RESUMO
Over the past decade, solid-state cholesteric liquid crystals (CLCsolid ) have emerged as a promising photonic material, heralding new opportunities for the advancement of optical photonic biosensors and actuators. The periodic helical structure of CLCsolid s gives rise to their distinctive capability of selectively reflecting incident radiation, rendering them highly promising contenders for a wide spectrum of photonic applications. Extensive research is conducted on utilizing CLCsolid 's optical characteristics to create optical sensors for bioassays, diagnostics, and environmental monitoring. This review provides an overview of emerging technologies in the field of interpenetrating polymeric network-CLCsolid (IPN) and CLCsolid -based optical sensors, including their structural designs, processing, essential materials, working principles, and fabrication methodologies. The review concludes with a forward-looking perspective, addressing current challenges and potential trajectories for future research.
RESUMO
Aptamers are RNA/DNA oligonucleotide molecules that specifically bind to a targeted complementary molecule. As potential recognition elements with promising diagnostic and therapeutic applications, aptamers, such as monoclonal antibodies, could provide many treatment and diagnostic options for blood diseases. Aptamers present several superior features over antibodies, including a simple in vitro selection and production, ease of modification and conjugation, high stability, and low immunogenicity. Emerging as promising alternatives to antibodies, aptamers could overcome the present limitations of monoclonal antibody therapy to provide novel diagnostic, therapeutic, and preventive treatments for blood diseases. Researchers in several biomedical areas, such as biomarker detection, diagnosis, imaging, and targeted therapy, have widely investigated aptamers, and several aptamers have been developed over the past two decades. One of these is the pegaptanib sodium injection, an aptamer-based therapeutic that functions as an anti-angiogenic medicine, and it is the first aptamer approved by the U.S. Food and Drug Administration (FDA) for therapeutic use. Several other aptamers are now in clinical trials. In this review, we highlight the current state of aptamers in the clinical trial program and introduce some promising aptamers currently in pre-clinical development for blood diseases.
Assuntos
Aptâmeros de NucleotídeosRESUMO
Collection of nasopharyngeal samples using swabs followed by the transfer of the virus into a solution and an RNA extraction step to perform reverse transcription polymerase chain reaction (PCR) is the primary method currently used for the diagnosis of COVID-19. However, the need for several reagents and steps and the high cost of PCR hinder its worldwide implementation to contain the outbreak. Here, we report a cotton-tipped electrochemical immunosensor for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus antigen. Unlike the reported approaches, we integrated the sample collection and detection tools into a single platform by coating screen-printed electrodes with absorbing cotton padding. The immunosensor was fabricated by immobilizing the virus nucleocapsid (N) protein on carbon nanofiber-modified screen-printed electrodes which were functionalized by diazonium electrografting. The detection of the virus antigen was achieved via swabbing followed by competitive assay using a fixed amount of N protein antibody in the solution. A square wave voltammetric technique was used for the detection. The limit of detection for our electrochemical biosensor was 0.8 pg/mL for SARS-CoV-2, indicating very good sensitivity for the sensor. The biosensor did not show significant cross-reactivity with other virus antigens such as influenza A and HCoV, indicating high selectivity of the method. Moreover, the biosensor was successfully applied for the detection of the virus antigen in spiked nasal samples showing excellent recovery percentages. Thus, our electrochemical immunosensor is a promising diagnostic tool for the direct rapid detection of the COVID-19 virus that requires no sample transfer or pretreatment.
Assuntos
COVID-19/diagnóstico , Fibra de Algodão , Técnicas Eletroquímicas/métodos , Imunoensaio/métodos , SARS-CoV-2/isolamento & purificação , Anticorpos Antivirais/imunologia , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Carbono/química , Proteínas do Nucleocapsídeo de Coronavírus/química , Proteínas do Nucleocapsídeo de Coronavírus/imunologia , Técnicas Eletroquímicas/instrumentação , Eletrodos , Gossypium/química , Humanos , Proteínas Imobilizadas/química , Proteínas Imobilizadas/imunologia , Imunoensaio/instrumentação , Limite de Detecção , Nanofibras/química , Fosfoproteínas/química , Fosfoproteínas/imunologia , SARS-CoV-2/imunologiaRESUMO
This study demonstrates the development of a sensitive, specific, and quantitative peptide-based nanoprobe prototype assay for the detection of Legionellaceae in a simple way and in a short time. In this work, proteases present in the culture supernatants of Legionella spp. were used as a biomarker. Fluorogenic peptide substrates, specific to Legionella strains culture supernatant proteases, were identified. Peptidases produced a significant increase in the fluorescence intensity following the cleavage of the dipeptide fluorogenic substrates. The specific substrates were identified and coupled with carboxyl-terminated nano-magnetic particles (NMPs). On the other hand, the C-terminal was conjugated with the cysteine residue to covalently integrate with a gold sensing platform via the Au-S linkage. Four different sensors were fabricated from the four specific substrates, which were treated with the protesase of six different species of Legionella. In the presence of specific protease, the peptide sequence is digested and the magnetic nanobeads moved out of the gold surface, resulting in the apparence of gold color. One of the nanoprobes sensitivity detects as low as 60 CFU mL-1 of Legionella anisa, Legionella micdadei, and Fluoribacter dumoffii. The cross-reactivity of the sensors was tested using other closely associated bacterial species and no significant cross-reactivity of the sensors was found. It is envisaged that this assay could be useful for screening purposes or might be supportive for the fast and easy detection of Legionella protease activity for water monitoring purposes.
Assuntos
Técnicas Biossensoriais , Legionellaceae , Legionella , PeptídeosRESUMO
Aptamers are short single-stranded oligonucleotides (either DNA or RNA) that can fold into well-defined three-dimensional (3D) spatial structures which enable them to capture their specific target by complementary shape interactions. Aptamers are selected from large random libraries through the SELEX process and only a small fraction of the sequence is involved in direct docking with the target. In this paper, we describe the possible truncation variants of zearalenone (ZEA) aptamer which might be an effective binding region for the target. The originally selected zearalenone (ZEA) aptamer was 80-mer in length and shown to bind the target with a high affinity (Kd = 41 ± 5 nM). Herein, computational docking simulation was performed with 15 truncated variants to determine the predicted binding energy and responsible binding site of the aptamer-analyte complex. The results revealed that 5 truncated variants had binding energy lower than - 7.0 kcal/mol. Circular dichroism analysis was performed on the shortlisted aptamer and the conformational change of aptamers was observed with the presence of an analyte. Aptamer Z3IN (29-mer) was chosen as the most enhanced affinity for its target with a dissociation constant of 11.77 ± 1.44 nM. The aptamer was further applied in the electrochemical aptasensor of ZEA based on an indirect competitive format. The results demonstrated that the truncated aptamer leads to an enhancement of the sensitivity of the biosensor.
Assuntos
Aptâmeros de Nucleotídeos/análise , Técnicas Eletroquímicas/instrumentação , Zearalenona/análise , Aptâmeros de Nucleotídeos/química , Sequência de Bases , Técnicas Biossensoriais/métodos , Dicroísmo Circular , Espectroscopia Dielétrica , Limite de Detecção , Simulação de Acoplamento MolecularRESUMO
Since the COVID-19 disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) was declared a pandemic, it has spread rapidly, causing one of the most serious outbreaks in the last century. Reliable and rapid diagnostic tests for COVID-19 are crucial to control and manage the outbreak. Here, a label-free square wave voltammetry-based biosensing platform for the detection of SARS-CoV-2 in nasopharyngeal samples is reported. The sensor was constructed on screen-printed carbon electrodes coated with gold nanoparticles. The electrodes were functionalized using 11-mercaptoundecanoic acid (MUA) which was used for the immobilization of an antibody against SARS-CoV-2 nucleocapsid protein (N protein). The binding of the immunosensor with the N protein caused a change in the electrochemical signal. The detection was realised by measuring the change in reduction peak current of a redox couple using square wave voltammetry at 0.04 V versus Ag ref. electrode on the immunosensor upon binding with the N protein. The electrochemical immunosensor showed high sensitivity with a linear range from 1.0 pg.mL-1 to 100 ng.mL-1 and a limit of detection of 0.4 pg.mL-1 for the N protein in PBS buffer pH 7.4. Moreover, the immunosensor did not exhibit significant response with other viruses such as HCoV, MERS-CoV, Flu A and Flu B, indicating the high selectivity of the sensor for SARS-CoV-2. However, cross reactivity of the biosensor with SARS-CoV is indicated, which gives ability of the sensor to detect both SARS-CoV and SARS-CoV-2. The biosensor was successfully applied to detect the SARS-CoV-2 virus in clinical samples showing good correlation between the biosensor response and the RT-PCR cycle threshold values. We believe that the capability of miniaturization, low-cost and fast response of the proposed label-free electrochemical immunosensor will facilitate the point-of-care diagnosis of COVID 19 and help prevent further spread of infection.
Assuntos
Teste para COVID-19/métodos , COVID-19/diagnóstico , Proteínas do Nucleocapsídeo de Coronavírus/análise , Técnicas Eletroquímicas/métodos , Imunoensaio/métodos , SARS-CoV-2/química , Anticorpos Imobilizados/imunologia , Técnicas Biossensoriais/instrumentação , Técnicas Biossensoriais/métodos , Teste para COVID-19/instrumentação , Carbono/química , Proteínas do Nucleocapsídeo de Coronavírus/imunologia , Técnicas Eletroquímicas/instrumentação , Eletrodos , Ácidos Graxos/química , Ouro/química , Humanos , Imunoensaio/instrumentação , Limite de Detecção , Nanopartículas Metálicas/química , Nasofaringe/virologia , Fosfoproteínas/análise , Fosfoproteínas/imunologia , Compostos de Sulfidrila/químicaRESUMO
The novel corona (SARS-CoV-2) virus causes a global pandemic, which motivates researchers to develop reliable and effective methods for screening and detection of SARS-CoV-2. Though there are several methods available for the diagnosis of SARS-CoV-2 such as RT-PCR and ELSIA, nevertheless, these methods are time-consuming and may not apply at the point of care. In this study, we have developed a specific, sensitive, quantitative and fast detection method for SARS-CoV-2 by fluorescence resonance energy transfer (FRET) assay. The total extracellular protease proteolytic activity from the virus has been used as the biomarker. The specific peptide sequences from the library of 115 dipeptides were identified via changes in the fluorescence signal. The fluorogenic dipeptide substrates have the fluorophore and a quencher at the N- and the C- terminals, respectively. When the protease hydrolyzes the peptide bond between the two specific amino acids, it leads to a significant increase in the fluorescence signals. The specific fluorogenic peptide (H-d) produces a high fluorescence signal. A calibration plot was obtained from the changes in the fluorescence intensity against the different concentrations of the viral protease. The lowest limit of detection of this method was 9.7 ± 3 pfu/mL. The cross-reactivity of the SARS-CoV-2-specific peptide was tested against the MERS-CoV which does not affect the fluorescence signal. A significant change in the fluorescence signal with patient samples indicates that this FRET-based assay might be applied for the diagnosis of SARS-CoV-2 patients. Graphical abstract.
Assuntos
Teste para COVID-19/métodos , COVID-19/diagnóstico , Proteases 3C de Coronavírus/metabolismo , Corantes Fluorescentes/metabolismo , Peptídeos/metabolismo , SARS-CoV-2 , Proteínas Virais/metabolismo , Animais , Bioensaio , COVID-19/microbiologia , Chlorocebus aethiops , Transferência Ressonante de Energia de Fluorescência , Humanos , Biblioteca de Peptídeos , SARS-CoV-2/genética , SARS-CoV-2/metabolismo , Células Vero , Ensaio de Placa ViralRESUMO
Point-of-care facile and economical detection of Staphylococcus aureus (S. aureus), one of the main causes of food-borne illness, is highly demanded for the early diagnosis and control of infections. Herein, inspired by the proteolytic activity of S. aureus protease on a specific peptide substrate, we developed a rapid, simple and cost-effective biosensor for S. aureus using dual colorimetric and electrochemical detection on the same platform. In this approach, gold screen printed electrodes were used on which specific peptide sequences coupled to magnetic nanoparticles were immobilized giving the black color of the sensor surface. The addition of the S. aureus protease solution on the electrode surface causes cleavage of the peptide sequence and the release of the magnetic nanoparticles revealing the golden colour of the electrode which can be easily seen by the naked eye. Furthermore, square wave voltammetric signals can be detected on the same electrode in the ferro/ferricyanide redox couple. The change in the peak current after peptide cleavage was directly proportional to the concentration of S. aureus. The detection limit of the electrochemical assay was 3 CFU ml-1 after 1 min. Moreover, the biosensor was capable of specifically distinguishing S. aureus from other food- and water-borne bacteria such as E. coli and Listeria using the dual mode colorimetric and electrochemical detection. The biosensor was also tested in spiked milk and water samples showing very good recovery percentages. Thus, we believe that this dual mode biosensing platform enables the easy and accurate determination of S. aureus and holds great promise for point-of-care diagnosis.
Assuntos
Técnicas Biossensoriais/métodos , Colorimetria/métodos , Técnicas Eletroquímicas/métodos , Nanopartículas/química , Staphylococcus aureus/isolamento & purificação , Animais , Técnicas Eletroquímicas/instrumentação , Eletrodos , Contaminação de Alimentos/análise , Ouro/química , Proteínas Imobilizadas/química , Proteínas Imobilizadas/metabolismo , Limite de Detecção , Fenômenos Magnéticos , Metaloendopeptidases/metabolismo , Leite/química , Oligopeptídeos/química , Staphylococcus aureus/enzimologia , Staphylococcus aureus/metabolismo , Microbiologia da ÁguaRESUMO
Cylindrospermopsin (CYN) is one of the most concerning cyanotoxins due to its potential toxicity and spreading to various environments including drinking water. CYN has potential interferences with human and animal metabolic pathways, which influence the functions of organs including liver, kidneys, lungs, etc. CYN is involved in the inhibition of protein synthesis and detachment of ribosomes from the endoplasmic reticulum membrane. It also interacts with soluble proteins, which are associated with protein translations. It is believed that cytochrome 450 is responsible for the rapid toxicity of CYN. Researchers are urged to develop a high-throughput screening method for the detection of CYN in water. Construction of low cost, rapid, and sensitive analytical methods for the detection of CYN is challenging. Here, we used graphene oxide (GO) as the fluorescence sensing platform for probing the high affinity of the short aptamer derived from the wild-type long aptamer-CYN sensing. The biosensor construction involved two steps: first, quenching the fluorescence of fluorescent-labelled truncated aptamer using GO as a quencher and, second, fluorescence recovery in the presence of CYN by competitive binding between the target and GO. One of the truncate aptamers has a 12-fold higher affinity and enhances sensitivity compared to the long aptamer sequence. The limit of detection of the high affinity truncated aptamer is 17 pM which is 6-fold lower than the long aptamer (100 pM). The sensor specifically detects CYN in the presence of other potential interfering toxins. The performance of the sensor was validated using CYN spiked tap water with very good recovery percentage. A rapid and highly sensitive detection of CYN from water resources has been achieved using this method.
Assuntos
Alcaloides/análise , Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Grafite/química , Poluentes Químicos da Água/análise , Sítios de Ligação , Toxinas de Cianobactérias , Água Potável/análise , Corantes Fluorescentes/química , Limite de DetecçãoRESUMO
A novel electrochemical biosensor is reported for simultaneous detection of two of the most common food-borne pathogens: Listeria monocytogenes and Staphylococcus aureus. The biosensor is composed of an array of gold nanoparticles-modified screen-printed carbon electrodes on which magnetic nanoparticles coupled to specific peptides were immobilized via streptavidin-biotin interaction. Taking advantage of the proteolytic activities of the protease enzymes produced from the two bacteria on the specific peptides, the detection was achieved in 1 min. The detection was realized by measuring the percentage increase of the square wave voltammetric peak current at 0.1 V versus a Ag/AgCl reference electrode in ferro/ferricyanide redox couple after incubation with the bacteria protease. The integration of the specificity of the bacterial enzymes towards their peptide substrates with the sensitivity of the electrochemical detection on the sensor array allows the rapid, sensitive and selective quantification of the two bacteria. Outstanding sensitivities were achieved using this biosensor array platform with limit of detection of 9 CFU mL-1 for Listeria monocytogenes and 3 CFU mL-1 for Staphylococcus aureus. The multiplexing capability and selectivity of the array voltammetric biosensor were demonstrated by analysing samples of Staphylococcus aureus, Listeria monocytogenes or E. coli and also containing a mixture of two or three bacteria. Using this biosensor, the two bacteria were successfully quantified simultaneously in one step without the need for DNA extraction or amplification techniques. This platform offers promise for rapid, simple and cost-effective simultaneous detection of various bacteria. Graphical abstract.
Assuntos
Proteínas de Bactérias/análise , Técnicas Biossensoriais/métodos , Listeria monocytogenes/isolamento & purificação , Peptídeo Hidrolases/análise , Peptídeos/química , Staphylococcus aureus/isolamento & purificação , Proteínas de Bactérias/química , Técnicas Biossensoriais/instrumentação , Carbono/química , Técnicas Eletroquímicas/instrumentação , Técnicas Eletroquímicas/métodos , Eletrodos , Ouro/química , Proteínas Imobilizadas/química , Limite de Detecção , Listeria monocytogenes/enzimologia , Fenômenos Magnéticos , Nanopartículas Metálicas/química , Peptídeo Hidrolases/química , Proteólise , Staphylococcus aureus/enzimologiaRESUMO
An electrochemical aptasensor is described for determination of the phytohormone of zearalenone (ZEA). The gold electrode was modified with ZEA via covalent attachment using cysteamine-hydrochloride and 1,4-phenylene diisocyanate linker. A truncated ZEA aptamer with a dissociation constant of 13.4 ± 2.1 nM was used in an aptasensor. The electrochemical property was investigated using square wave voltammetry for monitoring the change in the electron transfer using the ferro/ferricyanide system as redox probe. Under optimal experimental conditions, the response was best measured at a potential of 0.20 V (vs. Ag/AgCl). The signals depended on the competitive mechanism between the immobilised ZEA and free ZEA for the aptamer binding site. The aptasensor works in the range 0.01 to 1000 ng·mL-1 ZEA concentration, with a detection limit of 0.017 ng·mL-1. High degree of cross-reactivity with the other analogues of ZEA was observed, whereas none towards other mycotoxins. The aptasensor was further applied for the determination of ZEA in the extract of maize grain and showed good recovery percentages between 87 and 110%. Graphical abstract Schematic representation of the electrochemical determination of zearalenone based on indirect competitive assay. Step a Immobilisation of ZEA on the surface of gold electrode via covalent attachment, b competition for the ZEA aptamer binding site between immobilised and free ZEA, and c current signal of the binding event based on SWV technique.
Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Micotoxinas/análise , Zearalenona/análise , Sequência de Bases , Técnicas Eletroquímicas/instrumentação , Eletrodos , Contaminação de Alimentos/análise , Ouro/química , Ácidos Nucleicos Imobilizados/química , Limite de Detecção , Micotoxinas/química , Zea mays/química , Zearalenona/químicaRESUMO
: Detecting tissue pH in vivo is extremely vital for medical diagnosis and formulation of treatment decisions. To this end, many investigations have been carried out to develop an accurate and efficient method of in vivo pH measurement. Most of the techniques developed so far suffer from inadequate accuracy, due to poor sensitivity at low concentration of the target or nonspecific interactions within the tissue matrix. To overcome these issues, we describe herein the development of a simple, yet reliable, way to estimate pH with high precision using a Gd(III)-DOTA-silyl-based acid-labile group as a pH-sensitive contrast agent with Magnetic Resonance Imaging (MRI). With this method, a change in T1 weighted image intensity of the newly developed pH-sensitive contrast is directly linked to the proton concentration in the media. As a result, we were able estimate the pH of the target with 95% reliability.
Assuntos
Meios de Contraste/química , Gadolínio/química , Concentração de Íons de Hidrogênio , Imageamento por Ressonância Magnética , Animais , Técnicas de Química Sintética , Meios de Contraste/síntese química , Meios de Contraste/metabolismo , Modelos Animais de Doenças , Gadolínio/metabolismo , Humanos , Imageamento por Ressonância Magnética/métodos , Camundongos , Modelos Químicos , Estrutura Molecular , Neoplasias/diagnóstico por imagem , Neoplasias/metabolismo , Especificidade de Órgãos , Distribuição TecidualRESUMO
The surface protein overexpressed on cancer cells can be used as biomarkers for early detection of specific diseases. Anti-VCAM-1 and anti-IL4Rα DNA aptamers specific to VCAM-1 and IL4Rα receptors that are overexpressed in 4T1 tumor-bearing mice could be used as potential biomarker for both diagnostic and therapeutic applications in cancer biology. Cell Viability and luciferase assay of 4T1-Luc2 cancer cells in the presence of anti-VCAM-1 ssDNA or anti-IL4Rα RNA aptamers was assessed by monitoring the changes in the absorbance and the fluorescence of Alamar blue dye. The aptamer-conjugated SPIO magnetic beads, used for the selective targeting to tumor sites, were monitored using noninvasive MRI and Bioluminescence imaging (BLI). Cell viability and luciferase assays showed that both anti-VCAM-1 and anti-IL4Rα aptamers favor the depletion of cancer cells and limit tumor progression. Microscopic analyses confirmed that the target specific aptamers significantly trigger tumor cell apoptosis and limit cancer cell growth in vitro. The intravenous injection of SPIO nanoparticle-conjugated aptamers were further confirmed using noninvasive MRI and Bioluminescence imaging. Anti-VCAM1 and anti-IL4Rα aptamers, specific to VCAM-1 and IL4Rα receptors overexpressed in 4T1-Luc2 tumor-bearing mice, were used as diagnostic and therapeutic tools.
Assuntos
Aptâmeros de Nucleotídeos/administração & dosagem , Neoplasias da Mama/diagnóstico , Neoplasias da Mama/terapia , Subunidade alfa de Receptor de Interleucina-4/antagonistas & inibidores , Nanopartículas Magnéticas de Óxido de Ferro , Nanomedicina Teranóstica , Molécula 1 de Adesão de Célula Vascular/antagonistas & inibidores , Animais , Apoptose/efeitos dos fármacos , Aptâmeros de Nucleotídeos/química , Neoplasias da Mama/etiologia , Neoplasias da Mama/patologia , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Modelos Animais de Doenças , Feminino , Genes Reporter , Humanos , Medições Luminescentes , Nanopartículas Magnéticas de Óxido de Ferro/química , Imageamento por Ressonância Magnética , Camundongos , Nanomedicina Teranóstica/métodos , Ensaios Antitumorais Modelo de XenoenxertoRESUMO
A colorimetric assay is presented for the detection of Stachybotrys chartarum proteases as biomarkers. The assay comprises a gold film acting as solid support and carrying an immobilized peptide substrate that is specific for S. chartarum protease. The substrate was conjugated to black magnetic nanoparticles (MNPs) to form a monolayer on the gold film. Hence, detection nanoprobe is black. If, however, the peptide-MNP fragments are cleaved by S. chartarum proteases present in a sample, the golden color of the detecting nanoprobe becomes apparent so that positive visual readout is enabled. The method was applied to the determination of S. chartarum in (spiked) environmental samples. The limit of detection ranges from 10 to 100 spores·mL-1 depending on the kind of sample (culture, dust, mold and soil). Assay specificity was examined for Aspergillus flavus, Fusarium solani. Penicillin chrysogenum, and Saccharomyces cerevisiae, and negative readouts were observed visually for all samples, except for those also containing S. chartarum. Graphical abstract Schematic presentation of S. chartarum colorimetric nanoprobe.
Assuntos
Colorimetria/métodos , Nanopartículas de Magnetita/química , Papel , Peptídeo Hidrolases/análise , Peptídeos/química , Stachybotrys/isolamento & purificação , Biomarcadores/análise , Colorimetria/instrumentação , Poeira , Microbiologia Ambiental , Proteínas Fúngicas/análise , Ouro/química , Limite de Detecção , Estudo de Prova de Conceito , Microbiologia do Solo , Stachybotrys/enzimologiaRESUMO
Heroin, marijuana and cocaine are widely abused drugs. Their use can be readily detected by analyzing urine for the metabolites morphine (MOR), tetrahydrocannabinol (THC) or benzoylecgonine (BZC). A multiplex immunosensor is described here for detection of MOR, THC and BZC using screen printed carbon array electrodes modified with gold nanoparticles. Antibodies against MOR, THC and BZC were immobilized on eight electrodes in a sensor array simultaneously, and a competitive assay was used for the detection. The free analytes in the sample compete with bovine serum albumin-conjugated analytes for the immobilized antibodies on the sensor surface. The array is capable of detecting the three drugs simultaneously within 20-40 min. The method has a high sensitivity, with detection limits as low as 1.2, 7.0, and 8.0 pg.mL-1 for MOR, THC and BZC, respectively. Cross reactivity testing was preformed to monitor any nonspecific binding. The results revealed good selectivity. Urine samples were spiked with the 3 drugs and tested with the multiplexed immunosensor. Recovery percentages ranged between 88 to 115%. Graphical abstract Schematic presentation of the multiplexed immunosensor for drugs of abuse,viz. tetrahydrocannabinol (THC), morphine (MOR), and benzoylecgonine (BZC)) by using an array of modified electrodes.
Assuntos
Cocaína/análogos & derivados , Dronabinol/urina , Drogas Ilícitas/urina , Morfina/urina , Anticorpos/química , Anticorpos/imunologia , Cocaína/imunologia , Cocaína/urina , Dronabinol/imunologia , Técnicas Eletroquímicas , Ouro/química , Humanos , Proteínas Imobilizadas/química , Proteínas Imobilizadas/imunologia , Imunoensaio , Limite de Detecção , Nanopartículas Metálicas/química , Morfina/imunologia , Detecção do Abuso de SubstânciasRESUMO
The high-affinity region of a truncated aptamer was applied to the development of a sensitive method for the determination of microcystin-LR (MC-LR) using competitive displacement and molecular beacons. In this assay, the fluorophore and quencher labelled complementary sequences of the aptamer are hybridized with the truncated aptamer to form a fluorophore-quencher pair. In the presence of MC-LR, the aptamer duplex dissociates, and the fluorophore-quencher pair is separated. This turn leads to an increase in the yellow fluorescence which is best measured at excitation/emission wavelengths of 555/580 nm. One of the truncated aptamers showed a 50-fold increase in the affinity (0.93 nM) compared to the wild type aptamer (50 nM). The truncated sequence shows considerable cross-reactivity with L congeners but none with other congeners. The assay works in 0.5 to 200 nM MC-LR concentration range. It was applied to spiked tap water samples and gave recoveries around 95 ± 5%. Graphical abstract Schematic representation of a method for determination of microcystin-LR via fluorescence that is induced by competitive displacement of complementary DNA strands in a truncated dsDNA aptamer.
Assuntos
Aptâmeros de Nucleotídeos/química , Técnicas Biossensoriais/métodos , DNA/química , Microcistinas/análise , Espectrometria de Fluorescência/métodos , Aptâmeros de Nucleotídeos/genética , Aptâmeros de Nucleotídeos/metabolismo , DNA/genética , Água Potável/análise , Corantes Fluorescentes/química , Limite de Detecção , Toxinas Marinhas , Microcistinas/metabolismo , Hibridização de Ácido Nucleico/efeitos dos fármacos , Poluentes Químicos da Água/análise , Poluentes Químicos da Água/metabolismoRESUMO
Okadaic acid (OKA), a marine toxin produced by dinoflagellates, is responsible for most human diarrhetic shellfish poisoning-associated health disorders. A competitive displacement assay for OKA is described here. An OKA-binding aptamer was truncated with two sequences, one labeled with 6-carboxyfluorescein (FAM), and one with a quencher. On addition of OKA, it will bind to the aptamer and green fluorescence pops up because label and quencher become spatially separated. One of the truncated aptamers exhibis an excellent binding capability (Kd 2.77 nM) for OKA compared to its full-length aptamer (526 nM). The selectivity of the assay was proven by the successful fluorometric determination of OKA in the presence of common diarrhoetic toxins and in shellfish extracts. The detection limit is as low as 39 pg·mL-1. Graphical abstract Schematic representation of the competitive displacement assay for okadaic acid (OKA). The OKA-binding aptamer is truncated with two parts, one labeled with 6-carboxyfluorescein (FAM), and one with a quencher. On addition of OKA, green fluorescence pops up because label and quencher become spatially separated.
Assuntos
Aptâmeros de Nucleotídeos/química , Fluoresceínas/química , Corantes Fluorescentes/química , Fluorometria/métodos , Toxinas Marinhas/análise , Ácido Okadáico/análise , Técnicas Biossensoriais/métodos , Misturas Complexas/química , Ouro/química , Limite de Detecção , Nanopartículas Metálicas/química , Frutos do Mar/análiseRESUMO
The autosomal recessive-hyper immunoglobulin E syndromes (AR-HIES) are inherited inborn primary immunodeficiency disorders caused mainly by mutations in the dedicator of cytokinesis 8 (DOCK8) gene. A method is described for the selection of DNA aptamers against DOCK8 protein. The selection was performed by using a gold electrode as the solid matrix for immobilization of DOCK8. This enables voltammetric monitoring of the bound DNA after each selection cycle. After eight rounds of selection, high affinity DNA aptamers for DOCK8 were identified with dissociation constants (Kds) ranging from 3.3 to 66 nM. The aptamer which a Kd of 8.8 nM was used in an aptasensor. A gold electrode was modified by self-assembly of the thiolated aptamer, and the response to the DOCK8 protein was detected by monitoring the change in the electron transfer resistance using the ferro/ferricyanide system as a redox probe. The aptasensor works in the 100 pg.mL-1 to 100 ng.mL-1 DOCK8 concentration range, has a detection limit of 81 pg.mL-1 and good selectivity over other proteins in the serum. Graphical abstractSchematic representation of an electrochemical screening protocol for the selection of DNA aptamer against dedicator of cytokinesis 8 protein using electrode as solid support for target immobilization.
Assuntos
Aptâmeros de Nucleotídeos/química , Fatores de Troca do Nucleotídeo Guanina/análise , Proteínas Imobilizadas/análise , Técnicas Biossensoriais , Espectroscopia Dielétrica , Dimerização , Técnicas Eletroquímicas/métodos , Eletrodos , Ferricianetos/química , Ouro/química , Limite de Detecção , Oxirredução , Técnica de Seleção de Aptâmeros/métodos , Sensibilidade e Especificidade , Compostos de Sulfidrila/química , Propriedades de SuperfícieRESUMO
Signal transduction based on fluorescence is one of the most common optical aptasensors for small molecules. Sensors with a number of unique features including high sensitivity, low cost, and simple operation can be constructed easily. However, the label-free fluorescent approach is limited to synthetic dyes that bind strongly to the aptamer sequence and result in a diminished sensor operation with high detection limits. In this study, we report the use of curcumin as a fluorescent probe to signal aptamer/small target binding events. A substantial enhancement in curcumin's fluorescent emission was observed when bound into the grooves of vitamin D3 (VTD3) binding aptamer, as an example. However, the introduction of the target molecule causes the aptamer to undergo a conformational change that favors complexing the target molecule over binding the curcumin dye. The sensor was able to detect VTD3 down to 1 fM concentration in buffer solutions and extracted blood samples, operate at a wide dynamic range, and discriminate against potential biological interfering molecules including VTD2. The operation of the curcumin based fluorescent sensor is at least six orders of magnitude more sensitive than a VTD3 sensor constructed with the synthetic dye SYBR Green I. The generality of the reported label-free approach was applied with a previously isolated 75-mer bisphenol-A (BPA) aptamer, confirming that the reported sensing strategy is not confined on a particular aptamer sequence. Our work not only reports a novel sensor format for the detection of small molecules, but also serves fluorescent sensor's most pressing need being novel fluorophores for multiplex targets detection.
RESUMO
The authors describe the identification of an effective binding region of aptamers against glycated (HbA1c) and total haemoglobin (tHb) by using a fluorometric assay. Truncation of the originally selected aptamers from 60 to 46 and 34 nucleotides for HbA1c and tHb, respectively, enhances the affinity for their targets. Moreover, shortening the aptamer sequences leads to a better conformational change after target binding which enabled the integration of the aptamers in a graphene oxide (GO)-based fluorometric assay. First, fluorescein-labelled truncated aptamers were physically absorbed onto the surface of GO surface via π-stacking interaction. This leads to quenching of fluorescence. Once the truncated aptamers bind the target protein, a conformational change is induced which results (a) )in the release of the aptamers from the surface of GO and (b) in the restoration of green fluorescence that is measured at 515 nm. The assay can be carried out in a microtiter plate format in homogeneous solution, this avoiding the steps of immobilization, incubation, and washing that are often necessary in immunoassays. This also reduces the time and the costs of the overall assay and allows for high throughput screening for diabetes. HbA1c can be detected in the range from 5.4 to 10.6%. The assay is selective for HbA1c over other proteins that commonly exist in blood. The results obtained by using this method compare well with those of a turbidimetric immunoassay that is typically applied in clinical laboratories. Graphical abstract Truncated aptamers for total and glycated hemoglobin were selected and integrated into a graphene oxide-based fluorescence detection assay for high-throughput screening for diabetes.