Impact of Overhead Evaporative Cooling, Canopy Location, Sunlight Exposure, Inoculation Level, Region, and Growing Season on the Survival of Generic Escherichia coli on in-Field Fuji Apples.

AIMS: The survival of inoculated Escherichia coli on Fuji apples in Washington State orchards was studied, considering evaporative cooling, canopy location, year, and region, with the examination of sunlight exposure and inoculation levels in year 2. METHODS AND RESULTS: Rifampicin-resistant E. coli was applied to Fuji apples. Initial concentrations for the high-inoculation study were 7.4 ± 0.3 log10 CFU per apple and 3.4 ± 0.3 log10 CFU per apple for the low-inoculation study. Enumeration of E. coli was conducted at 0, 2, 10, 18, 34, 42, 58, 82, 106, and 154 h after inoculation. Results were analyzed using Tukey's honest significance difference test and a log-linear model. Log-linear, Weibull, and Biphasic models characterized E. coli die-off patterns for high and low inoculations. The application of evaporative overhead cooling water did not significantly influence E. coli survival on Fuji apples; inoculation level and sunlight exposure were significant factors in a log-linear model. E. coli decreased by 5.5 ± 1.3 and 3.3 ± 0.4 log10 CFU per apple for high and low inoculated apples, respectively, by 154 h. The Biphasic model best explained the die-off pattern for high and low-inoculated Fuji apples. CONCLUSIONS: Overhead evaporative cooling, a useful fruit quality practice, did not impact the survival of generic E. coli on Fuji apple surfaces. The significant impact of sunlight exposure and inoculation levels on die-off highlights the importance of ultraviolet radiation in risk reduction and the need for various inoculum concentrations in preharvest field studies.

Dynamic changes in water resources and comprehensive assessment of water resource utilization efficiency in the Aral Sea basin, Central Asia.

The Aral Sea Basin in Central Asia faces significant challenges in improving water utilization and treatment because of frequent transboundary river water disputes and shortages of water resources. However, the traditional water resource utilization efficiency (WRUE) assessment models generally have the defect of over-validating evaluation results. To solve this problem, this study used the Coefficient of Variation method to constrain the self-contained weights in the traditional Data Envelopment Analysis (DEA) to construct an improved CV-DEA model, and assessed the WRUE of the Aral Sea Basin countries during 2000-2018 and compared the WRUE with that of the countries in the Mekong River Basin and Northeast Asia, then explored the factors influencing water utilization. The conclusions were drawn: since 1960, the runoff from the upper Amu Darya and Syr Darya rivers increased significantly, while the runoff from the lower Amu Darya River into the Aral Sea declined. Meanwhile, the water area of the Aral Sea shrank from 2.56 × 104 km2 to 0.70 × 104 km2 in 2000-2018, with the Northern Aral Sea remaining stable while the southern part shrinking sharply. The WRUE of the Aral Sea Basin (0.599, on average) was higher than that of the Mekong River Basin (0.547) and lower than that of Northeast Asia (0.885). Kazakhstan and Uzbekistan had the highest WRUE of 0.819 and 0.685 respectively, and the WRUE in both two countries improved from 2000 to 2018. Tajikistan (0.495) and Turkmenistan (0.402) experienced decreases in WRUEs. The high input redundancy of agricultural water consumption was the main driving force affecting WRUE in the basin.

Addressing the C/N imbalance in the treatment of irrigated agricultural water by using a hybrid constructed wetland at field-scale.

To mitigate excess of nitrate-N (NO3--N) derived from agricultural activity, constructed wetlands (CWs) are created to simulate natural removal mechanisms. Irrigated agricultural drainage water is commonly characterized by an organic carbon/nitrogen (C/N) imbalance, thus, C limitation constrains heterotrophic denitrification, the main biotic process implicated in NO3--N removal in wetlands. We studied a pilot plant with three series (169 m2) of hybrid CWs over the first two years of functioning to examine: i) the effect of adding different C-rich substrates (natural soil vs. biochar) to gravel on NO3--N removal in a subsurface flow (Phase I), ii) the role of a second phase with a horizontal surface flow (Phase II) as a source of dissolved organic C (DOC), and its effect in a consecutive horizontal subsurface flow (Phase III) on NO3--N removal, and iii) the contribution of each phase to global NO3--N removal. Our results showed that the addition of a C-rich substrate to gravel had a positive effect on NO3--N removal in Phase I, with mean efficiencies of 40% and 17% for soil and biochar addition, respectively, compared to only gravel (0.75%). In Phase II, the algae growth turned into a DOC concentration increase, but it did not enhance NO3--N removal in Phase III. In series with C-rich substrate addition, the largest contribution to NO3--N removal was found in Phase I. However, in series with only gravel, Phase II was the most effective on NO3--N removal. Contribution of Phase III to NO3--N removal was almost negligible.

Impact of irrigation water type and sampling frequency on Microbial Water Quality Profiles required for compliance with U.S. Food Safety Modernization Act Produce Safety Rule standards.

The U.S. Food Safety Modernization Act (FSMA) Produce Safety Rule (PSR) requires that farmers generate a Microbial Water Quality Profile (MWQP) from 20 samples per agricultural water source, taken over 2-4 years and five annual samples thereafter. Farmers must use the MWQP to ascertain a geometric mean (GM) of ≤126 CFU/100 mL and statistical threshold value (STV) of ≤410 CFU/100 mL of generic Escherichia coli. Farmers are responsible for collecting samples and paying for testing, incurring a financial and time burden. To determine if testing frequency can be reduced without compromising accuracy, water samples (n = 279) were collected from twelve sites in the U.S. Mid-Atlantic region from 2016 to 2018 comprising tidal brackish river, non-tidal fresh river, pond, vegetable processing, and reclaimed water. The GM and STV were calculated for all sites and water types using all samples, and for multiple sub-samples of <20 from each site and water type. A Monte Carlo simulation was used to determine the proportion of sub-sample sizes that yielded the same determination as the entire sample size of PSR standard compliance. Four sites, two pond and two reclaimed water sites, complied with PSR GM and STV requirements when using the entire sample set. When a water source's calculated GM and STV using the entire sample set hovered close to the PSR thresholds, sub-sample sizes approached the recommended 20 samples to reach a congruent compliance determination. However, 99% agreement was obtained with a sub-sample of five when the absolute difference between the GM and STV from total samples and the PSR thresholds was ≥2.6 and 4.5 log CFU/100 mL E. coli, respectively. These findings suggest that under certain conditions the MWQP may be generated with well below 20 samples, reducing the economic burden on farmers while still maintaining a representative MWQP.

Risk regulation of water allocation in irrigation areas under changing water supply and demand conditions.

The uncertainty of the hydrological environment and unbalanced water resource allocation result in a high risk of irrigation water shortages in regional agriculture, which seriously affects the sustainable development of agricultural systems. In this paper, we propose a risk regulation based modeling approach for the optimal allocation of agricultural water resources in a complex stochastic environment. The approach includes a conditional value-at-risk (CVaR) model, two-stage stochastic programming (TSP) model, two-dimensional joint distribution probability (JP) model, fractal criteria, and a multiple forms of chance-constrained programming (CCP) model. The model can weigh the contradiction between the intended target and associated penalties attributed to unknown hydrological events, measure the risk between system benefits and expected losses in agricultural water allocation at different confidence levels, and address the randomness in the objective function and constraints (including the left end term, right end term, and left and right end terms). To verify the applicability of the method, it is applied to the Jinxi Irrigation District in China to optimize the allocation and risk regulation of limited water resources under the variable runoff conditions of the Songhua River and crop water demands in the irrigation area. By adjusting parameters such as risk preference and probability of violation, the risk of water shortages in the irrigation area can be regulated, and the multidimensional impacts of different water allocation schemes on agricultural economic benefits, social benefits, ecology and environment can be determined. The case study reveals that the CTSP-CCJP method is sensitive, applicable to complex and uncertain environments and important for the efficient use of agricultural water resources and risk reduction.

An improved fuzzy sorting algorithm coupling bi-level programming for synergetic optimization of agricultural water resources: A case study of Fujian Province, China.

The uneven allocation of water resources and the shortage of regional water resources pose great challenges to the economic development and regional development balance of the Fujian province. Optimizing the water allocation structure in different regions can effectively alleviate water pressure. In this study, a type-2 fuzzy bi-level programming (T2FBL) method is proposed to plan the agricultural water resource system in the Fujian province. This method uses an improved fuzzy sorting algorithm to deal with uncertain parameters in the system and combines the bi-level programming method to balance the trade-off between two levels of decision-makers, the uncertain information contained in the secondary membership function omitted in the interval type-2 fuzzy theory is considered in the new ranking algorithm. Multiple scenarios related to different food security needs and different risk indices are examined. The major findings are as follows: (i) With an average tolerance of 75%, the average gross agricultural output value under various scenarios increased (0.4% âˆ¼ 7%) (average 3.89%) after optimization. (ii) The regional water allocation scheme under different food demands and different water availability scenarios is calculated, and the results show that prioritizing adjustments to the industrial water distribution structure of Fuzhou and Zhangzhou will greatly relieve the water pressure in the Fujian province. (iii) The relationship between the availability of system water resources and economic benefits is given through the calculation results of the T2FBL model. These findings can provide an in-depth understanding of the interaction between agricultural, industrial and tertiary industry water allocation and provide technical support for agricultural water resource planning issues.

Spatial-temporal variation and driving factors decomposition of agricultural grey water footprint in China.

The management of agricultural water pollution is crucial to alleviate the water crisis and promote regional sustainable development. Therefore, it is necessary to clarify the spatial-temporal variation characteristics of the agricultural grey water footprint (GWF) and accurately identify its main influencing factors, aiming at formulating differentiated regional management strategies. Based on this, the agricultural GWFs of 31 provincial regions in China from 2011 to 2019 were firstly calculated, and then the spatial-temporal variation characteristics of agricultural GWF were analyzed using the ArcGIS software and Standard Deviational Ellipse (SDE) method. Finally, the Generalized Divisia Index Method (GDIM) was creatively introduced to decompose the factors of agricultural GWF change and their respective contributions at the national and provincial levels. The main results are as follow: (1) Agricultural GWF in China decreased on the whole and showed significant provincial differences. Among them, the agricultural GWF of Henan Province was the largest while that of Shanghai City was the smallest. Compared with 2011, most provinces saw a decrease in agricultural GWF in 2019 while Yunnan, Tibet, Qinghai, Ningxia and Xinjiang Provinces achieved growth. (2) Areas with higher agricultural GDP generally had higher agricultural GWF. The spatial distribution of agricultural GWF and breeding GWF generally tended to be consistent, with the lower value in northwest and southeast of China and higher value in the northeast and southwest of China. Meanwhile, the mean center of SDE of agricultural GWF was located in Henan Province from 2011 to 2018, and shifted to Shaanxi Province in 2019, showing a slight northwest shift. (3) Agricultural GWF intensity and agricultural GDP had the largest restraining effect and driving effect on agricultural GWF growth, respectively. Additionally, China has achieved decoupling between agricultural GWF and agricultural GDP, reflecting that the patterns of agricultural production and consumption have become more sustainable. The findings of this study can provide important decision-making insights for agricultural water pollution management and industry adjustment.

Parsimonious Mechanistic Modeling of Bacterial Runoff into Irrigation Ponds To Inform Food Safety Management of Agricultural Water Quality.

Pond irrigation water comprises a major pathway of pathogenic bacteria to fresh produce. Current regulatory methods have been shown to be ineffective in assessing this risk when variability of bacterial concentrations is large. This paper proposes using mechanistic modeling of bacterial transport as a way to identify improved strategies for mitigating this risk pathway. If the mechanistic model is successfully tested against observed data, global sensitivity analysis (GSA) can identify important mechanisms to inform alternative, preventive bacterial control practices. Model development favored parsimony and prediction of peak bacterial concentration events. Data from two highly variable surface water irrigation ponds showed that the model performance was similar or superior to that of existing pathogen transport models, with a Nash-Sutcliffe efficiency of 0.48 and 0.18 for the two ponds. GSA quantified bacterial sourcing and hydrology as the most important processes driving pond bacterial contamination events. Model analysis has two main implications for improved regulatory methods: that peak concentration events are associated with runoff-producing rainfall events and that intercepting bacterial runoff transport may be the best option to prevent bacterial contamination of surface water irrigation ponds and thus fresh produce. This research suggests the need for temporal management strategies. IMPORTANCE Preventive management of agricultural waters requires understanding of the drivers of bacterial contamination events. We propose mechanistic modeling as a way forward to understand and predict such events and have developed and tested a parsimonious model for rain-driven surface runoff contributing to generic Escherichia coli contamination of irrigation ponds in Central Florida. While the model was able to predict the timing of peak events reasonably well, the highly variable magnitude of the peaks was less well predicted. This indicates the need to collect more data on the fecal contamination inputs of these ponds and the use of mechanistic modeling and global sensitivity analysis to identify the most important data needs.

Microbial and physicochemical assessment of irrigation water treatment methods.

AIMS: The presence of foodborne pathogens in preharvest agricultural water has been identified as a potential contamination source in outbreak investigations, driving markets and auditing bodies to begin requiring water treatment for high-risk produce. Therefore, it is essential that we identify water treatment methods which are effective as well as practical in their application on farm. METHODS AND RESULTS: In this work, we evaluated two sanitizers which are most prominent in preharvest agricultural water treatment (calcium hypochlorite (free chlorine: 3-5 ppm) and peracetic acid (PAA: 5 ppm)), an EPA registered antimicrobial device (ultraviolet light (UV)), in addition to a combination approach (chlorine + UV, PAA + UV). Treatments were evaluated for their ability to inactivate total coliforms and generic Escherichia coli and consistency in treatment efficacy over 1 h of operation. Physicochemical variables were measured along with microbial populations at 0, 5, 15, 30, 45 and 60 min of operation. Escherichia coli and coliform counts showed a significant (P < 0·05) reduction after treatment, with combination and singular treatments equally effective at inactivating E. coli and coliforms. A significant increase (P < 0·05) in oxidation-reduction potential was seen during water treatment (Chlorine; UV + Chlorine), and a significant reduction (P < 0·05) in pH was seen after PAA and PAA + UV treatments (60 min). CONCLUSION: Overall, the results indicate that all treatments evaluated are equally efficacious for inactivating E. coli and coliforms present in surface agricultural water. SIGNIFICANCE AND IMPACT OF THE STUDY: This information when paired with challenge studies targeting foodborne pathogens of interest can be used to support grower decisions when selecting and validating a preharvest agricultural water treatment programme.

Accurate Spectral Estimation Technique Based on Decimated Linear Predictor for Leak Detection in Waterworks.

Rural pipelines dedicated to water distribution, that is, waterworks, are essential for agriculture, notably plantations and greenhouse cultivation. Water is a primary resource for agriculture, and its optimized management is a key aspect. Saving water dispersion is not only an economic problem but also an environmental one. Spectral estimation of leakage is based on processing signals captured from sensors and/or transducers generally mounted on pipelines. There are different techniques capable of processing signals and displaying the actual position of leaks. Not all algorithms are suitable for all signals. That means, for pipelines located underground, for example, external vibrations affect the spectral response quality; then, depending on external vibrations/noises and flow velocity within pipeline, one should choose a suitable algorithm that fits better with the expected results in terms of leak position on the pipeline and expected time for localizing the leak. This paper presents findings related to the application of a decimated linear prediction (DLP) algorithm for agriculture and rural environments. In a certain manner, the application also detects the hydrodynamics of the water transportation. A general statement on the issue, DLP illustration, a real application and results are also included.

Salinity, freshwater and agricultural water preferences of glass eels of the Japanese eel Anguilla japonica collected in southern Japan.

Water-choice trial experiments revealed that Anguilla japonica glass eels collected in southern Japan possess strong preferences for fresh water and agricultural water. Their locomotor activity and preference for fresh water were higher and stronger, respectively, in this study when compared to previous studies conducted at lower temperatures. These results suggest that their locomotor activity and preference for fresh water is influenced by water temperature. The attraction to agricultural water indicates their upstream migration and habitat selection could be influenced by agricultural water.

Potential risk assessment of groundwater to address the agricultural and domestic challenges in Ordos Basin.

The safety of groundwater has been a great concern for irrigation and drinking purposes in recent decades due to the increasing impacts of anthropogenic activities. There are several standards to evaluate the groundwater quality for different utilization purposes. In this paper, 804 samples covering the entire Ordos Basin across five provinces were used to evaluate the irrigation suitability and human health risks. The results showed that the sequence of cationic concentration was Na+ > Ca2+ > Mg2+ > K+ > NH4 +, and the anion concentration was HCO3 - > SO4 2- > Cl- > NO3 - > F- > NO2 -. For drinking purposes, TDS, Na+, SO4 2-, F-, TH and NO3 - exceed seriously the regulated standard in the study area. For irrigating purposes, 80% of the water in the study area belongs to "good water" according to the evaluation of salinity and alkalinity. Saline water which is not suitable for irrigation accounts for about 9%. High health risks of fluoride ions are mainly observed to the samples representing the western part of the study area, while the health risks of nitrates spread throughout the study area. Health risk is not only related to location, but also varies with age, and it is found that children suffer more threats than adults.

Achieving sustainable soil and water protection: The perspective of agricultural water price regulation on environmental protection.

With the development of Chinese economy, more and more attention has been paid to environmental protection, the implementation of water price policy affects economic and environmental changes in China. This paper analyzes the impact of water price policy on agricultural land use and the scale of water pollution discharge in 240 cities in China between 2001 and 2017, by including data from China Urban Statistical Yearbook and China Land & Resources Almanac. The theoretical analysis of this study indicates that the optimal scale of pollution depends on the local initial endowment, economic investment capital and the marginal cost of environmental pollution caused by government's economic activities. Furtherly, the economic activities have a worsening impact on environmental pollution, but when the government implements environmental protection and water price policy measures in response to environmental pollution caused by economic activities, it has a significant impact on the decline in the scale of pollution. The government has promoted the pollution suppression model in the formulation of water prices, which has internalized the external cost of pollution in economic activities and can effectively reduce the scale of agricultural water pollution discharge.

Dead-End Ultrafiltration and DNA-Based Methods for Detection of Cyclospora cayetanensis in Agricultural Water.

Cyclospora cayetanensis is a protozoan parasite that causes foodborne and waterborne diarrheal illness outbreaks worldwide. Most of these outbreaks are associated with the consumption of fresh produce. Sensitive and specific methods to detect C. cayetanensis in agricultural water are needed to identify the parasite in agricultural water used to irrigate crops that have been implicated in outbreaks. In this study, a method to detect C. cayetanensis in water by combining dead-end ultrafiltration (DEUF) with sensitive and specific molecular detection was developed and evaluated. Triplicates of 10-liter agricultural water samples were seeded with 200, 100, 25, 12, and 6 C. cayetanensis oocysts. Surface water samples were also collected in the Mid-Atlantic region. All water samples were processed by DEUF and backflushed from the ultrafilters. DNA was extracted from concentrated samples and analyzed by quantitative PCR (qPCR) targeting the C. cayetanensis 18S rRNA gene. All water samples seeded with 12, 25, 100, and 200 oocysts were positive, and all unseeded samples were negative. Samples seeded with 6 oocysts had a detection rate of 66.6% (8/12). The method was also able to detect C. cayetanensis isolates in surface water samples from different locations of the Chesapeake and Ohio Canal (C&O Canal) in Maryland. This approach could consistently detect C. cayetanensis DNA in 10-liter agricultural water samples contaminated with low levels of oocysts, equivalent to the levels that may be found in naturally incurred environmental water sources. Our data demonstrate the robustness of the method as a useful tool to detect C. cayetanensis from environmental sources.IMPORTANCECyclospora cayetanensis is a protozoan parasite that causes foodborne and waterborne outbreaks of diarrheal illness worldwide. These foodborne outbreaks associated with the consumption of fresh produce and agricultural water could play a role in the contamination process. In this study, a method to detect C. cayetanensis in agricultural water by combining a robust filtration system with sensitive and specific molecular detection was developed and validated by the FDA. The results showed that this approach could consistently detect low levels of C. cayetanensis contamination in 10 liters of agricultural water, corresponding to the levels that may be found in naturally occurring environmental water sources. The method was also able to detect C. cayetanensis in surface water samples from a specific location in the Mid-Atlantic region. Our data demonstrate the robustness of the method to detect C. cayetanensis in agricultural water samples, which could be very useful to identify environmental sources of contamination.

Decoupling of forest water supply and agricultural water demand attributable to deforestation in North Korea.

This study focused on changes in water balance attributable to severe deforestation in North Korea. The forest water supply and agricultural water demand of North Korea were quantified to identify their decoupling over the past three decades. Forest water supply and agricultural water demand before and after deforestation were estimated using the InVEST-WY (Integrated Valuation of Ecosystem Services and Trade-offs - Water Yield) and EPIC (Environmental Policy Integrated Climate) models, respectively. Analysis of land cover change before and after deforestation showed that area under forests decreased by 25%, whereas that under cropland increased by 63%, and that the conversion from forest to cropland was the largest for the study period. As a result, agricultural water demand increased and forest water supply decreased, significantly. Analysis of the net impact of deforestation on water budgets using recent climate and two land covers showed that forest water supply decreased by 43% and agricultural water demand increased by 62%. An assessment of the water balance at the watershed level showed that the Taedong, Ryesong, and Tumen Rivers suffered the largest negative change in terms of the large gross impact of deforestation on water resources. The water balance of the entire North Korea has declined by 51% and this is attributable to deforestation. In contrast, South Korea has experienced success in national-scale afforestation in recent decades, and North Korea can emulate this. The restoration of forests in North Korea promises more than environmental benefits; it will provide a new growth engine for the prosperity of the Korean Peninsula as a whole.

Meeting Report: Key Outcomes from a Collaborative Summit on Agricultural Water Standards for Fresh Produce.

On February 27 to 28, 2018, the Produce Safety Alliance convened a national water summit in Covington, KY to discuss the requirements of the United States Food and Drug Administration's (FDA) Food Safety Modernization Act Standards for the Growing, Harvesting, Packing, and Holding of Produce for Human Consumption (Produce Safety Rule [PSR]). The goals of the meeting were to better understand the challenges growers face in implementing the requirements in Subpart E-Agricultural Water and work collaboratively to develop practical solutions to meet fruit and vegetable production needs while protecting public health. To meet these goals, the summit engaged a diverse group of stakeholders including growers, researchers, extension educators, produce industry members, and regulatory personnel. Key outcomes included defining implementation barriers due to diversity in water sources, distribution systems, commodity types, climates, farm size, and production activities. There was an articulated need for science-based solutions, such as the use of agricultural water system assessments and sharing of federal, state, and regional water quality data, to ensure qualitative and quantitative standards reduce microbial risks. These identified challenges and needs resulted in significant debate about whether reopening the PSR-Subpart E for modification or attempting to address concerns through guidance would provide the best mechanism for alleviating concerns. In addition, training, outreach, and technical assistance were identified as vital priorities once the concerns are formally addressed by FDA. The water summit highlighted the critical need for transparency of FDA's progress on reevaluating the Subpart E requirements to help guide growers' decisions regarding the use of agricultural water.

Agricultural Water Resources Management Using Maximum Entropy and Entropy-Weight-Based TOPSIS Methods.

Allocation and management of agricultural water resources is an emerging concern due to diminishing water supplies and increasing water demands. To achieve economic, social, and environmental goals in a specific irrigation district, decisions should be made subject to the changing water supply and water demand-the two critical random parameters in agricultural water resources management. This paper presents the foundations of a systematic framework for agricultural water resources management, including determination of distribution functions, joint probability of water supply and water demand, optimal allocation of agricultural water resources, and evaluation of various schemes according to agricultural water resources carrying capacity. The maximum entropy method is used to estimate parameters of probability distributions of water supply and demand, which is the basic for the other parts of the framework. The entropy-weight-based TOPSIS method is applied to evaluate agricultural water resources allocation schemes, because it avoids the subjectivity of weight determination and reflects the dynamic changing trend of agricultural water resources carrying capacity. A case study using an irrigation district in Northeast China is used to demonstrate the feasibility and applicability of the framework. It is found that the framework works effectively to balance multiple objectives and provides alternative schemes, considering the combinatorial variety of water supply and water demand, which are conducive to agricultural water resources planning.

Disinfection by-products in baby lettuce irrigated with electrolysed water.

BACKGROUND: Irrigation water disinfection reduces the microbial load but it might lead to the formation and accumulation of disinfection by-products (DBPs) in the crop. If DBPs are present in the irrigation water, they can accumulate in the crop, particularly after the regrowth, and be affected by the postharvest handling such as washing and storage. To evaluate the potential accumulation of DBPs, baby lettuce was grown using irrigation water treated with electrolysed water (EW) in a commercial greenhouse over three consecutive harvests and regrowths. The impact of postharvest practices such as washing and storage on DBP content was also assessed. RESULTS: Use of EW caused the accumulation of chlorates in irrigation water (0.02-0.14 mg L-1 ), and in the fresh produce (0.05-0.10 mg kg-1 ). On the other hand, the disinfection treatment had minor impact regarding the presence of trihalomethanes (THMs) in water (0.3-8.7 µg L-1 max), and in baby lettuce (0.3-2.9 µg kg-1 max). CONCLUSIONS: Disinfection of irrigation water with EW caused the accumulation of chlorates in the crop reaching levels higher than the current maximum residual limit established in the EU legislation for leafy greens. © 2017 Society of Chemical Industry.

Application of chitosan microparticles for mitigation of Salmonella in agricultural water.

AIM: The activity of chitosan microparticles (CM) was examined using a matrix of conditions in order to assess the efficacy of CM as a mitigation against various strains of Salmonella enterica in agricultural water. METHODS AND RESULTS: Different concentrations of CM (0, 0·01, 0·1, 0·2, 0·3% w/v) were examined for antimicrobial activity against log vs stationary phase cells of Salmonella and at different conditions of temperature, salinity and pH. Results showed greatest activity with 0·3% CM at pH 7, 25-37°C without additional of salt. Significant reductions in Salmonella levels were also achieved in natural pond water, although decreases were reduced compared to sterile water. All serotypes were sensitive to CM, with minimal inhibitory concentrations ranging from 0·0031 to 0·0250% w/v. Phylogenic analysis of Javiana strains showed increased resistance appeared in multiple genetic lineages. CONCLUSION: Conditions demonstrating greatest CM activity were compatible with agricultural practices. Although sensitivity to CM varied among Salmonella strains, all strains were sensitive under conditions examined in this study. SIGNIFICANCE AND IMPACT OF THE STUDY: This research indicated that CM, a natural compound with minimal environmental impact, could be an effective alternative for mitigating Salmonella in agricultural water applications.

Agricultural water policy reforms in China: a representative look at Zhangye City, Gansu Province, China.

Water resources are essential for agricultural production in the grain-producing region of China, and water shortage could significantly affect the production and international trade of agricultural products. China is placing effort in new policies to effectively respond to changes in water resources due to changes in land use/land cover as well as climatic variations. This research investigates the changes in land, water, and the awareness of farmer vis-à-vis the implementation of water-saving policies in Zhangye City, an experimental site for pilot programs of water resources management in China. This research indicates that the water saved through water-saving programs and changes in cropping structure (2.2 × 108 m3 a-1) is perhaps lower than the newly increased water withdrawal through corporate-led land reclamation (3.7 × 108 m3 a-1). Most critically, the groundwater withdrawal has increased. In addition, our survey suggests that local government is facing a dilemma of water conservation and agricultural development. Therefore, the enforcement of the ban on farmland reclamation and irrigation water quotas in our study area is revealed to be relatively loose. In this vein, the engagement of local stakeholders in water governance is essential for the future sustainable management of water resources.