IgE antibodies from schistosomiasis patients to recognize epitopes in potato apyrase
Rev. Soc. Bras. Med. Trop
; 52: e20180139, 2019. graf
Article
em En
| LILACS
| ID: biblio-1041506
Biblioteca responsável:
BR1.1
ABSTRACT
Abstract INTRODUCTION:
High percentages of structural identity and cross-immunoreactivity have been reported between potato apyrase and Schistosoma mansoni ATP diphosphohydrolase (SmATPDases) isoforms, showing the existence of particular epitopes shared between these proteins.METHODS:
Potato apyrase was employed using ELISA, western blot, and mouse immunization methods to verify IgE reactivity.RESULTS:
Most of the schistosomiasis patient's (75%) serum was seropositive for potato apyrase and this protein was recognized using western blotting, suggesting that parasite and plant proteins share IgE-binding epitopes. C57BL/6 mice immunized with potato apyrase showed increased IgE antibody production.CONCLUSIONS:
Potato apyrase and SmATPDases have IgE-binding epitopes.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
LILACS
Assunto principal:
Apirase
/
Schistosoma mansoni
/
Esquistossomose mansoni
/
Solanum tuberosum
/
Imunoglobulina E
/
Anticorpos Anti-Helmínticos
/
Epitopos
Limite:
Animals
Idioma:
En
Revista:
Rev. Soc. Bras. Med. Trop
Assunto da revista:
MEDICINA TROPICAL
Ano de publicação:
2019
Tipo de documento:
Article
País de afiliação:
Brasil