Removal of toluene vapor in the absence and presence of a quorum-sensing molecule in a biotrickling filter and microbial composition shift.

Toluene is highly toxic and mutagenic, and it is generally used as an industrial solvent. Thus, toluene removal from air is necessary. To solve the problem of reducing high toluene concentrations with a short gas retention time (GRT), a quorum-sensing molecule [N-(3-oxododecanoyl)-L-homoserine lactone] (OHL) was added to a biotrickling filter (BTF). In this study, a BTF was used to treat synthetic and natural waste gases containing toluene. An extensive analysis was performed to understand the removal efficiency, removal characteristics, and bacterial community of the BTF. The addition of 20 µM OHL to the BTF significantly improved toluene removal, and more than 99.2% toluene removal was achieved at a GRT of 0.5 min when natural waste gas containing toluene (590-1020 ppm or 2.21-3.83 g m-3) was introduced. The maximum inlet load for toluene was 337.9 g m-3 h-1. Moreover, the BTF exhibited satisfactory adaptability to shock loading and shutdown operations. Pseudomonadaceae (33.0%) and Comamonadaceae (26.3%) were predominant bacteria in the system after a 98-day operation. These bacteria were responsible for toluene degradation. The optimal moisture content and low pressure drop for system operations demonstrated that the BTF was energy and cost efficient. Therefore, processing through a BTF with OHL is a favorable technique for toluene treatment.

Novel zero-valent iron-assembled reactor for strengthening anammox performance under low temperature.

To further expand the application of anammox biotechnology, a novel zero-valent iron-assembled upflow anaerobic sludge bed reactor was employed to strengthen anammox performance under low temperature and shock load. Packed with sponge iron and polyester sponge, this novel reactor could speed up the recovery of anammox activity in 12 days and improve the adaptability of anammox bacteria at the temperature of 10-15 °C. The high nitrogen loading rate of 1109.2 mg N/L/day could be adapted in 27 days and the new nitrogen pathway under the effect of sponge iron was clarified by batch experiment. Moreover, the real-time quantitative PCR analysis and Illumina MiSeq sequencing verified the dominant status of Candidatus Kuenenia stuttgartiensis and planctomycete KSU-1, as well as demonstrated the positive role of sponge iron on anammox microorganisms' proliferation. The findings might be beneficial to popularize anammox-related processes in municipal and industrial wastewater engineering.

Seasonal tourism's impact on wastewater composition: Evaluating the potential of alternating activated adsorption in primary treatment.

Primary treatment processes have gained attention in recent research and development due to their potential for redirecting carbon towards anaerobic digestion, which can subsequently be used for the production of biomethane. The alternating activated adsorption (AAA) process is implemented on full-scale at several wastewater treatment plants across Europe. However, there is a lack of full-scale studies of advanced carbon capture technology implementations in literature. This study demonstrates the ability of a full-scale AAA process to remove and redirect carbon in a region heavily influenced by tourism. Periods in high and off-season were compared to study the impact of tourism on the composition of the wastewater and the AAA-process. The wastewater characteristics of the high season differed significantly from the low season. During the high season, the PE increased by 37 %, total suspended solids went up by 75 % and chemical oxygen demand increased by 58 %, compared to the low season. Additionally, 80 % of the low volatile lipophilic substances (LVLS) measured were attributed to the impact of tourism. A mass-balance of primary treatment for chemical oxygen demand (COD) and LVLS was conducted for both trial periods. The primary treatment was able to eliminate 56 % of the COD and 62 % of the LVLS in the non-tourist season and 53 % of the COD and 54 % of the LVLS in the tourist season. The increased wastewater load was effectively managed in the AAA-process. Key process parameters like sludge settling characteristics, hydraulic retention time and total suspended solids removal rates remained stable during the high season in winter.

Continuous augmentation of anaerobic digestion with electroactive microorganisms: Performance and stability.

The performance and stability of a bioelectrochemical anaerobic digester (BeAD), continuously augmented with electroactive microorganisms (EAMs), were investigated. The BeAD showcased superior performance, sustaining the high COD removal efficiency and methane production rate of 76.5 % and 0.67 L/(L.d), respectively, in a stable state. Prominently, it exhibited remarkable resilience under hydraulic and organic shock loads, adeptly recuperating from disturbances up to 1000 % of its stable condition. This resilience of up to 300 % shock load was driven by increased levels of electron transport components such as quinones and riboflavins, which act as electron shuttles. However, after extreme shock exposures from 500 % to 1000 %, despite the spike in inhibitory by-products such as humic acids and ammonia, the upregulation of the mtr complex was pivotal in recovering and sustaining methane production. These insights emphasize the BeAD's capability to bolster both performance and stability, thereby providing a potent strategy for practical application of bioelectrochemical systems.

A novel design of journal bearings for stability under shock loads.

Mechanical systems are expected to operate under various load conditions, and it is necessary to use a lubrication system to achieve reliability and stable performance. Journal bearings, which are used to achieve such stable lubrication, are representative of hydrodynamic lubrication bearings. In this study, groove-shaped structures and rubber were applied to the ends of the bearings to ensure stable lubrication performance under conditions where, for various reasons, shock loads are applied in addition to static loads under misaligned conditions. The groove structure and rubber contribute to stable lubrication performance by preventing contact between the shaft and bearing as well as absorbing shock loads through elastic deformation of the groove's end due to oil film pressure. This novel design, which utilizes groove-type flexible structures and rubber, led to journal bearings that exhibited improved lubrication performance under various shock load conditions. When a shock load is applied to a mechanical system, the design proposed in this study contributes to improving the reliability of the mechanical system by enhancing its lubrication performance.

Response to shock load of titanium dioxide nanoparticles on aerobic granular sludge and algal-bacterial granular sludge processes.

Titanium dioxide nanoparticles (TiO2 NPs) are extensively used in various fields and can consequently be detected in wastewater, making it necessary to study their potential impacts on biological wastewater treatment processes. In this study, the shock-load impacts of TiO2 NPs were investigated at concentrations ranging between 1 and 200 mg L-1 on nutrient removal, extracellular polymeric substances (EPSs), microbial activity in aerobic granular sludge (AGS), and algal-bacterial granular sludge (AB-AGS) bioreactors. The results indicated that low concentration (≤10 mg L-1) TiO2 NPs had no effect on microbial activity or the removal of chemical oxygen demand (COD), nitrogen, and phosphorus, due to the increased production of extracellular polymeric substances (EPSs) in the sludge. In contrast, the performance of both AGS and AB-AGS bioreactors gradually deteriorated as the concentration of TiO2 NPs in the influent increased to 50, 100, and 200 mg L-1. Specifically, the ammonia­nitrogen removal rate in AGS decreased from 99.9 % to 88.6 %, while in AB-AGS it dropped to 91.3 % at 200 mg L-1 TiO2 NPs. Furthermore, the nitrate­nitrogen levels remained stable in AB-AGS, while NO3-N was detected in the effluent of AGS at 100 and 200 mg L-1. Microbial activities change similarly as smaller decrease in the specific ammonia uptake rate (SAUR) and specific nitrate uptake rate (SNUR) was found in AB-AGS compared to those in AGS. Overall, the algal-bacterial sludge exhibited higher resilience against TiO2 NPs, which was attributed to a) higher EPS volume, b) smaller decrease in LB-EPS, and c) the favorable protein to polysaccharide (PN/PS) ratio. This in turn, along with the symbiotic relationship between the algae and bacteria, mitigates the toxic effects of nanoparticles.

Effects of sudden shock load on simultaneous biohythane production in two-stage anerobic digestion of high-strength organic wastewater.

The two-stage anaerobic digestion (AD) for biohythane production is a sustainable solution, but it is sensitive to organic shock load that disrupts reactors and inhibits biohythane production. This study investigated biohythane production, reactor performance, and the possibility of post-failure restoration in a two-stage AD system designed for treating high-strength organic wastewater. Sudden shock load was applied by increasing the OLR threefold higher after reaching steady state phase. During shock load phase, hydrogen content, hydrogen yield and methane production rate (MPR) reached its peak values of 62.61 %, 1.641 mol H2/mol glucose, and 1.003 L CH4/L⋅d respectively before declining significantly. Interestingly, during the restorative phase, hydrogen production sharply declined to nearly zero, while methane production exhibited a resilience and reached its peak methane content of 52.2 %. The study successfully demonstrated the system's resilience to sudden shock load, ensuring stable methane production, while hydrogen production did not exhibit the same capability.

Effect of transient organic load and aeration changes to pollutant removal and extracellular polymeric substances.

Transient organic load shocks have an important influence on the removal of pollutants and the content and composition of extracellular polymeric substances (EPS). This study was based on a micro-pressure reactor (MPR) with the influent COD concentration as the variable, while different operating conditions were controlled by adjusting the aeration rate. The effect of single-cycle transient organic loading shocks on EPS and pollutant removal and the correlation between their changes were investigated. The results showed that COD removal was unaffected under the shock, and the effect of nitrogen and phosphorus removal decreased. As the incoming carbon source increased, the EPS content at shock increased, with the polysaccharide (PS) content being the most affected. As aeration increased, the effect of organic load shock on EPS and pollutant removal decreased. Under different aeration conditions, PS contributed to denitrification and anaerobic phosphorus release during transient organic load shocks, and protein (PN) contributed to aerobic phosphorus uptake. The reduction in PS and PN relative to the pre-shock caused by the shock resulted in the EPS exhibiting a favourable effect on COD removal and an inhibitory effect on the effectiveness of nitrogen and phosphorus removal.

Characteristics of organic removal for supermarket wastewater treatment with an anaerobic baffled reactor and efficacy evaluation of changing HRT.

An anaerobic baffled reactor (ABR) is one of the useful wastewater treatment technologies, but the knowledge about its treatment performance for actual wastewater with load fluctuation is limited. The organic removal performance of an ABR for treating supermarket wastewater was evaluated. The ABR, which consisted of eight columns, was examined under four hydraulic retention time (HRT) conditions of 19.4, 12.9, 8.0, and 4.4 h. As a result, the unfiltered chemical oxygen demand (COD) removal efficiency was 80 (±8) % at an HRT of 19.4 h. When the HRT was shortened to 12.9 h, the average unfiltered COD removal efficiency decreased to 58 (±15) %. However, it showed buffering effect against high load inflow in the first column, indicating that it is useful as a pretreatment system under this condition. At an HRT of 4.4 h, the unfiltered COD removal efficiency decreased to 9%, indicating the system failed. The results of the microbial community structure analysis showed that the detection frequency of acidogenic bacteria decreased in proportion to the extension of residence time in the reactor. These results indicate that the ABR is useful for the treatment of supermarket wastewater with load fluctuations as a main treatment system at a HRT of 19.4 h and as a pretreatment system at a HRT of 12.9 h.

Mechanical Response of MEMS Suspended Inductors under Shock Using the Transfer Matrix Method.

MEMS suspended inductors are susceptible to deformation under external forces, which can lead to the degradation of their electrical properties. The mechanical response of the inductor to a shock load is usually solved by a numerical method, such as the finite element method (FEM). In this paper, the transfer matrix method of linear multibody system (MSTMM) is used to solve the problem. The natural frequencies and mode shapes of the system are obtained first, then the dynamic response by modal superposition. The time and position of the maximum displacement response and the maximum Von Mises stress are determined theoretically and independently of the shock. Furthermore, the effects of shock amplitude and frequency on the response are discussed. These MSTMM results agree well with those determined using the FEM. We achieved an accurate analysis of the mechanical behaviors of the MEMS inductor under shock load.

Organics and nutrients removal in vertical flow wetlands: loading fluctuation and alternative media.

Two wetland systems (conventional and structurally modified) were studied for the removal of organics and nutrients from municipal wastewater. Each system consisted of three vertical flow (VF) wetlands, which were filled with agricultural, construction waste materials and planted with Phragmites australis and Canna indica. The wetland units were operated under constant and consecutive shock hydraulic load (HL). Input nutrients and organics load across the wetland units ranged between 4.0-116.0 g N/m2d, 0.5-23.0 g P/m2d, 1.0-527.0 g biochemical oxygen demand (BOD)/m2d and 16.0-686.0 g chemical oxygen demand (COD)/m2d. Nitrification and organic carbon availability controlled nitrogen (N) removals in first and third stage VF wetlands, respectively, during constant load phase; organics removals were influenced by dissolved oxygen concentration of municipal wastewater. Second stage VF wetlands (of both systems) were inefficient in terms of COD removals during shock load periods, which were counter-balanced by first and third stages. First stage VF wetlands achieved higher N removal rates than following stages during shock load periods. Wetland maturation provided a buffer against substantial HL increment and sharp input load decrease in latter shock and recovery phases, respectively. Agricultural waste (sugarcane bagasse) provided carbon to support denitrification; construction materials (recycled brick and crushed mortar) removed phosphorus (P) from wastewater through adsorption. Coliform removal in VF wetlands was achieved through media filtration. Structurally modified system achieved higher removals than the conventional system. BOD, COD, total nitrogen and NH4-N removal percentage across two systems ranged between 76-79%, 59-63%, 73-77% and 90-95%, respectively. In general, this study enlightens potential application of appropriate waste materials for wastewater treatment.

The performance and microbial communities of an anaerobic membrane bioreactor for treating fluctuating 2-chlorophenol wastewater.

An anaerobic membrane bioreactor (AnMBR) was used to treat low to high (5-200 mg/L) concentrations of 2-chlorophenol (2-CP) wastewater. The AnMBR achieved high and stable chemical oxygen demand removal and 2-CP removal with an average value of 93.2% and 94.2% under long hydraulic retention times (HRTs, 48-96 h), respectively. 2-CP removal efficiency of 98.6 mg/L/d was achieved with 2-CP concentration of 200 mg/L, which was much higher than that of other anaerobic bioreactors. Furthermore, volatile fatty acids didn't accumulate under high 2-CP loading. Long HRTs significantly reduced the membrane fouling as the fouling rate (0.90 × 109-5.44 × 109 m-1h-1) was low. Spirochaetaceae and Methanosaeta were the dominant microbes responsible for dechlorination, methanogenesis, and shock resistance. All these results demonstrate that this AnMBR operated under long HRTs is good and robust for fluctuating chlorophenols wastewater treatment, which has high potential for treating fluctuating refractory organics wastewater with the low membrane fouling rate.

Acute effects of hexavalent chromium on the performance and microbial community of activated sludge in aerobiotic reactors.

This study investigated the acute effects of hexavalent chromium (Cr(VI)) shock load at 2.5, 6 and 25 mg/L on the performance and bacterial community structures in aerobiotic activated sludge reactors. The results showed that eight-day Cr(VI) toxicity made the removal rates of COD and NH3-N to obviously decrease in all reactors. Furthermore, the higher the Cr(VI) concentration was, the more severe the influence on the whole system would be. The effect of Cr(VI) on NH3-N removal was more serious than that on COD and the function of nitrification was harder to recover. The specific oxygen uptake rate (SOUR) values dropped by 73%, 68% and 31% at 2.5, 6 and 25 mg Cr(VI)/L, respectively. The data of SOUR showed that though the concentration of Cr(VI) was low, the whole respiratory activity of bacteria was much affected. The relative abundance change of genus between the initial stage and the last showed that the bacterial community structure changed significantly. Comparing with the initial stage, the phyla of Proteobacteria, Acidobacteria and Planctomycetes were markedly reduced at the end stage. The genera of Ferruginibacter, Coxiella and Rhodanobacter were also markedly reduced. Although the performance of activated sludge can be restored at the end, the whole respiratory activity of bacteria was still at a low level according to the data of SOUR.

Stability of continuous and fed batch sequential anaerobic-anoxic-aerobic moving bed bioreactor systems at phenol shock load application.

The stability of two sequential moving bed bioreactor systems operated in anaerobic-anoxic-aerobic continuous moving bed bioreactor (CMBR: R1-R2-R3) and semi-continuous fed batch moving bed bioreactor (FMBR: B1-B2-B3) modes was assessed for phenol shock load (PSL) applications in the presence of thiocyanate and ammonia. Both the systems were exposed to 3000 mg phenol/L (PSL-I) and 3500 mg phenol/L (PSL-II) for 3 days each from initial 2500 mg phenol/L without any intermediate concentration at 6 days HRT (hydraulic retention time). The effect of PSL-I on R1 was reversible within 10-12 days. At PSL-II, R1 required 2 days stop of feed for stability and resumed removal efficiency of phenol (15%) and COD (3%). R2 remained robust to sustain both PSLs and recovered within 15 days from peak influent concentrations of 1727 mg phenol/L (removal: 67%) and 324 mg SCN--/L (removal: 68-70%). In B1, effluent COD increased by 2%, though effluent phenol decreased by 3% than the pre-shock condition after PSL-I exposure. B2 acted similar to R2 when exposed to PSLs. The effect of PSL-I on R3 and B3 was negligible. However, at PSL-II R3 became vulnerable for nitrification, whereas phenol, COD and SCN- removal remained unaffected. In B3, PSL-II caused a decrease in phenol, SCN- and NH+4-N removal. In B3, stop of feed for 4 days also did not improve nitrification. The performance of the CMBR system was better than that of the FMBR system for organic shock load exposure in the presence of multiple pollutants.

Enhancement of organic matter degradation and methane gas production of anaerobic granular sludge by degasification of dissolved hydrogen gas.

A hollow fiber degassing membrane (DM) was applied to enhance organic matter degradation and methane gas production of anaerobic granular sludge process by reducing the dissolved hydrogen gas (D-H2) concentration in the liquid phase. DM was installed in the bench-scale anaerobic granular sludge reactors and D-H2 was removed through DM using a vacuum pump. Degasification improved the organic matter degradation efficiency to 79% while the efficiency was 62% without degasification at 12,000mgL-1 of the influent T-COD concentration. Measurement of D-H2 concentrations in the liquid phase confirmed that D-H2 was removed by degasification. Furthermore, the effect of acetate concentrations on the organic matter degradation efficiency was investigated. At acetate concentrations above 3gL-1, organic matter degradation deteriorated. Degasification enhanced the propionate and acetate degradation. These results suggest that degasification reduced D-H2 concentration and volatile fatty acids concentrations, prevented pH drop, and subsequent enhanced organic matter degradation.

Utilizing ion-exchange resin to improve recovery from organic shock-loading in an AnMBR treating sewage sludge.

The addition of ion-exchange resin in a two-phase continuous AnMBR system treating primary sludge at ambient temperature (20 °C) was investigated as a means to improve reactor recovery after organic shock-loading. Four commercially available anion-exchange resins were evaluated for their ability to sorb soluble organics, specifically volatile fatty acids (VFA), from AnMBR effluent. The strong-base resin, Purolite TANEX, was determined the best resin for deployment in the continuous AnMBR having achieved the greatest removal of soluble chemical oxygen demand (sCOD) (up to 36%) and acetic acid (up to 48%) in preliminary batch testing. Addition of 100 and 300 g/L TANEX in the AnMBR system improved effluent quality reducing effluent COD concentrations by 48 and 75%, respectively, under normal operating conditions. After shock-loading with 16,000 mg COD/L as acetic acid, reactor recovery in terms of methane production was 9-58% faster with the addition of TANEX than without, under controlled pH conditions (pH: 7.4). After shock-loading the system twice without the addition of TANEX it was found that recovery improved by 19% suggesting that acclimation of the microbial community also played a role in reactor recovery. Microbial community analysis using 16 S Illumina MiSeq sequencing confirmed changes in the microbial community did occur in response to shock-loading, with higher relative abundance of Methanoscarcina in the majority of post-shock-load microbial communities. The highest relative abundance of Methanoscarcina (51-58%) was seen during operating periods with the addition of TANEX resin, leading to the conclusion that addition of the TANEX resin benefited reactor recovery by serving as a temporary physio-chemical sink for the excess acetic acid, allowing the microbial community time to adjust to their new environmental conditions and become better able to process the higher levels of acetic acid associated with the organic shock.

Response of a continuous biomethanation process to transient organic shock loads under controlled and uncontrolled pH conditions.

The organic loading rate (OLR) is a critical factor that controls the treatment efficiency and biogas production in anaerobic digestion (AD). Therefore, organic shock loads may cause significant process imbalances accompanied by a drop in pH and acid accumulation or even failure. This study investigated the response of a continuous mesophilic anaerobic bioreactor to a series of transient organic shock loads of the substrate whey permeate, a high-strength organic wastewater from cheese making. The reactor was subjected to organic shock loads of increasing magnitude (a one-day pulse of elevated feed organic concentration) under controlled (near 7) and uncontrolled pH conditions at a fixed HRT of 10 days. The reactor was resilient to up to a shock load of up to 8.0 g SCOD/L·d under controlled pH conditions but failed to recover from the serious imbalance caused by a 3.0-g SCOD/L·d shock load, thus indicating the critical effect of pH on system resilience. The acidified reactor was not restored by interrupted feeding under the acidic conditions that were formed (pH ≤ 4.5) but was successfully restored after pH adjustment to 7. The reactor subsequently reverted to continuous mode without pH control and showed a performance comparable to the stable performance at the design OLR of 1.0 g SCOD/L·d. The bacterial community structure shifted dynamically in association with disturbances in the reactor conditions, whereas the archaeal community structure remained simple and less variable during the shock loading experiments. The structural shifts of the bacterial community were well correlated with the process performance changes, and performance recovery was generally accompanied by recovery of the bacterial community structure. The overall results suggest that the reactor pH, rather than simply acting as an accumulation of organic acids, had a crucial effect on the resilience and robustness of the microbial community and thus on the reactor performance under organic shock loads.

A decentralized wastewater treatment system using microbial fuel cell techniques and its response to a copper shock load.

Bench-scale decentralized wastewater treatment systems using microbial fuel cell (MFC) techniques were constructed for simultaneous removal of carbonaceous and nitrogenous pollutants and electricity production from wastewater. The MFC was operated in continuous mode and immobilized Paracoccus pantotrophus cells were added in the cathodic compartment to achieve simultaneous nitrification-denitrification. After 150-day operation, the MFC system could effectively remove >96% COD and 100% ammonium, with 60-80% total nitrogen removal and around 0.2V voltage production. The results of copper (Cu) shock load showed that although 125 mg L(-1) Cu (II) would deteriorate the effluent quality and completely inhibit the electricity production, the microbial populations restored their ability to treat wastewater and produce electricity after a period around 30 days. Community analysis by the 454 pyrosequencing technique showed that the microbial compositions were significantly different and decreased in diversity after the Cu shock load.