RESUMO
Porcine pancreatic lipase catalyzes the transesterification reaction between tributyrin and various primary and secondary alcohols in a 99 percent organic medium. Upon further dehydration, the enzyme becomes extremely thermostable. Not only can the dry lipase withstand heating at 100 degrees C for many hours, but it exhibits a high catalytic activity at that temperature. Reduction in water content also alters the substrate specificity of the lipase: in contrast to its wet counterpart, the dry enzyme does not react with bulky tertiary alcohols.
Assuntos
Enzimas/metabolismo , Temperatura Alta , Álcoois/metabolismo , Animais , Catálise , Lipase/metabolismo , Pâncreas/enzimologia , Soluções , Suínos , Triglicerídeos/metabolismo , Água/metabolismoRESUMO
The number of industrial processes for the synthesis of fine and commodity chemicals, pharmaceutical and agrochemical intermediates and drug substances utilizing biological catalysts continues to grow. The combination of new molecular biology techniques, such as directed evolution and pathway engineering, with new and efficient high-throughput screening methods is poised to bolster this field and further advance the contribution of biocatalysis to the chemical and the pharmaceutical industries.
Assuntos
Indústria Química/métodos , Indústria Farmacêutica/métodos , Enzimas/metabolismo , Preparações Farmacêuticas/síntese química , Catálise , Indústria Química/tendências , Evolução Molecular Direcionada/métodos , Indústria Farmacêutica/tendências , Enzimas/química , Engenharia Genética/métodos , Herbicidas/síntese química , Hidroliases/química , Hidroliases/metabolismo , Penicilina Amidase/química , Penicilina Amidase/metabolismoRESUMO
A glucuronide of a novel cholesterol absorption inhibitor was synthesized on a 200-mg scale in one step via bovine liver glucuronyltransferase-catalyzed coupling of the glucuronyl moiety of UDP-glucuronic acid with the phenolic hydroxyl of Sch 58235. It was shown that the product yield is limited by the hydrolysis of UDP-glucuronic acid by impurities present in the commercial microsomal preparation of the transferase. This detrimental effect of UDPGluA hydrolysis could be diminished by the presence of high concentration of glucuronlytransferase. Optimization of reaction conditions and purification procedure resulted in a process that proceeded with 95% conversion and 88% isolated product yield. The 13C6-glucuronide of Sch 58235 was prepared with the help of a cascade of eight enzymes operating concurrently in one pot.
Assuntos
Anticolesterolemiantes/metabolismo , Azetidinas/metabolismo , Glucuronosiltransferase/metabolismo , Animais , Anticolesterolemiantes/química , Azetidinas/química , Catálise , Bovinos , Ezetimiba , Glucuronatos/síntese química , Concentração de Íons de Hidrogênio , Microssomos Hepáticos/enzimologiaRESUMO
Specific autoantibodies of neutralizing character to histamine, serotonin, kallikrein, bradykuinine, acetylcholine, adrenalin, noradrenalin, insulin, and to the serotonin metabolite--5-hydroxyindolacetic acid, were found in donor serum (47.2%) and in intact rabbits, guinea pigs, rats, and mice (24.4%). Anti0odies to histamine, serotonin, kallikrein were found in a number of samples of commercial gamma-globulin. They were relatively often observed during the morning and day-time hours, in summer and in winter, in luteal phase of menstrual cycle, early in pregnancy (6-8 weeks), after surgical treatment, burns, inflammation, administration of foreign albumin, in the course of immunization by different antigens. The results of fractional analysis of serum showed that antibodies belonged to immunoglobulins with the constant of sedimantation 7S and 19S. The data obtained suggest the existence of an immune mechanism for the content regulation of different substances of endogenous origination in any organism.
Assuntos
Autoanticorpos , Acetilcolina/imunologia , Animais , Formação de Anticorpos , Autoanticorpos/análise , Bradicinina/imunologia , Epinefrina/imunologia , Feminino , Histamina/imunologia , Humanos , Ácido Hidroxi-Indolacético/imunologia , Soros Imunes/farmacologia , Imunização , Anticorpos Anti-Insulina/análise , Calicreínas/imunologia , Menstruação , Norepinefrina/imunologia , Placenta , Gravidez , Coelhos/imunologia , Ratos , Estações do Ano , Serotonina/imunologia , gama-Globulinas/análiseRESUMO
Experiments on rats and on human lymphocytes have indicated that a prompt immune response to low-molecular-weight biologically active agents is thymus-dependent, calls for cooperative interaction of T and B lymphocytes and macrophages, it is enhanced by T mitogens and implemented through the specific pharmacological receptors of T and B lymphocytes. The findings offer ample scope for controlling the immune mechanisms of habituation to drugs and poisons.
Assuntos
Hipersensibilidade a Drogas/etiologia , Hipersensibilidade Imediata/induzido quimicamente , Macrófagos/efeitos dos fármacos , Linfócitos T/efeitos dos fármacos , Animais , Formação de Anticorpos/efeitos dos fármacos , Células Cultivadas , Hipersensibilidade a Drogas/imunologia , Humanos , Hipersensibilidade Imediata/imunologia , Imunidade Celular/efeitos dos fármacos , Cooperação Linfocítica/efeitos dos fármacos , Cooperação Linfocítica/imunologia , Macrófagos/imunologia , Masculino , Peso Molecular , Ratos , Formação de Roseta , Linfócitos T/imunologia , TimectomiaAssuntos
Analgésicos/uso terapêutico , Azocinas/uso terapêutico , Antagonistas de Entorpecentes/uso terapêutico , Transtornos Relacionados ao Uso de Substâncias/tratamento farmacológico , Adolescente , Adulto , Idoso , Analgésicos/efeitos adversos , Azocinas/efeitos adversos , Ensaios Clínicos como Assunto , Ciclazocina/administração & dosagem , Ciclazocina/efeitos adversos , Ciclazocina/uso terapêutico , Eletroencefalografia , Heroína/antagonistas & inibidores , Humanos , Injeções Intravenosas , Relações Interpessoais , Masculino , Pessoa de Meia-Idade , Antagonistas de Entorpecentes/efeitos adversos , Comportamento Sexual , Transtornos Relacionados ao Uso de Substâncias/reabilitaçãoAssuntos
Bradicinina/imunologia , Inflamação/metabolismo , Calicreínas/imunologia , Animais , Anticorpos , Coelhos , RatosRESUMO
Subtilisin and alpha-chymotrypsin vigorously act as catalysts in a variety of dry organic solvents. Enzymatic transesterifications in organic solvents follow Michaelis-Menten kinetics, and the values of V/Km roughly correlate with solvent's hydrophobicity. The amount of water required by chymotrypsin and subtilisin for catalysis in organic solvents is much less than needed to form a monolayer on its surface. The vastly different catalytic activities of chymotrypsin in various organic solvents are partly due to stripping of the essential water from the enzyme by more hydrophilic solvents and partly due to the solvent directly affecting the enzymatic process. The rate enhancements afforded by chymotrypsin and subtilisin in the transesterification reaction in octane are of the order of 100 billion-fold; covalent modification of the active center of the enzymes by a site-specific reagent renders them catalytically inactive in organic solvents. Upon replacement of water with octane as the reaction medium, the specificity of chymotrypsin toward competitive inhibitors reverses. Both thermal and storage stabilities of chymotrypsin are greatly enhanced in nonaqueous solvents compared to water. The phenomenon of enzymatic catalysis in organic solvents appears to be due to the structural rigidity of proteins in organic solvents resulting in high kinetic barriers that prevent the native-like conformation from unfolding.
Assuntos
Catálise , Quimotripsina/metabolismo , Solventes , Subtilisinas/metabolismo , Quimotripsina/antagonistas & inibidores , Estabilidade de Medicamentos , Esterificação , Liofilização , Temperatura Alta , Concentração de Íons de Hidrogênio , Cinética , Octanos , Conformação Proteica , Termodinâmica , ÁguaRESUMO
Three model, unrelated enzymes (yeast alcohol oxidase, mushroom polyphenol oxidase, and horse liver alcohol dehydrogenase) were found to be catalytically active in a variety of organic solvents. For all enzymes and solvents tested, the enzymatic activity greatly increased upon an increase in the water content in the solvents (which always remained below the solubility limit). Much less water was required to reach the maximal activity in hydrophobic solvents than in their hydrophilic counterparts. However, when the catalytic activity was plotted versus the amount of water bound to the enzymes, a common pattern emerged for different solvents. These data suggest that the effect of organic solvents on an enzyme is primarily due to interactions with the enzyme-bound, essential layer of water rather than with the enzyme itself. At optimal water contents, enzymatic activities in organic solvents were in the range from 20 to 40% of those in aqueous solutions. From experiments on (i) replacement of water with other hydrogen bond-forming additives and (ii) titration of enzyme amino groups in an organic medium, as well as the literature data on dehydrated enzymes, it is concluded that the water required by enzymes in nonaqueous solvents provides them with sufficient conformational flexibility needed for catalysis.
Assuntos
Álcool Desidrogenase , Oxirredutases do Álcool , Catecol Oxidase , Modelos Químicos , Água , 1-Butanol , 1-Octanol , Acetatos , Animais , Basidiomycota/enzimologia , Butanóis , Cavalos , Octanóis , Saccharomyces cerevisiae/enzimologia , SolventesRESUMO
In the study of obtained data on the part of immune mechanism in maintaining chemical homeostasis the authors found, by means of in vitro experiments, certain properties characterizing participation of the lymphatic apparatus of various rabbit and rat organs in the synthesis of antibodies against mediators, their metabolites, enzymes and hormons in comparison with effect of stimulators, [fytohemagglutinin, staphylococcal filtrate, xenogenic mixed lymphocytic cultures], active and passive immunization of potential cell preparations for the production of specific autoantibodies. Formation of the mechanism is shown on ontogenesis. It was ascertained that pathologic processes [adjuvant arthritis, rejection reaction, immobilization stress, sepsis] essentially act upon conditions of the immune mechanism of chemical homeostasis.
Assuntos
Formação de Anticorpos , Tecido Linfoide/imunologia , Animais , Especificidade de Anticorpos , Artrite Experimental/imunologia , Células Cultivadas , Humanos , Imunização , Imunização Passiva , Tecido Linfoide/embriologia , Fito-Hemaglutininas/farmacologia , Coelhos , Ratos , Infecções Estafilocócicas/imunologiaRESUMO
Insulin-like growth factor-I (IGF-I) is a polypeptide mitogen which is regulated by growth hormone (GH). IGF-I mediates many of the biological functions of GH, including the maintenance of lymphoid mass and functions. Since GH secretion declines with age, we asked whether changes in the availability of IGF-I might contribute to age-associated alterations in immune functions. As a first step, we examined relationships between plasma levels of IGF-I and in vitro correlates of immunity in young and elderly subjects. Heparinized plasma and lymphocytes were collected from the peripheral blood of 34 healthy young (aged 27 +/- 0.9 years, mean +/- SEM) and 41 elderly (79 +/- 1.3 years) volunteers (31 males and 44 females in total). Plasma levels of IGF-I, measured by radioimmunoassay after the removal of IGF-I-binding proteins, were reduced among elders compared to young controls (138 +/- 8.7 ng/mL vs 80.2 +/- 4.7 ng/mL, P < 0.001). The number of circulating lymphocytes did not change with age. The proliferative response ([3H]thymidine uptake into DNA) of T-cells to concanavalin A and B-cells to pokeweed mitogen were reduced among elders (P < 0.05). An increased spontaneous antitumor natural killer (NK) activity (P < 0.001) was accompanied by a higher percentage of CD16(+) NK cells among lymphocytes in older subjects (P < 0.001). The NK cell number was positively related to IGF-I levels in young volunteers but not among elders. Correlation analysis demonstrated a highly significant relationship between plasma IGF-I levels and T-cell (but not B-cell) proliferative response during aging (r = 0.492, P < 0.001). Our results imply that reduced immunocompetence may be one of the consequences of reduced IGF-I levels in human aging. Among the three types of immune cells tested, the T-cells were most sensitive to fluctuations in IGF-I levels. Reduced IGF-I availability may be one of the determinants of the decline in T-cell-mediated immune function in the elderly. To our knowledge, this is the first report presenting correlative data on concurrent changes in IGF-I levels and immune parameters in human aging.