Catheter-directed thrombolysis versus systemic anticoagulation in the treatment of symptomatic splanchnic venous thrombosis secondary to acute pancreatitis: a retrospective cohort study.

BACKGROUND: Catheter-directed thrombolysis (CDT) has been an important therapy and seems effective in patients with splanchnic venous thrombosis (SVT) secondary to some diseases, but this intervention hasn't been formally evaluated in the setting of acute pancreatitis (AP). METHODS: This was a retrospective study enrolled patients between January 2013 and December 2018. AP patients who developed SVT-induced symptoms, including intractable ascites and/or enteral nutrition intolerance, were included. Demographics, SVT associated parameters, clinical features and outcomes, long-term quality of life evaluated by using SF-36 questionnaire were compared between CDT group and systemic anticoagulation (SAC) group. RESULTS: 6 patients underwent CDT and 17 received SAC. Patients in CDT group had a higher recanalization rate (100% versus 35.3%; p = 0.014) and shorter time to symptoms resolution (median 8 days versus. 31.5 days, p = 0.004). Mortality and length of hospital stay were comparable between two groups. The association analysis indicated that CDT use exerted a significantly beneficial effect on recanalization rate (risk ratio, 2.833; 95% CI, 1.489 to 5.393; p = 0.002) and time to symptoms resolution (mean difference, -33.333; 95% CI, -64.612 to -2.055; p = 0.038). No SVT-related symptoms recurrence was recorded in survivors at six-month follow-up. There was no statistical difference in either item of SF-36 questionnaire between two groups. CONCLUSIONS: Compared with SAC, CDT may facilitate vascular recanalization and shorten symptom resolution for symptomatic SVT.

Comparison of buckwheat genomes reveals the genetic basis of metabolomic divergence and ecotype differentiation.

Golden buckwheat (Fagopyrum dibotrys or Fagopyrum cymosum) and Tartary buckwheat (Fagopyrum tataricum) belong to the Polygonaceae and the Fagopyrum genus is rich in flavonoids. Golden buckwheat is a wild relative of Tartary buckwheat, yet golden buckwheat is a traditional Chinese herbal medicine and Tartary buckwheat is a food crop. The genetic basis of adaptive divergence between these two buckwheats is poorly understood. Here, we assembled a high-quality chromosome-level genome of golden buckwheat and found a one-to-one syntenic relationship with the chromosomes of Tartary buckwheat. Two large inversions were identified that differentiate golden buckwheat and Tartary buckwheat. Metabolomic and genetic comparisons of golden buckwheat and Tartary buckwheat indicate an amplified copy number of FdCHI, FdF3H, FdDFR, and FdLAR gene families in golden buckwheat, and a parallel increase in medicinal flavonoid content. Resequencing of 34 wild golden buckwheat accessions across the two morphologically distinct ecotypes identified candidate genes, including FdMYB44 and FdCRF4, putatively involved in flavonoid accumulation and differentiation of plant architecture, respectively. Our comparative genomic study provides abundant genomic resources of genomic divergent variation to improve buckwheat with excellent nutritional and medicinal value.

Template Thermolysis to Create a Carbon Dots-Embedded Mesoporous Titanium-Oxo Sulfate Framework for Visible-Light Photocatalytic Applications.

An organo-templated titanium-oxo sulfate of the formulation (H2nep)[TiO(SO4)2] (denoted as TiOS, nep = N-ethylpiperazine) was synthesized under solvent-free conditions. The framework of TiOS is assembled from the [TiO(SO4)2]n2n- infinite chains interconnected by the H2nep cations through H-bond networks. After thermal treatment under vacuum conditions, the organic template H2nep was partially decomposed and converted into N-doped carbon dots (N-CDs), resulting in the N-CDs@TiOS composite material with retained crystallinity of the parent TiOS. The thermolysis of organic templates generates meso-cavities in the framework, rendering N-CDs@TiOS with a mesoporous structure. Photoelectrochemical and photocatalytic experiments show that the presence of N-CDs substantially improved visible-light-driven photocatalytic activity of N-CDs@TiOS compared to that of TiOS. The template thermolysis strategy gives an effective approach to construct the CDs-sensitized Ti-based mesoporous open-framework materials for visible-light photocatalytic applications.

Genome-wide association for grain morphology in synthetic hexaploid wheats using digital imaging analysis.

BACKGROUND: Grain size and shape greatly influence grain weight which ultimately enhances grain yield in wheat. Digital imaging (DI) based phenomic characterization can capture the three dimensional variation in grain size and shape than has hitherto been possible. In this study, we report the results from using digital imaging of grain size and shape to understand the relationship among different components of this trait, their contribution to enhance grain weight, and to identify genomic regions (QTLs) controlling grain morphology using genome wide association mapping with high density diversity array technology (DArT) and allele-specific markers. RESULTS: Significant positive correlations were observed between grain weight and grain size measurements such as grain length (r = 0.43), width, thickness (r = 0.64) and factor from density (FFD) (r = 0.69). A total of 231 synthetic hexaploid wheats (SHWs) were grouped into five different sub-clusters by Bayesian structure analysis using unlinked DArT markers. Linkage disequilibrium (LD) decay was observed among DArT loci > 10 cM distance and approximately 28% marker pairs were in significant LD. In total, 197 loci over 60 chromosomal regions and 79 loci over 31 chromosomal regions were associated with grain morphology by genome wide analysis using general linear model (GLM) and mixed linear model (MLM) approaches, respectively. They were mainly distributed on homoeologous group 2, 3, 6 and 7 chromosomes. Twenty eight marker-trait associations (MTAs) on the D genome chromosomes 2D, 3D and 6D may carry novel alleles with potential to enhance grain weight due to the use of untapped wild accessions of Aegilops tauschii. Statistical simulations showed that favorable alleles for thousand kernel weight (TKW), grain length, width and thickness have additive genetic effects. Allelic variations for known genes controlling grain size and weight, viz. TaCwi-2A, TaSus-2B, TaCKX6-3D and TaGw2-6A, were also associated with TKW, grain width and thickness. In silico functional analysis predicted a range of biological functions for 32 DArT loci and receptor like kinase, known to affect plant development, appeared to be common protein family encoded by several loci responsible for grain size and shape. CONCLUSION: Conclusively, we demonstrated the application and integration of multiple approaches including high throughput phenotyping using DI, genome wide association studies (GWAS) and in silico functional analysis of candidate loci to analyze target traits, and identify candidate genomic regions underlying these traits. These approaches provided great opportunity to understand the breeding value of SHWs for improving grain weight and enhanced our deep understanding on molecular genetics of grain weight in wheat.

Poria cocos-derived carbon dots for parallel detection of Cr6+/Fe3+ in complex environments with superior sensitivity.

Multifunctional sensor capable of parallel sensing is of great importance thanks to their wide applications and great practicality. In this report, Poria cocos-derived carbon dots (CDs) were adopted for the development of multifunctional sensor for the parallel detection of Cr6+ and Fe3+ with superior sensitivity and applicability. Specifically, extremely low limit of detection (LOD) of 1.07 × 10-3 nM and 1.98 × 10-3 nM were achieved for Cr6+ and Fe3+, respectively. Systematic mechanism explorations revealed that the highly sensitive detection of Cr6+ was attributed to an efficient inner filter effect (IFE), while the sensing of Fe3+ was realized due to a strong static quenching process. Furthermore, the assay was found to be extremely versatile, achieving the reliable detection of Cr6+ and Fe3+ in multiple natural water environments and even biological environment. Utilizing the different reactions of Cr6+ and Fe3+ towards masking reagents, a logic gate that could effectively eliminate the mutual interference of Cr6+ and Fe3+ was successfully designed.

Battery-free, wireless, and electricity-driven soft swimmer for water quality and virus monitoring.

Miniaturized mobile electronic system is an effective candidate for in situ exploration of confined spaces. However, realizing such system still faces challenges in powering issue, untethered mobility, wireless data acquisition, sensing versatility, and integration in small scales. Here, we report a battery-free, wireless, and miniaturized soft electromagnetic swimmer (SES) electronic system that achieves multiple monitoring capability in confined water environments. Through radio frequency powering, the battery-free SES system demonstrates untethered motions in confined spaces with considerable moving speed under resonance. This system adopts soft electronic technologies to integrate thin multifunctional bio/chemical sensors and wireless data acquisition module, and performs real-time water quality and virus contamination detection with demonstrated promising limits of detection and high sensitivity. All sensing data are transmitted synchronously and displayed on a smartphone graphical user interface via near-field communication. Overall, this wireless smart system demonstrates broad potential for confined space exploration, ranging from pathogen detection to pollution investigation.

A soft implantable energy supply system that integrates wireless charging and biodegradable Zn-ion hybrid supercapacitors.

The advent of implantable bioelectronic devices offers prospective solutions toward health monitoring and disease diagnosis and treatments. However, advances in power modules have lagged far behind the tissue-integrated sensor nodes and circuit units. Here, we report a soft implantable power system that monolithically integrates wireless energy transmission and storage modules. The energy storage unit comprises biodegradable Zn-ion hybrid supercapacitors that use molybdenum sulfide (MoS2) nanosheets as cathode, ion-crosslinked alginate gel as electrolyte, and zinc foil as anode, achieving high capacitance (93.5 mF cm-2) and output voltage (1.3 V). Systematic investigations have been conducted to elucidate the charge storage mechanism of the supercapacitor and to assess the biodegradability and biocompatibility of the materials. Furthermore, the wirelessly transmitted energy can not only supply power directly to applications but also charge supercapacitors to ensure a constant, reliable power output. Its power supply capabilities have also been successfully demonstrated for controlled drug delivery.

Isolation and characterization of novel microsatellite markers for Avena sativa (Poaceae) (oat).

PREMISE OF THE STUDY: A new set of microsatellite primers was developed for Avena sativa and characterized to assess the level of genetic diversity among cultivars and wild genotypes. METHODS AND RESULTS: Using an enrichment genomic library, 14 simple sequence repeat markers were identified. The loci of these markers were characterized and found to be polymorphic in size among 48 genotypes of oat from diverse geographical locations. The number of alleles per locus ranged from two to eight, while the observed heterozygosity ranged from 0.031 to 0.75. CONCLUSIONS: These newly identified microsatellite markers will facilitate genetic diversity studies, fingerprinting, and genetic mapping of oat. Moreover, these new primers for A. sativa will aid future studies of polyploidy and hybridization in other species in this genus.

Six Underutilized Grain Crops for Food and Nutrition in China.

Underutilized grain crops are an essential part of the food system that supports humankind. A number of these crops can be found in China, such as barley, buckwheat, broomcorn millet, foxtail millet, oat, and sorghum, which have characteristics such as containing more nutritional elements, being resistant to biotic and abiotic stresses, and having strong adaptability to poor environments. The diversity of these crops provides options for farmers' livelihoods and healthy food for the population. Although some mentioned crops such as barley, oat, and sorghum are not underutilized crops globally, they could be considered underutilized in China as they were more important in the past and could be revitalized for food and nutrition in the future. This paper reviews current progress in research and development in the areas of germplasm resource conservation, variety improvement, cultivation technologies, processing, and the nutrition and benefits of six underutilized grain crops in China. It is concluded that underutilized grain crops could play a critical role in food and nutritional security in China.

3D morphable systems via deterministic microfolding for vibrational sensing, robotic implants, and reconfigurable telecommunication.

DNA and proteins fold in three dimensions (3D) to enable functions that sustain life. Emulation of such folding schemes for functional materials can unleash enormous potential in advancing a wide range of technologies, especially in robotics, medicine, and telecommunication. Here, we report a microfolding strategy that enables formation of 3D morphable microelectronic systems integrated with various functional materials, including monocrystalline silicon, metallic nanomembranes, and polymers. By predesigning folding hosts and configuring folding pathways, 3D microelectronic systems in freestanding forms can transform across various complex configurations with modulated functionalities. Nearly all transitional states of 3D microelectronic systems achieved via the microfolding assembly can be easily accessed and modulated in situ, offering functional versatility and adaptability. Advanced morphable microelectronic systems including a reconfigurable microantenna for customizable telecommunication, a 3D vibration sensor for hand-tremor monitoring, and a bloomable robot for cardiac mapping demonstrate broad utility of these assembly schemes to realize advanced functionalities.

Size modulation of MIL-125 nanocrystals to promote the catalytic performance towards oxidative desulfurization.

The Ti-based metal-organic framework (Ti-MOF) MIL-125 with tunable crystalline size in the range of ca. 50 nm to 1500 nm was synthesized by the coordination modulation method using trans-cinnamic acid (CA) as a modulator. The coordination modulation also induced hierarchical porosity and structure defects on the nanocrystals. A significant size-dependent catalytic activity towards the oxidative desulfurization (ODS) reaction was observed for these MIL-125 nanocrystals. In particular, the MIL-125 nanocrystals with a mean size of ca. 50 nm exhibit dramatically enhanced catalytic performance for the bulky sulfur compound 4,6-dimethyldibenzothiophene (4,6-DMDBT) compared to the microcrystals. It is demonstrated that the size modulation of MIL-125 is an effective approach to promote its performance for the catalysis of bulky molecules.

Spontaneous grassland recovery on abandoned croplands in northern China: Different vegetation patterns in desert and typical steppe.

Passive restoration (without any intervention) has been proposed as an effective strategy for grassland restoration in abandoned croplands. However, whether the vegetation in abandoned croplands can change towards the desired state and the time needed to reach a relative stable state are context-dependent. We investigated three abandoned croplands with different recovery times (5, 15 and 20 years) and one natural grassland in each of two different types of steppe (desert steppe and typical steppe) in the agro-pastoral ecotone of northern China to assess the restoration potential of grassland on abandoned croplands. Above- and below-ground biomass as well as species biodiversity increased gradually with increasing recovery time. After 20 years of restoration there was no significant difference between abandoned cropland and natural steppe in the typical steppe site, but above- and below-ground biomass and species biodiversity were still lower in abandoned cropland in the desert steppe site. At the beginning of restoration, the communities were dominated mainly by annual species, especially in the desert steppe. As recovery time increased, the biomass and richness of perennial graminoids and forbs increased significantly and replaced annual species as the dominant species. In both desert steppe and typical steppes, species similarity between restored and natural steppe increased over time, suggesting that previously cultivated grassland recovered towards the desired state. Our results indicate that 20 years was sufficient time for the restoration of croplands in the typical steppe, but more time may be needed in the desert steppe.

Shape-controlled synthesis of the metal-organic framework MIL-125 towards a highly enhanced catalytic performance for the oxidative desulfurization of 4,6-dimethyldibenzothiophene.

Nanoscale MIL-125 crystals with truncated octahedral shape were synthesized through the coordination modulation method using butyric acid as the modulator. Compared to the traditional MIL-125 crystals with dominant {001} facets, the truncated octahedral crystals are dominated by {101} facets as the exposed crystal facets. The MIL-125 crystals with different morphology exhibit significant facet-dependent catalytic activity for the oxidative desulfurization (ODS) reaction. The truncated octahedral MIL-125 crystals show remarkably enhanced catalytic performance towards oxidative desulfurization of the most refractory sulfur compound 4,6-dimethyldibenzothiophene (4,6-DMDBT). It is demonstrated that the shape-controlled synthesis of nanoscale MIL-125 is a promising way to promote its catalytic performance towards the reaction involving bulky molecules.

Synthesis of Aptamer-PEI-g-PEG Modified Gold Nanoparticles Loaded with Doxorubicin for Targeted Drug Delivery.

Due to drug resistance and toxicity in healthy cells, use of doxorubicin (DOX) has been limited in clinical cancer therapy. This protocol describes the designing of poly(ethylenimine) grafted with polyethylene glycol (PEI-g-PEG) copolymer functionalized gold nanoparticles (AuNPs) with loaded aptamer (AS1411) and DOX through amide reactions. AS1411 is specifically bonded with targeted nucleolin receptors on cancer cells so that DOX targets cancer cells instead of healthy cells. First, PEG is carboxylated, then grafted to branched PEI to obtain a PEI-g-PEG copolymer, which is confirmed by 1H NMR analysis. Next, PEI-g-PEG copolymer coated gold nanoparticles (PEI-g-PEG@AuNPs) are synthesized, and DOX and AS1411 are covalently bonded to AuNPs gradually via amide reactions. The diameter of the prepared AS1411-g-DOX-g-PEI-g-PEG@AuNPs is ~39.9 nm, with a zeta potential of -29.3 mV, indicating that the nanoparticles are stable in water and cell medium. Cell cytotoxicity assays show that the newly designed DOX loaded AuNPs are able to kill cancer cells (A549). This synthesis demonstrates the delicate arrangement of PEI-g-PEG copolymers, aptamers, and DOX on AuNPs that are achieved by sequential amide reactions. Such aptamer-PEI-g-PEG functionalized AuNPs provide a promising platform for targeted drug delivery in cancer therapy.

Comparative Transcriptional Profiling and Physiological Responses of Two Contrasting Oat Genotypes under Salt Stress.

Salinity is one of the major abiotic factors that affect productivity in oat. Here, we report a comparison of the transcriptomes of two hexaploid oat cultivars, 'Hanyou-5' and 'Huazao-2', which differ with respect to salt tolerance, in seedlings exposed to salt stress. Analysis of the assembled unigenes from the osmotically stressed and control libraries of 'Hanyou-5' and 'Huazao-2' showed that the expression of 21.92% (36,462/166,326) of the assembled unigenes was differentially regulated in the two cultivars after different durations of salt stress. Bioinformatics analysis showed that the main functional categories enriched in these DEGs were "metabolic process", "response to stresses", "plant hormone signal transduction", "MAPK signalling", "oxidative phosphorylation", and the plant-pathogen interaction pathway. Some regulatory genes, such as those encoding MYB, WRKY, bHLH, and zinc finger proteins, were also found to be differentially expressed under salt stress. Physiological measurements also detected significant differences in the activities of POD (76.24 ± 1.07 Vs 81.53 ± 1.47 U/g FW) in the two genotypes in response to osmotic stress. Furthermore, differential expression of 18 of these genes was successfully validated using RNA-seq and qRT-PCR analyses. A number of stress-responsive genes were identified in both cultivars, and candidate genes with potential roles in the adaptation to salinity were proposed.

Transcriptome analysis of hexaploid hulless oat in response to salinity stress.

BACKGROUND: Oat is a cereal crop of global importance used for food, feed, and forage. Understanding salinity stress tolerance mechanisms in plants is an important step towards generating crop varieties that can cope with environmental stresses. To date, little is known about the salt tolerance of oat at the molecular level. To better understand the molecular mechanisms underlying salt tolerance in oat, we investigated the transcriptomes of control and salt-treated oat using RNA-Seq. RESULTS: Using Illumina HiSeq 4000 platform, we generated 72,291,032 and 356,891,432 reads from non-stressed control and salt-stressed oat, respectively. Assembly of 64 Gb raw sequence data yielded 128,414 putative unique transcripts with an average length of 1,189 bp. Analysis of the assembled unigenes from the salt stressed and control libraries indicated that about 65,000 unigenes were differentially expressed at different stages. Functional annotation showed that ABC transporters, plant hormone signal transduction, plant-pathogen interactions, starch and sucrose metabolism, arginine and proline metabolism, and other secondary metabolite pathways were enriched under salt stress. Based on the RPKM values of assembled unigenes, 24 differentially expressed genes under salt stress were selected for quantitative RT-PCR validation, which successfully confirmed the results of RNA-Seq. Furthermore, we identified 18,039 simple sequence repeats, which may help further elucidate salt tolerance mechanisms in oat. CONCLUSIONS: Our global survey of transcriptome profiles of oat plants in response to salt stress provides useful insights into the molecular mechanisms underlying salt tolerance in this crop. These findings also represent a rich resource for further analysis of salt tolerance and for breeding oat with improved salt tolerance through the use of salt-related genes.

Transcriptome analysis of filling stage seeds among three buckwheat species with emphasis on rutin accumulation.

Buckwheat is an important minor crop with pharmaceutical functions due to rutin enrichment in the seed. Seeds of common buckwheat cultivars (Fagopyrum esculentum, Fes) usually have much lower rutin content than tartary buckwheat (F. tartaricum, Ft). We previously found a wild species of common buckwheat (F. esculentum ssp. ancestrale, Fea), with seeds that are high in rutin, similar to Ft. In the present study, we investigated the mechanism by which rutin production varies among different buckwheat cultivars, Fea, a Ft variety (Xide) and a Fes variety (No.2 Pingqiao) using RNA sequencing of filling stage seeds. Sequencing data generated approximately 43.78-Gb of clean bases, all these data were pooled together and assembled 180,568 transcripts, and 109,952 unigenes. We established seed gene expression profiles of each buckwheat sample and assessed genes involved in flavonoid biosynthesis, storage proteins production, CYP450 family, starch and sucrose metabolism, and transcription factors. Differentially expressed genes between Fea and Fes were further analyzed due to their close relationship than with Ft. Expression levels of flavonoid biosynthesis gene FLS1 (Flavonol synthase 1) were similar in Fea and Ft, and much higher than in Fes, which was validated by qRT-PCR. This suggests that FLS1 transcript levels may be associated with rutin accumulation in filling stage seeds of buckwheat species. Further, we explored transcription factors by iTAK, and multiple gene families were identified as being involved in the coordinate regulation of metabolism and development. Our extensive transcriptomic data sets provide a complete description of metabolically related genes that are differentially expressed in filling stage buckwheat seeds and suggests that FLS1 is a key controller of rutin synthesis in buckwheat species. FLS1 can effectively convert dihydroflavonoids into flavonol products. These findings provide a basis for further studies of flavonoid biosynthesis in buckwheat breeding to help accelerate flavonoid metabolic engineering that would increase rutin content in cultivars of common buckwheat.

Steric-Structure-Dependent Gel Formation, Hierarchical Structures, Rheological Behavior, and Surface Wettability.

A series of bicholesteryl-based gelators with different central linker atoms C, N, and O (abbreviated to GC, GN, and GO, respectively) have been designed and synthesized. The self-assembly processes of these gelators were investigated by using gelation tests, field-emission scanning electron microscopy, field-emission transmission electron microscopy, UV/Vis absorption, IR spectroscopy, X-ray diffraction, rheology, and contact-angle experiments. The gelation ability, self-assembly morphology, rheological, and surface-wettability properties of these gelators strongly depend on the central linker atom of the gelator molecule. Specifically, GC and GN can form gels in three different solvents, whereas GO can only form a gel in N,N-dimethylformamide (DMF). Morphologies from nanofibers and nanosheets to nanospheres and nanotubes can be obtained with different central atoms. Gels of GC, GN, and GO formed in the same solvent (DMF) have different tolerances to external forces. All xerogels gave a hydrophobic surface with contact angles that ranged from 121 to 152°. Quantum-chemical calculations indicate that the GC, GN, and GO molecules have very different steric structures. The results demonstrate that the central linker atom can efficiently modulate the molecular steric structure and thus regulate the supramolecular self-assembly process and properties of gelators.

Construction of Highly Catalytic Porous TiOPC Nanocomposite Counter Electrodes for Dye-Sensitized Solar Cells.

Developing low-cost, durable, and highly catalytic counter electrode (CE) materials based on earth-abundant elements is essential for dye-sensitized solar cells (DSSCs). In this study, we report a highly active nanostructured compositional material, TiOPC, which contains titanium, oxygen, phosphorus, and carbon, for efficient CE in I3-/I- electrolyte. The TiOPC nanocomposites are prepared from carbon thermal transformation of TiP2O7 in an atmosphere of nitrogen at high temperature, and their catalytic performance is regulated by changing the carbon content in the nanocomposites. The TiOPC with appropriate 24.6 wt % carbon and porous structure exhibits an enhanced electrocatalytic activity in the reduction of I3-, providing a short-circuit current density of 16.64 mA cm-2, an open-circuit potential of 0.78 V, and an energy conversion efficiency of 8.65%. The photovoltaic performance of TiOPC CE-based DSSC is even superior to that of a Pt CE-based cell (13.80 mA cm-2, 0.79 V, and 6.66%). The enhanced catalytic activity of TiOPC is attributed to the presence of predominant Ti-O-P-C structure along with the continuous conductive carbon network and the porous structure.

Development of microsatellite markers from tartary buckwheat.

A genomic library enriched with (gT)(n) repeats from tartary buckwheat (Fagopyrum tataricum) was constructed using 5'-anchored PCR for the development of microsattellite markers. Sequencing analysis of 5 clones from the library showed that they all contained microsatellites (totally 10 loci), and each was unique. An additional locus-specific primer was designed according to flanking sequence. Two of the microsatellite loci of 10 tartary buckwheat varieties were amplified using an anchored primer and a locus-specific primer, which revealed a clear polymorphic pattern. The data confirmed that the degenerate primer was reliably anchoring at the 5'-end of the microsatellite, and the primers developed based on this technology could be used for diversity analysis of tartary buckwheat.