RESUMO
Purpose: To characterize intermediate aggregate species on the aggregation pathway of γD-crystallin protein in ultraviolet (UV)-C light. Methods: The kinetics of γD-crystallin protein aggregation was studied with reversed-phase high-performance liquid chromatography (RP-HPLC) sedimentation assay, ThT binding assay, and light scattering. We used analytical ultracentrifugation to recognize intermediate aggregate species and characterized them with Fourier transform infrared spectroscopy (FTIR). Quantification of free sulfhydryl groups in an ongoing aggregation reaction was achieved by using Ellman's assay. Results: Negligible lag phase was found in the aggregation kinetic experiments of the γD-crystallin protein. Dimer, tetramer, octamer, and higher oligomer intermediates were formed on the aggregation pathway. The protein changes its conformation to form intermediate aggregate species. FTIR and trypsin digestion indicated structural differences between the protein monomer, intermediate aggregate species, and fibrils. Ellman's assay revealed that disulfide bonds were formed in the protein monomers and aggregates during the aggregation process. Conclusions: This study showed that various intermediate and structurally different aggregate species are formed on the aggregation pathway of γD-crystallin protein in UV-C light.
Assuntos
Agregados Proteicos/efeitos da radiação , Raios Ultravioleta , gama-Cristalinas/química , gama-Cristalinas/efeitos da radiação , Sequência de Aminoácidos , Cromatografia Líquida de Alta Pressão , Humanos , Microscopia de Força Atômica , Modelos Moleculares , Agregação Patológica de Proteínas , Domínios Proteicos , Espectroscopia de Infravermelho com Transformada de Fourier , UltracentrifugaçãoRESUMO
Although cataracts are a well-known age-related disease, the mechanism of their formation is not well understood. It is currently thought that eye lens proteins become abnormally aggregated, initially causing clumping that scatters the light and interferes with focusing on the retina, and ultimately resulting in a cataract. The abnormal aggregation of lens proteins is considered to be triggered by various post-translational modifications, such as oxidation, deamidation, truncation and isomerization, that occur during the aging process. Such modifications, which are also generated by free radical and reactive oxygen species derived from γ-irradiation, decrease crystallin solubility and lens transparency, and ultimately lead to the development of a cataract. In this study, we irradiated young rat lenses with low-dose γ-rays and extracted the water-soluble and insoluble protein fractions. The water-soluble and water-insoluble lens proteins were digested with trypsin, and the resulting peptides were analyzed by LC-MS. Specific oxidation sites of methionine, cysteine and tryptophan in rat water-soluble and -insoluble γE and γF-crystallin were determined by one-shot analysis. The oxidation sites in rat γE and γF-crystallin resemble those previously identified in γC and γD-crystallin from human age-related cataracts. Our study on modifications of crystallins induced by ionizing irradiation may provide useful information relevant to human senile cataract formation.
Assuntos
Raios gama/efeitos adversos , Cristalino/metabolismo , Cristalino/efeitos da radiação , gama-Cristalinas/metabolismo , gama-Cristalinas/efeitos da radiação , Sequência de Aminoácidos , Aminoácidos/química , Animais , Catarata/etiologia , Catarata/metabolismo , Cristalografia por Raios X , Humanos , Cristalino/química , Masculino , Modelos Moleculares , Dados de Sequência Molecular , Oxirredução , Conformação Proteica , Ratos , Ratos Wistar , Solubilidade , Espectrometria de Massas em Tandem , gama-Cristalinas/químicaRESUMO
Ultraviolet radiation (UVR) exposure is a major risk factor for age-related cataract, a protein-aggregation disease of the human lens often involving the major proteins of the lens, the crystallins. γD-Crystallin (HγD-Crys) is abundant in the nucleus of the human lens, and its folding and aggregation have been extensively studied. Previous work showed that HγD-Crys photoaggregates in vitro upon exposure to UVA/UVB light and that its conserved tryptophans are not required for aggregation. Surprisingly, the tryptophan residues play a photoprotective role because of a distinctive energy-transfer mechanism. HγD-Crys also contains 14 tyrosine residues, 12 of which are organized as six pairs. We investigated the role of the tyrosines of HγD-Crys by replacing pairs with alanines and monitoring photoaggregation using light scattering and SDS-PAGE. Mutating both tyrosines in the Y16/Y28 pair to alanine slowed the formation of light-scattering aggregates. Further mutant studies implicated Y16 as important for photoaggregation. Mass spectrometry revealed that C18, in contact with Y16, is heavily oxidized during UVR exposure. Analysis of multiple mutant proteins by mass spectrometry suggested that Y16 and C18 likely participate in the same photochemical process. The data suggest an initial photoaggregation pathway for HγD-Crys in which excited-state Y16 interacts with C18, initiating radical polymerization.
Assuntos
Cisteína/efeitos da radiação , Tirosina/efeitos da radiação , Raios Ultravioleta , gama-Cristalinas/efeitos da radiação , Substituição de Aminoácidos , Catarata/genética , Catarata/metabolismo , Cisteína/química , Cisteína/genética , Humanos , Cristalino/metabolismo , Desnaturação Proteica , Dobramento de Proteína , Estrutura Quaternária de Proteína/efeitos da radiação , Triptofano/química , Tirosina/química , Tirosina/genética , gama-Cristalinas/genéticaRESUMO
γD-Crystallin is an abundant structural protein of the lens that is found in native and modified forms in cataractous aggregates. We establish that UV-B irradiation of γD-Crystallin leads to structurally specific modifications and precipitation via two mechanisms: amorphous aggregates and amyloid fibers. UV-B radiation causes cleavage of the backbone, in large measure near the interdomain interface, where side chain oxidations are also concentrated. 2D IR spectroscopy and expressed protein ligation localize fiber formation exclusively to the C-terminal domain of γD-Crystallin. The native ß-sandwich domains are not retained upon precipitation by either mechanism. The similarities between the amyloid forming pathways when induced by either UV-B radiation or low pH suggest that the propensity for the C-terminal ß-sandwich domain to form amyloid ß-sheets determines the misfolding pathway independent of the mechanism of denaturation.
Assuntos
Amiloide/química , Cristalino/efeitos da radiação , Raios Ultravioleta , gama-Cristalinas/efeitos da radiação , Sequência de Aminoácidos , Peptídeos beta-Amiloides/química , Catarata/etiologia , Humanos , Microscopia Eletrônica de Transmissão , Desnaturação Proteica , Estrutura Quaternária de Proteína , Estrutura Secundária de Proteína , Espectrofotometria Infravermelho , gama-Cristalinas/químicaRESUMO
PURPOSE: Human γD crystallin is a principal protein component of the human eye lens and associated with the development of juvenile and mature-onset cataracts. Exposure to ultraviolet (UV) light is thought to perturb protein structure and eventually lead to aggregation. This work is aimed at exploring the effects of UV-C irradiation on recombinant human γD-crystallin (HGDC). METHODS: Recombinant HGDC proteins were expressed in E. coli strain BL21(DE3) harboring plasmid pEHisHGDC and purified using chromatographic methods. The proteins were then exposed to UV-C light (λ(max)=254 nm, 15 W) at the intensity of 420, 800, or 1850 µW/cm(2). The UV-C-unexposed, supernatant fraction of UV-C-exposed, and re-dissolved precipitated fraction of UV-C exposed preparations were characterized by SDS-PAGE, turbidity measurement, CD spectroscopy, tryptophan fluorescence spectroscopy, acrylamide fluorescence quenching analysis, and sulfhydryl group measurements. RESULTS: The turbidity of the HGDC sample solution was found to be positively correlated with HGDC concentration, UV-C irradiation intensity, and UV-C irradiation duration. When exposed to UV-C, HGDC sample solutions became visibly turbid and a noticeable amount of larger protein particle, perceptible to the naked eye, was observed upon prolonged irradiation. The precipitated fraction of irradiated HGDC sample was found to be re-dissolved by guanidine hydrochloride. Electrophoresis, acrylamide fluorescence quenching, and spectroscopic analyses revealed differences in structures among the non-irradiated HGDC, the supernatant fraction of irradiated HGDC, and the re-dissolved precipitated fraction of irradiated HGDC. Through the use of L-cysteine, the measurements of sulfhydryl contents, and the reducing as well as non-reducing SDS-PAGE, our data further suggested that disulfide bond formation and/or cleavage probably play an important role in aggregation and/or precipitation of HGDC elicited by UV-C irradiation. CONCLUSIONS: Our findings highlight the close connections among disulfide bond cleavage and/or formation, intermolecular interactions, and the resultant formation of aggregates of HGDC induced by UV-C irradiation. The results from this research may not only contribute to the understanding of the environmental factors causing protein aggregation but also have implications for deciphering the molecular mechanism of cataractogenesis.
Assuntos
Proteínas Recombinantes/efeitos da radiação , Raios Ultravioleta , gama-Cristalinas/efeitos da radiação , Acrilamida/metabolismo , Precipitação Química/efeitos dos fármacos , Precipitação Química/efeitos da radiação , Dicroísmo Circular , Dissulfetos/metabolismo , Eletroforese em Gel de Poliacrilamida , Guanidina/farmacologia , Humanos , Peso Molecular , Nefelometria e Turbidimetria , Polimerização/efeitos dos fármacos , Polimerização/efeitos da radiação , Estrutura Quaternária de Proteína , Estrutura Secundária de Proteína , Estrutura Terciária de Proteína , Proteínas Recombinantes/química , Solubilidade/efeitos dos fármacos , Solubilidade/efeitos da radiação , Espectrometria de Fluorescência , Triptofano/metabolismo , gama-Cristalinas/químicaRESUMO
Proteins exposed to UV radiation are subject to irreversible photodamage through covalent modification of tryptophans (Trps) and other UV-absorbing amino acids. Crystallins, the major protein components of the vertebrate eye lens that maintain lens transparency, are exposed to ambient UV radiation throughout life. The duplicated beta-sheet Greek key domains of beta- and gamma-crystallins in humans and all other vertebrates each have two conserved buried Trps. Experiments and computation showed that the fluorescence of these Trps in human gammaD-crystallin is very efficiently quenched in the native state by electrostatically enabled electron transfer to a backbone amide [Chen et al. (2006) Biochemistry 45, 11552-11563]. This dispersal of the excited state energy would be expected to minimize protein damage from covalent scission of the excited Trp ring. We report here both experiments and computation showing that the same fast electron transfer mechanism is operating in a different crystallin, human gammaS-crystallin. Examination of solved structures of other crystallins reveals that the Trp conformation, as well as favorably oriented bound waters, and the proximity of the backbone carbonyl oxygen of the n - 3 residues before the quenched Trps (residue n), are conserved in most crystallins. These results indicate that fast charge transfer quenching is an evolved property of this protein fold, probably protecting it from UV-induced photodamage. This UV resistance may have contributed to the selection of the Greek key fold as the major lens protein in all vertebrates.
Assuntos
Cristalinas/química , Evolução Molecular , Cristalino/química , Dobramento de Proteína , Triptofano/química , Raios Ultravioleta/efeitos adversos , gama-Cristalinas/química , Cristalinas/genética , Cristalinas/efeitos da radiação , Cristalografia por Raios X , Transporte de Elétrons/genética , Transporte de Elétrons/efeitos da radiação , Humanos , Cristalino/lesões , Cristalino/efeitos da radiação , Mutagênese , Dobramento de Proteína/efeitos da radiação , Espectrometria de Fluorescência , Eletricidade Estática , Luz Solar/efeitos adversos , Triptofano/genética , Triptofano/efeitos da radiação , gama-Cristalinas/genética , gama-Cristalinas/efeitos da radiaçãoRESUMO
The eye lens Crystallin proteins are subject to UV irradiation throughout life, and the photochemistry of damage proceeds through the excited state; thus, their tryptophan (Trp) fluorescence lifetimes are physiologically important properties. The time-resolved fluorescence spectra of single Trps in human gammaD- and gammaS-Crystallins have been measured with both an upconversion spectrophotofluorometer on the 300 fs to 100 ps time scale, and a time correlated single photon counting apparatus on the 100 ps to 10 ns time scale, respectively. Three Trps in each wild type protein were replaced by phenylalanine, leading to single-Trp mutants: W68-only and W156-only of HgammaD- and W72-only and W162-only of HgammaS-Crystallin. These proteins exhibit similar ultrafast signatures: positive definite decay associated spectra (DAS) for 50-65 ps decay constants that indicate dominance of fast, heterogeneous quenching. The quenched population (judged by amplitude) of this DAS differs among mutants. Trps 68, 156 in human gammaD- and Trp72 in human gammaS-Crystallin are buried, but water can reach amide oxygen and ring HE1 atoms through narrow channels. QM-MM simulations of quenching by electron transfer predict heterogeneous decay times from 50-500 ps that agree with our experimental results. Further analysis of apparent radiative lifetimes allow us to deduce that substantial subpopulations of Trp are fully quenched in even faster (sub-300 fs) processes for several of the mutants. The quenching of Trp fluorescence of human gammaD- and gammaS-Crystallin may protect them from ambient light induced photo damage.
Assuntos
Triptofano/química , gama-Cristalinas/química , gama-Cristalinas/efeitos da radiação , Humanos , Fenilalanina/química , Fenilalanina/genética , Espectrometria de Fluorescência , Triptofano/genética , Raios Ultravioleta , gama-Cristalinas/genéticaRESUMO
In response to illumination by ultraviolet-A (UV-A) light, proteins in solid form are now known to display a visible blue fluorescence, ostensibly on account of excitation transitions of loosely-held electrons within peptide bond orbitals engaged in hydrogen bonding. Because the CO and NH atom groups in peptide bonds are generally engaged in extensive hydrogen bonding in globular proteins even in aqueous solution, one could argue that proteins in solution must also display this novel blue fluorescence. Here, using high concentrations to enhance detectability, two globular proteins, gamma-crystallin, and lysozyme, are shown to fluoresce visibly, exhibiting: (a) two excitation maxima, at approximately 315 nm and approximately 385 nm, (b) maximal emission at 425 nm in 100 mg/ml lysozyme and 465 nm in 100 mg/ml gamma-crystallin, (c) a time-resolved emission decay that is best fitted by a sum of three exponentials with lifetimes of 3.14, 0.46, and 9.08 ns, respectively, and comparable relative amplitudes of around 30-40 percent each, and (d) a weak CD spectrum displaying a positive band at approximately 385 nm and a negative band at approximately 465 nm. While the wavelength of maximal emission ((em)lambda(max)) in lysozyme is the same for all protein concentrations, the (em)lambda(max) of gamma-crystallin varies with protein concentration, suggesting a certain degree of conformation dependence.
Assuntos
Proteínas/química , Proteínas/efeitos da radiação , Animais , Bovinos , Dicroísmo Circular , Fluorescência , Ligação de Hidrogênio , Técnicas In Vitro , Muramidase/química , Muramidase/efeitos da radiação , Proteínas Recombinantes/química , Proteínas Recombinantes/efeitos da radiação , Soluções , Espectrometria de Fluorescência , Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz , Raios Ultravioleta , gama-Cristalinas/química , gama-Cristalinas/efeitos da radiaçãoRESUMO
Purpose: Cataract results from the formation of light-scattering precipitates due to point mutations or accumulated damage in the structural crystallins of the eye lens. Although excised cataracts are predominantly amorphous, in vitro studies show that crystallins are capable of adopting a variety of morphologies depending on the preparation method. Here we characterize thermal, pH-dependent, and UV-irradiated aggregates from wild-type human γS-crystallin (γS-WT) and its aggregation-prone variant, γS-G18V. Methods: Aggregates of γS-WT and γS-G18V were prepared under acidic, neutral, and basic pH conditions and held at 25°C or 37°C for 48 hours. UV-induced aggregates were produced by irradiation with a 355-nm laser. Aggregation and fibril formation were monitored via turbidity and thioflavin T (ThT) assays. Aggregates were characterized using intrinsic aromatic fluorescence, powder x-ray diffraction, and mass spectrometry. Results: γS-crystallin aggregates displayed different characteristics depending on the preparation method. γS-G18V produced a larger amount of detectable aggregates than did γS-WT and at less-extreme conditions. Aggregates formed under basic and acidic conditions yielded elevated ThT fluorescence; however, aggregates formed at low pH did not produce strongly turbid solutions. UV-induced aggregates produced highly turbid solutions but displayed only moderate ThT fluorescence. X-ray diffraction confirms amyloid character in low-pH samples and UV-irradiated samples, although the relative amounts vary. Conclusions: γS-G18V demonstrates increased aggregation propensity compared to γS-WT when treated with heat, acid, or UV light. The resulting aggregates differ in their ThT fluorescence and turbidity, suggesting that at least two different aggregation pathways are accessible to both proteins under the conditions tested.
Assuntos
Catarata , Conformação Proteica , gama-Cristalinas/química , Catarata/genética , Catarata/metabolismo , Temperatura Alta , Humanos , Concentração de Íons de Hidrogênio , Luz , Conformação Proteica/efeitos da radiação , Espectrometria de Fluorescência , Raios Ultravioleta , Difração de Raios X/métodos , gama-Cristalinas/genética , gama-Cristalinas/efeitos da radiaçãoRESUMO
Age related cataract is a major cause of visual loss worldwide that is a result of opacification of the eye lens proteins. One of the major reasons behind this deterioration is UV induced oxidative damage. The study reported here is focused on an investigation of the oxidative stress induced damage to γB-crystallin under UV exposure. Human γB-crystallin has been expressed and purified from E. coli. We have found that epicatechin gallate (ECG) has a higher affinity towards the protein compared to epigallocatechin (EGC). The in vitro study of UV irradiation under oxidative damage to the protein in the presence of increasing concentrations of GTPs is indicative of their effective role as potent inhibitors of oxidative damage. Docking analyses show that the GTPs bind to the cleft between the domains of human γB-crystallin that may be associated with the protection of the protein from oxidative damage.
Assuntos
Flavonoides/farmacologia , Estresse Oxidativo/efeitos dos fármacos , Substâncias Protetoras/farmacologia , Chá/química , Raios Ultravioleta/efeitos adversos , gama-Cristalinas/química , Escherichia coli/efeitos dos fármacos , Escherichia coli/efeitos da radiação , Humanos , Estresse Oxidativo/efeitos da radiação , gama-Cristalinas/efeitos dos fármacos , gama-Cristalinas/efeitos da radiaçãoRESUMO
The transparency of the human eye lens depends on the solubility and stability of the structural proteins of the eye lens, the crystallins. Although the mechanism of cataract formation is still unclear, it is believed to involve protein misfolding and/or aggregation of proteins due to the influence of several external factors such as ultraviolet (UV) radiation, low pH, temperature and exposure to chemical agents. In this article, we report the study of UV induced photo-damage (under oxidative stress) of recombinant human γB-crystallin in vitro in the presence of the major green tea polyphenol, (-)-epigallocatechin gallate (EGCG). We have shown that EGCG has the ability to protect human γB-crystallin from oxidative stress-induced photo-damage.
Assuntos
Catequina/análogos & derivados , Simulação de Acoplamento Molecular , Protetores contra Radiação/química , Análise Espectral , Raios Ultravioleta , gama-Cristalinas/química , Catequina/química , Catequina/farmacologia , Humanos , Simulação de Dinâmica Molecular , Estrutura Molecular , Oxirredução , Estresse Oxidativo , Ligação Proteica , Estabilidade Proteica , Protetores contra Radiação/farmacologia , Solubilidade , Raios Ultravioleta/efeitos adversos , gama-Cristalinas/efeitos dos fármacos , gama-Cristalinas/efeitos da radiaçãoRESUMO
The eye lens protein γD-crystallin contributes to cataract formation in the lens. In vitro experiments show that γD-crystallin has a high propensity to form amyloid fibers when denatured, and that denaturation by acid or UV-B photodamage results in its C-terminal domain forming the ß-sheet core of amyloid fibers. Here, we show that thermal denaturation results in sheet-like aggregates that contain cross-linked oligomers of the protein, according to transmission electron microscopy and SDS-PAGE. We use two-dimensional infrared spectroscopy to show that these aggregates have an amyloid-like secondary structure with extended ß-sheets, and use isotope dilution experiments to show that each protein contributes approximately one ß-strand to each ß-sheet in the aggregates. Using segmental (13) C labeling, we show that the organization of the protein's two domains in thermally induced aggregates results in a previously unobserved structure in which both the N-terminal and C-terminal domains contribute to ß-sheets. We propose a model for the structural organization of the aggregates and attribute the recruitment of the N-terminal domain into the fiber structure to intermolecular cross linking.
Assuntos
Amiloide/química , gama-Cristalinas/química , gama-Cristalinas/efeitos da radiação , Amiloide/efeitos da radiação , Humanos , Microscopia Eletrônica de Transmissão , Modelos Moleculares , Desnaturação Proteica , Isoformas de Proteínas/química , Isoformas de Proteínas/efeitos da radiação , Estrutura Secundária de Proteína , Espectroscopia de Infravermelho com Transformada de Fourier , TemperaturaRESUMO
Exposure to ultraviolet radiation (UVR) is a significant risk factor for age-related cataract, a disease of the human lens and the most prevalent cause of blindness in the world. Cataract pathology involves protein misfolding and aggregation of the primary proteins of the lens, the crystallins. Human γD-crystallin (HγD-Crys) is a major γ-crystallin in the nucleus of the human lens. We report here analysis of UVR-induced damage to HγD-Crys in vitro. Irradiation of solutions of recombinant HγD-Crys with UVA/UVB light produced a rise in solution turbidity due to polymerization of the monomeric crystallins into higher molecular weight aggregates. A significant fraction of this polymerized protein was covalently linked. Photoaggregation of HγD-Crys required oxygen and its rate was protein concentration and UVR dose dependent. To investigate the potential roles of individual tryptophan residues in photoaggregation, triple W:F mutants of HγD-Crys were irradiated. Surprisingly, despite reducing UVR absorbing capacity, multiple W:F HγD-Crys mutant proteins photoaggregated more quickly and extensively than wild type. The results reported here are consistent with previous studies that postulated that an energy transfer mechanism between the highly conserved pairs of tryptophan residues in HγD-Crys could be protective against UVR-induced photodamage.
Assuntos
Triptofano/química , Raios Ultravioleta , gama-Cristalinas/química , Cristalografia por Raios X , Humanos , Microscopia Eletrônica de Transmissão , Modelos Moleculares , Polímeros/química , gama-Cristalinas/efeitos da radiaçãoRESUMO
Age-onset cataracts are believed to be expedited by the accumulation of UV-damaged human γD-crystallins in the eye lens. Here we show with molecular dynamics simulations that the stability of γD-crystallin is greatly reduced by the conversion of tryptophan to kynurenine due to UV-radiation, consistent with previous experimental evidences. Furthermore, our atomic-detailed results reveal that kynurenine attracts more waters and other polar sidechains due to its additional amino and carbonyl groups on the damaged tryptophan sidechain, thus breaching the integrity of nearby dry center regions formed by the two Greek key motifs in each domain. The damaged tryptophan residues cause large fluctuations in the Tyr-Trp-Tyr sandwich-like hydrophobic clusters, which in turn break crucial hydrogen-bonds bridging two ß-strands in the Greek key motifs at the "tyrosine corner". Our findings may provide new insights for understanding of the molecular mechanism of the initial stages of UV-induced cataractogenesis.