Functional molecule-mediated assembled copper nanozymes for diabetic wound healing.

BACKGROUND: The complex hyperglycemic, hypoxic, and reactive oxygen species microenvironment of diabetic wound leads to vascular defects and bacterial growth and current treatment options are relatively limited by their poor efficacy. RESULTS: Herein, a functional molecule-mediated copper ions co-assembled strategy was constructed for collaborative treatment of diabetic wounds. Firstly, a functional small molecule 2,5-dimercaptoterephthalic acid (DCA) which has symmetrical carboxyl and sulfhydryl structure, was selected for the first time to assisted co-assembly of copper ions to produce multifunctional nanozymes (Cu-DCA NZs). Secondly, the Cu-DCA NZs have excellent multicatalytic activity, and photothermal response under 808 nm irradiation. In vitro and in vivo experiments showed that it not only could efficiently inhibit bacterial growth though photothermal therapy, but also could catalyze the conversion of intracellular hydrogen peroxide to oxygen which relieves wound hypoxia and improving inflammatory accumulation. More importantly, the slow release of copper ions could accelerate cellular proliferation, migration and angiogenesis, synergistically promote the healing of diabetic wound furtherly. CONCLUSIONS: The above results indicate that this multifunctional nanozymes Cu-DCA NZs may be a potential nanotherapeutic strategy for diabetic wound healing.

Multifunctional nanocomposites mediated novel hydrogel for diabetic wound repair.

The regeneration and repair of diabetic wounds, especially those including bacterial infection, have always been difficult and challenging using current treatment. Herein, an effective strategy is reported for constructing glucose-responsive functional hydrogels using nanocomposites as nodes. In fact, tannic acid (TA)-modified ceria nanocomposites (CNPs) and a zinc metal-organic framework (ZIF-8) were employed as nodes. Subsequent crosslinking with 3-acrylamidophenylboronic acid achieved functional nanocomposite-hydrogels (TA@CN gel, TA@ZMG gel) by radical-mediated polymerization. Compared with a simple physically mixed hydrogel system, the mechanical properties of TA@CN gel and TA@ZMG gel are significantly enhanced due to the intervention of the nanocomposite nodes. In addition, this kind of nanocomposite hydrogel can realize the programmed loading of drugs and release of drugs in response to glucose/PH, to coordinate and promote its application in the regeneration and repair of diabetic wounds and infected diabetic wounds. Specifically, TA@CN gel can remove reactive oxygen species and generate oxygen through its various enzymatic activities. At the same time, it can effectively promote neovascularization, thus promoting the regeneration and repair of diabetic wounds. Furthermore, glucose oxidase-loaded TA@ZMG gel exhibits glucose response and pH-regulating functions, triggering programmed metformin (Met) release by degrading the metal-organic framework (MOF) backbone. It also exhibited additional synergistic effects of antibacterial activity, hair regeneration and systemic blood glucose regulation, which make it suitable for the repair of more complex infected diabetic wounds. Overall, this novel nanocomposite-mediated hydrogel holds great potential as a biomaterial for the healing of chronic diabetic wounds, opening up new avenues for further biomedical applications.

A hydrogel system for drug loading toward the synergistic application of reductive/heat-sensitive drugs.

The preparation of hydrogels as drug carriers via radical-mediated polymerization has significant prospects, but the strong oxidizing ability of radicals and the high temperatures generated by the vigorous reactions limits the loading for reducing/heat-sensitive drugs. Herein, an applicable hydrogel synthesized by radical-mediated polymerization is reported for the loading and synergistic application of specific drugs. First, the desired sol is obtained by polymerizing functional monomers using a radical initiator, and then tannic-acid-assisted specific drug mediates sol-branched phenylboric acid group to form the required functional hydrogel (New-gel). Compared with the conventional single-step radical-mediated drug-loading hydrogel, the New-gel not only has better chemical/physical properties but also efficiently loads and releases drugs and maintains drug activity. Particularly, the New-gel has excellent loading capacity for oxygen, and exhibits significant practical therapeutic effects for diabetic wound repair. Furthermore, owing to its high light transmittance, the New-gel synergistically promotes the antibacterial effect of photosensitive drugs. This gelation strategy for loading drugs has further promising biomedical applications.

Copper-rich multifunctional Prussian blue nanozymes for infected wound healing.

The healing process of infected wounds was limited by bacterial infection, excessive reactive oxygen species (ROS) accumulation, and tissue hypoxia. In order to alleviate the above situations, herein, a copper-rich multifunctional ultra-small Prussian blue nanozymes (HPP@Cu NZs) was constructed for infected wound synergistic treatment. Firstly, hyaluronic acid was modified by branched polyethyleneimine which could form a complex with copper ions, to construct copper-rich Prussian blue nanozymes. Secondly, the HPP@Cu NZs have a uniform ultra-small nano size and excellent photothermal response performance, exhibition of multifunctional enzymatic activity and anti-inflammatory properties. Finally, the slow release of copper ions in the HPP@Cu NZs could effectively promote the formation of new blood vessels, thus giving it multifunctional properties. In vitro and in vivo experiments showed that it not only could effectively inhibit and kill bacteria under 808 nm near-infrared laser but also could remove excessive ROS, regulate oxygen levels, and anti-inflammation. More importantly, the release of copper ions could synergistically promote the healing of infected wounds as well as good biocompatibility. Overall, our studies provide a multifunctional strategy for infected wounds with synergistic treatment based on carrier construction.

Analysis of Rapid Rehabilitation Effect of Children with Severe Viral Encephalitis Based on Continuous Nursing of Omaha System.

Objective: To establish the Omaha System-based intensive care of children with viral encephalitis, compared with the conventional nursing applied in children with severe viral encephalitis for children with clinical symptoms, motor function, the incidence of complications, and the influence of quality of life, to intensive care of children with viral encephalitis way provide certain scientific basis. Methods: 62 cases of severe viral encephalitis diagnosed and treated in our hospital from X month 20XX to X month 20XX were randomly divided into 31 cases of intervention group and 31 cases of control group. The control group received routine nursing, and the intervention group added Omaha system on the basis of the control group. The recovery time of clinical symptoms and signs, FMA score, disability rate, quality of life, and satisfaction of family members were compared between the two groups. Results: The recovery time of clinical signs in the intervention group was shorter than that in the control group. Motor function was improved in both groups, but the improvement effect in intervention group was higher than that in control group. The quality of life in both groups was improved 1-3 months after discharge, but the improvement effect in the intervention group was higher than that in the control group. The incidence of physical dysfunction and behavioral abnormalities was low in the pregroup, and the difference between the two groups was statistically significant (P < 0.05), but the difference between language impairment and intellectual impairment was not statistically significant (P > 0.05). Conclusion: Omaha system nursing can significantly reduce the recovery time of clinical signs, improve FMA score, reduce disability rate, and improve the quality of life and family satisfaction of children with severe viral encephalitis.

A Low-Temperature Solution-Processed CuSCN/Polymer Hole Transporting Layer Enables High Efficiency for Organic Solar Cells.

The hole transporting layers (HTLs) between the electrode and light absorber play a vital role in charge extraction and transport processes in organic solar cells (OSCs). Herein, a bilayer structure HTL of CuSCN/TFB is formed by soluble copper(I) thiocyanate (CuSCN) and poly[(9,9-dioctylfluorenyl-2,7-diyl)-alt-(4,4'-(N-(4-butylphenyl)))] (TFB). The excellent charge extraction capability is proved in nonfullerene PM6:Y6 and fullerene PTB7-Th:PC71BM blend system-based cells. The introduction of TFB tunes the work function and polishes the interfacial contact between the HTL and light absorber, which favors the hole extraction process in cells. Meanwhile, lower recombination loss, higher exciton dissociation probability, and larger domain size are observed in CuSCN/TFB HTL-based cells compared to those of the reference cell with the pristine CuSCN HTL, which significantly improve the photovoltaic performance. As a result, a champion efficiency of 15.10% is obtained, which is >14% higher than the efficiency of 13.15% obtained in the reference cell. This study suggests that CuSCN/TFB is a promising HTL to achieve high efficiency for OSCs.

Solution-Processable ZnO/Carbon Quantum Dots Electron Extraction Layer for Highly Efficient Polymer Solar Cells.

In this work, we report the effort to develop high-efficiency inverted polymer solar cells (PSCs) by applying a solution-processable bilayer ZnO/carbon quantum dots (C-QDs) electron extraction layer (EEL). It is shown that the use of the bilayer EEL helps to suppress the exciton quenching by passivating the ZnO surface defects in the EEL, leading to an enhanced exciton dissociation, reduced charge recombination and more efficient charge extraction probability, and thereby achieving high power conversion efficiency (PCE). The inverted PSCs, based on the blend of poly{4,8-bis[(2-ethylhexyl)oxy]benzo[1,2-b:4,5-b']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)carbonyl]thieno[3,4-b]thiophene-4,6-diyl} and [6,6]-phenyl C71-butyric acid methyl ester, possess a significant improvement in PCE of ∼9.64%, which is >27% higher than that of a control cell (∼7.59%). The use of a bilayer ZnO/C-QD EEL offers a promising approach for attaining high-efficiency inverted PSCs.