RESUMO
We report the dynamic spatial organization of Caulobacter crescentus RNase E (RNA degradosome) and ribosomal protein L1 (ribosome) using 3D single-particle tracking and superresolution microscopy. RNase E formed clusters along the central axis of the cell, while weak clusters of ribosomal protein L1 were deployed throughout the cytoplasm. These results contrast with RNase E and ribosome distribution in Escherichia coli, where RNase E colocalizes with the cytoplasmic membrane and ribosomes accumulate in polar nucleoid-free zones. For both RNase E and ribosomes in Caulobacter, we observed a decrease in confinement and clustering upon transcription inhibition and subsequent depletion of nascent RNA, suggesting that RNA substrate availability for processing, degradation, and translation facilitates confinement and clustering. Importantly, RNase E cluster positions correlated with the subcellular location of chromosomal loci of two highly transcribed rRNA genes, suggesting that RNase E's function in rRNA processing occurs at the site of rRNA synthesis. Thus, components of the RNA degradosome and ribosome assembly are spatiotemporally organized in Caulobacter, with chromosomal readout serving as the template for this organization.
Assuntos
Proteínas de Bactérias/metabolismo , Caulobacter crescentus/enzimologia , Endorribonucleases/metabolismo , Proteínas de Bactérias/análise , Caulobacter crescentus/metabolismo , Caulobacter crescentus/ultraestrutura , Ciclo Celular , Polaridade Celular , Cromossomos Bacterianos/genética , Cromossomos Bacterianos/ultraestrutura , Endorribonucleases/análise , Regulação Bacteriana da Expressão Gênica , Proteínas Luminescentes/análise , Microscopia de Fluorescência/métodos , Complexos Multienzimáticos/metabolismo , Polirribonucleotídeo Nucleotidiltransferase/metabolismo , RNA Helicases/metabolismo , RNA Bacteriano/biossíntese , RNA Bacteriano/genética , RNA Ribossômico/biossíntese , RNA Ribossômico/genética , Ribossomos/metabolismo , Imagem Individual de Molécula/métodos , Frações Subcelulares/enzimologia , Moldes Genéticos , Transcrição GênicaRESUMO
Piwi-interacting RNAs (piRNAs) are a class of small non-coding RNAs that associate with PIWI subfamily proteins, which play an important role in transposon silencing in animal germ cell. The piRNAs biogenesis is divided into two major pathways: primary and secondary, and both pathways are independent of double-stranded RNA-processing enzyme Dicer, which processes the single-stranded RNA transcripts in microRNA (miRNA) and siRNA (small interfering RNA) pathway. Primary piRNAs are processed from long non-coding RNA precursors transcribed from piRNA clusters. Zucchini (Zuc), a mitochondrial phospholipase D (PLD) superfamily protein is conserved among the animals and involved in piRNA biogenesis. Recent studies showed that the Zucchini is an endoribonuclease essential for primary piRNA maturation and production of phased piRNA in secondary piRNA biogenesis of drosophila germ cell. Based on these reports, here we identified and studied the silkworm Zucchini (BmZuc) at subcellular level in ovary-derived BmN4 cell. The silkworm Zuc specifically expressed in germ-related tissues and localized on mitochondria and partially co-localized with perinuclear nuage-piRNA pathway components and nuage marker protein BmVasa. Molecular dissection analyses revealed that the conserved mitochondrial localization sequence, RGV motif, PLDc 2 domain and HKD motif are important for the BmZuc mitochondrial localization. Moreover, the knockdown analyses showed that the piRNA pathway components are independent on BmZuc for their nuage localization, whereas BmZuc depend on piRNA pathway components for the proper localization. Our data provides vital information on mitochondrial BmZuc and its relationship to "nuage" in ovary-derived BmN4 cell.
Assuntos
Bombyx/metabolismo , Endorribonucleases/metabolismo , Proteínas de Insetos/metabolismo , Mitocôndrias/metabolismo , RNA Interferente Pequeno/metabolismo , Sequência de Aminoácidos , Animais , Bombyx/citologia , Linhagem Celular , Endorribonucleases/análise , Feminino , Proteínas de Insetos/análise , Ovário/citologia , Ovário/metabolismo , RNA Interferente Pequeno/análise , Alinhamento de Sequência , Transdução de SinaisRESUMO
Accumulation of unfolded proteins within the endoplasmic reticulum (ER) triggers a highly conserved stress response mechanism termed the unfolded protein response (UPR). The UPR is a complex series of signaling pathways controlled by ER localized transmembrane receptors, PERK, ATF6 and IRE1α. Following activation IRE1α splices XBP-1 mRNA facilitating the formation of a potent transcription factor, spliced XBP-1. The BCL-2 family members, BAX and BAK, in addition to the mitochondrion also localize to the ER and have been demonstrated to directly interact with IRE1α promoting its activity. In this study we show that in addition to BAX and BAK, the anti-apoptotic BCL-2 protein can regulate IRE1α activity. Enhanced splicing of XBP-1 was observed in BCL-2 overexpressing cells implicating BCL-2 in the complex regulation of IRE1α activity.
Assuntos
Proteínas de Ligação a DNA/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Splicing de RNA , Fatores de Transcrição/genética , Resposta a Proteínas não Dobradas , Animais , Linhagem Celular , Proteínas de Ligação a DNA/análise , Proteínas de Ligação a DNA/metabolismo , Endorribonucleases/análise , Endorribonucleases/metabolismo , Camundongos , Complexos Multienzimáticos/análise , Complexos Multienzimáticos/metabolismo , Proteínas Serina-Treonina Quinases/análise , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Proto-Oncogênicas c-bcl-2/análise , Ratos , Fatores de Transcrição de Fator Regulador X , Transdução de Sinais , Fatores de Transcrição/análise , Fatores de Transcrição/metabolismo , Proteína 1 de Ligação a X-BoxRESUMO
BACKGROUND: The unfolded protein response (UPR) is one of the pathways triggered to ensure quality control of the proteins assembled in the endoplasmic reticulum (ER) when cell homeostasis is compromised. This mechanism is primarily composed of three transmembrane proteins serving as stress sensors: PKR-like ER kinase (PERK), activating transcription factor 6 (ATF6), and inositol-requiring enzyme 1 (IRE1). These three proteins' synergic action elicits translation and transcriptional downstream pathways, leading to less protein production and activating genes that encode important proteins in folding processes, including chaperones. Previous reports showed that viruses have evolved mechanisms to curtail or customize this UPR signaling for their own benefit. However, HIV infection's effect on the UPR has scarcely been investigated. METHODS: This work investigated UPR modulation by HIV infection by assessing UPR-related protein expression under in vitro and in vivo conditions via Western blotting. Antiretroviral (ARV) drugs' influence on this stress response was also considered. RESULTS: In in vitro and in vivo analyses, our results confirm that HIV infection activates stress-response components and that ARV therapy contributes to changes in the UPR's activation profile. CONCLUSIONS: This is the first report showing UPR-related protein expression in HIV target cells derived directly from HIV-infected patients receiving different ARV therapies. Thus, two mechanisms may occur simultaneously: interference by HIV itself and the ARV drugs' pharmacological effects as UPR activators. New evidence of how HIV modulates the UPR to enhance its own replication and secure infection success is also presented.
Assuntos
Fator 6 Ativador da Transcrição/análise , Antirretrovirais/uso terapêutico , Terapia Antirretroviral de Alta Atividade/métodos , Endorribonucleases/análise , Infecções por HIV/tratamento farmacológico , Proteínas Serina-Treonina Quinases/análise , Resposta a Proteínas não Dobradas , eIF-2 Quinase/análise , Adulto , Western Blotting , Feminino , Infecções por HIV/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Adulto JovemRESUMO
Initial steps in the synthesis of functional tRNAs require 5'- and 3'-processing of precursor tRNAs (pre-tRNAs), which in yeast mitochondria are achieved by two endonucleases, RNase P and RNase Z. In this study, using a combination of detergent-free Blue Native Gel Electrophoresis, proteomics and in vitro testing of pre-tRNA maturation, we reveal the physical association of these plus other mitochondrial activities in a large, stable complex of 136 proteins. It contains a total of seven proteins involved in RNA processing including RNase P and RNase Z, five out of six subunits of the mitochondrial RNA degradosome, components of the fatty acid synthesis pathway, translation, metabolism and protein folding. At the RNA level, there are the small and large rRNA subunits and RNase P RNA. Surprisingly, this complex is absent in an oar1Δ deletion mutant of the type II fatty acid synthesis pathway, supporting a recently published functional link between pre-tRNA processing and the FAS II pathway--apparently by integration into a large complex as we demonstrate here. Finally, the question of mt-RNase P localization within mitochondria was investigated, by GFP-tracing of a known protein subunit (Rpm2p). We find that about equal fractions of RNase P are soluble versus membrane-attached.
Assuntos
Endorribonucleases/análise , Proteínas Mitocondriais/análise , RNA de Transferência/metabolismo , Ribonuclease P/análise , Saccharomyces cerevisiae/enzimologia , Ácido Graxo Sintases/genética , Mitocôndrias/enzimologia , Mitocôndrias/genética , Membranas Mitocondriais/enzimologia , Proteínas Mitocondriais/isolamento & purificação , Subunidades Proteicas/análise , Processamento Pós-Transcricional do RNA , Ribonuclease P/isolamento & purificação , Ribonuclease P/metabolismo , Saccharomyces cerevisiae/genética , Proteínas de Saccharomyces cerevisiae/análise , Deleção de SequênciaRESUMO
The mazEFSa toxin-antitoxin (TA) system is ubiquitous in clinical isolates of Staphylococcus aureus, yet its physiological role is unclear. MazFSa is a sequence-specific endoribonuclease that inhibits the growth of S. aureus and Escherichia coli on ectopic overexpression. MazFSa preferentially cleaves RNA at UACAU sites, which are overrepresented in genes encoding pathogenicity factors. The exploitation of the inherent toxicity of MazFSa by artificial toxin activation has been proposed as an antibacterial strategy; however, enzymatic activity of endogenous MazFSa has never been detected, and tools for such analyses are lacking. Here we detail methods for detection of the ribonuclease activity of MazFSa, including a continuous fluorometric assay and a gel-based cleavage assay. Importantly, these methods allowed for the first detection of endogenous MazFSa enzymatic activity in S. aureus lysate. These robust and sensitive assays provide a toolkit for the identification, analysis, and validation of stressors that induce MazF enzymatic activity and should assist in the discovery of artificial activators of the mazEFSa TA system.
Assuntos
Proteínas de Ligação a DNA/análise , Endorribonucleases/análise , Ensaios Enzimáticos , Proteínas de Escherichia coli/análise , Escherichia coli/enzimologia , Fluorometria/métodos , Regulação Bacteriana da Expressão Gênica , Staphylococcus aureus/enzimologia , Antitoxinas/genética , Antitoxinas/metabolismo , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , Proteínas de Ligação a DNA/genética , Proteínas de Ligação a DNA/metabolismo , Eletroforese em Gel de Poliacrilamida , Endorribonucleases/genética , Endorribonucleases/metabolismo , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Proteínas de Escherichia coli/metabolismo , Corantes Fluorescentes , Radioisótopos de Fósforo , Isoformas de Proteínas/análise , Isoformas de Proteínas/genética , Isoformas de Proteínas/metabolismo , Proteínas Recombinantes de Fusão/análise , Proteínas Recombinantes de Fusão/genética , Proteínas Recombinantes de Fusão/metabolismo , Sensibilidade e Especificidade , Staphylococcus aureus/genética , Especificidade por Substrato , Fatores de Virulência/genética , Fatores de Virulência/metabolismoRESUMO
Rhubarb (official Da-huang) is an important medicinal herb in Asia. Many adulterants of official Da-huang have been discovered in Chinese markets in recent years, which has resulted in adverse effects in medicinal treatment. Here, novel molecular markers based on a short maturase K (matK) gene were developed for authenticating official Da-huang. This study showed that all the species from official Da-huang were clustered together in one clade in the polygenetic trees based on short matK. Two highly conserved single nucleotide polymorphisms of short matK were mined in the species from official Da-huang. Based on these polymophisms, four improved specific primers of official Da-huang were successfully developed that generated reproducible specific bands. These results suggest that the short matK sequence can be considered as a favorable candidate for distinguishing official Da-huang from its adulterants. The established multiplex allele-specific PCR was determined to be simple and accurate and may serve as a preferable tool for authentication of official Da-huang. In addition, we suggest that short-sized specific bands be developed to authenticate materials used in traditional Chinese medicine.
Assuntos
Alelos , Endorribonucleases/genética , Reação em Cadeia da Polimerase Multiplex/métodos , Nucleotidiltransferases/genética , Proteínas de Plantas/genética , Rheum/genética , Medicamentos de Ervas Chinesas/química , Endorribonucleases/análise , Genes de Plantas , Marcadores Genéticos , Nucleotidiltransferases/análise , Filogenia , Proteínas de Plantas/análise , Polimorfismo de Nucleotídeo Único , Rheum/química , Rheum/classificação , Análise de Sequência de DNARESUMO
The unfolded protein response (UPR) is a stress response activated upon disturbed homeostasis in the endoplasmic reticulum (ER). Previously, we reported that the activation of the UPR closely correlates with the presence of phosphorylated tau (p-tau) in Alzheimer's disease (AD). As well as increased presence of intracellular p-tau, AD brains are characterized by extracellular deposits of ß amyloid (Aß). Recent in vitro studies have shown that Aß can induce ER stress and activation of the UPR. The aim of the present study is to investigate UPR activation in sporadic tauopathies like progressive supranuclear palsy (PSP) and Pick's disease (PiD), and familial cases with frontotemporal dementia and parkinsonism linked to chromosome 17 (FTDP-17) which carry mutations in the gene encoding for tau (MAPT). The presence of phosphorylated pancreatic ER kinase (pPERK) and phosphorylated inositol requiring enzyme 1α (pIRE1), which are indicative of an activated UPR, was assessed by immunohistochemistry in cases neuropathologically defined as frontotemporal lobar degeneration with tau pathology (FTLD-tau). Increased presence of UPR activation markers pPERK and pIRE1 was observed in neurons and glia in FTLD-tau cases, in contrast to FTLD subtypes negative for tau pathology or in non-neurological controls. pPERK and pIRE1 were also prominently present in relatively young carriers of MAPT mutation. A strong association between the presence of UPR activation markers and p-tau was observed in the hippocampus of FTLD-tau cases. Double immunohistochemical staining on FTLD-tau cases revealed that UPR activation is predominantly observed in neurons that show diffuse staining of p-tau. These data demonstrate that UPR activation is intimately connected with the accumulation and aggregation of p-tau, and occurs independently from Aß deposits. Our findings provide new pathological insight into the close association between p-tau and UPR activation in tauopathies.
Assuntos
Hipocampo/química , Tauopatias/metabolismo , Resposta a Proteínas não Dobradas , Proteínas tau/análise , Adulto , Idoso , Idoso de 80 Anos ou mais , Autopsia , Biomarcadores/análise , Estudos de Casos e Controles , Endorribonucleases/análise , Feminino , Hipocampo/patologia , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Mutação , Fosforilação , Proteínas Serina-Treonina Quinases/análise , Tauopatias/genética , Regulação para Cima , eIF-2 Quinase/análise , Proteínas tau/genéticaRESUMO
Polycomb group (PcG) proteins are key regulators of stem-cell and cancer biology. They mainly act as repressors of differentiation and tumor-suppressor genes. One key silencing step involves the trimethylation of histone H3 on Lys27 (H3K27) by EZH2, a core component of the Polycomb Repressive Complex 2 (PRC2). The mechanism underlying the initial recruitment of mammalian PRC2 complexes is not well understood. Here, we show that NIPP1, a regulator of protein Ser/Thr phosphatase-1 (PP1), forms a complex with PP1 and PRC2 components on chromatin. The knockdown of NIPP1 or PP1 reduced the association of EZH2 with a subset of its target genes, whereas the overexpression of NIPP1 resulted in a retargeting of EZH2 from fully repressed to partially active PcG targets. However, the expression of a PP1-binding mutant of NIPP1 (NIPP1m) did not cause a redistribution of EZH2. Moreover, mapping of the chromatin binding sites with the DamID technique revealed that NIPP1 was associated with multiple PcG target genes, including the Homeobox A cluster, whereas NIPP1m showed a deficient binding at these loci. We propose that NIPP1 associates with a subset of PcG targets in a PP1-dependent manner and thereby contributes to the recruitment of the PRC2 complex.
Assuntos
Cromatina/metabolismo , Proteínas de Ligação a DNA/análise , Endorribonucleases/metabolismo , Histona-Lisina N-Metiltransferase/análise , Fosfoproteínas Fosfatases/metabolismo , Proteínas de Ligação a RNA/metabolismo , Proteínas Repressoras/metabolismo , Fatores de Transcrição/análise , Sítios de Ligação , Linhagem Celular , Cromatina/química , Cromatina/enzimologia , Endorribonucleases/análise , Endorribonucleases/antagonistas & inibidores , Proteína Potenciadora do Homólogo 2 de Zeste , Histona Metiltransferases , Humanos , Fosfoproteínas Fosfatases/análise , Fosfoproteínas Fosfatases/antagonistas & inibidores , Complexo Repressor Polycomb 2 , Proteínas do Grupo Polycomb , Regiões Promotoras Genéticas , Proteína Fosfatase 1/antagonistas & inibidores , Proteína Fosfatase 1/metabolismo , Proteína Fosfatase 1/fisiologia , Interferência de RNA , Proteínas de Ligação a RNA/análise , Proteínas de Ligação a RNA/antagonistas & inibidoresRESUMO
The human scavenger decapping enzyme, DcpS, functions to hydrolyze the resulting cap structure following cytoplasmic mRNA decay yet is, surprisingly, a nuclear protein by immunofluorescence. Here, we show that DcpS is a nucleocytoplasmic shuttling protein that contains separable nuclear import and Crm-1-dependent export signals. We postulated that the presence of DcpS in both cellular compartments and its ability to hydrolyze cap structure may impact other cellular events dependent on cap-binding proteins. An shRNA-engineered cell line with markedly diminished DcpS levels led to a corresponding reduction in cap-proximal intron splicing of a reporter minigene and endogenous genes. The impaired cap catabolism and resultant imbalanced cap concentrations were postulated to sequester the cap-binding complex (CBC) from its normal splicing function. In support of this explanation, DcpS efficiently displaced the nuclear cap-binding protein Cbp20 from cap structure, and complementation with Cbp20 reversed the reduced splicing, indicating that modulation of splicing by DcpS is mediated through Cbp20. Our studies demonstrate that the significance of DcpS extends beyond its well-characterized role in mRNA decay and involves a broader range of functions in RNA processing including nuclear pre-mRNA splicing.
Assuntos
Endorribonucleases/metabolismo , Precursores de RNA/metabolismo , Splicing de RNA , Transporte Ativo do Núcleo Celular , Sequência de Aminoácidos , Animais , Linhagem Celular , Núcleo Celular/química , Núcleo Celular/metabolismo , Citoplasma/química , Citoplasma/metabolismo , Endorribonucleases/análise , Endorribonucleases/genética , Células HeLa , Humanos , Íntrons , Camundongos , Dados de Sequência Molecular , Células NIH 3T3 , Sinais de Exportação Nuclear/genética , Sinais de Localização Nuclear/análise , Sinais de Localização Nuclear/genética , Sinais de Localização Nuclear/metabolismo , Splicing de RNA/genética , RatosRESUMO
In mammalian cells, the enzymatic pathways involved in cytoplasmic mRNA decay are incompletely defined. In this study, we have used two approaches to disrupt activities of deadenylating and/or decapping enzymes to monitor effects on mRNA decay kinetics and trap decay intermediates. Our results show that deadenylation is the key first step that triggers decay of both wild-type stable and nonsense codon-containing unstable beta-globin mRNAs in mouse NIH3T3 fibroblasts. PAN2 and CCR4 are the major poly(A) nucleases active in cytoplasmic deadenylation that have biphasic kinetics, with PAN2 initiating deadenylation followed by CCR4-mediated poly(A) shortening. DCP2-mediated decapping takes place after deadenylation and may serve as a backup mechanism for triggering mRNA decay when initial deadenylation by PAN2 is compromised. Our findings reveal a functional link between deadenylation and decapping and help to define in vivo pathways for mammalian cytoplasmic mRNA decay.
Assuntos
Endorribonucleases/metabolismo , Exorribonucleases/metabolismo , Estabilidade de RNA/genética , RNA Mensageiro/metabolismo , Animais , Núcleo Celular/enzimologia , Citoplasma/metabolismo , Endorribonucleases/análise , Endorribonucleases/genética , Exorribonucleases/análise , Exorribonucleases/genética , Fibroblastos/enzimologia , Globinas/genética , Humanos , Camundongos , Células NIH 3T3 , Interferência de RNARESUMO
Sera from patients with autoimmune diseases often contain antibodies that bind ribonucleoproteins (RNPs). Sera from 30 such patients were found to immunoprecipitate ribonuclease P (RNase P), an RNP enzyme required to process the 5' termini of transfer RNA transcripts in nuclei and mitochondria of eukaryotic cells. All 30 sera also immunoprecipitated the nucleolar Th RNP, indicating that the two RNPs are structurally related. Nucleotide sequence analysis of the Th RNP revealed it was identical to the RNA component of the mitochondrial RNA processing enzyme known as RNase MRP. Antibodies that immunoprecipitated the Th RNP selectively depleted murine and human cell extracts of RNase MRP activity, indicating that the Th and RNase MRP RNPs are identical. Since RNase P and RNase MRP are not associated with each other during biochemical purification, we suggest that these two RNA processing enzymes share a common autoantigenic polypeptide.
Assuntos
Autoantígenos , Endorribonucleases , Processamento Pós-Transcricional do RNA , Ribonucleoproteínas , Sequência de Bases , Núcleo Celular/enzimologia , Endorribonucleases/análise , Endorribonucleases/imunologia , Humanos , Mitocôndrias/enzimologia , Dados de Sequência Molecular , RNA/análise , Ribonuclease PRESUMO
Ribonuclease P is a ribonucleoprotein that cleaves precursors to transfer RNA (tRNA) molecules to yield the correct 5' terminal sequences of the mature tRNA's. The RNA moiety M1 RNA of ribonuclease P from Escherichia coli and the unprocessed transcript prepared in vitro of the gene for M1 RNA can both perform the cleavage reactions of the canonical enzyme in the absence of the protein moiety. When the transcript of the M1 RNA gene is combined with the protein moiety not only is a tRNA precursor cleaved but also the precursor to 4.5S RNA from Escherichia coli.
Assuntos
Endorribonucleases/metabolismo , Proteínas de Escherichia coli , Escherichia coli/enzimologia , Precursores de Ácido Nucleico/metabolismo , RNA Bacteriano/metabolismo , Aminoacil-RNA de Transferência/metabolismo , RNA/metabolismo , Sequência de Bases , Catálise , Endorribonucleases/análise , Precursores de RNA , RNA Bacteriano/genética , Ribonuclease P , Transcrição GênicaRESUMO
The gene for the RNA subunit (M1 RNA) of ribonuclease P from Salmonella typhimurium directs the synthesis of an RNA that can cleave transfer RNA precursor molecules. The mature M1 RNA coded for by Salmonella typhimurium is 375 nucleotides long and has six nucleotide changes in comparison to M1 RNA from Escherichia coli. The regions for promotion and termination of transcription are closely conserved, but adjacent regions of nucleotide sequences show considerable drift.
Assuntos
Endorribonucleases/análise , Proteínas de Escherichia coli , RNA Bacteriano/genética , Salmonella typhimurium/genética , Sequência de Bases , Clonagem Molecular , Escherichia coli/enzimologia , Escherichia coli/genética , Genes Bacterianos , Conformação de Ácido Nucleico , Precursores de Ácido Nucleico/genética , Regiões Promotoras Genéticas , RNA/genética , Precursores de RNA , RNA Bacteriano/metabolismo , Sequências Repetitivas de Ácido Nucleico , Ribonuclease P , Salmonella typhimurium/enzimologia , Regiões Terminadoras Genéticas , Transcrição GênicaRESUMO
The efficiency of the Serratia marcescens nuclease encoded by the NucA gene, with or without a nuclear localization signal (NLS), and the commonly used diphtheria toxin A (DTA) were compared for their ability to ablate cells in culture. Constructs containing the test genes driven by the beta-actin promoter coupled with enhancer elements from the cytomegalovirus promoter and rabbit beta-globin gene (pCAG) and the blasticidin resistance gene driven by the phosphoglycerate kinase (PGK) promoter were generated and electroporated into porcine fetal fibroblasts. Three independent replicates were completed. Following blasticidin selection, the number of surviving colonies was counted to assess the efficiency of the toxic gene. Both NucA and DTA proved to be effective in killing porcine fibroblasts compared to controls. However, the efficiency of cell ablation was significantly higher with DTA than with NucA or NucANLS (p < 0.05). Gene expression analysis of surviving colonies indicated that survival is related to low or absent expression of the toxic genes. These results indicate that the NucA gene, while capable of mammalian cell ablation, is less efficient than DTA.
Assuntos
Separação Celular/métodos , Eletroporação/métodos , Endodesoxirribonucleases/análise , Endorribonucleases/análise , Técnicas de Transferência de Genes , Serratia/enzimologia , Animais , Células Cultivadas , Endodesoxirribonucleases/genética , Endodesoxirribonucleases/metabolismo , Endorribonucleases/genética , Endorribonucleases/metabolismo , Fibroblastos/metabolismo , Expressão Gênica , Coelhos , SuínosRESUMO
We identified human decapping enzyme 2 (hDCP2) as a binding protein with Ro52, being colocalized in processing bodies (p-bodies). We also showed that the N-terminus and C-terminus of Ro52 bound to hDCP2. Moreover, Ro52 enhanced decapping activity of hDCP2 in a dose-dependent manner. Our data support the novel notion of the association between Ro52 with hDCP2 protein in cytoplasmic p-bodies, playing a role in mRNA metabolism in response to cellular stimulation.
Assuntos
Autoantígenos/metabolismo , Endorribonucleases/metabolismo , Capuzes de RNA/metabolismo , Estabilidade de RNA , Ribonucleoproteínas/metabolismo , Autoantígenos/análise , Catálise , Citoplasma/enzimologia , Endorribonucleases/análise , Endorribonucleases/genética , Humanos , Mapeamento de Interação de Proteínas , Estrutura Terciária de Proteína , Ribonucleoproteínas/análise , Ribonucleoproteínas/genéticaRESUMO
BACKGROUND: Ribonucleases of T2 family are ubiquitous cellular components which have played several biological functions in molecular and pharmaceutical fields. OBJECTIVE: Therefore, a soluble and highly active RNase belonging to T2 family was screened from Bacillus megaterium NRRL 3712, and different cultivation strategies were applied to enhance the production of enzyme. METHOD: A high-level of an extracellular RNase and cell density was produced using optimal cultivation conditions. A monomeric enzyme with a molecular mass of 45 kDa, was purified to homogeneity using acetone precipitation and ion-exchange chromatography. RESULTS: Purified enzyme was optimally activity at 45°C and pH 7.0, and it displayed a half-life of 26 min at 64°C. It was quite stable up to 60 min at 40-50°C temperature and over a broad range of pH 4.5-8.0. It showed great substrate specificity with yeast RNA, poly (A), poly (G), poly (C), and poly (U). Kinetic parameters such as Km, Vmax, kcat and kcat Km -1 values against yeast RNA as substrate, were 71.67 µg mL-1, 7866.4 µmol mg-1min-1, 17669.4 sec-1, and 246.53, respectively. CONCLUSION: The article provides a valuable novel RNase which exhibited great resistance against various organic solvents, detergents and metal ions, whereas its activity was stimulated up to 142% by adding 5 mM EDTA. Hence, dictates its applicability as therapeutic agent and in various other biotechnological fields.
Assuntos
Bacillus megaterium/enzimologia , Endorribonucleases/isolamento & purificação , Cátions , Cromatografia por Troca Iônica , Detergentes/química , Endorribonucleases/análise , Estabilidade Enzimática , Concentração de Íons de Hidrogênio , Cinética , Metais/química , Peso Molecular , Solventes/química , Especificidade por Substrato , TemperaturaRESUMO
In response to a variety of insults the unfolded protein response (UPR) is a major cell program quickly engaged to promote either cell survival or if stress levels cannot be relieved, apoptosis. UPR relies on three major pathways, named from the endoplasmic reticulum (ER) resident proteins IRE1α, PERK, and ATF6 that mediate response. Current tools to measure the activation of these ER stress response pathways in mammalian cells are cumbersome and not compatible with high-throughput imaging. In this study, we present IRE1α and PERK sensors with improved sensitivity, based on the canonical events of xbp1 splicing and ATF4 translation at ORF3. These sensors can be integrated into host cell genomes through lentiviral transduction, opening the way for use in a wide array of immortalized or primary mammalian cells. We demonstrate that high-throughput single-cell analysis offers unprecedented kinetic details compared with endpoint measurement of IRE1α and PERK activity. Finally, we point out the limitations of dye-based nuclear segmentation for live cell imaging applications, as we show that these dyes induce UPR and can strongly affect both the kinetic and dynamic responses of IRE1α and PERK pathways.
Assuntos
Corantes/química , Endorribonucleases/análise , Imagem Óptica , Proteínas Serina-Treonina Quinases/análise , eIF-2 Quinase/análise , Células Cultivadas , Estresse do Retículo Endoplasmático , Endorribonucleases/metabolismo , Células HCT116 , Células HEK293 , Células HeLa , Ensaios de Triagem em Larga Escala , Humanos , Proteínas Serina-Treonina Quinases/metabolismo , Análise de Célula Única , eIF-2 Quinase/metabolismoRESUMO
BACKGROUND: Patients with chronic fatigue syndrome (CFS) present a disordered sleep pattern and frequently undergo polysomnography to exclude a primary sleep disorder. Such studies have shown reduced sleep efficiency, a reduction of deep sleep, prolonged sleep initiation, and alpha-wave intrusion during deep sleep. Deregulation of the 2-5A synthetase/RNase L antiviral pathway and a potential acquired channelopathy are also found in a subset of CFS patients and could lead to sleep disturbances. This article compiles a large sleep study database on CFS patients and correlates these data with a limited number of immune parameters as it has been thought that RNase L could be associated with these sleep disturbances. METHODS: Forty-eight patients who fulfilled 1994 Centers for Disease Control and Prevention criteria for CFS underwent extensive medical evaluation, routine laboratory testing, and a structured psychiatric interview. Subjects then completed a complaint checklist and a two-night polysomnographic investigation. RNase L analysis was performed by gel electrophoresis using a radiolabeled 2',5'-oligoadenylate trimer. Basic descriptive statistical parameters were calculated. RESULTS: Patients experienced a prolonged sleep latency, showed a low sleep efficiency index, and had a low percentage of slow wave sleep. The present alpha-delta intrusion correlated with anxiety; no correlations appeared, however, between alpha-delta sleep and immunologic parameters, including RNase L. CONCLUSIONS: The main findings are 1) validation of sleep latency problems and other sleep disturbances as already suggested by several authors; 2) alpha-delta intrusion seems associated with anxiety; and 3) elevated RNase L did not correlate with alpha-delta sleep.
Assuntos
Ansiedade/diagnóstico , Síndrome de Fadiga Crônica/complicações , Fases do Sono , Transtornos do Sono-Vigília/diagnóstico , Adulto , Idoso , Ritmo alfa , Antígenos CD/análise , Ansiedade/complicações , Ritmo Delta , Endorribonucleases/análise , Síndrome de Fadiga Crônica/imunologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Polissonografia , Transtornos do Sono-Vigília/complicaçõesRESUMO
BACKGROUND: The role of epigenetics in gene expression regulation and development significantly enhances our understanding of carcinogenesis. All the tumor related genes may be the target of epigenetical or genetic regulation. We selected some epigenetically regulated genes for cDNA array analysis and observed variability in the expression of the DICER1 gene in distinct stages of gastric cancer. The aim of this study was to assess the correlation between the expression of DICER1, an epigenetically regulated gene, and gastric cancer. METHODS: To detect the expression of 506 tumor-associated genes, including DICER1, in the matched cancerous mucosa, pre-malignant lesion (adjacent mucosa), non-cancerous gastric mucosa and distant lymphocyte metastatic lesion in 3 cases of gastric cancers using cDNA array. DICER1 mRNA expression and DICER1 protein expression were further analyzed by Real-time PCR and Western blot in 32 cases of progressive gastric cancer. DICER1 protein expression was also detected in 33 early and 30 progressive gastric cancers by the immunohistochemistry (IHC) method. RESULTS: In 3 cases of gastric cancer cDNA array showed dramatically decreased expression of DICER1 in pre-malignant lesion, cancerous mucosa and distant lymphocyte metastatic lesions compared with matched noncancerous gastric mucosa, pre-malignant lesion and cancerous mucosa. Real-time PCR results showed that the expression level of DICER1 mRNA in gastric cancer was significantly down-regulated compared to normal gastric tissue (P < 0.05). The IHC assay also showed that the expression of DICER1 was significantly decreased in progressive gastric cancer. Among the 63 cases of gastric cancers, 13/33 early (39.4%) and 19/30 (63.3%) progressive cancers showed negative expression of DICER1 (50.8%). The difference in expression of DICER1 between early and progressive gastric cancers was significant (P < 0.01). The result of Western blotting showed that DICER1 protein was down-regulated significantly in advanced gastric cancer (P < 0.05). CONCLUSIONS: DICER1 expression is decreased during the progression of gastric cancer, especially in progressive gastric cancers, which indicating DICER1 may play an important role in the development of cancer and the epigenetical regulation involved.