RESUMO
A study was undertaken to observe morphological changes and viral morphogenesis during the growth of the mouse preputial gland tumor ESR 586. The acinar cells of the normal preputial gland have an extensive agranular endoplasmic reticulum and Golgi apparatus and large lipid droplets. No viral particles are present. During tumor growth, and numerous lipid droplets never attain the size of those found in the normal gland. There is a decrease in the Golgi apparatus and agranular endoplasmic reticulum and an increase in the rough endoplasmic reticulum which could reflect a change in composition of tumor secretory product from the normal gland. Indeed, there is a decrease in triglycerides as the tumor ages and an increase in the sterol esters and waxes. In addition, intracisternal A-particles are observed budding from thickened endoplasmic reticulum membranes. Thickening of these membranes occur early in A-particle formation. One side of the membrane is first thickened while the opposing membrane appears is first thickened while the opposing membrane appears morphologically unaffected. The thickening of the affected membrane is initially confined to the outer (cytoplasmic) face of the membrane. In the older tumor, both opposing membranes of the reticulum are thickened and can assume an elongated whorled pattern.
Assuntos
Adenocarcinoma/ultraestrutura , Neoplasias Penianas/ultraestrutura , Vírus de RNA/crescimento & desenvolvimento , Neoplasias das Glândulas Sebáceas/ultraestrutura , Adenocarcinoma/análise , Adenocarcinoma/microbiologia , Animais , Retículo Endoplasmático/microbiologia , Retículo Endoplasmático/ultraestrutura , Complexo de Golgi/ultraestrutura , Lipídeos/análise , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Mitocôndrias/ultraestrutura , Neoplasias Experimentais/análise , Neoplasias Experimentais/microbiologia , Neoplasias Experimentais/ultraestrutura , Neoplasias Penianas/análise , Neoplasias Penianas/microbiologia , Vírus de RNA/ultraestrutura , Neoplasias das Glândulas Sebáceas/análise , Neoplasias das Glândulas Sebáceas/microbiologiaRESUMO
In this study a transplantable sebaceous adenoma is presented, which maintained its histological structure with similarities to normal sebaceous glands over a long series of transplantations. From the periphery toward the center a basal lamina, an outer layer of undifferenciated stem cells, loosely connected secreting tissue, a tightly packed zone of close cell attachment, and finally a central sebaceous zone can be distinguished. Special reference is made to the belt-like region around the zone of cell lysis and sebum accumulation, which forms a barrier to the surrounding tissue by means of numerous cell adhesions in the form of desmosomes. Relatively few virus particles of the retrovirus group, particularly type A particles, were found in any of the examined tumors.
Assuntos
Adenoma/ultraestrutura , Comunicação Celular , Retroviridae/patogenicidade , Neoplasias das Glândulas Sebáceas/ultraestrutura , Animais , Desmossomos/ultraestrutura , Masculino , Camundongos , Camundongos Endogâmicos , Microscopia Eletrônica , Transplante de Neoplasias , Neoplasias Experimentais/ultraestrutura , Neoplasias das Glândulas Sebáceas/microbiologiaRESUMO
Demodex folliculorum is a saprophytic mite of the human pilosebaceous unit with a prelidiction for facial skin and eyelashes. Infestation occurs frequently without symptoms; however, suppurative or granulomatous inflammation may be seen with high mite density. Skin lesions, such as rosacea, pityriasis, and blepharitis, have been attributed to Demodex; however, its role in sebaceous adenoma (SA) has not been reported in extensively searched medical literature. We report this rare case and review the skin lesions associated with Demodex folliculorum.
Assuntos
Adenoma/microbiologia , Infestações por Ácaros/complicações , Neoplasias das Glândulas Sebáceas/microbiologia , Dermatopatias/microbiologia , Adenoma/patologia , Animais , Humanos , Inseticidas/uso terapêutico , Masculino , Pessoa de Meia-Idade , Infestações por Ácaros/tratamento farmacológico , Infestações por Ácaros/patologia , Ácaros , Permetrina/uso terapêutico , Neoplasias das Glândulas Sebáceas/patologia , Dermatopatias/tratamento farmacológico , Dermatopatias/patologiaRESUMO
Histology and ultrastructure of a sebaceous adenoma of the mouse -- derived from i.p.-injection of 0.1 ml DNA, isolated from cells of a transplantable mouse-leukemia -- were studied. The original tumour and all transplanted tumours display the same tissular differentiation. In all tumours virus particles were found. Structure and behaviour of the intracytoplasmic A-particles resemble those of the mouse mammary tumour virus while the C-particles found extracellular were identical with the leukemia viruses of the mouse. It might be suspected that mammary tumour virus and leukemia viruses are either non specific or that a new virus is present in the sebaceous adenoma.
Assuntos
Adenoma/microbiologia , Corpos de Inclusão Viral , Neoplasias das Glândulas Sebáceas/microbiologia , Adenoma/patologia , Animais , Feminino , Masculino , Vírus do Tumor Mamário do Camundongo , Camundongos , Camundongos Endogâmicos , Microscopia Eletrônica , Transplante de Neoplasias , Neoplasias Experimentais/microbiologia , Retroviridae , Neoplasias das Glândulas Sebáceas/patologia , Transplante HomólogoRESUMO
Papillomaviruses, group-specific papillomavirus antigens, or extrachromosomal papillomavirus DNA were detected in cutaneous, mucocutaneous, and pulmonary tumors affecting a colony of European harvest mice (Micromys minutus). Skin lesions were classified as acanthomatous hyperplasia, epidermal inclusion cysts, squamous papillomas, inverted papillomas, trichoepitheliomas, and sebaceous carcinomas. Cutaneous horns (hyperkeratotic papillomas) were on mucocutaneous junctions of one animal. One mouse, with a cutaneous sebaceous carcinoma, had multiple pulmonary keratinaceous cysts. Papillomavirus antigens, detected by the avidin-biotin technique, were in 20 of 31 lesions tested. In contrast, by Southern blot hybridization all 28 lesions tested contained papillomavirus DNA. Papillomavirus DNA was demonstrated in two of ten benign cutaneous lesions by in situ hybridization.