RESUMO
The Tsix regulatory region was examined in vole Microtus rossiaemeridionalis. The minimal promoter region, three potential enhancer regulatory elements and one transcription suppressor element were identified. The enhancer regions contained potential binding sites of transcription activators, while in the region of putative silencer contained potential binding site of the ARP1 (NR2F2) protein. This protein can play the role of either activator or repressor depending on the promoter context.
Assuntos
Arvicolinae/genética , RNA não Traduzido/genética , Elementos de Resposta/genética , Elementos Silenciadores Transcricionais/genética , Animais , Fator II de Transcrição COUP/genética , Proteínas Repressoras/genéticaRESUMO
Infection of young domestic cats by cowpox virus isolated from sick rodents (family Muridae) revealed their high susceptibility to the virus; a severe disease with 100% lethality developed after oral inoculation as well as upon skin scarification. The disease in dermally infected animals was accompanied by eruptions on the site of inoculation. High concentration of the virus was detected in lungs of animals infected by either of inoculation routes. The data testify the possible participation of domestic cats as intermediate hosts in the circulation of cowpox virus.
Assuntos
Gatos/microbiologia , Vírus da Varíola Bovina/crescimento & desenvolvimento , Animais , Varíola Bovina/transmissão , Replicação ViralRESUMO
The presented studies were performed at the WHO Collaboration Center for Smallpox in Moscow in the framework of the WHO monkey pox project. The authors recommend improved methods for rapid detection of orthopoxvirus antigen, namely passive haemagglutination (PHA) using dried stable red blood cells and ELISA in order to provide more rapid and efficient laboratory diagnosis under field conditions. Independent serologic diagnosis of monkey pox by ELISA-adsorption (ELISA-A) was proved of value for epidemiological studies and for detection of inapparent infections. The application range of the latter technique and its limitations were also determined.
Assuntos
Monkeypox virus/isolamento & purificação , Poxviridae/isolamento & purificação , Anticorpos Antivirais/análise , Ensaio de Imunoadsorção Enzimática , Testes de Hemaglutinação , Humanos , Monkeypox virus/imunologiaRESUMO
The materials of work lasting for 11 years and including the study of 3,425 samples collected at different periods after the eradication of smallpox from patients suspected for orthopoxvirus infection indicate high effectiveness of electron microscopy and especially immune electron microscopy as an integral part of diagnostic examination.
Assuntos
Microscopia Eletrônica/métodos , Vigilância da População , Infecções por Poxviridae/diagnóstico , Varíola Bovina/diagnóstico , Varíola Bovina/epidemiologia , Diagnóstico Diferencial , Humanos , Microscopia Imunoeletrônica/métodos , Monkeypox virus , Poxviridae/ultraestrutura , Infecções por Poxviridae/epidemiologia , Varíola/diagnóstico , Varíola/epidemiologia , U.R.S.S./epidemiologia , Vírion/ultraestrutura , Organização Mundial da SaúdeRESUMO
The present work describes a case of human disease resulting from the bite of a white rat and caused by a biological variant of cowpox virus. The isolates obtained from the sick man and the white rats which had been the source of this infection proved to be identical and did not differ from the biological variants of cowpox virus, isolated earlier from white rats and carnivorous animals of the family Felidae. Thus, the possibility of ratpox (cowpox) transmission from sick rodents to man was established.
Assuntos
Doenças Profissionais/diagnóstico , Vacínia/diagnóstico , Adulto , Animais , Mordeduras e Picadas/complicações , Mordeduras e Picadas/microbiologia , Vetores de Doenças , Feminino , Humanos , Doenças Profissionais/microbiologia , Doenças Profissionais/transmissão , Ratos , Doenças dos Roedores/microbiologia , Doenças dos Roedores/transmissão , Fatores de Tempo , Vacínia/microbiologia , Vacínia/transmissão , Vacínia/veterinária , Vaccinia virus/isolamento & purificaçãoRESUMO
An enzyme immunoassay (EIA) system for the species-specific diagnosis of monkeypox, based on the use of monoclonal antibodies (McAb) to monkeypox virus, has been developed. Immunoglobulins, isolated from McAb-containing cultural and immune ascitic fluids, have been conjugated with horse-radish peroxidase and used as detector antibodies. For immunosorption, rabbit polyclonal antibodies to the vaccine virus have been used. The specificity and sensitivity of the EIA system thus obtained have been tested on animals and humans having monkeypox and confirmed by traditional diagnostic methods (the isolation of the virus on chick embryo chorioallantoic membranes and in cell culture).
Assuntos
Monkeypox virus/isolamento & purificação , Animais , Anticorpos Monoclonais , Antígenos Virais/análise , Embrião de Galinha , Estudos de Avaliação como Assunto , Humanos , Técnicas Imunoenzimáticas , Imunoglobulina G , Isotipos de Imunoglobulinas , Imunoglobulina M , Monkeypox virus/classificação , Monkeypox virus/imunologia , Especificidade da Espécie , Cultura de Vírus/métodosRESUMO
A virus, identified as cowpox virus by its biological properties and the results of the analysis of its DNA, was isolated from a sick 4-year-old child with a clinical picture of pox, though having had no contacts with known natural carriers of the causative agent of this infection. At the same time the isolated virus was found to differ from the reference strain, as well as from other isolates of vaccinia virus by some biological markers (and in particular by the structure of cytoplasmic inclusions of type A) and by the restriction profile of DNA. The Hind III maps indicating the location of restriction sites made it possible to localize the genome differences established in this study. The specific feature of this case was the previous close contact of the child with a mole which was probably the source of infection.
Assuntos
Vírus da Varíola Bovina/classificação , Varíola Bovina/virologia , Animais , Embrião de Galinha , Pré-Escolar , Chlorocebus aethiops , Varíola Bovina/patologia , Vírus da Varíola Bovina/genética , Vírus da Varíola Bovina/isolamento & purificação , Vírus da Varíola Bovina/patogenicidade , DNA Viral/genética , Feminino , Humanos , Camundongos , Microscopia Eletrônica , Ratos , Mapeamento por Restrição/métodos , Células VeroRESUMO
Results are presented on virological examination conducted on WHO request of a Naples infant mummy with smallpox-like lesions. Electron microscopy of lesions confirmed the findings of Italian researchers: well-preserved virus-like structures, whose size and morphology were identical to those of orthopoxviruses have been revealed. It was shown that the virus in mummy skin had lost its viability. Viral antigenic activity could not be detected in EIA or RPHA, nor was determined its DNA in the test of DNA molecular hybridization.