RESUMO
In this study, the development of our purpose-made capacitively coupled contactless conductivity detection (C4 D) for CE is reported. These systems have been employed as a simple, versatile, and cost-effective analytical tool. CE-C4 D devices, whose principle is based on the control of the ion movements under an electrical field, can be constructed even with a modest financial budget and limited infrastructure. A featured application was developed for quality control of antimicrobial drugs using CE-C4 D, with most recent work on determination of aminoglycoside and glycopeptide antibiotics being communicated. For aminoglycosides, the development of CE-C4 D methods was adapted to two categories. The first one includes drugs (liquid or powder form) for intravenous injection, containing either amikacin, streptomycin, kanamycin A, or kanamycin B. The second one covers drugs for eye drops (liquid or ointment form), containing either neomycin, tobramycin, or polymyxin. The CE-C4 D method development was also made for determination of some popular glycopeptide antibiotics in Vietnam, including vancomycin and teicoplanin. The best detection limit achieved using the developed CE-C4 D methods was 0.5 mg/L. Good agreement between results from CE-C4 D and the confirmation method (HPLC- Photometric Diode Array ) was achieved, with their result deviations less than 8% and 13% for aminoglycoside and glycopeptide antibiotics, respectively.
Assuntos
Antibacterianos , Eletroforese Capilar/métodos , Aminoglicosídeos/análise , Aminoglicosídeos/química , Aminoglicosídeos/normas , Antibacterianos/análise , Antibacterianos/química , Antibacterianos/normas , Condutividade Elétrica , Eletroforese Capilar/economia , Eletroforese Capilar/instrumentação , Desenho de Equipamento , Glicopeptídeos/análise , Glicopeptídeos/química , Glicopeptídeos/normas , Limite de Detecção , Modelos Lineares , Controle de Qualidade , Reprodutibilidade dos Testes , VietnãAssuntos
Antitrombina III/análise , Cromatografia Líquida de Alta Pressão , Espectrometria de Massas em Tandem , Sequência de Aminoácidos , Antitrombina III/metabolismo , Antitrombina III/normas , Calibragem , Cromatografia Líquida de Alta Pressão/normas , Quimotripsina/metabolismo , Glicopeptídeos/análise , Glicopeptídeos/isolamento & purificação , Glicopeptídeos/normas , Peptídeos/análise , Peptídeos/isolamento & purificação , Peptídeos/normas , Padrões de Referência , Extração em Fase Sólida , Espectrometria de Massas em Tandem/normas , Tripsina/metabolismoRESUMO
Absolute quantitation of IgG-1 Fc-glycosylation, which is crucial for the clinical practice of glyco-biomarkers and quality control of biopharmaceuticals, has been hindered by the lack of glycopeptide standards. In this study, eleven high abundant IgG-1 Fc-glycopeptides with definite peptide sequences and glycoforms were purified from commercial IgG protein by using two-dimensional hydrophilic interaction liquid chromatographic system. Based on the acquired glycopeptide standards, an absolute quantitation strategy was developed to determine the concentrations of 11 target IgG-1 glycopeptides from pooled human sera. A wide range of Fc-glycopeptide concentrations from 0.60 to 17.61 nmol mL-1 was achieved with excellent accuracy and reproducibility from pooled human sera IgG-1. Compared to conventional relative quantitation, this strategy provides more accurate distribution profiles of 11 high abundant Fc-glycopeptides and degree of glycosylation from pooled human sera IgG-1.
Assuntos
Glicopeptídeos/sangue , Fragmentos Fc das Imunoglobulinas/sangue , Imunoglobulina G/sangue , Sequência de Carboidratos , Cromatografia Líquida/métodos , Cromatografia Líquida/normas , Glicopeptídeos/química , Glicopeptídeos/isolamento & purificação , Glicopeptídeos/normas , Humanos , Interações Hidrofóbicas e Hidrofílicas , Fragmentos Fc das Imunoglobulinas/química , Fragmentos Fc das Imunoglobulinas/isolamento & purificação , Imunoglobulina G/química , Imunoglobulina G/isolamento & purificação , Limite de Detecção , Reprodutibilidade dos TestesAssuntos
Adrenomedulina/sangue , Fator Natriurético Atrial/sangue , Glicopeptídeos/sangue , Insuficiência Cardíaca/sangue , Hipertensão/sangue , Isquemia Miocárdica/sangue , Peptídeo Natriurético Encefálico/sangue , Fragmentos de Peptídeos/sangue , Precursores de Proteínas/sangue , Adrenomedulina/normas , Idoso , Fator Natriurético Atrial/normas , Biomarcadores/sangue , Feminino , Glicopeptídeos/normas , Insuficiência Cardíaca/diagnóstico , Humanos , Hipertensão/diagnóstico , Masculino , Pessoa de Meia-Idade , Isquemia Miocárdica/diagnóstico , Peptídeo Natriurético Encefálico/normas , Fragmentos de Peptídeos/normas , Precursores de Proteínas/normas , Volume Sistólico/fisiologiaRESUMO
An international standard for teicoplanin has been established on the basis of a collaborative study. There were five participating laboratories in five countries. The activity of the contents of each ampoule of the international standard for teicoplanin is defined as 51,550 IU of teicoplanin.