Suburban groundwater quality as influenced by turfgrass and septic sources, delmarva peninsula, USA.

Suburban land use is expanding in many parts of the United States and there is a need to better understand the potential water-quality impacts of this change. This study characterized groundwater quality in a sandy, water-table aquifer influenced by suburban development and compared the results to known patterns in water chemistry associated with natural, background conditions and agricultural effects. Samples for nutrients, major ions, and isotopes of N and O in NO were collected in 2011 beneath turfgrass from 29 shallow wells (median depth 3.7 m) and from 18 deeper wells (median depth 16.9 m) in a long-term suburban development. Nitrate (as N) concentrations in groundwater beneath turfgrass were highly variable (0.02-22.3 mg L) with a median of 2.7 mg L, which is higher than natural water chemistry (>0.4 mg L; Na-Cl-HCO water type), but significantly lower than concentrations beneath a nearby agricultural area (median 16.9 mg L; < .0001). Dissolved Fe concentrations in shallow suburban groundwater, attributed to chelated Fe in turfgrass fertilizers, were significantly higher ( < .005) than concentrations from the agricultural site, although a Ca-Mg-Cl-NO water type was dominant in both areas. A Na-Cl-NO water type indicated a septic-system source for nitrate in deep suburban groundwater (0.06-6.0 mg L; median 1.5 mg L). Isotopic data indicated denitrification; however, geochemical techniques were more helpful in identifying nitrate sources. Results indicate that suburban expansion into agricultural areas may significantly decrease overall nitrate concentrations in groundwater, but excessive turfgrass fertilization could result in localized contamination.

Effects of nitrate and ammonium on assimilation of nitric oxide by Heterosigma akashiwo.

The harmful alga Heterosigma akashiwo possesses a hybrid nitrate reductase (NR) enzyme, NR2-2/2HbN, which has the potential to convert NO to nitrate for assimilation into biomass. In previous research, NR transcription in H. akashiwo was induced by nitrate while NR activity was inhibited by ammonium. Here, the capacity of H. akashiwo to use NO in the presence of nitrate and/or ammonium was investigated to understand the regulation of NO assimilation. Continuous cultures of H. akashiwo were acclimated to growth on nitrate, ammonium, or a mixture of both. Aliquots from these cultures were spiked with 15N-labeled NO. The expression of genes involved in nitrogen assimilation was evaluated, as well as nitrate reductase activity and assimilation of 15N-labeled nitrogen into algal biomass. Results showed that NO induced expression and activity of NR, and upregulated expression of GOGAT regardless of the presence of other inorganic nitrogen sources, while GS expression decreased over time. Furthermore, 15NO uptake and assimilation was significantly higher in cultures acclimated for growth on ammonium compared to cultures acclimated for growth on nitrate alone. Assimilation of NO may provide H. akashiwo with a competitive advantage in N-poor environments or areas with elevated NO.

Nitrogen loads to estuaries from waste water plumes: modeling and isotopic approaches.

We developed, and applied in two sites, novel methods to measure ground water-borne nitrogen loads to receiving estuaries from plumes resulting from land disposal of waste water treatment plant (WWTP) effluent. In addition, we quantified nitrogen losses from WWTP effluent during transport through watersheds. WWTP load to receiving water was estimated as the difference between total measured ground water-transported nitrogen load and modeled load from major nitrogen sources other than the WWTP. To test estimated WWTP loads, we applied two additional methods. First, we quantified total annual waste water nitrogen load from watersheds based on nitrogen stable isotopic signatures of primary producers in receiving water. Second, we used published data on ground water nitrogen concentrations in an array of wells to estimate dimensions of the plume and quantify the annual mass of nitrogen transported within the plume. Loss of nitrogen during transport through the watershed was estimated as the difference between the annual mass of nitrogen applied to watersheds as treatment plant effluent and the estimated nitrogen load reaching receiving water. In one plume, we corroborated our estimated nitrogen loss in watersheds using data from multiple-level sampling wells to calculate the loss of nitrogen relative to a conservative tracer. The results suggest that nitrogen from the plumes is discharging to the estuaries but that substantial nitrogen loss occurs during transport through the watersheds. The measured vs. modeled and stable isotopic approaches, in comparison to the plume mapping approach, may more reliably quantify ground water-transported WWTP loads to estuaries.