysis. Basal cytokeratins show suprabasal expression in lesional Darier's disease. The exact mechanisms of acantholysis are still unclear. Cantharidin induces blistering, with suprabasal keratinocyte acantholysis, possibly by protease activation. Plasmin has been implicated in the pathogenesis of acantholysis in Darier's disease and Hailey-Hailey disease. We examined the distribution of desmosomal components, proteases and cytokeratins in cantharidin blisters, to compare them with those previously found in Darier's disease and Hailey-Hailey disease. Two drops of cantharidin collodion were applied to the skin of five normal volunteers. A 4-mm punch biopsy of the blister was taken, and snap frozen. Sections were stained with antibodies to desmosomal proteins (dp) 1/2, dp 3, desmosomal glycoproteins (dg) 1, 2/3, extracellular carbohydrate residues, using the lectins peanut agglutinin (PNA) and soybean agglutinin (SBA), proteases and cytokeratins. Acantholytic cells were stained diffusely with dp1/2; there was markedly reduced or absent peripheral staining for dp3, dg1, dg2/3, PNA and SBA. There was no clumping of stain. Plasminogen, fibrinogen and urokinase were expressed in some acantholytic cells. Basal keratin markers were expressed suprabasally in acantholytic cells. These results are similar to those previously obtained in Darier's disease, but different from the staining obtained in Hailey-Hailey disease. Extracellular glycosylated portions of adhesion molecules may be lost after acantholysis, perhaps as a result of conformational changes, internalization of extracellular domains, or proteolysis. The changes in the expression of plasminogen, fibrinogen, urokinase and cytokeratins in acantholytic cells in cantharidin-induced blisters are, as in Darier's disease and Hailey-Hailey disease, probably secondary to acantholysis, and changes in the shape of cells. We conclude that cantharidin blisters may be a useful model for the study of acantholysis in Darier's disease.
Assuntos
Acantólise/metabolismo , Moléculas de Adesão Celular/metabolismo , Queratinas/metabolismo , Peptídeo Hidrolases/metabolismo , Acantólise/induzido quimicamente , Cantaridina , Proteínas do Citoesqueleto/metabolismo , Desmoplaquinas , Fibrinogênio/metabolismo , Humanos , Imuno-Histoquímica , Modelos Biológicos , Plasminogênio/metabolismo , Ativador de Plasminogênio Tecidual/metabolismo , Ativador de Plasminogênio Tipo Uroquinase/metabolismoRESUMO
We have examined the action of cantharidin on the skin of patients with Darier's disease, and used immunohistological techniques to determine the distribution of desmosomal components, keratin intermediate filaments, and proteases in cantharidin-induced blisters. Cantharidin induced acantholysis, but the presence of acantholysis did not trigger the development of the characteristic warty, dyskeratotic papules in patients with Darier's disease. The distribution of desmosomal components, keratins and proteases within the acantholytic keratinocytes in the cantharidin-induced blisters was similar to that previously found in acantholytic cells within lesions of Darier's disease: peripheral staining for extracellular desmosomal components was reduced; some desmosomal components were detected diffusely in the acantholytic cells; basal cell keratin markers were expressed by some suprabasal acantholytic cells, and plasminogen was detected in association with acantholytic cells. Cleavage of desmosomes did not reveal the underlying abnormality in Darier's disease.
Assuntos
Acantólise/induzido quimicamente , Cantaridina , Doença de Darier/patologia , Desmossomos/patologia , Pele/patologia , Acantólise/patologia , Adulto , Membrana Basal/patologia , Colágeno/análise , Proteínas do Citoesqueleto/análise , Desmoplaquinas , Feminino , Fibrinogênio/análise , Humanos , Imuno-Histoquímica , Queratinócitos/patologia , Queratinas/análise , Masculino , Pessoa de Meia-Idade , Plasminogênio/análise , Ativador de Plasminogênio Tipo Uroquinase/análiseRESUMO
We have assessed the cutaneous signs in 73 patients with systemic lupus erythematosus (SLE), seen during a 5-year period in an English hospital. Most previous information about the cutaneous manifestations of SLE has been obtained from studies performed in the U.S.A. We classified lesions as specific cutaneous and mucosal LE (acute, subacute and chronic) or non-specific LE-related, e.g. photosensitivity, urticaria, erythema, Raynaud's phenomenon or vasculitis. Acute cutaneous LE lesions included a butterfly rash with erythematous macules, telangiectasia or papulosquamous lesions, seen in 37 patients (51%) and facial oedema seen in four patients (5%). Five patients (7%) had psoriasiform subacute cutaneous LE. Chronic cutaneous LE was common: 18 patients (25%) had chronic discoid lesions (DLE) and, in 12 (15%), these had preceded systemic disease. One patient had facial lupus profundus. Ten patients (14%) had scarring alopecia secondary to DLE. Fifteen patients (20.5%) had chronic chilblain lupus. Twenty-three patients (31.5%) had a history of mouth ulceration. Of these, 11 (15%) gave a history of ulcers at the onset of their disease. Three (4%) had erythema and superficial ulceration of the palate, not typical of aphthous ulcers, and three (4%) had chronic buccal plaques. Cheilitis due to DLE was seen in three (4%), episcleritis in three (4%), five (7%) had nasal disease, six (8%) bullous skin eruptions, one 'the bullous eruption of SLE', four bullae associated with cutaneous vasculitis, and one bullae associated with ultraviolet radiation. Forty-six (63%) observed photosensitivity. A non-scarring alopecia occurred in 29 (40%). Vascular phenomena were common: three patients (4%) had chronic palmar erythema, Raynaud's phenomenon occurred in 44 patients (60%), chronic urticaria, worsened by sun exposure, was noted by 32 (44%) (in whom the lesions often lasted more than 36 h), eight (11%) had cutaneous vasculitis and three (4%) livedo reticularis. Skin changes play a prominent part in SLE and may provide helpful diagnostic information. In this British population, chilblains and urticaria were particularly common. Lesions of subacute cutaneous LE were relatively unusual in this group of patients with SLE.
Assuntos
Lúpus Eritematoso Sistêmico/complicações , Dermatopatias/etiologia , Adolescente , Adulto , Idoso , Alopecia/etiologia , Criança , Feminino , Humanos , Lúpus Eritematoso Cutâneo/patologia , Lúpus Eritematoso Discoide/patologia , Masculino , Pessoa de Meia-Idade , Doenças da Boca/etiologia , Doenças da Boca/patologia , Transtornos de Fotossensibilidade/etiologia , Dermatopatias/patologia , Dermatopatias Vasculares/etiologia , Úlcera/etiologia , Úlcera/patologiaRESUMO
A 79-year-old virgo intacta presented with a 20-year-history of intertrigo, and a 3-month history of superimposed warty masses beneath both breasts and in the groin and perianal areas. There was no evidence of immunosuppression. Histology of the warty lesions showed squamous papillomata, with evidence of wart virus infection. Human papillomavirus (HPV) type 6 was identified by in situ DNA hybridization, in the submammary lesions. This is an unusual manifestation of both intertrigo and wart virus infection. HPV-6 is classically found in anogenital warts. We assume that these warts were acquired by a non-venereal route and/or by congenital infection some 78 years ago. We suggest that it is the warm, moist environment, rather than the specific site, which encourages HPV-6 to flourish.
Assuntos
Condiloma Acuminado/complicações , Intertrigo/complicações , Neoplasias Cutâneas/complicações , Idoso , Canal Anal , Mama , Condiloma Acuminado/patologia , Feminino , Virilha , Humanos , Intertrigo/patologia , Papillomaviridae , Pele/patologia , Neoplasias Cutâneas/patologiaRESUMO
We describe a patient with the rare diagnosis of 'Bullous Systemic Lupus Erythematosus'. She is unusual in presenting with classical dermatitis herpetiformis, before the emergence of systemic features. In addition, on indirect immunofluorescence on sodium chloride split skin, there was epidermal binding. Immunoblotting was negative for type VII collagen.