Variations in amino acids caused by drought stress mediate the predisposition of Carya cathayensis to Botryosphaeria canker disease.

Abiotic stresses can affect the outcome of plant-pathogen interactions, mostly by predisposing the host plant to infection; however, the crosstalk between pathogens and plants related to such predisposition remains unclear. Here, we investigated the predisposition of Carya cathayensis to infection by the fungal pathogen Botryosphaeria dothidea (Bd) caused by drought in the host plant. High levels of drought stress resulted in a significant increase in plant susceptibility to Bd. Drought significantly induced the accumulation of H2O2 and the free amino acids Pro, Leu, and Ile, and in the phloem tissues of plants, and decreased the content of non-structural carbohydrates. In vitro assays showed that Bd was sensitive to H2O2; however, Pro played a protective role against exogenous H2O2. Leu, Ile, and Pro induced asexual reproduction of Bd. Our results provide the first analysis of how drought predisposes C. cathayensis to Botrysphaeria canker via amino acid accumulation in the host plant, and we propose a model that integrates the plant-pathogen interactions involved.

In vivo effect of deoxynivalenol (DON) naturally contaminated feed on porcine reproductive and respiratory syndrome virus (PRRSV) infection.

Deoxynivalenol (DON), also known as vomitoxin, is the most prevalent type B trichothecene mycotoxin worldwide. Pigs show a great sensitivity to DON, and because of the high proportion of grains in their diets, they are frequently exposed to this mycotoxin. The objective of this study was to determine the impact of DON naturally contaminated feed on porcine reproductive and respiratory syndrome virus (PRRSV) infection, the most important porcine viral pathogen in swine. Experimental infections were performed with 30 animals. Piglets were randomly divided into three groups of 10 animals based on DON content of diets (0, 2.5 and 3.5 mg/kg DON). All experimental groups were further divided into subgroups of 6 pigs and were inoculated with PRRSV. The remaining pigs (control) were sham-inoculated with PBS. Pigs were daily monitored for temperature, weight and clinical signs for 21 days. Blood samples were collected and tested for PRRSV RNA and for virus specific antibodies. Results of PRRSV infection showed that ingestion of diet highly contaminated with DON greatly increases the effect of PRRSV infection on weight gain, lung lesions and mortality, without increasing significantly viral replication, for which the tendency is rather directed toward a decrease of replication. These results suggest that PRRSV infection could exacerbate anorectic effect of DON, when ingested in large doses. Results also demonstrate a DON negative effect on PRRSV-specific humoral responses. This study demonstrate that high concentrations of DON naturally contaminated feed decreased the immune response against PRRSV and influenced the course of PRRSV infection in pigs.